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A unified pre-training and adaptation framework for combinatorial optimization on graphs

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Science China Mathematics 2024年第6期67卷 1439-1456页

作者： Ruibin Zeng minglong Lei Lingfeng Niu Lan Cheng School of Computer Science and Technology University of Chinese Academy of SciencesBeijing100049China Faculty of Information Technology Beijing University of TechnologyBeijing100124China School of Economics and Management University of Chinese Academy of SciencesBeijing100190China School of Mathematics and Statistics Central South UniversityChangsha410075China

Combinatorial optimization(CO)on graphs is a classic topic that has been extensively studied across many scientific and industrial fields.Recently,solving CO problems on graphs through learning methods has attracted great attention.Advanced deep learning methods,e.g.,graph neural networks(GNNs),have been used to effectively assist the process of solving COs.However,current frameworks based on GNNs are mainly designed for certain CO problems,thereby failing to consider their transferable and generalizable abilities among different COs on graphs.Moreover,simply using original graphs to model COs only captures the direct correlations among objects,which does not consider the mathematical logicality and properties of COs.In this paper,we propose a unified pre-training and adaptation framework for COs on graphs with the help of the maximum satisfiability(Max-SAT)problem.We first use Max-SAT to bridge different COs on graphs since they can be converted to Max-SAT problems represented by standard formulas and clauses with logical information.Then we further design a pre-training and domain adaptation framework to extract the transferable and generalizable features so that different COs can benefit from them.In the pre-training stage,Max-SAT instances are generated to initialize the parameters of the model.In the fine-tuning stage,instances from CO and Max-SAT problems are used for adaptation so that the transferable ability can be further improved.Numerical experiments on several datasets show that features extracted by our framework exhibit superior transferability and Max-SAT can boost the ability to solve COs on graphs.

关键词： combinatorial optimization graph neural networks domain adaptation maximum satisfiability problem

Enhanced gas production and CO_(2) storage in hydrate-bearing sediments via pre-depressurization and rapid CO_(2) injection

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Chinese Journal of Chemical Engineering 2024年第3期67卷 126-134页

作者： Hongnan Chen Yifei Sun Bojian Cao minglong Wang Ming Wang Jinrong Zhong Changyu Sun Guangjin Chen State Key Laboratory of Heavy Oil Processing China University of PetroleumBeijing 102249China School of Chemistry and Chemical Engineering Changsha University of Science&TechnologyChangsha 410114China

Carbon emission reduction and clean energy development are urgent demands for mankind in the coming decades.Exploring an efficient CO_(2) storage method can significantly reduce CO_(2) emissions in the short term.In this study,we attempted to construct sediment samples with different residual CH_(4) hydrate amounts and reservoir conditions,and then investigate the potentials of both CO_(2) storage and enhanced CH_(4) recovery in depleted gas hydrate deposits in the permafrost and ocean zones,respectively.The results demonstrate that CO_(2) hydrate formation rate can be significantly improved due to the presence of residual hydrate seeds;However,excessive residual hydrates in turn lead to the decrease in CO_(2) storage efficiency.Affected by the T-P conditions of the reservoir,the storage amount of liquid CO_(2) can reach 8 times that of gaseous CO_(2),and CO_(2) stored in hydrate form reaches 2-4 times.Additionally,we noticed two other advantages of this method.One is that CO_(2) injection can enhance CH_(4) recovery rate and increases CH_(4) recovery by 10%-20%.The second is that hydrate saturation in the reservoir can be restored to 20%-40%,which means that the solid volume of the reservoir avoids serious shrinkage.Obviously,this is crucial for protecting the goaf stability.In summary,this approach is greatly promising for high-efficient CO_(2) storage and safe exploitation of gas hydrate.

关键词： Hydrate Depressurization CO_(2) storage CH_(4) production Reservoir remediation

The E3 ubiquitin ligase CSIT1 regulates critical sterility-inducing temperature by ribosome-associated quality control to safeguard two-line hybrid breeding in rice

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Molecular Plant 2023年第10期16卷 1695-1709页

