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Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis,a Potential Biomass Crop

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Journal of Integrative Plant Biology 2011年第3期53卷 232-245页

作者： Hua Zhao Jiangyan Yu Frank M. You mingcheng luo Junhua Peng Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical Garden the Chinese Academy of Sciences Wuhan 430074 China Department of Plant Sciences University of California Davis CA 95616 USA Department of Soil and Crop Sciences Colorado State University Fort Collins CO 80523-1170 USA

Miscanthus sinensis has high biomass yield and contributed two of the three genomes in M. x giganteus, a bioenergy crop widely studied in Europe and North America, and thus is a potential biomass crop and an important germplasm for Miscanthus breeding. Molecular markers are essential for germplasm evaluation, genetic analyses and new cultivar development in M. sinensis. In the present study, we reported transferability of simple sequence repeat （SSR） markers from Brachypodium distachyon to M. sinensis. A set of 57 SSR markers evenly distributed across the B. distachyon genome were deliberately designed. Out of these B. distachyon SSR markers, 86.0% are transferable to M. sinensis. The SSR loci amplified in M. sinensis were validated by re-sequencing the amplicons. The polymorphism information content （PIC） of the transferable SSR markers varied from 0.073 to 0.375 with a mean of 0.263, assessed based on 21 M. sinensis genotypes. Phylogenetic tree based on 162 alleles detected by 49 SSR markers could unambiguously distinguish B. distachyon from M. sinensis, and cluster 21 M. sinensis genotypes into three groups that are basically in coincidence with their geographical distribution and ecotype classifications. The markers developed by the comparative genomic approach could be useful for germplasm evaluation, genetic analysis, and marker-assisted breeding in Miscanthus.

关键词： BRACHYPODIUM MISCANTHUS MICROSATELLITES (Genetics) GENETIC markers ENERGY crops GENETIC transformation BIOMASS production

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Valence electronic engineering of superhydrophilic Dy-evoked Ni-MOF outperforming RuO_(2) for highly efficient electrocatalytic oxygen evolution

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Journal of Energy Chemistry 2024年第3期90卷 244-252,I0007页

作者： Zhiyang Huang Miao Liao Shifan Zhang Lixia Wang mingcheng Gao Zuyang luo Tayirjan Taylor Isimjan Bao Wang Xiulin Yang Guangxi Key Laboratory of Low Carbon Energy Materials School of Chemistry and Pharmaceutical SciencesGuangxi Normal UniversityGulin 541004GuangxiChina State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Saudi Arabia Basic Industries Corporation(SABIC)at King Abdullah University of Science and Technology(KAUST) Thuwal 23955-6900Saudi Arabia

Tackling the problem of poor conductivity and catalytic stability of pristine metal-organic frameworks(MOFs) is crucial to improve their oxygen evolution reaction(OER) ***,we introduce a novel strategy of dysprosium(Dy) doping,using the unique 4f orbitals of this rare earth element to enhance electrocatalytic activity of *** method involves constructing Dy-doped Ni-MOF(Dy@Ni-MOF) nanoneedles on carbon cloth via a Dy-induced valence electronic perturbation *** and density functional theory(DFT) calculations reveal that Dy doping can effectively modify the electronic structure of the Ni active centers and foster a strong electronic interaction between Ni and *** resulting benefits include a reduced work function and a closer proximity of the d-band center to the Fermi level,which is conducive to improving electrical conductivity and promoting the adsorption of oxygen-containing ***,the Dy@Ni-MOF achieves superhydrophilicity,ensuring effective electrolyte contact and thus accelerating reaction kinetics,Ex-situ and in-situ analysis results manifest Dy_(2)O_(3)/NiOOH as the actual active ***,Dy@Ni-MOF shows impressive OER performance,significantly surpassing ***,the overall water splitting device with Dy@NiMOF as an anode delivers a low cell voltage of 1.51 V at 10 mA cm^(-2) and demonstrates long-term stability for 100 h,positioning it as a promising substitute for precious metal catalysts.

