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The transcriptional regulators of virulence for Pseudomonas aeruginosa:Therapeutic opportunity and preventive potential of its clinical infections

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Genes & Diseases 2023年第5期10卷 2049-2063页

作者： Xiaolong Shao Chunyan Yao Yiqing Ding Haiyan Hu Guoliang Qian mingliang he Xin Deng College of Plant Protection Laboratory of Plant ImmunityKey Laboratory of Integrated Management of Crop Diseases and PestsNanjing Agricultural UniversityNanjingJiangsu 210095China Department of Biomedical Sciences City University of Hong KongKowloon TongHong Kong SARChina School of Pharmaceutical Sciences Sun Yat-Sen UniversityUniversity TownGuangzhouGuangdong 510006China Tung Biomedical Sciences Centre City University of Hong KongKowloon TongHong Kong SARChina Shenzhen Research Institute City University of Hong KongShenzhenGuangdong 518057China

In Pseudomonas aeruginosa(P.aeruginosa),transcription factors(TFs)are important mediators in the genetic regulation of adaptability and pathogenicity to respond to multiple environmental stresses and host defences.The P.aeruginosa genome harbours 371 putative TFs;of these,about 70 have been shown to regulate virulence-associated phenotypes by binding to the promoters of their target genes.Over the past three decades,several techniques have been applied to identify TF binding sites on the P.aeruginosa genome,and an atlas of TF binding patterns has been mapped.The virulence-associated regulons of TFs show complex crosstalk in P.aeruginosa's regulatory network.In this review,we summarise the recent literature on TF regulatory networks involved in the quorum-sensing system,biofilm formation,pyocyanin synthesis,motility,the type III secretion system,the type VI secretion system,and oxidative stress responses.We discuss future perspectives that could provide insights and targets for preventing clinical infections caused by P.aeruginosa based on the global regulatory network of transcriptional regulators.

关键词： Crosstalk Pseudomonas aeruginosa Regulatory network Transcriptional regulators Virulence

bZIP71 delays flowering by suppressing Ehd1 expression in rice

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Journal of Integrative Plant Biology 2022年第7期64卷 1352-1363页

作者： Xiufeng Li Xiaojie Tian mingliang he Xinxin Liu Zhiyong Li Jiaqi Tang Enyang Mei Min Xu Yingxiang Liu Zhenyu Wang Qingjie Guan Wei Meng Jun Fang Jian Zhang Qingyun Bu Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design Breedingthe Chinese Academy ofSciencesHarbin 150081China University of Chinese Academy of Sciences Beijing 100049China College of Life Science Northeast Forestry UniversityHarbin 150040China Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040China State Key Lab of Rice Biology China National Rice Research InstituteHangzhou 311400China The Innovative Academy of Seed Design the Chinese Academy of SciencesBeijing 100101China

Flowering time is a fundamental factor determining the global distribution and final yield of rice(Oryza sativa).Although diverse flowering time genes have been reported in this crop,the transcriptional regulation of its key flowering genes are poorly understood.here,we report that a basic leucine zipper transcription factor,bZIP71,functions as a flowering repressor.The overexpression of bZIP71 delays flowering,while the bzip71 mutant flowers early in both long-day and short-day conditions.A genetic analysis showed that the regulation of flowering by bZIP71 might be independent of heading date 2(Hd2),Hd4,and Hd5.Importantly,bZIP71 directly associates with the Early heading date 1(Ehd1)promoter and represses its transcription,and genetically the function of bZIP71 is impaired in the ehd1 mutant.Moreover,bZIP71 interacts with major components of polycomb repressive complex 2(PRC2),SET domain group protein 711(SDG711),and Fertilization independent endosperm 2(FIE2),through which bZIP71 regulates the H3K27me3 level of Ehd1.Taken together,we present a transcriptional regulatory mechanism in which bZIP71 enhances the H3K27me3 level of Ehd1 and transcriptionally represses its expression,which not only offers a novel insight into a flowering pathway,but also provides a valuable putative target for the genetic engineering and breeding of elite rice cultivars.

