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Diversity and functional analysis of Chinese bumblebee gut microbiota reveal the metabolic niche and antibiotic resistance variation of Gilliamella

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Insect Science 2021年第2期28卷 302-314页

作者： Zi-Jing Zhang ming-fei huang Li-fei Qiu Rui-Hao Song Zi-Xuan Zhang Yi-Wen Ding Xin Zhou Xue Zhang Hao Zheng College of Food Science and Nutritional Engineering China Agricultural UniversityBeijingChina College of Plant Protection China Agricultural UniversityBeijingChina

Bumblebees play an important role in maintaining the balance of natural and agricultural ecosystems,and the characteristic gut microbiota of bumblebees exhibit significant mutualistic *** has the highest diversity of bumblebees;however,gut microbiota of Chinese bumblebees have mostly been investigated through cultureindependent ***,we analyzed the gut communities of bumblebees from Sichuan,Yunnan,and Shaanxi provinces in China through 16S ribosomal RNA amplicon sequencing and bacterial *** revealed that the bumblebees examined in this study harbored two gut enterotypes as previously reported:one is dominated by Gilliamella and Snodgrassella,and the other is distinguished by prevalent environmental *** gut compositions obviously varied among different individual *** then isolated 325 bacterial strains and the comparative genomic analysis of Gillianiella strains revealed that galactose and pectin digestion pathways were conserved in strains from bumblebees,while genes for the utilization of arabinose,mannose,xylose,and rhamnose were mostly *** two strains from the Chinese bumblebees possess the multidrug-resistant gene emrB,which is phylogenetically closely related to that from the symbionts of soil entomopathogenic *** contrast,tetracycline-resistant genes were uniquely present in three strains from the *** results illustrate the prevalence of strain-level variations in the metabolic potentials and the distributions of antibiotic-resistant genes in Chinese bumblebee gut bacteria.

关键词： antibiotic resistance bumblebee Gilliamella.,gut microbiota sugar metabolism

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A Mn-porphyrinic metal–organic framework immobilizing glucose oxidase for combined photodynamic/chemodynamic/starvation therapy

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Tungsten 2025年第1期7卷 183-194页

作者： Xin-Ran Sun Hao-ming Yuan Guo-Da Zhang Chao Wang Shu-Juan Sun Peng-fei Shi Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers School of Chemistry and Chemical Engineering Linyi University

The combination of photodynamic therapy（PDT） and chemodynamic therapy（CDT） in tumor treatment has attracted considerable ***,tumor hypoxia and glutathione（GSH） overproduction in the tumor tissue restricted the progress of their ***,a Mn-porphyrinic metal-organic framework（Mn-TCPP） was constructed by the one-pot method and further used for immobilizing glucose oxidase（GOx） to obtain GOx@*** would rapidly exhaust endogenous glucose into hydrogen peroxide（H2O2） and gluconic acid,thus shutting off the energy supply of tumor cells for starvation ***-TCPP catalyzed H2O2to produce oxygen,regulating the hypoxic tumor microenvironment and in turn improving1O2generation under laser ***,Mn-TCPP can reduce reactive oxygen species consumption owing to the redox reaction between Mn3+and GSH,thus greatly enhancing ***,benefiting from GOx-mediated starvation therapy,Mn2+produced by Mn3+reduction can react with sufficient intracellular H2O2to generate ·OH with high cytotoxicity through a Fenton-like *** treatment by GOx@Mn-TCPP under laser irradiation in vitro,the cell viability of 4T1 and A549 tumor cells reached to 20%,reflecting GOx@Mn-TCPP could give full play to the advantages of PDT/CDT/starvation *** results in vivo demonstrated that GOx@Mn-TCPP mediated synergistic cascade therapy could significantly inhibit tumor growth and improve the therapeutic effect.

关键词： Chemodynamic therapy Immobilizing enzyme Metal–organic frameworks Photodynamic therapy Starvation therapy

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Development of a sucrose inducible and enhancible expression and secretion system in Bacillus subtlis and investigation of the enhancive effect of degQ gene

Development of a sucrose inducible and enhancible expression...