作者： Guoqing Peng minglong liu Liya Zhu Wenlong Luo Qinghua Wang Mumei Wang Huiqiong Chen Ziliang Luo Yueping Xiao Yongjie Zhang Haona Hong Zhenlan liu Lingyan Zhou Guoqiang Guo Yingxiang Wang Chuxiong Zhuang Hai Zhou State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangdong Laboratory for Lingnan Modern AgricultureKey Laboratory for Enhancing Resource Use Efficiency of Crops in South ChinaMinistry of Agriculture and Rural AffairsCollege of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China College of Agriculture&Biology Zhongkai University of Agriculture and EngineeringGuangzhou 510225China Guangdong Key Laboratory for New Technology Research of Vegetables Vegetable Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China Agronomy Department University of FloridaGainesvilleFL 32610USA Hengyang Academy of Agricultural Sciences Hengyang 421101China

Two-line hybrid breeding can fully utilize heterosis in crops.In thermo-sensitive genic male sterile(TGMS)lines,low critical sterility-inducing temperature(CSIT)is vital to safeguard the production of two-line hybrid seeds in rice(Oryza sativa),but the molecular mechanism determining CSIT is unclear.Here,we report the cloning of CSIT1,which encodes an E3 ubiquitin ligase,and show that CSIT1 modulates the CSIT of thermo-sensitive genic male sterility 5(tms5)-based TGMS lines through ribosome-associated quality control(RQC).Biochemical assays demonstrated that CSIT1 binds to the 80S ribosomes and ubiquitinates abnormal nascent polypeptides for degradation in the RQC process.Loss of CSIT1 function inhibits the possible damage of tms5 to the ubiquitination system and protein translation,resulting in enhanced accumulation of anther-related proteins such as catalase to suppress abnormal accumulation of reactive oxygen species and premature programmed cell death in the tapetum,thereby leading to a much higher CSIT in the tms5-based TGMS lines.Taken together,our findings reveal a regulatory mechanism of CSIT,providing new insights into RQC and potential targets for future two-line hybrid breeding.

关键词： hybrid breeding thermo-sensitive genic male sterility critical sterility-inducing temperature ribosome-associated quality control programmed cell death

Wild soybean(Glycine soja)transcription factor GsWRKY40 plays positive roles in plant salt tolerance

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The Crop Journal 2024年第3期12卷 766-775页

作者： minglong Li Man Xue Huiying Ma Peng Feng Tong Chen Xiaohuan Sun Qiang Li Xiaodong Ding Shuzhen Zhang Jialei Xiao Key Laboratory of Agricultural Biological Functional Genes Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Key Laboratory of Soybean Biology of Chinese Education Ministry Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Department of Plant Science School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China College of Life Science Leshan Normal UniversityLeshan 614000SichuanChina Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences Mudanjiang 157401HeilongjiangChina

Wild soybean(Glycine soja),a relative of cultivated soybean,shows high adaptability to adverse environmental conditions.We identified and characterized a wild soybean transcription factor gene,GsWRKY40,that promotes plant salt stress.GsWRKY40 was highly expressed in wild soybean roots and was up-regulated by salt treatment.GsWRKY40 was localized in nucleus and demonstrated DNA-binding activities but without transcriptional activation.Mutation and overexpression of GsWRKY40 altered salt tolerance of Arabidopsis plants.To understand the molecular mechanism of GsWRKY40 in regulating plant salt resistance,we screened a cDNA library and identified a GsWRKY40 interacting protein GsbHLH92 by using yeast two-hybrid approach.The physical interaction of GsWRKY40 and GsbHLH92 was confirmed by co-immunoprecipitation(co-IP),GST pull-down,and bimolecular fluorescence complementation(BiFC)techniques.Intriguingly,co-overexpression of GsWRKY40 and GsbHLH92 resulted in higher salt tolerance and lower ROS levels than overexpression of GsWRKY40 or GsbHLH92 in composite soybean plants,suggesting that GsWRKY40 and GsbHLH92 may synergistically regulate plant salt resistance through inhibiting ROS production.qRT-PCR data indicated that the expression level of GmSPOD1 gene encoding peroxidase was cooperatively regulated by GsWRKY40 and GsbHLH92,which was confirmed by using a dual luciferase report system and yeast one-hybrid experiment.Our study reveals a pathway that GsWRKY40 and GsbHLH92 collaboratively up-regulate plant salt resistance through impeding GmSPOD1 expression and reducing ROS levels,providing a novel perspective on the regulatory mechanisms underlying plant tolerance to abiotic stresses.

