检索结果-南通市图书馆

Cellular atlases of ovarian microenvironment alterations by diet and genetically-induced obesity

Science China(Life Sciences) 2024年第1期67卷 51-66页

作者： Yonghui Jiang Xueying Gao Yue Liu Xueqi Yan Huangcong Shi Rusong Zhao Zi-Jiang Chen fei Gao Han Zhao Shigang Zhao Center for Reproductive Medicine Shandong UniversityJinan 250012China Research Unit of Gametogenesis and Health of ART-Offspring Chinese Academy of Medical Sciences(No.2021RU001)Jinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250012China Shandong Key Laboratory of Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250012China Shandong Provincial Clinical Research Center for Reproductive Health Jinan 250012China National Research Center of Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250012China The Affiliated Suzhou Hospital of Nanjing Medical University Suzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215000China Center for reproductive medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200127China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of ScienceBeijing 100101China

Obesity,which can arise from genetic or environmental factors,has been shown to cause serious damages to the reproductive *** ovary,as one of the primary regulators of female fertility,is a complex organ comprised of heterogeneous cell types that work together to maintain a normal ovarian microenvironment(OME).Despite its importance,the effect of obesity on the entire ovary remains poorly *** this study,we performed ovary single-cell and nanoscale spatial RNA sequencing to investigate how the OME changed under different kinds of obesity,including high-fat diet(HFD)induced obesity and Leptin ablation induced obesity(OB).Our results demonstrate that OB,but not HFD,dramatically altered the proportion of ovarian granulosa cells,theca-interstitial cells,luteal cells,and endothelial ***,based on the spatial dynamics of follicular development,we defined four subpopulations of granulosa cell and found that obesity drastically disrupted the differentiation of mural granulosa cells from small to large antral ***,HFD enhanced follicle-stimulating hormone(FSH)sensitivity and hormone conversion,while OB caused decreased sensitivity,inadequate steroid hormone conversion,and impaired follicular *** differences can be explained by the differential expression pattern of the transcription factor ***,our study provides a powerful and high-resolution resource for profiling obesity-induced OME and offers insights into the diverse effects of obesity on female reproductive disorders.

关键词： obesity ovarian microenvironment snRNA-seq Stereo-seq Foxo1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

New Lite YOLOv4-Tiny Algorithm and Application on Crack Intelligent Detection

引用

Journal of Shanghai jiaotong university(Science) 2024年第3期29卷 528-536页

作者：宋立博费燕琼 Student Innovation Center Shanghai Jiao Tong UniversityShanghai 200240China School of Mechanical Engineering Shanghai Jiao Tong UniversityShanghai 200240China

Conforming to the rapidly increasing market demand of crack detection for tall buildings,the idea of integrating deep network technology into wall-climbing robot for crack detection is put forward in this *** the dependence and hardware requirements when deployed on such edge devices as Raspberry Pi into consideration,the Darknet neural network is selected as the basic framework for *** order to improve the inference efficiency on edge devices and avoid the possible premature over-fitting of deep networks,the lite YOLOv4-tiny algorithm is then improved from the original YOLOv4-tiny algorithm and its structure is illustrated using Netron *** images downloaded from Internet and taken from the buildings in campus are processed to form crack detection data sets,which are trained on personal computer with the AlexeyAB version of Darknet to generate weight ***,the AlexeyAB version of Darknet accelerated by NNpack package is deployed on Raspberry Pi 4B,and the crack detection experiments are carried *** characteristics,e.g.,fast speed and lower false detection rate of the lite YOLOv4-tiny algorithm,are confirmed by comparison with those of original YOLOv4-tiny *** innovations of this paper focus on the simple network structure,fewer network layers,and earlier forward transmission of features to prevent over-fitting,showing the new lite neural network exceeds the original YOLOv4-tiny network significantly.

