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石见穿多糖对结肠癌SW620细胞上皮间质转化的影响

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世界科学技术-中医药现代化 2024年第2期26卷 311-319页

作者：摆丽蒋明鸿吴润霞王其美罗吉湖南中医药大学中西医结合学院长沙410208 湖南省中医药研究院附属医院长沙410006

目的研究石见穿多糖对结肠癌SW620细胞上皮间质转化(Epithelial-mesenchymal transition,EMT)的影响,探讨石见穿多糖抑制结肠癌侵袭转移的作用机制。方法采用不同浓度石见穿多糖对SW620细胞进行干预,干预时间分别为12小时和24小时;CCK-... 详细信息

目的研究石见穿多糖对结肠癌SW620细胞上皮间质转化(Epithelial-mesenchymal transition,EMT)的影响,探讨石见穿多糖抑制结肠癌侵袭转移的作用机制。方法采用不同浓度石见穿多糖对SW620细胞进行干预,干预时间分别为12小时和24小时;CCK-8法检测各组细胞的增殖,并选择后续实验的药物浓度和时间;细胞划痕和Transwell迁移实验来检测各组细胞的迁移能力;Transwell侵袭实验检测各组细胞的侵袭力;Western blot和qRT-PCR方法检测EMT相关蛋白E-cadherin、Vimentin及其mRNA,以及转录因子ZEB1、ZEB2、Twist1及其mRNA的表达。结果与对照组比较,石见穿多糖能够抑制SW620细胞的增殖,且浓度越高、干预时间越长,抑制作用越强,且呈浓度和时间依赖性。因此,在后续实验中,选择0.25 mg·mL^(-1)、1 mg·mL^(-1)、2 mg·mL^(-1)、3 mg·mL^(-1)作为干预浓度,选择24 h作为干预时间。与对照组比较,石见穿多糖能够抑制SW620细胞的迁移和侵袭,且浓度越高,抑制作用越强,呈浓度依赖性;与对照组比较,石见穿多糖能够上调E-cadherin及其mRNA的表达,且浓度越高,表达水平越高,呈浓度依赖性;同时下调Vimentin、ZEB1、ZEB2、Twist1及其mRNA的表达,且浓度越高,表达水平越低,呈浓度依赖性。结论石见穿多糖能够抑制SW620细胞的增殖、迁移和侵袭,其机制可能与上调E-cadherin的表达,下调Vimentin、ZEB1、ZEB2和Twist1的表达,从而抑制EMT过程有关。

关键词：石见穿多糖 SW620细胞增殖迁移与侵袭上皮间质转化

E3 ubiquitin ligase RNF170 inhibits innate immune responses by targeting and degrading TLR3 in murine cells

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Cellular & Molecular Immunology 2020年第8期17卷 865-874页

作者： Xiaoqi Song Shuo Liu Wendie Wang Zhongfei Ma Xuetao Cao minghong jiang National Key Laboratory of Medical Molecular Biology Department of Immunology&Center for ImmunotherapyInstitute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical College100005BeijingChina National Key Laboratory of Medical Immunology&Institute of Immunology Second Military Medical University200433ShanghaiChina School of Medicine Nankai University300071TianjinChina

Upon recognition of dsRNA,toll-like receptor 3(TLR3)recruits the adaptor protein TRIF to activate IRF3 and NF-κB signaling,initiating innate immune *** ubiquitination of TLR3 downstream signaling molecules and their roles in the innate response have been discovered;however,whether TLR3 itself is ubiquitinated and then functionally involved remains to be *** immunoprecipitating TLR3-binding proteins in macrophages,we identified ring finger protein 170(RNF170)as a TLR3-binding E3 ***170 mediated the K48-linked polyubiquitination of K766 in the TIR domain of TLR3 and promoted the degradation of TLR3 through the proteasome *** genetic ablation of RNF170 selectively augmented TLR3-triggered innate immune responses both in vitro and in *** results reveal a novel role for RNF170 in selectively inhibiting TLR3-triggered innate immune responses by promoting TLR3 degradation.

