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Allosteric DNAzyme-based encoder for molecular information transfer

Chinese Chemical Letters 2024年第7期35卷 235-241页

作者： minghao Hu Tianci Xie Yuqiang Hu Longjie Li Ting Wang Tongbo Wu School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Oncology Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China GI Cancer Research Institute Tongji HospitalHuazhong University of Science and TechnologyWuhan 430030China School of Life Science and Technology Wuhan Polytechnic UniversityWuhan 430023China State Key Laboratory of Bioelectronics National Demonstration Center for Experimental Biomedical Engineering EducationSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

Dynamic DNA nanotechnology plays a significant role in nanomedicine and information science due to its high programmability based on Watson-Crick base pairing and nanoscale *** DNA machines and networks have been widely used in various fields,including molecular imaging,biosensors,drug delivery,information processing,and logic *** serve as crucial components for information compilation and transfer,allowing the conversion of information from diverse application scenarios into a format recognized and applied by DNA ***,there are only a few encoder designs with DNA ***,the molecular priority encoder is hardly *** this study,we introduce allosteric DNAzyme-based encoders for information *** design of the allosteric domain and the recognition arm allows the input and output to be independent of each other and freely *** pre-packaged mode design achieves uniformity of baseline dynamics and dynamics *** also integrated non-nucleic acid molecules into the encoder through the aptamer design of the allosteric ***,we developed the 2^(n)-n encoder and the EndoⅣ-assisted priority encoder inspired by immunoglobulin's molecular structure and effector *** our knowledge,the proposed encoder is the first enzyme-free DNA encoder with DNA output,and the priority encoder is the first molecular priority encoder in the DNA reaction *** encoders avoid complex operations on a single molecule,and their simple structure facilitates their application in complex DNA circuits and biological scenarios.

关键词： DNAzyme Encoder Nucleic acids DNA circuit DNA strand displacement Dynamic DNA nanotechnology

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Quantitative liquid storage by billiards-like droplet collision on surfaces with patterned wettability

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Droplet 2024年第3期3卷 80-90页

作者： minghao Li Haoxu Yu Zhirui Liu Ziyue Gao Faze Chen School of Mechanical Engineering Tianjin UniversityTianjinChina Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education Tianjin UniversityTianjinChina

There has been significant interest in researching droplet transport behavior on com-posite wetting ***,current research is primarily focused on modifying individual droplets and lacks an in-depth investigation into high-precision droplet *** study introduces a“billiard ball”droplet transport and storage platform(TSP)with differentiated *** this platform,the volume of droplets stored in the area reaches a consistent threshold through droplet“scrambling,”inspired by the water-gathering behavior of *** TSP involves connecting two regions of different sizes using a three-dimensional stepped wedge angle ***-ever,this connection is not seamless,leaving a 2-mm gap between the *** gap is intentionally designed to enable continuous droplet transfer while preventing any static *** systematic experimental and simulation analysis,we investigated the influence of superhydrophilic pattern structures and parameters on quantitative droplet *** established a functional relationship between the pattern area and the stored volume,and analyzed the intrinsic mechanism of droplet collision *** enabled us to achieve on-demand quantitative droplet stor-age and autonomize the storage ***“billiard ball”droplet transport–storage platform proposed in this study holds promising applications in the fields of biomedical and organic chemistry.

关键词： structure. process. collision

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Cross-Modal Graph Semantic Communication Assisted by Generative AI in the Metaverse for 6G

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Research 2024年第2期2024卷 19-27页

作者： Mingkai Chen minghao Liu Congyan Wang Xingnuo Song Zhe Zhang Yannan Xie Lei Wang Key Laboratory of Broadband Wireless Communication and Sensor Network Technology Nanjing University of Posts and TelecommunicationsNanjng 210003China State Key Laboratory of Organic Electronics and Information Displays&Institute of Advanced Materials(IAM) Nanjing University of Posts and TelecommunicationsNanjing 210023China

