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Chinese Medical Journal 2024年第12期137卷 1507-1509页

作者： Feiyue Xiao Pengcheng Li Hang Song mingchang zhang Youxin Chen Chan Zhao Department of Ophthalmology Peking Union Medical College HospitalChinese Academy of Medical SciencesBeijing 100005China Key Laboratory of Ocular Fundus Diseases Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100005China Department of Ophthalmology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China

To the Editor:Anterior chamber(AC)paracentesis is a commonly performed procedure to lower intraocular pressure(IOP),and to sample aqueous humor(AH)for clinical and research *** suggested by an international collaboration team,an immediately performed AC paracentesis before an intravitreal injection can also prevent post-injection IOP spike.[1]While IOP could immediately be reduced by AC paracentesis,the procedure of obtaining an AH sample(liquid biopsy)is more *** methods have been developed to facilitate AH collection and transfer,[2-7]among which a vacuum-based AH collector(SnovoDAHC I-50,Sightnovo Medical Technology Co.,Beijing,China)has better performance than the commonly used insulin syringe in accurate sampling of about 50μL AH in rabbits by one hand.[7]The current clinical trial aimed to evaluate the performance and safety of this product in human subjects.

关键词： vacuum injection clinical

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角膜缘微环境细胞的分离培养研究进展

中华实验眼科杂志 2024年第4期42卷 387-392页

作者：肖宇婷(综述) 谢华桃张明昌(审校) 华中科技大学同济医学院附属协和医院眼科武汉430022

角膜缘微环境细胞(LNCs)是位于角膜缘、与角膜缘上皮干细胞(LESCs)紧密相连的一类间充质干细胞,能同时表达间充质标志物和多种胚胎干细胞标志物,对调节LESCs的静息、自我更新以及分化状态起着重要作用。近年研究表明,LNCs可通过胶原酶... 详细信息

角膜缘微环境细胞(LNCs)是位于角膜缘、与角膜缘上皮干细胞(LESCs)紧密相连的一类间充质干细胞,能同时表达间充质标志物和多种胚胎干细胞标志物,对调节LESCs的静息、自我更新以及分化状态起着重要作用。近年研究表明,LNCs可通过胶原酶消化法、上皮块消化法、中性蛋白酶-胶原酶消化法以及组织块培养法进行体外分离培养,通过3D Matrigel共培养、Transwell共培养可研究LNCs与LESCs之间的相互作用关系。LNCs与LESCs可通过SDF-1/CXCR4、Notch、BMP、Wnt、Sonic Hedgehog、KIT/AKT等多种信号通路以及神经生长因子、角质形成细胞因子、胰岛素样生长因子等多种细胞因子相互作用。LNCs现已成为角膜上皮组织工程、眼表重建以及角膜再生等研究中的一大热点。本文就LNCs的研究背景、体外分离培养方法、与LESCs的相互作用机制及其应用前景等进行综述。

关键词：角膜角膜缘微环境细胞角膜缘上皮干细胞角膜缘干细胞缺乏症组织工程眼表重建角膜再生

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甲状腺癌术后应用加味生脉散的治疗效果

河南医学研究 2024年第5期33卷 909-914页

作者：彭秀山张国斌张明昌郑州市第一人民医院普外科河南郑州450000 许昌市中心医院乳腺外科河南许昌461001 许昌中医院中医科河南许昌461001

目的甲状腺癌(TC)术后应用加味生脉散的治疗效果。方法选取2018年1月至2022年12月郑州市第一人民医院收治的60例TC术后患者,按随机数字表法分组,各30例。对照组接受常规西药治疗,观察组基于对照组加用加味生脉散,连续治疗3个月。统计两... 详细信息