关键词： TLR3 RNF170 Ubiquitination Degradation Innate immunity

Oxidized galectin-1 in SLE fails to bind the inhibitory receptor VSTM1 and increases reactive oxygen species levels in neutrophils

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Cellular & Molecular Immunology 2023年第11期20卷 1339-1351页

作者： Xu jiang Xinyue Xiao Hao Li Yiyi Gong Min Wang Huaxia Yang Lidan Zhao Ying jiang Yanping Wei Chongchong Zhao Jin Li Yuling Chen Shan Feng Haiteng Deng Shiliang Ma Yue Xu Yudong Liu George C.Tsokos minghong jiang Xuan Zhan Medical Research Center State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalDepartment of RheumatologyBeijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijingChina Department of Rheumatology Key Laboratory of MyositisChina-Japan Friendship HospitalBeijingChina Department of Medicine Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMAUSA Medical Research Center Peking Union Medical College HospitalChinese Academy of Medical SciencesBeijingChina Department of Rheumatology Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineClinical Immunology CenterChinese Academy of Medical SciencesBeijingChina Department of Rheumatology and Clinical Immunology Peking Union Medical College HospitalClinical Immunology CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeThe Ministry of Education Key LaboratoryBeijingChina Department of Rheumatology Xiangya HospitalCentral South UniversityHunanChina MOE Key Laboratory of Bioinformatics Center for Synthetic&Systems BiologySchool of Life SciencesTsinghua UniversityBeijingChina Key Laboratory of Structural Biology of Zhejiang Province School of Life SciencesWestlake UniversityHangzhouZhejiangChina State Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijingChina

Inhibitory immune receptors set thresholds for immune cell activation,and their deficiency predisposes a person to autoimmune ***,the agonists of inhibitory immune receptors remain largely unknown,representing untapped sources of treatments for autoimmune ***,we show that V-set and transmembrane domain-containing 1(VSTM1)is an inhibitory receptor and that its binding by the competent ligand soluble galectin-1(Gal1)is essential for maintaining neutrophil viability mediated by downregulated reactive oxygen species ***,in patients with systemic lupus erythematosus(SLE),circulating Gal1 is oxidized and cannot be recognized by VSTM1,leading to increased intracellular reactive oxygen species levels and reduced neutrophil *** neutrophil function or death contributes significantly to the pathogenesis of SLE by providing danger molecules and autoantigens that drive the production of inflammatory cytokines and the activation of autoreactive ***,serum levels of glutathione,an antioxidant able to convert oxidized Gal1 to its reduced form,were negatively correlated with SLE disease *** together,our findings reveal failed inhibitory Gal1/VSTM1 pathway activation in patients with SLE and provide important insights for the development of effective targeted therapies.

关键词： Systemic lupus erythematosus ROS VSTM1 Galectin-1 Glutathione

8-25浮吊转台转柱连接螺栓断裂故障分析及处理

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港口装卸 1999年第1期 16-17页

作者：姜明鸿武汉交通科技大学武汉430063

1 前言某发电厂一台8-25浮吊频繁发生转台、转柱法兰连接螺栓断裂等故障.大修后,将原4m3抓斗改为3m3,并调整了其死配重.但使用不到一个月,又出现此故障.

关键词： 8-25 浮吊转台螺栓断裂故障转柱连接螺栓

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清代苏南家族的历史教学法刍议

历史教学问题 2009年第2期 14-17页

作者：蒋明宏江南大学教育学院

在清代尤其晚近时期，苏南家族的历史教育顺应时代、积极变革，显得十分活跃，在教学方法上也表现出丰富多彩、不拘传统的特点。文章对清代苏南家族历史教育中的激趣诱导、明体为上、讨论商榷、赞赏讽喻、知行结合等教学方法作了探讨，... 详细信息

在清代尤其晚近时期，苏南家族的历史教育顺应时代、积极变革，显得十分活跃，在教学方法上也表现出丰富多彩、不拘传统的特点。文章对清代苏南家族历史教育中的激趣诱导、明体为上、讨论商榷、赞赏讽喻、知行结合等教学方法作了探讨，并分析了教学方法变革的原因和内涵。