关键词： flowering time H3K27me3 rice transcriptional regulation

Oryza sativa mediator subunit OsMED25 interacts with OsBZR1 to regulate brassinosteroid signaling and plant architecture in rice

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Journal of Integrative Plant Biology 2020年第6期62卷 793-811页

作者： Yuekun Ren Xiaojie Tian Shuyu Li Enyang Mei mingliang he Jiaqi Tang Min Xu Xiufeng Li Zhenyu Wang Chuanyou Li Qingyun Bu Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design BreedingChinese Academy of SciencesHarbin 150081China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Brassinosteroids(BRs)are plant-specific steroid hormones which regulate plant growth,development,and adaptation.Transcriptional regulation plays key roles in plant hormone signaling.A mediator can serve as a bridge between gene-specific transcription factors and the RNA polymerase machinery,functioning as an essential component in regulating the transcriptional process.However,whether a mediator is involved in BR signaling is unknown.here,we discovered that Oryza sativa mediator subunit 25(Os MED25)is an important regulator of rice BR signaling.Phenotypic analyses showed that the Os MED25-RNAi and osmed25 mutant presented erect leaves,as observed in BR-deficient mutants.In addition,the Os MED25-RNAi and osmed25 mutant exhibited decreased BR sensitivity.Genetic analysis indicated that Os MED25-RNAi could suppress the enhanced BR signaling phenotype of Osbzr1-D.Further biochemical analysis showed that Os MED25 interacts with Os BZR1 in vivo,and Os MED25 is enriched on the promoter of Os BZR1 target genes.RNA sequencing analysis indicated that Os MED25 affects the expression of approximately 45%of Os BZR1-regulated genes and mainly functions as a corepressor of Os BZR1.Together,these findings revealed that Os MED25 regulates rice BR signaling by interacting with Os BZR1 and modulating the expression of Os BZR1 target genes,thus expanding our understanding of the roles of mediators in plant hormone signaling.

关键词： plant Together analysis

OsMKKK70 regulates grain size and leaf angle in rice through the OsMKK4-OsMAPK6-OsWRKY53 signaling pathway

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Journal of Integrative Plant Biology 2021年第12期63卷 2043-2057页

作者： Zhiqi Liu Enyang Mei Xiaojie Tian mingliang he Jiaqi Tang Min Xu Jiali Liu Lu Song Xiufeng Li Zhenyu Wang Qingjie Guan Qijiang Xu Qingyun Bu Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040China College of Life Science Northeast Forestry UniversityHarbin 150040China Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design BreedingCASHarbin 150081China University of Chinese Academy of Sciences Beijing 100049China School of Laboratory Medicine Youjiang Medical University for NationalitiesBaise 533000China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Grain size and leaf angle are key agronomic traits that determine final yields in rice.However,the underlying molecular mechanisms are not well understood.here we demonstrate that the Oryza sativa Mitogen Activated Protein Kinase Kinase Kinase OsMKKK70 regulates grain size and leaf angle in rice.Overexpressing OsMKKK70 caused plants to produce longer seeds.The osmkkk62/70 double mutant and the osmkkk55/62/70 triple mutant displayed significantly smaller seeds and a more erect leaf angle compared to the wild type,indicating that OsMKKK70 functions redundantly with its homologs Os MKKK62 and Os MKKK55.Biochemical analysis demonstrated that OsMKKK70 is an active kinase and that OsMKKK70 interacts with Os MKK4 and promotes Os MAPK6 phosphorylation.In addition,the osmkkk62/70 double mutant showed reduced sensitivity to Brassinosteroids(BRs).Finally,overexpressing constitutively active Os MKK4,Os MAPK6,and Os WRKY53 can partially complement the smaller seed size,erect leaf,and BR hyposensitivity of the osmkkk62/70 double mutant.Taken together,these findings suggest that OsMKKK70 might regulate grain size and leaf angle in rice by activating Os MAPK6 and that OsMKKK70,Os MKK4,Os MAPK6,and Os WRKY53 function in a common signaling pathway that controls grain shape and leaf angle.

关键词： Brassinosteroids MAPK signaling OsMKKK70 rice seed size

Experimental study on reactions between alkaline basaltic melt and orthopyroxenes: constraints on the evolution of lithospheric mantle in the North China Craton

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Acta Geochimica 2024年第2期43卷 354-365页

作者： Hanqi he mingliang Wang Hongfeng Tang Key Laboratory for High Temperature and High Pressure Study of the Earth’s Interior Institute of GeochemistryChinese Academy of SciencesGuiyang 550081China University of Chinese Academy of Sciences Beijing 100049China School of Resource and Civil Engineering Suzhou UniversitySuzhou 234000AnhuiChina