关键词： Wild soybean Transcription factor Salt stress ROS

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基于分层融合策略和上下文信息嵌入的多模态情绪识别

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北京大学学报（自然科学版） 2024年第3期60卷 393-402页

作者：孙明龙欧阳纯萍刘永彬任林南华大学计算机学院衡阳421200

现有的多模态融合策略大多将不同模态特征进行简单拼接,忽略了针对单个模态固有特点的个性化融合需求。同时,在情绪识别阶段,独立地看待单个话语的情绪而不考虑其在前后话语语境下的情绪状态,可能导致情绪识别错误。为了解决上述问题,... 详细信息

现有的多模态融合策略大多将不同模态特征进行简单拼接,忽略了针对单个模态固有特点的个性化融合需求。同时,在情绪识别阶段,独立地看待单个话语的情绪而不考虑其在前后话语语境下的情绪状态,可能导致情绪识别错误。为了解决上述问题,提出一种基于分层融合策略和上下文信息嵌入的多模态情绪识别方法,通过分层融合策略,采用层次递进的方式,依次融合不同的模态特征,以便减少单个模态的噪声干扰并解决不同模态间表达不一致的问题。该方法还充分利用融合后模态的上下文信息,综合考虑单个话语在上下文语境中的情绪表示,以便提升情绪识别的效果。在二分类情绪识别任务中,该方法的准确率比SOTA模型提升1.54%。在多分类情绪识别任务中,该方法的F1值比SOTA模型提升2.79%。

关键词：分层融合噪声干扰上下文信息嵌入

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Physiological Analysis of Drought Resistance of Seven Cultivars Spring Wheat in Northern Regions of China

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Journal of Northeast Agricultural University(English Edition) 2024年第2期31卷 1-12页

作者： Gao Fengmei Zhang Fuzhi Zhao Yuanling Li Tie Li Dongmei Sun minglong Zhang Qichang liu Ningtao Sun Dan Tian Chao Sun Lianfa Institute of Crop Resources Heilongjiang Academy of Agricultural SciencesHarbin 150086China College of Art and Design Harbin Institute of Information EngineeringHarbin 150431China

In order to determine the physiological mechanism of drought resistance of northern wheat in China,six drought resistant wheat and one sensitivity to drought wheat were planted in pots.They were subjected to drought treatment and normal water when the plants grew to the 3-leaf stage.Samples were collected at 10,20,30,and 40 days after the drought treatment,respectively.The electrical conductivity,photosynthetic parameters,chlorophyll fluorescence parameters,sugar content,proline content,protein content,and active oxygen scavenging enzyme activity of the plants were detected,and the agronomic traits of the wheat varieties were investigated at maturity.The results indicated that the phenotype and yield-related factors of Darkhan 144 changed little under the drought stress.The relative electrical conductivity of Kefeng 6 and Darkhan 166 was lower under the drought stress,and their cell membrane was less damaged.The Darkhan 144 and Darkhan 166 had higher drought resistance coefficients,and were the wheat varieties with stronger drought resistance.However,the physiological mechanisms of drought resistance of these three wheat were different:Darkhan 144 maintained a higher photosynthetic activity under the drought stress;Darkhan 166 maintained a higher protein content,photosynthetic activity and active oxygen scavenging enzyme activity.In addition,other drought-resistant varieties Kefeng 6,Kefeng 10 and Longmai 26 had a higher content of osmoregulatory substances under the drought stress.

关键词： wheat tolerance drought physiological mechanism

Methylesterification of cell-wall pectin controls the diurnal flower-opening times in rice

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Molecular Plant 2022年第6期15卷 956-972页

作者： Mumei Wang Xiaopei Zhu Guoqing Peng minglong liu Shuqing Zhang Minghao Chen Shitang Liao Xiaoying Wei Peng Xu Xiyu Tan Fangping Li Zhichuan Li Li Deng Ziliang Luo Liya Zhu Shuai Zhao Dagang Jiang Jing Li Zhenlan liu Xianrong Xie Shaokui Wang Aimin Wu Chuxiong Zhuang Hai Zhou Guangdong Laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesCollege of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642China Agronomy Department University of FloridaGainesvilleFL 32610USA State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou 510642China