关键词： Dy@Ni-MOF Dy incorporation Electronic interaction Superhydrophilicity Oxygen evolution reaction

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A bacterial artificial chromosome-based physical map of Manihot esculenta ssp. flabellifolia

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Frontiers of Agricultural Science and Engineering 2016年第4期3卷 321-329页

作者： Yuhua FU Zhiqiang XIA Shujuan WANG Xin CHEN Cheng LU mingcheng luo Hongbin ZHANG Wenquan WANG Institute of tropical Biosciences & Biotechnology Chinese Academy of Tropical Agricultural Sciences Guizhou Institute of Subtropical Crops Guizhou Academy of Agricultural Sciences Department of Plant Sciences UC Davis Department of Soil and Crop Sciences Texas A&M University College Station

Cassava(Manihot esculenta) is known as the third most important food crop in the tropics and also used for industrial feedstock for biofuels. Two new bacterial artificial chromosome(BAC) libraries were constructed for W14(M. Esculenta ssp. flabellifolia), a wild ancestor of domesticated cassava. The libraries were constructed with EcoR I and Hind III insertion vectors, *** EcoR I library has 29952 clones with an average insert size of 115 kb, while the Hind III library consists of 29952 clones with an average insert of 129 kb. The combined libraries contain a total of 59904 clones with an average insert size of 125 kb, representing approximately 10 ?haploid genome equivalents. A total of 29952 clones were fingerprinted and resulted in a cassava physical map composed of 2485 contigs with an average physical length of 336 kb and 2909 singletons, representing approximately762 Mb of the cassava genome. 5000 clones located at the ends of BAC contigs were selected and sequenced. A total of 6077 SNPs and 231 indels were identified, that covered459 gene sequences, of which 6 genes were associated with starch and sucrose metabolism. This BAC-based physical map provides valuable tools to understand the genetics and evolution of cassava.

关键词： cassava BAC library physical map

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CYTOMIXIS AND CELL FUSION OF POLLEN CELLS IN F1 HYBRID OF WHEAT CROSSED WITH RYE, WITH REFERENCE TO THE FORMATION OF ANEUPLOIDS

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四川农业大学学报 1992年第4期 616-622页

作者： mingcheng luo, Chi Yen and Junliang Yang (Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, Sichuan, China, Zip Code 611830)

Cytomixis refers to the migration of chromatin from one cell to an adjacent one. The cytological investigation demonstrated that cytomixis and direct fusion of two cells occurred in early stage of pollen development, and resulted from genetic controlled behaviour in hybrid plants of wheat (Triticum aestivum L. cv. Xiaoyuer mai) crossed with rye (Secale cereale L. cv. Winter rye). The frequency of occurrences of cytomixis and cell fusion was about 10％. Spontaneous seed settings in the hybrid plants were obtained.

关键词： Cytomixis: Meiosis Aneuploid: Wheat Rye

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THE STUDY ON ESTERASE AND PEROXIDASE ISOZYMES OF WHEAT LANDRACES FROM SHAANXI, CHINA

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四川农业大学学报 1992年第4期 640-645页

作者： Zuli Yang, mingcheng luo and Chi Yen (Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, Sichuan, China, Zip Code 611830)

The esterase and peroxidase isozymes of 63 landraces of wheat (Triticum aestivum L.) from Shaanxi province, China have been studied. The results showed as follows:Significant differences of either esterase or peroxidase isozymes were detected in both roots and shoots of seedling among landraces. Eand Ewere special for roots; E, E, E, Eand Eonly appeared in shoots. 2 types of isozyme patterns of esterase were involved in roots, but 4 types in shoots. No variation of peroxidase isozymes appeared in roots, but 3 variations arose in shoots. The roots showed more zymograms and more activity than the shoots in peroxidase isozyme.

关键词： Isozyme Esterase peroxidase Wheat Electrophoresis

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QFP芯片外观视觉检测系统及检测方法

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中国机械工程 2013年第3期24卷 290-294,301页

作者：郑金驹李文龙王瑜辉罗明成华中科技大学数字制造装备与技术国家重点实验室武汉430074

基于QFP芯片的管脚外观检测的需要,开发了基于机器视觉的QFP芯片外观检测系统,并简要介绍了该系统的硬件系统和软件系统。为实现管脚外观检测要求,采用Canny算子边缘检测算法针对QFP管脚的图像进行处理,着重论述了管脚栈高的检测方法及... 详细信息

基于QFP芯片的管脚外观检测的需要,开发了基于机器视觉的QFP芯片外观检测系统,并简要介绍了该系统的硬件系统和软件系统。为实现管脚外观检测要求,采用Canny算子边缘检测算法针对QFP管脚的图像进行处理,着重论述了管脚栈高的检测方法及基于三点法的管脚共面度的检测方法。测试结果表明,该外观检测系统能达到在线生产的工艺要求。

