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Cell membrane sample preparation method of combined AFM and dSTORM analysis

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Biophysics Reports 2022年第4期8卷 183-192页

作者： mingjun cai Huili Wang Guanfang Zhao Hongru Li Jing Gao Hongda Wang University of Science and Technology of China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology (Qingdao)

A major role of cell membranes is to provide an ideal environment for the constituent proteins to perform their biological functions. A deep understanding of the membrane proteins assembly process under physiological conditions is quite important to elucidate both the structure and the function of the cell membranes. Along these lines, in this work, a complete workflow of the cell membrane sample preparation and the correlated AFM and dSTORM imaging analysis methods are presented. A specially designed, angle-controlled sample preparation device was used to prepare the cell membrane *** correlated distributions of the specific membrane proteins with the topography of the cytoplasmic side of the cell membranes can be obtained by performing correlative AFM and dSTORM measurements. These methods are ideal for systematically studying the structure of the cell membranes. The proposed method of the sample characterization was not only limited to the measurement of the cell membrane but also can be applied for both biological tissue section analysis and detection.

关键词： Atomic force microscopy (AFM) Super-resolution microscopy (SRM) Direct stochastic optical reconstruction microscopy (dSTORM) Cell membrane Combined technique

Mechanistic Insights into Membrane Protein Clustering Revealed by Visualizing EGFR Secretion

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Research 2023年第1期2022卷 353-366页

作者： Haijiao Xu Jinrui Zhang Yijia Zhou Guanfang Zhao mingjun cai Jing Gao Lina Shao Yan Shi Hongru Li Hongbin Ji Yikai Zhao Hongda Wang State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022 JilinChina State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 200031China University of Science and Technology of China Hefei230026 AnhuiChina University of Chinese Academy of Sciences Beijing 100049China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 130102China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266237 ShandongChina

Most plasmalemmal proteins are organized into clusters to modulate various cellular ***,the machineries that regulate protein clustering remain largely ***,with EGFR as an example,we directly and in detail visualized the entire process of EGFR from synthesis to secretion onto the plasma membrane(PM)using a high-speed,high-resolution spinning-disk confocal ***,colocalization imaging revealed that EGFR secretory vesicles underwent transport from the ER to the Golgi to the PM,eventually forming different distribution forms on the apical and basal membranes;that is,most EGFR formed larger clusters on the apical membrane than the basal membrane.A dynamic tracking image and further siRNA interference experiment confirmed that fusion of secretory vesicles with the plasma membrane led to EGFR clusters,and we showed that EGFR PM clustering may be intimately related to EGFR signaling and cell ***,we found that the size and origin of the secretory vesicles themselves may determine the difference in the distribution patterns of EGFR on the *** importantly,we showed that actin influenced the EGFR distribution by controlling the fusion of secretory vesicles with the ***,a comprehensive understanding of the EGFR secretion process helps us to unravel the EGFR clustering process and elucidate the key factors determining the differences in the spatial distribution of EGFR PM,highlighting the correlation between EGFR secretion and its PM distribution pattern.

关键词： distribution EGFR eventually

Evaluating the efficacy of the anticancer drug cetuximab by AFM

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Evaluating the efficacy of the anticancer drug cetuximab by ...

我国基于技术接受模型的移动图书馆用户感知行为研究综述

第十二届中国酶工程学术研讨会

作者： mingjun cai Hongda Wang Changchun Institute of Applied Chemistry State Key Laboratory of Electroanalytical Chemistry Chinese Academy of Science