Flowers are the core reproductive organ of plants, and flowering is essential for cross-pollination. Diurnal flower-opening time is thus a key trait influencing reproductive isolation, hybrid breeding, and thermostability in plants. However, the molecular mechanisms controlling this trait remain unknown. Here, we report that rice Diurnal Flower Opening Time 1 (DFOT1) modulates pectin methylesterase (PME) activity to regulate pectin methylesterification levels of the lodicule cell walls, which affect lodicule swelling to control diurnal flower-opening time. DFOT1 is specifically expressed in the lodicules, and its expression gradually increases with the approach to flowering but decreases with flowering. Importantly, a knockout of DFOT1 showed earlier diurnal flower opening. We demonstrate that DFOT1 interacts directly with multiple PMEs to promote their activity. Knockout of PME40 also resulted in early diurnal flower opening, whereas overexpression of PME42 delayed diurnal flower opening. Lower PME activity was observed to be associated with higher levels of pectin methylesterification and the softening of cell walls in lodicules, which contribute to the absorption of water by lodicules and cause them to swell, thus promoting early diurnal flower opening. Higher PME activity had the opposite effect. Collectively, our work uncovers a molecular mechanism underlying the regulation of diurnal flower-opening time in rice, which would help reduce the costs of hybrid breeding and improve the heat tolerance of flowering plants by avoiding higher temperatures at anthesis.

关键词： rice hybrid breeding flowering diurnal flower-opening times cell wall pectin methylesterase

基于防水粘结层附着力的桥面铺装层间粘结强度和抗剪强度预测

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新型建筑材料 2024年第2期51卷 122-127页

作者：刘明龙中铁建重庆投资集团有限公司重庆401122

针对目前水泥混凝土复合式桥面铺装层间结合效果有损评价方法存在的弊端,通过室内试验建立特定桥面铺装结构和材料条件下组合结构的层间粘结强度和层间剪切强度与附着力之间的函数关系,同时求得温度修正系数;根据测得的工程现场防水粘... 详细信息

针对目前水泥混凝土复合式桥面铺装层间结合效果有损评价方法存在的弊端,通过室内试验建立特定桥面铺装结构和材料条件下组合结构的层间粘结强度和层间剪切强度与附着力之间的函数关系,同时求得温度修正系数;根据测得的工程现场防水粘结层的附着力计算得到组合结构的层间粘结强度和抗剪强度,进而换算成20℃条件下的强度标准值;并参照相关规范对实际工程层间结合效果进行质量评定。结果表明,防水粘结层对水泥混凝土的附着力与组合结构的层间粘结强度及抗剪强度存在较强的线性正相关,且强度预测值与实测值的误差基本都在10%以内;强度预测值换算成标准值后可直接借助规范进行层间结合效果评价。

关键词：附着力桥面铺装防水粘结层粘结强度抗剪强度预测

粗糙度和金属间化合物粒子对A6111铝合金表面腐蚀的影响

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稀有金属材料与工程 2024年第5期53卷 1252-1261页

作者：康明龙邓运来雷金琴中南大学轻合金研究院湖南长沙410083 中南大学有色金属材料科学与工程教育部重点实验室湖南长沙410083 东北轻合金有限责任公司黑龙江哈尔滨150060

钎焊铝蜂窝夹层结构板常用于高速列车的地板和船舶的甲板。其中四层铝复合板(4343/3003/6111/3003)作为该蜂窝结构的面板常暴露于腐蚀性环境中。经过成分优化的6111铝合金作为四层铝复合板的主要支撑层材料,其组织结构和表面状态对腐蚀... 详细信息

钎焊铝蜂窝夹层结构板常用于高速列车的地板和船舶的甲板。其中四层铝复合板(4343/3003/6111/3003)作为该蜂窝结构的面板常暴露于腐蚀性环境中。经过成分优化的6111铝合金作为四层铝复合板的主要支撑层材料,其组织结构和表面状态对腐蚀性能有很大影响。采用不同粒度的砂纸磨削6111铝合金,研究了不同程度磨削后6111铝合金的微观组织和表面腐蚀行为。结果表明:6111合金中的AlFeSi(Mn,Cu)相的电位高于基体电位,这种相作为阴极与相邻基体形成多级体系,加剧了表面腐蚀。同时,较光滑的表面具有更好的耐腐蚀性。当表面粗糙度从18.03μm降至0.92μm时,表面几何体积由0.629 mm3减少至0.029 mm3,金属间化合物AlFeSi(Mn,Cu)粒子的平均数量由1631 mm-2减少至917 mm-2,其面积分数由3.93%降至0.92%。相应地,平均腐蚀深度由237μm降至95μm。

关键词： 6111铝合金表面粗糙度金属间化合物颗粒耐腐蚀性电化学测试