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Effect of damage zone around borehole on carbon dioxide injection promoted gas extraction in soft and low-permeability coal seam

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Frontiers of Earth Science 2023年第3期17卷 776-787页

作者： Lijun ZHOU Xihua ZHOU Gang BAI Xianlin LI mingkun luo College of Safety Science and Engineering Liaoning Technical UniversityFuxin 123000China Key Laboratory of Mine Thermodynamic Disasters and Control(Ministry of Education) Fuxin 123000China

Injecting external CO_(2) into soft and low-permeability coal seams can improve CH4 extacctinn efficiency, and also benefit in CO_(2) sequestration. However, the distribution law of damage zone around borehole in soft coal seam and its effect on the efficiency of CO_(2) injection promoted CH4 extraction are not clear. In this paper, a multi-physics coupling mathematical model considering damage effect is established for simulating the process of CO_(2) injection promoted CH4 extraction in soft and low-permeability coal seam. The distribution of damage zone and permeability around boreholes under different diameters and coal strengths are analyzed. The gas pressure and gas content in coal seam during CO_(2) injection promoted CH4 extraction when the model considered damage effect are compared with that of ignored. The results show that small borehole diameter corresponds to narrow damage zone around the borehole in coal seam. The damage zone expands with the increase of the borehole diameter. The damage zone increases exponentially with the borehole diameter, while decreases exponentially with the compressive strength of coal seam. The highest permeability in the damage zone has increased by nearly 300 times under the condition of simulated case. CH4 pressure around the extraction borehole reduces, and the reduction area expands with the increase of time. Compared with the result of considering the damage effect, the reduction area of ignoring it is smaller, and the reducing speed is slower. The integrated effect of CO_(2) injection and CH4 extraction leads to rapid decrease of CH4 content in coal seam near the boreholes. The CO_(2) pressure and content increase around the injection borehole, and the increasing area gradually extends to the whole coal seam. In soft coal seams, failure to consider the damage effect will underestimate the efficiency of CH4 extraction and CO_(2) sequestration, resulting conservative layout of boreholes.

关键词： soft and low-permeability coal seam carbon dioxideinjection gas extraction damageeffect permeability evolution

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Elimination mechanism of coal and gas outburst based on geo‑dynamic system with stress–damage–seepage interactions

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International Journal of Coal Science & Technology 2023年第4期10卷 47-61页

作者： Lingjin Xu Chaojun Fan mingkun luo Sheng Li Jun Han Xiang Fu Bin Xiao College of Mining Liaoning Technical UniversityFuxin 123000China Energy Business Department Shanxi Lu’an Chemical GroupChangzhi 046200China Resource Department Liaoning Geology Engineering Vocational CollegeDandong 118000China

Coal and gas outburst is a complex dynamic disaster during coal underground mining.Revealing the disaster mechanism is of great signifcance for accurate prediction and prevention of coal and gas outburst.The geo-dynamic system of coal and gas outburst is proposed.The framework of geo-dynamic system is composed of gassy coal mass,geological dynamic environment and mining disturbance.Equations of stress–damage–seepage interaction for gassy coal mass is constructed to resolve the outburst elimination process by gas extraction with boreholes through layer in foor roadway.The results show the occurrence of outburst is divided into the evolution process of gestation,formation,development and termination of geo-dynamic system.The scale range of outburst occurrence is determined,which provides a spatial basis for the prevention and control of outburst.The formation criterion and instability criterion of coal and gas outburst are established.The formation criterion F1 is defned as the scale of the geo-dynamic system,and the instability criterion F2 is defned as the scale of the outburst geo-body.According to the geo-dynamic system,the elimination mechanism of coal and gas outburst—‘unloading+depressurization’is established,and the gas extraction by boreholes through layer in foor roadway for outburst elimination is given.For the research case,when the gas extraction is 120 days,the gas pressure of the coal seam is reduced to below 0.4 MPa,and the outburst danger is eliminated efectively.