The experimental results of the reactions between an alkaline basaltic melt and mantle orthopyroxenes under high-temperature and high-pressure conditions of 1300–1400℃ and 2.0–3.0 GPa using a six-anvil apparatus are reported in this paper.The reactions are proposed to simulate the interactions between melts from the asthenospheric mantle and the lithospheric mantle.The starting melt in the experiments was made from the alkaline basalt occurring in Fuxin,Liaoning Province,and the orthopyroxenes were separated from the mantle xenoliths in Damaping,hebei Province.The results show that clinopyroxenes were formed in all the reactions between the alkaline basaltic melt and orthopyroxenes under the studied P–T conditions.The formation of clinopyroxene in the reaction zone is mainly controlled by dissolution–crystallization,and the chemical compositions of the reacted melt are primarily infl uenced by the diff usion eff ect.Temperature is the most important parameter controlling the reactions between the melt and orthopyroxenes,which has a direct impact on the melting of orthopyroxenes and the diff usion of chemical components in the melt.Temperature also directly controls the chemical compositions of the newly formed clinopyroxenes in the reaction zone and the reacted melt.The formation of clinopyroxenes from the reactions between the alkaline basaltic melt and orthopyroxenes can result in an increase of CaO and Al_(2)O_(3) contents in the rocks containing this mineral.Therefore,the reactions between the alkaline basaltic melt from the asthenospheric mantle and orthopyroxenes from the lithospheric mantle can lead to the evolution of lithospheric mantle in the North China Craton from refractory to fertile with relatively high CaO and Al 2 O 3 contents.In addition,the reacted melts in some runs were transformed from the starting alkaline basaltic into tholeiitic after reactions,indicating that tholeiitic magma could be generated from alkaline basaltic one via reactions between the latter and orthopyroxene.

关键词： Alkaline basaltic melt Orthopyroxene Melt–mineral reaction High-temperature and high-pressure experiment Genesis of basalt Evolution of lithospheric mantle in the North China Craton

Accelerated Primal-Dual Projection Neurodynamic Approach With Time Scaling for Linear and Set Constrained Convex Optimization Problems

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IEEE/CAA Journal of Automatica Sinica 2024年第6期11卷 1485-1498页

作者： You Zhao Xing he mingliang Zhou Tingwen Huang Chongqing Key Laboratory of Nonlinear Circuits and Intelligent Information Processing College of Electronic and Information EngineeringSouthwest UniversityChongqing 400715China School of Computer Science Chongqing UniversityChongqing 400044China Department of Mathematics Texas A&M University at QatarDoha 23874Qatar IEEE

The Nesterov accelerated dynamical approach serves as an essential tool for addressing convex optimization problems with accelerated convergence rates.Most previous studies in this field have primarily concentrated on unconstrained smooth con-vex optimization problems.In this paper,on the basis of primal-dual dynamical approach,Nesterov accelerated dynamical approach,projection operator and directional gradient,we present two accelerated primal-dual projection neurodynamic approaches with time scaling to address convex optimization problems with smooth and nonsmooth objective functions subject to linear and set constraints,which consist of a second-order ODE(ordinary differential equation)or differential conclusion system for the primal variables and a first-order ODE for the dual vari-ables.By satisfying specific conditions for time scaling,we demonstrate that the proposed approaches have a faster conver-gence rate.This only requires assuming convexity of the objective function.We validate the effectiveness of our proposed two accel-erated primal-dual projection neurodynamic approaches through numerical experiments.

关键词： Accelerated projection neurodynamic approach lin-ear and set constraints projection operators smooth and nonsmooth convex optimization time scaling.

基于转录组测序分析SOX18在湖羊毛囊毛乳头细胞中的功能

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畜牧兽医学报 2024年第6期55卷 2409-2420页

作者：何明亮吕晓阳蒋永清宋正海王叶青杨会国王善禾孙伟扬州大学动物科学技术学院扬州225009 教育部农业与农产品安全国际联合实验室扬州225009 江苏省普通高校家畜种质资源与遗传改良国际联合研究实验室扬州225009 浙江省农业科学院杭州310021 苏州市吴中区东山动物防疫站苏州215107 苏州太湖东山湖羊产业发展有限公司苏州215107 新疆畜牧科学院乌鲁木齐830011