Recently, the development of the Metaverse has become a frontier spotlight, which is an important demonstration of the integration innovation of advanced technologies in the Internet. Moreover, artificial intelligence (AI) and 6G communications will be widely used in our daily lives. However, the effective interactions with the representations of multimodal data among users via 6G communications is the main challenge in the Metaverse. In this work, we introduce an intelligent cross-modal graph semantic communication approach based on generative AI and 3-dimensional (3D) point clouds to improve the diversity of multimodal representations in the Metaverse. Using a graph neural network, multimodal data can be recorded by key semantic features related to the real scenarios. Then, we compress the semantic features using a graph transformer encoder at the transmitter, which can extract the semantic representations through the cross-modal attention mechanisms. Next, we leverage a graph semantic validation mechanism to guarantee the exactness of the overall data at the receiver. Furthermore, we adopt generative AI to regenerate multimodal data in virtual scenarios. Simultaneously, a novel 3D generative reconstruction network is constructed from the 3D point clouds, which can transfer the data from images to 3D models, and we infer the multimodal data into the 3D models to increase realism in virtual scenarios. Finally, the experiment results demonstrate that cross-modal graph semantic communication, assisted by generative AI, has substantial potential for enhancing user interactions in the 6G communications and Metaverse.

关键词： modal frontier guarantee

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Inverting the rock mass P-wave velocity field ahead of deep buried tunnel face while borehole drilling

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International Journal of Mining Science and Technology 2024年第5期34卷 681-697页

作者： Liu Liu Shaojun Li Minzong Zheng Dong Wang minghao Chen Junbo Zhou Tingzhou Yan Zhenming Shi State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan 430071China China Railway Eryuan Engineering Group CO. LTD.Chengdu 610031China Department of Hydraulic and Hydropower Engineering Tsinghua UniversityBeijing 100084China Hubei Communications Planning and Design Institute CO. LTD.Wuhan 430051China Key Laboratory of Geotechnical and Underground Engineering of Ministry of Education Tongji UniversityShanghai 200092China

Imaging the wave velocity field surrounding a borehole while drilling is a promising and urgently needed approach for extending the exploration range of the borehole *** paper develops a drilling process detection(DPD)system consisting of a multifunctional sensor and a pilot geophone installed at the top of the drilling rod,geophones at the tunnel face,a laser rangefinder,and an onsite computer.A weighted adjoint-state first arrival travel time tomography method is used to invert the P-wave velocity field of rock mass while borehole drilling.A field experiment in the ongoing construction of a deep buried tunnel in southwestern China demonstrated the DPD system and the tomography ***-frequency analysis of typical borehole drilling detection data shows that the impact drilling source is a pulse-like seismic exploration wavelet.A velocity field of the rock mass in a triangular area defined by the borehole trajectory and geophone receiving line can be *** the borehole core and optical image validate the inverted P-wave velocity field.A numerical simulation of a checkerboard benchmark model is used to test the tomography *** rapid convergence of the misfits and consistent agreement between the inverted and observed travel times validate the P-wave velocity imaging.

关键词： Deep buried tunnel Wave velocity field Borehole drilling Tomography Rock mass

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New insights into the DPR mechanism of elastic energy released by polymer gel for enhanced oil recovery

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Petroleum 2024年第3期10卷 539-547页

作者： Hu Jia Junyi Wu Shaowei Wu Yanbin Liang minghao Wang Xiaojin Wan Pengwu Li State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengduChina CNOOC Zhanjiang Branch ZhanjiangChina Oil Production Technology Institute of PetroChina Dagang Oilfield Company TianjinChina

Polymer gel was widely used as water shutoff agent in mature oil *** the results of single-phase plugging experiments show that the plugging rate of the polymer gel to the oil phase is lower than that of the water ***,the disproportionate permeability reduction(DPR)mechanism of polymer gels still remains *** this paper,we used four gel formulations including polyethyleneimine(PEI)and phenol-formaldehyde crosslinked gel with and without adding laponite to investigate the effect of gel elastic property on the water shutoff *** result of sand pack flooding experiments shown that the gel with higher elastic modulus has better effects on decreasing water cut and increasing oil *** adding laponite,the elastic modulus of phenol-formaldehyde crosslinked gel increased from 64.2 Pa to 192 Pa,and the elastic modulus of PEI crosslinked gel increased from 27.4 Pa to 36.5 *** to the phenol-formaldehyde-HAPM gel,the oil recovery of laponite-phenol-formaldehyde-HPAM gel increased by 5.2%and the maximum water cut decreased by 8.3%.Besides,comparing with PEI-HPAM gel,the oil recovery of laponite-PEI-HPAM gel increased by 2.7%and the water cut dropped by 27.8%.In the meanwhile,the laponite-phenol-formaldehyde-HPAM gel with higher elastic modulus obviously swells in the formation water but almost remains constant in oil at 105℃.The mass of gel soaked in the formation water increased from 42 g to 96 g and the gel volume increased by 300%within 48 *** study improves the understanding of the DPR mechanism of polymer gel for water shutoff.