关键词： Coal and gas outburst Geo-dynamic system Stress–damage–seepage coupling Elimination mechanism Instability criterion Gas extraction

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CRISPR-Cas9 sgRNA design and outcome assessment: Bioinformatics tools and aquaculture applications

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Aquaculture and Fisheries 2022年第2期7卷 121-130页

作者： mingkun luo Jun Wang Zaijie Dong Chenghui Wang Guoqing Lu Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Freshwater Fisheries Research Centre of Chinese Academy of Fishery SciencesMinistry of Agriculture and Rural AffairsWuxi214081China Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs/National Demonstration Center for Experimental Fisheries Science Education/Shanghai Engineering Research Center of AquacultureShanghai Ocean UniversityShanghai201306China Department of Biology University of Nebraska at OmahaOmahaNE68182USA

The CRISPR-Cas9 genome editing has many advantages over its counterparts and could ultimately assist in disease control and molecular breeding in aquaculture.Single-guide RNA(sgRNA)design is presumably the most crucial task in a typical CRISPR-Cas9 experiment;an understanding of algorithms behind sgRNA design programs is thus essential for improved efficacy and specificity.We focus this review on the bioinformatics aspects in genome editing experiments and describe commonly used computational approaches and tools of sgRNA design and outcome assessment.We show an example of sgRNA design with optimal parameter settings and appropriate interpretation of results and present a brief overview of CRISPR-Cas9 applications,such as genetic improvement and sustainability in aquaculture.We discuss challenging issues,particularly in the context of computational biology,in the use of computational tools in CRISPR-based genome editing.This review provides a synthesis of bioinformatics tools used for CRISPR-Cas9 sgRNA design and outcome assessment and offers a general view of CRISPR-based applications in farmed fish,which are expected to facilitate genome editing programs and hence improve aquaculture breeding,production,and sustainability.

关键词： CRISPR-Cas9 Genome editing sgRNA design Outcome assessment Bioinformatics tools Aquaculture applications

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Genomes of major fishes in world fisheries and aquaculture: Status, application and perspective

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Aquaculture and Fisheries 2020年第4期5卷 163-173页

作者： Guoqing Lu mingkun luo Department of Biology University of Nebraska at OmahaOmaha68182USA Wuxi Fisheries College Nanjing Agricultural UniversityJiangsuWuxi214081China

Capture fisheries and aquaculture provide a significant amount of high-quality protein to human beings and thus play an essential role in ending global hunger and malnutrition.The availability of tens of hundreds of fish genomes and the advances of genomics have allowed addressing many challenging issues such as overfishing and germplasm degradation faced by fisheries and aquaculture.In this review,we describe the current status of genomics in fisheries and aquaculture,with an emphasis on 14 species of fish that are considerably important to global fisheries and aquaculture,in the context of genome sequencing and assembly,annotation,GC contents,and repeats.The majority of these genomes are assembled at the chromosome level and annotated with proteins and pathways,with functional relevance to fisheries and aquaculture,such as environmental adaptation and phenotypic variation.We summarize potential genomic applications in fisheries and aquaculture that are related to assessment and use of genetic resources,disease resistance,growth and development,sexual determination,and fisheries management.Although much progress has been achieved in genomic application to fisheries and aquaculture,the full potential remains to be explored and reaped.We discuss the challenges and perspectives of genomics in translational aquaculture and fisheries,which include genome assembly and annotation,genomic selection and breeding,genomics in fisheries management,and integrated artificial intelligence systems.In the coming decades,we anticipate the applications of genomic techniques such as genome editing and genomic selection,along with the use of emerging intelligence systems,in aquaculture and fisheries will contribute significantly to genetic improvements of farmed fish and sustainable exploitation of fishery resources,which consequently lead to eradicating global poverty by 2030,an ambitious goal set by the United Nations.