关键词： intelligent detection deep network edge device Raspberry Pi

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comprehensive identification and potential application of genetic alteration-driven enhancer RNAs for eRNA-targeted therapy in breast cancer

引用

Genes & Diseases 2024年第5期11卷 59-62页

作者： Hongying Zhao Caiyu Zhang Lin Bo Lixia Wang Wangyang Liu Yaopeng Shu Kailai Liu Ying Liu Meiting fei Li Wang College of Bioinformatics Science and Technology Harbin Medical UniversityHarbinHeilongjiang 150081China Ninth People’s Hospital Shanghai Jiao Tong University School of MedicineShanghai 200125China Shanghai Institute of Precision Medicine Shanghai 200125China

Enhancer RNAs(eRNAs)are a class of non-coding RNA,which play a critical role in tumor progression.1 Previous studies have indicated abundant CpG methylation,somatic mutation,and copy number variation in eRNA regions in cancers.2,3 We constructed a landscape of genetic alteration-driven eRNAs and provided an integrative pipeline to identify drug candidates that affect eRNA ***,we explored the prognostic value of genetic alteration-driven eRNAs.

关键词： alteration breast targeted

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

叠氮化银起爆药连续化合成芯片的设计与应用研究

引用

含能材料 2024年第3期32卷 270-279页

作者：韩瑞山王燕兰卢飞朋张松张方李蛟褚恩义陕西应用物理化学研究所瞬态化学效应与控制全国重点实验室陕西西安710061

针对叠氮化银(AgN3,简写为SA)起爆药合成过程对于反应溶液快速混合的要求,设计制作了连续反向旋T形微混合芯片,并采用Ansys Fluent仿真模拟软件对芯片结构及反应物流速等因素对混合效率的影响规律进行了研究,优化获得了高效微混合芯片... 详细信息

针对叠氮化银(AgN3,简写为SA)起爆药合成过程对于反应溶液快速混合的要求,设计制作了连续反向旋T形微混合芯片,并采用Ansys Fluent仿真模拟软件对芯片结构及反应物流速等因素对混合效率的影响规律进行了研究,优化获得了高效微混合芯片结构。使用该芯片进行了纳米SA起爆药的连续化合成,通过扫描电子显微镜(SEM)、X射线衍射(XRD)、傅里叶变换红外光谱(FTIR)、差示扫描量热(DSC)研究了SA起爆药的形貌、成分结构与热性能。结果表明:当微混合芯片的通道尺寸为1 mm,对撞角度180°,反应物流速4 mL·min^(-1)以上时,可获得接近100%的混合效率。通过调节反应物流速、浓度和添加表面活性剂,可有效调控产物粒径及其分布,且反应产物主要成分为正交晶系的AgN3晶体。相较常规方法,使用微流控方法制备的SA起爆药放热峰温度由365.2℃提前到358.2℃(降低7℃),且放热量由851.6 kJ·kg^(-1)升高到976.7 kJ·kg^(-1)(升高14.7%),表明微流控方法制备的SA起爆药具有更高的反应活性和能量。

关键词：微流控微混合芯片起爆药叠氮化银

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prognostic factors for lacrimal gland adenoid cystic carcinoma:a retrospective study in Chinese patients

引用

International Journal of Ophthalmology(English edition) 2024年第8期17卷 1423-1430页

作者： Lu-Di Yang Shi-Chong Jia Jie Yang Xin Song Ye-fei Wang Xian-Qun Fan Department of Ophthalmology Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology Shanghai 200011China Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Visual ScienceNankai University Affiliated Eye HospitalTianjin Eye InstituteTianjin 300074China