目的甲状腺癌(TC)术后应用加味生脉散的治疗效果。方法选取2018年1月至2022年12月郑州市第一人民医院收治的60例TC术后患者,按随机数字表法分组,各30例。对照组接受常规西药治疗,观察组基于对照组加用加味生脉散,连续治疗3个月。统计两组临床疗效、中医证型评分、PI3K-AKT信号通路、甲状腺激素、不良反应。结果观察组治疗3个月后总有效率较对照组高(P<0.05);治疗1、3个月后观察组气短懒言、口咽干燥、疲倦乏力、五心烦热积分均低于对照组(P<0.05);治疗1、3个月后观察组促甲状腺激素、甲状腺球蛋白抗体、甲状腺过氧化物酶抗体水平低于对照组,游离三碘甲状腺原氨酸、游离甲状腺素水平及外周血单个核细胞PI3Kp110、AKT1 mRNA、蛋白均高于对照组(P<0.05)。结论TC术后应用加味生脉散效果确切,有助于改善中医证型及甲状腺功能,其作用机制可能与调控PI3K-AKT信号通路,促进细胞凋亡有关。

关键词：甲状腺癌术后加味生脉散疗效 PI3K-AKT信号通路

Clinical efficacy of 0.1% pranoprofen in treatment of dry eye patients：a multicenter, randomized, controlled clinical trial

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Chinese Medical Journal 2014年第13期127卷 2407-2412页

作者： Chen Jingyao Dong Fei Chen Wei Sun Xuguang Deng Yingping Hong Jing zhang mingchang Yang Wenzhao Liu Zuguo Xie Lixin Eye Institute of )~iamen University Medical College of XiamenUniversity Xiamen Fujian 361102 China Affiliated Eye Hospital of Wenzhou Medical Collage WenzhouZhejiang 325072 China Beijing Institute of Ophthalmology Department of OphthalmologyBeijing Tongren Hospital Capital Medical University Beijing100730 China West China School of Medicine Sichuan University ChengduSichuan 610041 China Department of Ophthalmology Peking University Third HospitalBeijing 100191 China Huazhong University of Science and Technology Wuhan Hubei430022 China Shandong Eye Institute Qingdao Shandong 266071 China

Background Dry eye is a multifactorial disease of the tears and the ocular *** study aimed to investigate the clinical efficacy of a non-steroidal anti-inflammatory drug,pranoprofen,in the treatment of dry *** It is a prospective,multi-center,randomized,controlled,parallel group *** hundred and fifteen patients with mild to moderate dry eye disease （55-60 in each treatment group） participated in this multi-center *** were randomly administered with eyedrops containing 0.1％ pranoprofen （PRA） plus 0.1％ sodium hyaluronate （SH） or SH only,three times daily for 28 days,followed by a 1-week after treatment *** eye symptom score （DESS）,fluorescein corneal staining （FLCS）,tear break-up time （TBUT）,and Shirmer 1 tear test （ST1,without anesthesia） were evaluated or conducted before treatment and at each study *** impression cytology was taken from the patients treated with PRA plus SH before and after treatment and real-time polymerase chain reaction （RT-PCR） was performed to detect the changes of human leukocyte antigen DR （HLA-DR） and intercellular adhesion molecule 1 （ICAM-1）.Results Patients treated with PRA plus SH showed gradual improvements of DESS,FLCS,and ***-group comparisons of FLCS and TBUT have statistically significant differences from day *** tolerance with no severe adverse events was found in both *** treated with PRA plus SH had a reduced expression level of HLA-DR and were statistically different after 28 days of *** The application of PRA at a dose of 0.1％ was well tolerated and benefited to the patients with mild to moderate dry eye *** underlying mechanism of its efficacy may be associated with the reduction of inflammatory factors of conjunctival epithelial cells.

关键词： dry eye nonsteroidal anti-inflammatory drug inflammation treatment conjunctival impression cytology

Mutations of Desmoglein‑2 in Sudden Unexplained Death in the Chinese Han Population

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Journal of Forensic Science and Medicine 2019年第2期5卷 61-64页

作者： Junyi Lin Yulei Yang Ziqin Zhao Yiwen Shen Kaijun Ma mingchang zhang Department of Forensic Medicine School of Basic Medical SciencesFudan UniversityShanghaiChina2Shangha Key

Sudden unexplained death(SUD)remains a puzzle in forensic ***‑2(DSG2)has been linked to arrhythmogenic right ventricular cardiomyopathy which may cause life‑threatening ventricular arrhythmias and sudden *** arrhythmias resulting in sudden death also occur in the absence of morphologic cardiac abnormalities at *** hypothesized that DSG2 mutations may be responsible for certain Chinese SUD *** sequenced all 15 exons of DSG2 in DNA extracted from postmortem heart tissues of 25 Chinese patients dying from *** primers were designed using the Primer Express 3.0 *** sequencing for both sense and antisense strands was performed with a BigDye Terminator DNA sequencing kit on a 3130 Xl Genetic *** damage prediction was made using Mutation Taster,PolyPhen,and SIFT *** 2 of 25 cases of Chinese SUD samples,two DSG2 heterozygous mutations(p.P927 L and p.T1070M)were identified,and one is probably *** concluded that DSG2 mutations may be related to the occurrence of part of SUD cases in the Chinese Han population.