关键词： Fish genomics World aquaculture Fisheries management Genomic selection Intelligent system

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鳙长江中下游群体的D-loop序列遗传分析

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上海海洋大学学报 2024年第3期33卷 521-532页

作者：傅建军朱文彬罗明坤王兰梅董在杰中国水产科学研究院淡水渔业研究中心、农业农村部淡水渔业与种质资源利用重点实验室江苏无锡214081

为了探究长江中下游鳙的种质遗传现状,该研究收集了石首(SS)、长沙(CS)、瑞昌(RC)、扬州(YZ)和张家港(ZJG)的长江种群(YR,219尾),对其D-loop序列进行检测分析,并结合NCBI下载整理的珠江种群(PR,213尾)和北美种群(NA,33尾)开展比较研究... 详细信息

为了探究长江中下游鳙的种质遗传现状,该研究收集了石首(SS)、长沙(CS)、瑞昌(RC)、扬州(YZ)和张家港(ZJG)的长江种群(YR,219尾),对其D-loop序列进行检测分析,并结合NCBI下载整理的珠江种群(PR,213尾)和北美种群(NA,33尾)开展比较研究。结果显示,在YR、PR和NA的D-loop序列中分别检测到35、96和11个变异位点,并分别界定37、62和3个单倍型。在长江中下游的5个鳙群体中,单倍型多样性(H_(d))为0.798~0.897,核苷酸多样性(π)为0.00338~0.00653。长江中下游5个鳙群体间,RC与ZJG(F_(ST)=0.012)和CS与YZ(F_(ST)=0.018)的遗传分化不显著,其余群体间均存在显著遗传分化(F_(ST)为0.031~0.125);群体间检测到不同程度的基因流(N_(m)为3.509~39.993)。基于单倍型网络分析,长江中下游的鳙D-loop序列单倍型存在多个分支,并在群体间存在明显的共享;YR和PR的中心单倍型存在广泛共享,PR的特有单倍型均为外围单倍型,并推测NA的形成存在不同的遗传来源。基于中性检验结果,推测PR在历史上经历过种群扩张。研究表明,长江和珠江的鳙种群维持了较高的遗传多样性,可为其种质资源保护和利用奠定物质基础。

关键词：鳙线粒体DNA D-loop序列种群多样性遗传分化

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不同体色福瑞鲤2号的肌肉品质

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水产学报 2024年第4期48卷 289-297页

作者：龚雅婷王兰梅朱文彬傅建军罗明坤董在杰南京农业大学无锡渔业学院中国水产科学研究院淡水渔业研究中心农业农村部淡水渔业与种质资源利用重点实验室

为探究鲤不同体色个体肌肉品质的差异，本研究以福瑞鲤2号青灰色个体(BG)和全红个体(WR)为对象，通过检测其肌肉色泽、氨基酸、脂肪酸和质构指标，评价这2种体色福瑞鲤2号肌肉品质的差异。结果显示，BG的b*值显著高于WR；出肉率以及肌... 详细信息

为探究鲤不同体色个体肌肉品质的差异，本研究以福瑞鲤2号青灰色个体(BG)和全红个体(WR)为对象，通过检测其肌肉色泽、氨基酸、脂肪酸和质构指标，评价这2种体色福瑞鲤2号肌肉品质的差异。结果显示，BG的b*值显著高于WR；出肉率以及肌肉的水分、粗脂肪、粗蛋白和灰分无显著差异；不同体色福瑞鲤2号的肌肉中均能检测出17种氨基酸，其中人体必需氨基酸7种，非必需氨基酸10种。甘氨酸(Gly)、脯氨酸(Pro)、组氨酸(His)和半胱氨酸(Cys)在2组间差异不显著，其他氨基酸在2组间存在显著或极显著差异，两种体色福瑞鲤2号之间ΣEAA/ΣTAA没有显著差异；BG肉豆蔻酸(C14:0)显著高于WR,BG的饱和脂肪酸含量较WR稍高，WR的单不饱和脂肪酸含量较BG稍高，二者多不饱和脂肪酸含量相近；WR的弹性和咀嚼性显著高于BG。研究表明，体色对福瑞鲤2号的肌肉品质有影响。本研究为鱼类肉质性状改良提供基础数据和参照依据。