旨在初步探究SOX18基因在湖羊毛囊毛乳头细胞中的功能。本研究首先采用免疫荧光染色试验对从湖羊毛囊中分离的细胞进行鉴定,然后构建SOX18基因过表达载体并通过qRT-PCR和Western blot检测其过表达效率。将经过鉴定且生长状态良好的毛乳... 详细信息

旨在初步探究SOX18基因在湖羊毛囊毛乳头细胞中的功能。本研究首先采用免疫荧光染色试验对从湖羊毛囊中分离的细胞进行鉴定,然后构建SOX18基因过表达载体并通过qRT-PCR和Western blot检测其过表达效率。将经过鉴定且生长状态良好的毛乳头细胞分为两组,分别转染SOX18过表达载体和空载质粒,每组3个重复。采用Trizol法提取6个样本的总RNA并构建cDNA文库,利用Illumina Novaseq6000平台进行测序,然后对样本相关性和差异表达基因进行分析,并对差异表达基因进行GO功能分类和KEGG通路注释,寻找SOX18基因调控的相关候选基因和通路。为确认转录组数据的准确性,随机选取9个差异表达基因进行qRT-PCR验证。免疫荧光结果显示,毛乳头相关基因在所分离的细胞中高表达,表明所分离的毛乳头细胞纯度高。qRT-PCR和Western blot检测发现,SOX18基因过表达能够增加毛乳头细胞中SOX18的mRNA和蛋白水平,表明构建的SOX18基因过表达载体可用于后续试验。转录组分析结果表明样本间相关性好,SOX18过表达组和对照组相比共发现157个基因差异表达,其中103个基因在SOX18过表达后上调,54个基因下调,qRT-PCR分析表明转录组数据准确性高。GO功能分析显示,一些差异基因富集于细胞进展和毛囊发育过程。KEGG富集分析表明,一些差异表达基因富集于细胞增殖和毛囊发育相关信号通路。GO功能分析和KEGG富集分析表明SOX18在毛乳头细胞的增殖和毛囊发育过程中起作用。本研究通过转录组测序分析发现SOX18可能通过影响细胞增殖和毛囊发育相关的基因和信号通路来影响毛乳头细胞增殖和毛囊生长发育,研究结果为解析SOX18基因在湖羊毛乳头细胞和毛囊中的分子调控机制提供了理论基础。

关键词：转录组测序 SOX18 毛囊毛乳头细胞湖羊

动态IPT系统后级稳压器的小信号模型修正和改进控制策略

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磁性元件与电源 2024年第5期 174-182页

作者：殷金安陈乾宏尹艳浩何明亮刘立东南京航空航天大学南京210016 横店集团东磁股份有限公司东阳322118

动态无线供电技术具有无磨损、维护成本低等优点,在自动化分栋系统中具有良好的应用价值。论文针对单轨动态无线供电系统,在考虑整流桥前的电流断续及副边谐振电路寄生参数的影响下,分析了整流电压与负载的关系,指出因负载变化导致的整... 详细信息

动态无线供电技术具有无磨损、维护成本低等优点,在自动化分栋系统中具有良好的应用价值。论文针对单轨动态无线供电系统,在考虑整流桥前的电流断续及副边谐振电路寄生参数的影响下,分析了整流电压与负载的关系,指出因负载变化导致的整流电压大范围波动给后级稳压器的建模和闭环参数设计带来了挑战。为使后级稳压器小信号模型描述的更为准确,论文考虑了副边侧谐振电路的影响,引入幅值/频率修正系数,对后级稳压器小信号模型的低频段增益和中频段谐振峰频率进行了修正。在此基础上,为进一步提高系统的变负载动态响应能力,论文提出加入整流电压前馈环节。仿真与实验结果验证了理论分析的正确性和所提方案的有效性。

关键词：动态无线电能传输寄生参数整流电压小信号模型控制策略

Multifunction-oriented high-mobility polymer semiconductors

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National Science Review 2024年第3期11卷 16-19页

作者： mingliang Zhu Yunlong Guo Yunqi Liu Beijing National Laboratory for Molecular Sciences Organic Solids Laboratory Institute of Chemistry Chinese Academy of Sciences School of Chemical Sciences University of Chinese Academy of Sciences

The discovery of conductive polyacetylene has initiated interdisciplinary research into condensed matter physics and synthetic chemistry in organic electronics. Unlike their inorganic and small-molecule counterparts, polymer semiconductors possess the inherent advantages of facile solution processing,large-area manufacturing, excellent mechanical strength and intrinsic flexibility.

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