Epidermal growth factor receptor（EGFR）, which belongs to the family of transmembrane receptor tyrosine kinases, and its ligand epidermal growth factor（EGF） are found in the majority of eukaryote cells and play important roles in cellular growth, survival, adhesion, migration, and differentiation. Cetuximab is a monoclonal antibody that binds to the epidermal growth factor receptor, which is important in the growth of many cancers. We adopted atomic force microscopy（AFM） to measure mechanical properties of cancer cells following cetuximab treatment and biomechanical properties cetuximab and epidermal growth factor receptor interactions. The stiffness of cancer cells following treatment with cetuximab was significantly higher than that of normal cells under the same conditions. Nanomechanical analysis provides a robust method for measuring the effects of anticancer drugs on living cells under physiological conditions. In addition, the results of SMFS indicate that the binding ability of cetuximab to EGFR in single cells is stronger than that of EGF to EGFR. These results indicate that AFM can effectively evaluate the efficacy of cetuximab with respect to its mechanical and biomechanical properties under physiological conditions.

关键词： AFM SMFS Young’s modulus cellular stiffness EGFR Cetuximab

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图书馆论坛 2017年第7期37卷 125-131页

作者：明均仁郭财强张俊武汉工程大学管理学院

近年我国学者关注移动图书馆用户的感知行为问题,并将技术接受模型(TAM)作为研究的理论基础,除考虑感知有用性、感知易用性及行为意向等传统TAM变量因素外,还引入其他外部变量和内部变量,对用户感知行为进行不同程度解释和验证。为整体... 详细信息

近年我国学者关注移动图书馆用户的感知行为问题,并将技术接受模型(TAM)作为研究的理论基础,除考虑感知有用性、感知易用性及行为意向等传统TAM变量因素外,还引入其他外部变量和内部变量,对用户感知行为进行不同程度解释和验证。为整体了解我国移动图书馆用户感知行为研究进展,文章统计我国TAM研究文献,重点对移动图书馆TAM模型构建及扩展路径、潜变量、实证研究等方面进行归纳与讨论,对后续有关移动图书馆用户感知行为研究提出建议,以进一步动态预测与解释用户行为,提高图书馆移动服务质量。

关键词：移动图书馆用户感知行为技术接受模型移动服务质量综述

Real-time Tracking Trans-membrane Transport of Gold Nanocages by Force Tracing Based on Atomic Force Microscopy

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Real-time Tracking Trans-membrane Transport of Gold Nanocage...

Evaluation of Nano-drug Efficacy by Nanoindentation Based on Atomic Force Spectroscopy

第十一届全国化学生物学学术会议

作者： Yu Yang Qingrong Zhang mingjun cai Haijiao Xu Denghua Lu Yanfeng Fu Yuping Shan School of Chemistry and Life Science Advanced Institute of Materials ScienceChangchun University of Technology

Currently,a popular field in nanomedicine is the implementation of gold nanocages(Au NCs) for cancer diagnosis and photothermal therapy .How gold nanocages trans-membrane is a fundamental issue for their use in photothermal *** trans-membrane of Au NCs is a dynamic process,the kinetic parameters and mechanism of Au NCs transporting still *** molecule force spectroscopy-based force tracking technology has been proven to be an excellent method for studying the dynamics of nanoparticles trans-membrane .We report the force tracing technique for tracking two sizes of Au NCs trans-membrane in real-time and obtain a series of dynamic parameters to compare,such as differences on force,duration,displacement,and *** study will provide a theoretical basis for screening the optimal Au NCs in photothermal therapy and has great promise for synergistic cancer treatment.

关键词： Au NCs Forcing tracing dynamic trans-membrane transporting AFM

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Evaluation of Nano-drug Efficacy by Nanoindentation Based on...

第十一届全国化学生物学学术会议

作者： Yanfeng Fu Yu Yang Qingrong Zhang mingjun cai Haijiao Xu Denghua Lu Yuping Shan School of Chemistry and Life Science Advanced Institute of Materials ScienceChangchun University of Technology

The nano-drug with the dual advantages of targeted drug delivery and controlled drug release has become a research hotspot for new antitumor drugs due to its unique *** needs to be evaluated in terms of transmission speed,cytotoxicity,and duration of *** evaluation methods are numerous and *** order to solve this problem,we tried to find a way to evaluate nano-drug *** this report,nanoindentation based on atomic force microscopy(AFM) was used to evaluate the effects of nano-drugs on living *** difference of drug efficacy for liposomes encapsulated doxorubicin(DOX) and camptothecin(CPT) at different conditions was *** results show that nanoindentation based on atomic force microscopy(AFM) could be a potential technique for evaluation nano-drug efficacy by detection the mechanical properties of the nano-drugs treated cells.