AIM:To explore the prognostic factors for lacrimal gland adenoid cystic carcinoma(LGACC)in Chinese ***:Clinical and histopathological data were reviewed in patients with pathologically confirmed *** recurrence,metastasis,and disease-specific death were the main outcome *** and multivariate analyses were performed by the Kaplan-Meier method and a Cox proportional hazard ***:This retrospective cohort study included 45 patients with pathologically confirmed LGACC between January 2008 and June ***(T)classification(P=0.005),nodal metastasis(N)classification(P=0.018)and positive margin(P=0.008)were independent risk factors of recurrence;T(P=0.013)and N(P=0.003)classification and the basaloid tumor type(P=0.032)were independent risk factors for metastasis;T classification(P<0.001)was an independent factor of death of *** the further analysis,the durations from first surgery to radiotherapy is correlated with metastatic risk in LGACC patients with basaloid component(P=0.022).CONCLUSION:Histological subtype should be emphasized when evaluating prognosis and guiding *** radiotherapy may reduce the risk of metastasis in patients with basaloid component.

关键词： lacrimal gland adenoid cystic carcinoma risk factors prognostic analysis histological subtypes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

《东京湾城市ESG战略2022》核心观点解析及其对我国城市更新的启示

引用

城市发展研究 2024年第4期31卷 29-35页

作者：蔡颖魏晓芳吕飞封佳妮孟娇俞娅苏州科技大学建筑与城市规划学院

当今全球城镇化速度整体放缓，城市更新面临新的机遇与挑战。日本在面对气候变化和未来流行病的大背景下，将ESG理念(环境、社会、治理)引入城市更新战略，并纳入城市规划与建设中。日本政府2022年颁布了《东京湾城市ESG发展战略2022》... 详细信息

当今全球城镇化速度整体放缓，城市更新面临新的机遇与挑战。日本在面对气候变化和未来流行病的大背景下，将ESG理念(环境、社会、治理)引入城市更新战略，并纳入城市规划与建设中。日本政府2022年颁布了《东京湾城市ESG发展战略2022》,在空间治理与优化方面提出了绿色滨水空间营造、防灾减灾对策推进、数字尖端技术应用、高效创新城市营建和丰富多样交通网络五大可持续性发展战略，倡导多方参与式规划，旨在将东京湾区建设为智慧、韧性、创造性的未来城市典范，其更新思路、实施战略、参与机制等方面对我国的城市更新有着一定的启示作用。在我国城乡发展的新时期，将“ESG”理念融入城市规划治理，有助于提升城市更新的综合效益，为城市的高质量可持续发展提供新的思路与方法。

关键词：城市更新 ESG战略东京湾城市ESG

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

长期种植紫花苜蓿对复垦土壤碳氮磷养分转化的影响

引用

生态与农村环境学报 2024年第1期40卷 78-86页

作者：明玉飞朱桃川张金豪李传福高舒徐家林李永强焦树英山东农业大学资源与环境学院/土肥高效利用国家工程研究中心山东泰安271018 德州市园林绿化服务中心山东德州253000

为研究长期种植紫花苜蓿对复垦土壤质量改善和生物改土的效果,以种植作物地和撂荒地为对照,分析建筑复垦地多年种植紫花苜蓿土壤有机碳(SOC)、全氮(TN)和全磷(TP)含量的化学计量特征变化。结果表明,长期种植紫花苜蓿显著降低土壤电导率(... 详细信息

为研究长期种植紫花苜蓿对复垦土壤质量改善和生物改土的效果,以种植作物地和撂荒地为对照,分析建筑复垦地多年种植紫花苜蓿土壤有机碳(SOC)、全氮(TN)和全磷(TP)含量的化学计量特征变化。结果表明,长期种植紫花苜蓿显著降低土壤电导率(EC),对表层土壤保水效果较好,显著提升土壤有效养分含量(P<0.05);与作物地和撂荒地相比,苜蓿地土壤SOC和TN含量显著提高(P<0.05),但0~20 cm土壤TP含量显著低于作物地。3种土地利用类型0~20 cm土壤化学计量差异显著,苜蓿地土壤C/N显著低于作物地和撂荒地(P<0.05),而土壤C/P和N/P则表现为苜蓿地显著高于作物地和撂荒地(P<0.05)。种植紫花苜蓿有助于提升土壤有机碳氮活性组分,0~20 cm土层苜蓿地颗粒有机碳(POC)、易氧化有机碳(ROC)和微生物生物量碳(MBC)含量较作物地分别提高88.38%、17.24%和39.16%(P<0.05),苜蓿地颗粒有机氮(PON)、微生物生物量氮(MBN)和酸解有机氮组分含量最高,PON和MBN比作物地显著提高135.29%和17.39%,较撂荒地显著提高207.69%和28.41%。因此,长期种植紫花苜蓿对土壤质量和有机碳氮活性组分均有改善作用,研究结果可为退化土壤修复和复垦土壤生物改土效果提供参考。