关键词： QFP外观检测机器视觉 Canny算子三点法

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FAST主动反射面控制系统中节点控制器记录的设计

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核技术 2014年第1期37卷 38-43页

作者：吴文庆罗明诚刘佳靖唐鹏毅王坚核探测与核电子学国家重点实验室中国科学技术大学近代物理系安徽省物理电子学重点实验室合肥230026

主动反射面是FAST(Five-hundred-meter Aperture Spherical radio Telescope)望远镜的一个重大创新之一,其性能将直接关系到整个望远镜的性能,因此主动反射面的控制需要一个实时高效的控制系统。基于EPICS(Experimental Physics and Ind... 详细信息

主动反射面是FAST(Five-hundred-meter Aperture Spherical radio Telescope)望远镜的一个重大创新之一,其性能将直接关系到整个望远镜的性能,因此主动反射面的控制需要一个实时高效的控制系统。基于EPICS(Experimental Physics and Industrial Control System)的输入输出控制器(Input/Output Controller,IOC)框架,充分利用EPICS的实时性,根据FAST反射面的控制节点达2 000多个的特点设计了新的记录类型——节点控制器记录,以便于控制器节点IOC的管理以及提高代码的复用,并针对FAST主动反射面密云模型对实际装置进行了仿真、设计和部分测试。

关键词： 500米单口径球面射电天文望远镜(FAST) EPICS(ExperimentalPhysics andIndustrialControl System) 记录设计输入输出控制器(Input 0u[put Controller IOC)

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Draft genome sequence of Triticum urartu,the progenitor of wheat A genome

Draft genome sequence of Triticum urartu,the progenitor of w...

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第十四届全国植物基因组学大会

作者： Hongqing Ling Shancen Zhao Dongcheng Liu Junyi Wang Hua Sun Jan Dvorak mingcheng luo Zhensheng Li Daowen Wang Aimin Zhang Jun Wang State Kay Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences BGI Shenzhen Department of Plant Sciences University of California

Bread wheat(Triticum aestivum),one of the most widely cultivated and consumed food crops in the world,is a hexaploid containing A,B and D *** to its complex polyploidy nature and large genome size(17 Gb),the genetic and functional analysis of bread wheat is extremely *** A genome,originally from the diploid wild einkorn wheat Triticum urartu with a genome size about 5 Gb,is the basic genome of bread wheat and other polyploidy wheats,and plays a central role in wheat evolution,domestication and genetic improvement. We sequenced *** accession G1812 using a whole-genome shotgun strategy on

关键词： genome sequencing wheat Triticum urartu draft genome

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Sequencing of wheat a genome and its physical mapping

Sequencing of wheat a genome and its physical mapping

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2013全国植物生物学大会

作者： LING Hongqing ZHAO Shancen LIU Dongcheng WANG Junyi SUN Hua Jan Dvorak luo mingcheng Michiel van Eijk LI Zhensheng WANG Daowen ZHANG Aimin WANG Jun State Kay Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences BGI Shenzhen Department of Plant Sciences University of California Keygene N.V. Wageningen

Bread wheat(Triticum aestivum),one of the most widely cultivated and consumed food crops in the world,is a hexaploid containing A,B and D *** to its complex polyploidy nature and large genome

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粒子加速器中高精度丝线绝对位置测量技术研究

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测绘通报 2019年第9期 44-50页

作者：梁静罗明成王铜董岚罗涛柯志勇王小龙中国科学院高能物理研究所北京100049 东莞中子科学中心广东东莞523803

粒子加速器中采用振动线技术进行共架单元上多块磁铁的高精度预准直,需要将振动线代表的磁中心引出进行共架单元之间的相对准直。为了获取以振动线为代表的丝线高精度空间绝对位置,本文对采用近景摄影测量技术进行点、线位置求解的方法... 详细信息

粒子加速器中采用振动线技术进行共架单元上多块磁铁的高精度预准直,需要将振动线代表的磁中心引出进行共架单元之间的相对准直。为了获取以振动线为代表的丝线高精度空间绝对位置,本文对采用近景摄影测量技术进行点、线位置求解的方法进行了研究。利用单相机多站位的方法进行拍摄,通过标定板提供的高精度控制点坐标,采用自标定光束法平差对相机进行标定,并提出将空间直线离散成空间2点进行直线整体精确求解。对标定板上由2点组成的模拟直线进行测量验证,并与标定板提供的理论值进行对比,结果显示空间直线位置获取精度优于5μm。这表明了本文理论和方法的正确性,为进一步研究丝线高精度空间绝对位置测量打下了基础。

关键词：摄影测量空间直线标定板相机标定光束法平差

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