关键词：福瑞鲤2号色泽氨基酸脂肪酸质构

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我国苏南地区美洲鲥7个养殖群体的遗传多样性分析

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上海海洋大学学报 2024年第2期33卷 285-296页

作者：李玉林罗明坤冯冰冰朱文彬傅建军梁政远解旭东缪凌鸿董在杰南京农业大学无锡渔业学院江苏无锡214081 中国水产科学研究院淡水渔业研究中心、农业农村部淡水渔业与种质资源利用重点实验室江苏无锡214081 江苏省渔业技术推广中心江苏南京210036 无锡瑞顺水产养殖科技有限公司江苏无锡214001 镇江欣润农业发展有限公司江苏镇江212115

为探讨苏南地区美洲鲥养殖群体的种质资源现状及遗传多样性水平,采用15个微卫星标记和线粒体D-loop序列,对7个不同群体:镇江丹徒(Dtq)、镇江扬中(Yzq)、苏州张家港(Zjg)、苏州相城(Xcq)、南通中洋(Zyq)、常州滆湖(Ghq)和常州武进(Czq)共... 详细信息

为探讨苏南地区美洲鲥养殖群体的种质资源现状及遗传多样性水平,采用15个微卫星标记和线粒体D-loop序列,对7个不同群体:镇江丹徒(Dtq)、镇江扬中(Yzq)、苏州张家港(Zjg)、苏州相城(Xcq)、南通中洋(Zyq)、常州滆湖(Ghq)和常州武进(Czq)共计210尾个体进行群体多样性分析。结果显示,15个SSR位点中除Asa-12外,其余位点均表现为高度多态性(PIC>0.5)。其中,7个群体期望杂合度(He)为0.615~0.758,多态信息含量(PIC)为0.568~0.723,两者均以Zjg群体最高。D-loop序列共检测到32个变异位点,定义了20个单倍型,其中Zjg群体单倍型最多(11个)。7个群体的单倍型多样性、核苷酸多样性指数分别为0.618~0.945、0.003~0.008。基于SSR和D-loop序列的遗传距离分析,发现Ghq群体和Zyq群体的Nei's遗传距离(0.058)和K2P遗传距离(0.003)最近,低于其他群体间的遗传距离(分别为0.073~0.397和0.003~0.006)。综合分析,发现7个美洲鲥群体的遗传多样性较为丰富,而群体间遗传变异程度不高,基因流N_(m)>1和镶嵌式排列的个体进化树也证实7个群体的亲缘关系较近。基于本研究,可初步了解苏南地区鲥的种质现状,为后续进一步开展育种工作奠定理论基础。

关键词：美洲鲥微卫星 D-loop区遗传多样性

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套养池塘主要养殖阶段浮游生物的群落结构特征

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水产学杂志 2024年第1期37卷 104-112页

作者：傅建军安睿朱文彬王兰梅罗明坤董在杰中国水产科学研究院淡水渔业研究中心农业农村部淡水渔业和种质资源利用重点实验室江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214128

为探究长江中下游地区套养池塘主要养殖阶段浮游生物群落结构特征及其影响因素,于2019年5—9月,在中国水产科学研究院淡水渔业研究中心宜兴屺亭科研实验基地主养鲤(Cyprinus carpio,“福瑞鲤2号”)和尼罗罗非鱼(Oreochromis niloticus,... 详细信息