关键词： Nano-drug efficacy nanoindentation AFM

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腾冲火山地区微震观测(Ⅰ)

地震研究 1998年第4期 53-58页

作者： Ye Jianqing Chen Mingong Liu Xuejun cai Shaoping cai mingjun Qin Jiazheng Huangfu Gang Liu Zuyin (Seismological Bureau of Yunnan Province, Kunming, 650041)

本文综述了腾冲火山地区微震观测台网的建立与所取得的具有研究价值的地震记录。内容包括观测系统性能、台站布局、地震记录。由观测资料表明腾冲火山地区存在微震活动。讨论了台网需要努力的目标。

关键词：微震观测腾冲火山地区

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礼法之规度——张璁《正典礼第一》疏义

温州大学学报（社会科学版） 2013年第3期26卷 57-61页

作者：蔡明均贵州大学人文学院贵州贵阳550025

明世宗朱厚熜登位不久,因在"大礼议"中与杨廷和各执一端,张璁上疏《正典礼第一》支持朱厚熜,驳斥杨廷和之论。张璁认为杨廷和要求朱厚熜继嗣其伯父明孝宗朱祐樘的做法,违背了天道人伦。因为兴献王朱祐祐与朱厚熜之间才是天然的父子关系... 详细信息

明世宗朱厚熜登位不久,因在"大礼议"中与杨廷和各执一端,张璁上疏《正典礼第一》支持朱厚熜,驳斥杨廷和之论。张璁认为杨廷和要求朱厚熜继嗣其伯父明孝宗朱祐樘的做法,违背了天道人伦。因为兴献王朱祐祐与朱厚熜之间才是天然的父子关系,所以朱厚熜不能继嗣朱佑樘,而朱厚熜身为朱祐樘之子明武宗朱厚照的亲堂弟,则可以继承其皇位。张璁以经论礼,指出儒家的《礼记》及明太祖朱元璋的《皇明祖训》两种经典对礼法的规定,才是判别朱厚熜应该继统还是继嗣的根本依据。对张璁这份奏疏的义理进行解读,可以看出礼法对明世宗初政的规范和制约。

关键词：张璁《正典礼第一》大礼议

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来华留学生辅导员队伍建设研究综述

科教导刊 2021年第19期 177-179页

作者：蔡明均电子科技大学国际教育学院四川成都611731

加强来华留学生辅导员队伍建设是坚持社会主义办学方向、构建教育对外开放新格局、提升来华留学生教育质量的客观需要和制度保障。把握当下来华留学生辅导员队伍建设的研究现状和主要成果,能够为今后深化来华留学生辅导员队伍建设的理... 详细信息

加强来华留学生辅导员队伍建设是坚持社会主义办学方向、构建教育对外开放新格局、提升来华留学生教育质量的客观需要和制度保障。把握当下来华留学生辅导员队伍建设的研究现状和主要成果,能够为今后深化来华留学生辅导员队伍建设的理论研究和实践探索提供参考借鉴。通过文献梳理可知,理论界和教育界围绕设立留学生辅导员的必要性,留学生辅导员的角色定位、价值作用和职责要求,留学生辅导员队伍建设的现状和举措开展了相关研究。后疫情时代通过加强留学生辅导员队伍建设的学术研究、深化留学生辅导员队伍建设的实践创新,才能切实有效地推动留学生辅导员的队伍建设。

关键词：来华留学生辅导员队伍建设研究综述