关键词：紫花苜蓿土地利用类型碳氮磷化学计量土壤碳氮有机组分土壤改良

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

一种新型查耳酮衍生物C13通过ErbB4/PI3K/AKT信号通路抑制人胃癌细胞的生长

引用

药学学报 2024年第4期59卷 957-964页

作者：谭鹏张云封王龙燕黄惠铭王飞魏雪娇王柱国李军胡仲冬北京中医药大学北京中医药研究院中药现代研究中心北京100029

3ʹ-羟基-4ʹ-甲氧基-2-羟基-5-溴查耳酮(以下简称C13)是本课题组对龙血竭抗肿瘤活性化合物DHMMF进行结构修饰过程中获得的一个新型查耳酮衍生物。本研究探讨了C13对人胃癌HGC-27、AGS细胞增殖、凋亡的影响及其潜在的作用机制。首先采用... 详细信息

3ʹ-羟基-4ʹ-甲氧基-2-羟基-5-溴查耳酮(以下简称C13)是本课题组对龙血竭抗肿瘤活性化合物DHMMF进行结构修饰过程中获得的一个新型查耳酮衍生物。本研究探讨了C13对人胃癌HGC-27、AGS细胞增殖、凋亡的影响及其潜在的作用机制。首先采用噻唑蓝(methyl thiazolyl tetrazolium,MTT)、集落形成实验、5-乙炔基-2′-脱氧尿苷(5-ethynyl-2ʹ-deoxyuridine,EdU)染色法发现C13能显著抑制人胃癌HGC-27、AGS细胞的增殖能力。采用流式细胞术和免疫印迹法检测发现C13能诱导人胃癌HGC-27、AGS细胞发生凋亡,并上调剪切的聚腺苷酸二磷酸核糖转移酶(cleaved-poly ADP-ribose polymerase,cleaved-PARP)的蛋白水平。转录组测序分析结果显示C13可能通过调控人表皮生长因子受体4/磷脂酰肌醇3-激酶/蛋白激酶B(Erb-B2 receptor tyrosine kinase 4/phosphoinositide 3-kinase/AKT,ErbB4/PI3K/AKT)信号通路发挥抗胃癌作用。最后免疫印迹实验结果表明,C13处理后能下调人胃癌细胞中ErbB4总蛋白及磷酸化水平,并能够抑制其下游PI3K及AKT蛋白磷酸化水平,进一步验证了转录组测序结果。综上所述,新型查耳酮衍生物C13能够显著抑制人胃癌HGC-27、AGS细胞增殖并诱导其发生凋亡,其机制可能与下调ErbB4/PI3K/AKT信号通路有关。本研究能够为胃癌治疗提供一个候选研究药物。

关键词：查耳酮衍生物C13 人胃癌细胞增殖凋亡 ErbB4/PI3K/AKT信号通路

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Exosome-encapsulated lncRNA HOTAIRM1 contributes to PM-aggravated COPD airway remodeling by enhancing myofibroblast differentiation