关键词： Gel elasticity Water shutoff DPR Laponite Oil recovery

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酸催化剂对水性氨基烤漆性能的影响

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涂料工业 2023年第8期53卷 55-61页

作者：谭铭浩朱娇周盛民广东迪爱生彤德树脂有限公司鹤山分公司广东鹤山529700

酸催化剂能够让氨基烤漆在较低的温度或较短的时间快速反应,为了解不同的酸催化剂对水性氨基烤漆性能的影响,更有效地进行配方设计。使用封闭型对甲苯磺酸、十二烷基苯磺酸、封闭型十二烷基苯磺酸、二壬基萘磺酸、二壬基萘二磺酸5种酸... 详细信息

酸催化剂能够让氨基烤漆在较低的温度或较短的时间快速反应,为了解不同的酸催化剂对水性氨基烤漆性能的影响,更有效地进行配方设计。使用封闭型对甲苯磺酸、十二烷基苯磺酸、封闭型十二烷基苯磺酸、二壬基萘磺酸、二壬基萘二磺酸5种酸催化剂分别加入不同类型的水性氨基烤漆中,测试氨基烤漆的黏度、贮存稳定性,以及对比150℃和200℃2种烘烤条件下漆膜的铅笔硬度、耐冲击性、耐溶剂性、耐水煮性的差异。结果表明:封闭型十二烷基苯磺酸在提升漆膜硬度和耐溶剂性的同时,对黏度、漆膜的外观、耐冲击性、耐水煮性以及贮存稳定性的影响都较小;封闭型对甲苯磺酸对硬度和耐溶剂性提升效果明显,但不利于漆膜的外观、耐冲击性和耐水煮性。

关键词：酸催化剂氨基烤漆水性树脂性能

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Tailored multifunctional hydrazine derivatives for efficient printable hole-conductive-free mesoscopic perovskite solar cells via enhancing defect passivation

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Journal of Energy Chemistry 2024年第8期95卷 357-363,I0008页

作者： minghao Xia Ziwei Zheng Yanjie Cheng Chaoyang Wang Zhaozhen Cui Guodong Zhang Jinwei Gonga Anyi Mei Hongwei Han Michael Grätzel Center for Mesoscopic Solar Cells Wuhan National Laboratory for OptoelectronicsKey Laboratory of Materials Chemistry for Energy Conversion and Storage of Ministry of EducationHuazhong University of Science and TechnologyWuhan 430074HubeiChina Key Laboratory of Flexible Electronics(KLoFE)&Institute of Advanced Materials(IAM) School of Flexible Electronics(Future Technologies)Nanjing Tech University(NanjingTech)Nanjing 211816JiangsuChina

The low-cost and easy large-scale fabrication advantages of printable mesoscopic perovskite solar cells(p-MPSCs)are overshadowed by their limited photovoltaic conversion efficiency(PCE).Here,we introduce the hydrazide derivative of 4-Hydroxybenzoylhydrazine(4-HBH)to improve the PCE of p-MPSCs by inducing enhanced defect *** carbonyl and hydrazine groups in hydrazide groups present strong interaction with *** hydroxyl group,as an electron donor group,increases the electron cloud density of the hydrazide group in 4-HBH under the conjugation of the benzene ring,and thus enhances its interaction with ***,the hydroxy group itself interacts with perovskite and passivates defects *** hydrazine agents can also reduce I2and suppress the loss of iodine in perovskite films,which inhibits the formation of iodine-related ***,p-MPSCs with 4-HBH achieve a high PCE of 19.21%,and present well improved stability.

关键词： Printable mesoscopic perovskite solar cells Multifunctional hydrazine derivatives Cooperative coordination Conjugation redox activity

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The current status and reflections on 3D in vitro modeling of liver metastasis in colorectal cancer

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Hepatobiliary Surgery and Nutrition 2024年第1期13卷 180-183页

作者： Bo Zhou minghao Sun Mian Yang Wei Cui Huayu Yang Department of General Surgery Ningbo Medical Center Lihuili Hospital(The Affiliated Lihuili Hospital of Ningbo University)NingboChina Department of Liver Surgery Peking Union Medical College Hospital(PUMCH)Peking Union Medical College(PUMC)&Chinese Academy of Medical Sciences(CAMS)BeijingChina

Colorectal cancer(CRC)ranks as the third most prevalent cancer worldwide and is identified as the second leading cause of cancer-related fatalities(1).The liver is the most common site for distant metastasis in CRC,with approximately 20%to 25%of newly diagnosed patients experiencing liver *** the disease progresses,this proportion can increase to as high as 50%(2),making it a primary cause of death in CRC patients.