为探究长江中下游地区套养池塘主要养殖阶段浮游生物群落结构特征及其影响因素,于2019年5—9月,在中国水产科学研究院淡水渔业研究中心宜兴屺亭科研实验基地主养鲤(Cyprinus carpio,“福瑞鲤2号”)和尼罗罗非鱼(Oreochromis niloticus,“中威1号”)、套养鲢(Hypophthalmichthys molitrix)和鳙(H.nobilis),采用人工投喂的常规鱼种培育池塘中,每间隔15~25 d测定水体理化指标及浮游生物种类和数量。结果显示,主要养殖阶段池塘总氮和氨氮浓度呈上升趋势,并逐渐趋于稳定;亚硝酸氮含量在养殖周期内浓度均较低,总磷先上升、后下降。实验中共检测到浮游植物8门77属,以绿藻门和蓝藻门为主;浮游动物5类72种,以原生动物和轮虫类为主。基于浮游生物密度的主成分分析(PCA)和热图显示,实验期间浮游生物群落结构具有明显的时序性变化,优势种及其密度变化明显。冗余分析(RDA)表明,总氮和亚硝酸氮浓度显著影响浮游植物丰度(P<0.05);水温、p H和亚硝酸氮浓度显著影响浮游动物丰度(P<0.05)。14个浮游生物优势种的密度与水体理化指标间存在显著线性相关(P<0.05)。结果表明,长江中下游地区套养池塘主要养殖阶段浮游生物群落结构时序性变化明显,水体理化指标存在差异。本研究结果可为套养模式下池塘的水质监测和调控及养殖管理提供参考数据。

关键词：浮游生物群落结构环境因子 RDA分析

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Coal and gas outburst dynamic system

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International Journal of Mining Science and Technology 2017年第1期27卷 49-55页

作者： Fan Chaojun Li Sheng luo mingkun Du Wenzhang Yang Zhenhua College of Mining Engineering Liaoning Technical UniversityFuxin 123000China State Key Laboratory for Geo Mechanics and Deep Underground Engineering China University of Mining&Technology Xuzhou 221116China State Key Laboratory of Coal Resources and Safe Mining China University of Mining&TechnologyXuzhou 221116China

Coal and gas outburst is an extremely complex dynamic disaster in coal mine production process which will damage casualties and equipment facilities, and disorder the ventilation system by suddenly ejecting a great amount of coal and gas into roadway or working face. This paper analyzed the interaction among the three essential elements of coal and gas outburst dynamic system. A stress-seepage-damage coupling model was established which can be used to simulate the evolution of the dynamical system, and then the size scale of coal and gas outburst dynamical system was investigated. Results show that the dynamical system is consisted of three essential elements, coal-gas medium(material basis), geology dynamic environment(internal motivation) and mining disturbance(external motivation). On the case of C13 coal seam in Panyi Mine, the dynamical system exists in the range of 8–12 m in front of advancing face. The size scale will be larger where there are large geologic structures. This research plays an important guiding role for developing measures of coal and gas outburst prediction and prevention.

关键词：煤与瓦斯突出预测通风系统动力系统地质构造生产过程设备设施人员伤亡相互作用

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Structure and deformation measurements of shallow overburden during top coal caving longwall mining

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International Journal of Mining Science and Technology 2017年第6期27卷 1081-1085页

作者： Li Sheng Fan Chaojun luo mingkun Yang Zhenhua Lan Tianwei Zhang Haifeng College of Mining Liaoning Technical University State and Local Joint Engineering Laboratory for Gas Drainage & Ground Control of Deep Mines Henan Polytechnic University State Key Laboratory of Coal Resources and Safe Mining China University of Mining & Technology Chengyi Coal Mine Inner Mongolia Yitai Coal Co.Ltd.

Mining induced pressures are strong and overburden failure areas are large in top coal caving longwall mining, which constrains high production and safety mining. By employing the combination of the full view borehole photography technique and the seismic CT scanner technique, the deformation and failure of overlying strata of fully mechanized caving face in shallow coal seam were studied and the failure development of overburden was determined. Results show that the full view borehole photography can reveal the characteristics of strata, and the seismic CT scanner can reflect the characteristics of strata between the boreholes. The combined measurement technique can effectively determine the height of fractured and caved zones. The top end of the caved zone in Yangwangou coal mine employing the top coal caving longwall mining was at the depth of 171 m and fractured zone was at the depth of 106-110 m. The results provide a theoretic foundation for controlling the overburden strata in the shallow buried top coal caving panel.

关键词： Top coal caving Shallow depth Overburden failure and deformation Full view borehole photography Seismic CT scanner

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