引用

Science China Life Sciences 2024年

作者： Huaqi Guo Luo fei Hengyi Yu Yan Li Yan Feng Shaowei Wu Yan Wang Department of Occupational and Environmental Health Sciences School of Public Health Xi'an Jiao Tong University Health Science Center School of Public Health Shanghai Jiao Tong University School of Medicine The Ninth People's Hospital of Shanghai jiao Tong University School of Medicine

Emphysema, myofibroblast accumulation and airway remodeling can occur in the lungs due to exposure to atmospheric pollution, especially fine particulate matter (PM2.5), leading to chronic obstructive pulmonary disease (COPD). Specifically, bronchial epithelium-fibroblast communication participates in airway remodeling, which results in COPD. An increasing number of studies are now being conducted on the role of exosome-mediated cell-cell communication in disease pathogenesis. Here, we investigated whether exosomes generated from bronchial epithelial cells could deliver information to normal stromal fibroblasts and provoke cellular responses, resulting in airway obstruction in COPD. We studied the mechanism of exosome-mediated intercellular communication between human bronchial epithelial(HBE) cells and primary lung fibroblasts (pLFs). We found that PM2.5-induced HBE-derived exosomes promoted myofibroblast differentiation in p LFs. Then, the exosomal lncRNA expression profiles derived from PM2.5-treated HBE cells and nontreated HBE cells were investigated using an Agilent Human LncRNA Array. Combining coculture assays and direct exosome treatment, we found that HBE cell-derived exosomal HOTAIRM1 facilitated the myofibroblast differentiation of pLFs. Surprisingly, we discovered that exosomal HOTAIRM1 enhanced p LF proliferation to secrete excessive collagen secretion, leading to airway obstruction by stimulating the TGF-β/SMAD3 signaling ***, PM2.5reduced FEV1/FVC and FEV1 and increased the level of serum exosomal HOTAIRM1 in healthy people; moreover, serum exosomal HOTAIRM1 was associated with PM2.5-related reductions in FEV1/FVC and FVC. These findings show that PM2.5triggers alterations in exosome components and clarify that one of the paracrine mediators of myofibroblast differentiation is bronchial epithelial cellderived HOTAIRM1, which has the potential to be an effective prevention and therapeutic target for PM2.5-induced COPD.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

体外模拟消化对鲟鱼头硒螯合肽结构及抗氧化活性的影响

引用

安徽农业科学 2024年第7期52卷 169-173页

作者：贾娇吴汶飞大连工业大学食品学院辽宁大连116034 大连工业大学国家海洋食品工程技术研究中心辽宁大连116034

为了明确鲟鱼头蛋白肽和鲟鱼头硒螯合肽的抗氧化活性差异,体外模拟了口腔及胃肠道消化处理过程中鲟鱼头蛋白肽和鲟鱼头硒螯合肽的结构及抗氧化活性变化。结果表明:鲟鱼头硒螯合肽的紫外光谱吸收峰在体外口腔胃肠模拟消化过程中比鲟鱼头... 详细信息

为了明确鲟鱼头蛋白肽和鲟鱼头硒螯合肽的抗氧化活性差异,体外模拟了口腔及胃肠道消化处理过程中鲟鱼头蛋白肽和鲟鱼头硒螯合肽的结构及抗氧化活性变化。结果表明:鲟鱼头硒螯合肽的紫外光谱吸收峰在体外口腔胃肠模拟消化过程中比鲟鱼头蛋白肽更加稳定。体外模拟消化导致鲟鱼头蛋白肽和鲟鱼头硒螯合肽的圆二色谱吸收峰强度显著降低。粒径分布结果也表明:鲟鱼头硒螯合肽在消化过程中具有更好的溶解性。ABTS、DPPH以及OH自由基清除活性结果显示,在体外模拟消化后,鲟鱼头蛋白肽和鲟鱼头硒螯合肽的自由基清除活性进一步增强,且鲟鱼头硒螯合肽的自由基清除能力最强。

关键词：鲟鱼头蛋白肽硒螯合肽结构特性体外模拟抗氧化活性

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：