关键词： Colorectal cancer(CRC) preclinical model 3D bioprinting chemotherapy predict

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qSTA2-2,a novel QTL that contributes to seed starch synthesis in Zea mays L.

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Journal of Integrative Agriculture 2024年第4期23卷 1118-1133页

作者： minghao Cai Xuhui Li Zhi Liang Jie Wang Delin Li Zhipeng Yuan Riliang Gu Jianhua Wang Li Li State Key Laboratory of Maize Bio-breeding/Key Laboratory of Crop Heterosis Utilization Ministry of Education/Beijing Innovation Center for Crop Seed Technology/College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Institute of Nanfan&Seed Industry Guangdong Academy of ScienceGuangzhou 510316China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

The seed storage materials accumulate during seed development,and are essential for seed germination and seedling *** we employed two bi-parental populations of an F2:3 population developed from a cross of improved 220(I220,small seeds with low starch)and PH4CV(large seeds with high starch),as well as recombinant-inbred lines(RILs)of X178(high starch)and its improved introgression line I178(low starch),to identify the genes that control seed storage *** identified a total of 12 QTLs for starch,protein and oil,which explained 3.44-10.79%of the phenotypic *** them,qSTA2-1 identified in F2:3 and qSTA2-2 identified in the RILs partially overlapped at an interval of 7.314-9.554 Mb,and they explained 3.44-10.21%of the starch content variation,so they were selected for further *** mapping of qSTA2-2 with the backcrossed populations of ^(I220)/PH4CV in each generation narrowed it down to a 199.7 kb interval that contains 14 open reading frames(ORFs).Transcriptomic analysis of developing seeds from the near-isogenic lines(NILs)of ^(I220)/PH4CV(BC_(5)F_(2))showed that only 11 ORFs were expressed in 20 days after pollination(DAP)*** of them were upregulated and six of them were downregulated in NIL^(I220),and the differentially expressed genes(DEGs)between NIL^(I220) and NIL^(PH4CV) were enriched in starch metabolism,hormone signal transduction and glycosaminoglycan *** the eleven NIL^(I220) differential expressed ORFs,ORF4(Zm00001d002260)and ORF5(Zm00001d002261)carry 75%protein sequence similarity,both encodes an glycolate oxidase,were the possible candidates of *** analysis and validation indicated that mutation of the qSTA2-2 locus resulted in the dysfunction of ABA accumulation,the embryo/endosperm ratio and the starch and hormone levels.

关键词： QTL mapping seed starch transcriptomic analysis hormone

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Predicting the Bioaccessibility of Soil Cd,Pb,and As with Advanced Machine Learning for Continental-Scale Soil Environmental Criteria Determination in China

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Environment & Health 2024年第9期2卷 631-641页

作者： Kunting Xie Jiajun Ou minghao He Weijie Peng Yong Yuan Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control School of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China School of Automation Guangdong University of TechnologyGuangzhou 510006China Health Risk Control School of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China

Investigating the bioaccessibility of harmful inorganic elements in soil is crucial for understanding their behavior in the environment and accurately assessing the environmental risks associated with *** batch experimental methods and linear models,however,are time-consuming and often fall short in precisely quantifying *** this study,using 937 data points gathered from 56 journal articles,we developed machine learning models for three harmful inorganic elements,namely,Cd,Pb,and *** thorough analysis,the model optimized through a boosting ensemble strategy demonstrated the best performance,with an average R2 of 0.95 and an RMSE of *** further employed SHAP values in conjunction with quantitative analysis to identify the key features that influence *** utilizing the developed integrated models,we carried out predictions for 3002 data points across China,clarifying the bioaccessibility of cadmium(Cd),lead(Pb),and arsenic(As)in the soils of various sites and constructed a comprehensive spatial distribution map of China using the inverse distance weighting(IDW)interpolation *** on these findings,we further derived the soil environmental standards for metallurgical sites in *** observations from the collected data indicate a reduction in the number of sites exceeding the standard levels for Cd,Pb,and As in mining/smelting sites from 5,58,and 14 to 1,24,and 7,*** research offers a precise and scientific approach for cross-regional risk assessment at the continental scale and lays a solid foundation for soil environmental management.

关键词： machine learning soil environmental criteria potentially harmful elements bioaccessibility sites

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