关键词： Desmoglein‑2 desmosomal mutation sudden cardiac death sudden unexplained death

基于Proteus仿真的高精度温度控制器设计

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北京印刷学院学报 2023年第6期31卷 53-58页

作者：张明长北京印刷学院基础教育学院北京102600

利用PT100铂电阻作为传感器搭建电桥电路,提取温度变化信号。温度信号经放大后送入16位A/D转换器LTC1864进行采样,变成数字温度值。选用Atmega16微处理器作为检测控制核心,将数字温度值再次滤波和线性化处理,实现高精度温度测量与控制... 详细信息

利用PT100铂电阻作为传感器搭建电桥电路,提取温度变化信号。温度信号经放大后送入16位A/D转换器LTC1864进行采样,变成数字温度值。选用Atmega16微处理器作为检测控制核心,将数字温度值再次滤波和线性化处理,实现高精度温度测量与控制。整个设计过程借助Proteus实时仿真调试,逼真地再现了设计思想,大大缩短了设计调试周期。

关键词： PT100铂电阻 Atmega16微处理器测量控制 Proteus仿真

Clinical Study of Diclofenac Sodium Eyedrops Used before and after Cataract Operation

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眼科学报 1999年第1期15卷 36-40页

作者： mingchang zhang, Houren WeiDepartment of Ophthalmology, Union Hospital, Tongji Medical University, Wuhan 430022 , China Department of Ophthalmology Union Hospital Tongji Medical University Wuhan 430022 China

Purpose: To determine the effect of 0.1% diclofenac sodium (DS) eyedrops made in China on preventing surgically induced miosis and inflammation. Methods: Seventy cases of cataract inpatients were randomly divided into two groups. DS eyedrops and normal saline as placebo were applied respectively 3h, 1h, 0.5h before operation and once every morning for 7 days after operation. The pupil diameter was measured five times at different stages during the extracapsular cataract extraction. The eyepain, photophobia, conjunctival injection, KP, aqueous flare and light reaction of pupil were observed once a day after operation for a week. Results: 37 eyes in DS group and 33 eyes in placebo group were included in the study. 32 of 37 eyes in DS group (86.5%) maintained mydriasis in all stages of operation and 33 eyes (89. 2%) did not show any obvious sign of inflammation. There was statistically significant difference between the two groups. No serious side effects occurred in the DS *** : The DS

关键词： DS滴眼液白内障手术炎症日童孔

Safety and efficacy of bimatoprost/timolol fixed combination in Chinese patients with open-angle glaucoma or ocular hypertension

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Chinese Medical Journal 2014年第5期127卷 905-910页

作者： Ling Zhihong zhang mingchang Hu Yizhen Yin Zhengqin Xing Yiqiao Fang Aiwu Ye Jian Chen Xiaoming Liu Dachuan Wang Yusheng Sun Wei Dong Yangceng Sun Xinghuai Department of Ophthalmology Eye and ENT Hospital Fudan University Shanghai 200031 China