关键词：清代苏南家族历史教育教学方法

The role of MTTP in the progress of HBV infection

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The role of MTTP in the progress of HBV infection

创业、变革与创新趣事--1931-1937:唐程丽新纺织集团的黄金时代

第十三届全国免疫学学术大会

作者： Zhongfei Ma minghong jiang Shuo Liu Cheng Wang Xuetao Cao Institute of Basic Medical Sciences Chinese Academy of Medical Sciences

MTTP(Microsomal Triglyceride Transfer Protein) is the large subunit of a heterodimeric complex which is highly expresses in *** is an essential intracellular lipid transfer protein that catalyzes the transport of triglyceride, cholesteryl ester and phospholipid molecules passing through between *** has shown that the genotype distribution of MTTP-493 G/T demonstrated a significant difference between the self-limited infection group and the chronic *** MTTP is also inevitable for the hepatocytes’ antigen-presenting responsible for NKT cell *** hypothesized that the MTTP plays a role in the progress of HBV *** the experiment we found that when inhibiting the function of MTTP in HepG2.2.15 cell, the HBsAg secretion and pgRNA expression increased *** preliminary findings recommended that the MTTP played a role in the progress of HBV infection other than just involving in antigen presenting.

关键词： HBV MTTP antiviral immunity

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档案与建设 2018年第12期1卷 49-51页

作者：蒋明宏江南大学人文学院江苏无锡214025

一、吃斋女子的创业如果追溯唐程纺织集团的创业缘起,那要说到上个世纪初无锡东门附近映山河一个庵堂里,由一些吃斋女子所创办的手工织布的“冠华手工织布厂”。其中荣茹是她们的带头人。

关键词：纺织集团变革与创新黄金时代创业趣事织布厂带头人女子

基于拓展的倍增法的无穷限广义积分数值计算分析与研究

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科技通报 2012年第12期28卷 9-11页

作者：姜明红呼伦贝尔学院内蒙古呼伦贝尔021000

为了解决复杂函数的无穷限广义积分无法通过分步积分法得到精确结果的问题,科研工作者提出了以数值逼近的方法去求解该类积分的近似数值解,用该数值解去替代原广义积分。在实际应用中发现,此方法存在潜在的悖论问题。针对该问题,提出将... 详细信息

为了解决复杂函数的无穷限广义积分无法通过分步积分法得到精确结果的问题,科研工作者提出了以数值逼近的方法去求解该类积分的近似数值解,用该数值解去替代原广义积分。在实际应用中发现,此方法存在潜在的悖论问题。针对该问题,提出将倍增法应用到无穷限广义积分的数值求解中。通过将倍增法进行有针对性地拓展,从而既解决了潜在的悖论问题,又提升了数值计算速度与稳定性。从而为无穷限广义积分在计算数学、应用数学、经济学等学科中的更进一步推广打下了坚实的基础。

关键词：拓展倍增无穷限广义积分数值计算

实学的力量:化愚顽为英才——晚近苏南民间教育智慧研究

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南京社会科学 2013年第5期 137-142页

作者：于书娟蒋明宏江南大学田家炳教育学院

本文选取晚近以来苏南各个领域中的若干巨擘、英才,如钱承沛与钱穆、华翼纶与华蘅芳父子,钱基博与钱基厚、荣德生与荣宗敬兄弟等为研究对象,通过对他们被发现、培育及成长历程的考察,总结苏南地区人才成长的历史经验,探索民间的教育智慧... 详细信息

本文选取晚近以来苏南各个领域中的若干巨擘、英才,如钱承沛与钱穆、华翼纶与华蘅芳父子,钱基博与钱基厚、荣德生与荣宗敬兄弟等为研究对象,通过对他们被发现、培育及成长历程的考察,总结苏南地区人才成长的历史经验,探索民间的教育智慧,以期为当下的教育变革、教育公平提供一种历史的启示。

关键词：晚近苏南民间教育实学教育智慧