Background Lowering intraocular pressure （lOP） is currently the only therapeutic approach in primary open-angle *** the fixed-combination medications are needed to achieve sufficiently low target lOP.A multicenter prospective study in the Chinese population was needed to confirm the safety and efficacy of Bimatoprost/Timolol Fixed Combination Eye Drop in *** this study,we evaluated the safety and efficacy of Bimatoprost/Timolol Fixed Combination with concurrent administration of its components in Chinese patients with open-angle glaucoma or ocular *** In this multicenter,randomized,double-masked,parallel controlled study,patients with open-angle glaucoma or ocular hypertension who were insufficiently responsive to monotherapy with either topical β-blockers or prostaglandin analogues were randomized to one of two active treatment groups in a 1：1 ratio at 11 Chinese ophthalmic ***/timolol fixed combination treatment was a fixed combination of 0.03％ bimatoprost and 0.5％ timolol （followed by vehicle for masking） once daily at 19：00 *** concurrent treatment was 0.03％ bimatoprost followed by 0.5％ timolol once daily at 19：00 *** primary efficacy variable was change from baseline in mean diurnal intraocular pressure （IOP） at week 4 visit in the intent-to-treat （ITr） *** analysis evaluated the non-inferiority of bimatoprost/timolol fixed combination to concurrent with respect to the primary variable using a confidence interval （CI） ***/timolol fixed combination was to be considered non-inferior to concurrent if the upper limit of the 95％ CI for the between-treatment （bimatoprost/timolol fixed combination minus concurrent） difference was-≤1.5 *** events were collected and slit-lamp examinations were performed to assess ***-group comparisons of the incidence of adverse events were performed using the Pearson chi-square test or Fisher＇s exact *** Of the enroll

关键词： glaucoma open-angle bimatoprost/timolol fixed combination bimatoprost

An Analysis of 1 001 Blinding Patients with Corneal Disease in 1960-1989

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眼科学报 1998年第1期14卷 48-51页

作者： mingchang zhang, Yunan He, Houren Wei, Caikeng MaiDepartment of Ophthalmology, Union Hospital of Tongji Medical University, Wuhan 430022, China Department of Ophthalmology Union Hospital of Tongji Medical University Wuhan 430022 China

Purpose:To investigate the condition and change of corneal blindness (CB) in the past 30 ***: 1 001 blinding patients of corneal disease were clinically analysed from 1960 to 1989,including etiology, sex, age, occupation, difference between the urban and rural areas ***: The male predominated. Infection stood the first cause of blinding corneal diseases, followed by trauma,malnutrition and the ***-1 keratitis was the most frequent infection in 1980s. The highest incidence of CB was 20-40 year -old. The number of CB caused by occupational truauma was more than that by ordinary trauma in 1960s,but from 1970s, the latter was more than the former. The number of CB in rural area was more than that in the urban in 1960s,but from 1970s,there was no significant difference between the two areas. The percentage of bilateral CB was gradually ***: We should try to find more effective strategies to prevent and treat CB caused by HSV-1 keratitis, pay special attention to

关键词：角膜疾病失明感染伤害营养不良

trkA激酶抑制剂对人翼状胬肉成纤维细胞凋亡的影响(英文)

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The Chinese-German Journal of Clinical Oncology 2010年第2期9卷 113-117页

作者： Chunming Zhao mingchang zhang Bangxing Huang Jie zhang Xueying Yan Department of Ophthalmology Provincial Hospital Affiliated to Shandong University Jinan 250021 China Department of Ophthalmology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Pathology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

Objective:The aim of this study was to investigate the growth inhibition effects and the mechanisms of trkA kinase inhibitor on human pterygium fibroblasts(HPF).Methods:Three normal conjunctivas and eleven pterygiums were surgically collected,trkA and p75 was examined in different tissues by *** and p75 expression was detected in HPF by immumofluorescence *** being treated with 10-80 nmol/L trkA kinase inhibitor for 24-96 h,the growth activities of HPF were studied by MTT ***-3 was inspected in HPF by spectrophotometric *** apoptosis of HPF was detected by flow ***:Expression of trkA and p75 was significantly observed in epithelium and fibroblast of pterygium *** of trkA was observed in epithelium and fibroblast of normal *** of p75 was only observed in epithelium of normal *** kinase inhibitor could effectively inhibit the growth of human pterygium fibroblasts in vitro in time and dose dependent ***-3 expression was *** rates of apoptosis were 8.26%-29.62%(P < 0.01).Conclusion:TrkA and p75 possibly participate in genesis and development of *** kinase inhibitor could induce apoptosis of human pterygium *** apoptosis through changing the ratio of trkA and p75 in fibroblasts and up-regulate Caspase-3 was probably one of its molecular mechanisms.

关键词：成纤维细胞 TrkA 细胞凋亡酶抑制剂 Caspase 免疫组织化学肉高通滤波器