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华东六省一市生物化学与分子生物学会2003年学术交流会

作者： ZHU Xin-Hua, Jing ming-fei, huang Jing, WU Zi-Rong (East China Normal University, ShangHai 200062)

As an attractive host for the production of heterologous secretroy proteins, Bacillus subtilis is capable of secre-ting functional proteins directly into the culture medium. But the secretion of high level of proteases is a major limi-tation. Sucrose-inducible foreign protein expression and secretion system may he a solution to this problem. Levansucrase is a sucrose-inducible secretory enzyme from Bacillus subtlis, which is encoded by sacB *** gene has the regulatory region required for sucrose induction and the signal peptide sequence for efficient se-cretion, together called sacR. Furthermore, there exists a family of regulatory factors, such as degQ gene productthat can interact with the regulatory region to enhance the expression of sacB from 10- to 100-fold at the transcrip-tional level. So, regarding the sacB regulatory region, it is feasible to develop a set of sucrose-inducible vectors forintracellular expression of heterogeny proteins. In this study, a set of sucrose-inducible expression and secretion vectors related to sacR was constructed toproduce Bacillus subtlis alkaline proteinase, by using Bacillus subtlis DB403 as the host, and Bacillus subtlis degQ

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Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第1期68卷 288-296页

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen ming-ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-fei Xu Ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

关键词： metabolon Diels-Alder reaction semipinacol rearrangement biosynthesis notoamides spiro polycyclic alkaloids

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早期巨块型宫颈癌淋巴结转移的规律及其与预后的关系

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中山大学学报（医学科学版） 2016年第5期37卷 728-732页

作者：关明飞黄萍朱安娜李从铸周莉李燕汕头大学医学院附属肿瘤医院妇科广东汕头515031

【目的】探讨早期巨块型宫颈癌患者淋巴结转移分布的规律及其与预后的关系。【方法】回顾性分析2011年1月至2015年3月在汕头大学医学院附属肿瘤医院收治的157例局部晚期宫颈癌患者(局部肿瘤>4 cm的Ib2或Ⅱa2期),收集其临床病理资料,并... 详细信息

【目的】探讨早期巨块型宫颈癌患者淋巴结转移分布的规律及其与预后的关系。【方法】回顾性分析2011年1月至2015年3月在汕头大学医学院附属肿瘤医院收治的157例局部晚期宫颈癌患者(局部肿瘤>4 cm的Ib2或Ⅱa2期),收集其临床病理资料,并随访患者生存情况。【结果】157例患者中50例(31.8%)发生淋巴结转移(LNM),以闭孔/宫旁淋巴结转移最常见;10例(6.4%)发生髂总淋巴结转移;腹主动脉旁淋巴结阳性的患者均伴有盆腔淋巴结转移。病理类型、深肌层浸润、淋巴脉管间隙浸润、宫旁受累均为淋巴结转移的独立危险因素(P2枚,预后更差(P<0.05)。【结论】早期巨块型宫颈癌以闭孔/宫旁淋巴结最易转移,淋巴结基本遵循逐级转移的规律;淋巴结转移部位越高或者数目越多,预后越差。

关键词：宫颈癌淋巴结转移腹膜后淋巴结切除预后

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Negative PO3-implanted bionic ion transport channels for promoting Li-S battery

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Science China Chemistry 2025年

作者： Wenhuan huang Shun Wang Xuehan Hou Yanan Zhang Xingxing Zhang Yaoxiao Zhao Xilang Jin Huabin Zhang fei Wang Jian Zhang Key Laboratory of Chemical Additives for China National Light Industry College of Chemistry and Chemical EngineeringShaanxi University of Science and Technology School of Materials and Chemical Engineering Xi'an Technological University Chemistry Program Physical Science and Engineering Division King Abdullah University of Science and Technology State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences

Lithium-sulfur （Li-S） battery,as a promising next-generation high-energy-density battery,suffers from lithium polysulfides（LPSs） shuttle and Li dendrites *** crystalline framework-based membranes as separator can effectively absorb and restrain LPSs but without precise structural design principal exploration and mechanism ***,bio-inspired 1D oriented lithium-ion transport channels with molecular negatively charged PO3（-1） have been developed in hybrid tetrazole frameworks for promoting the transmission of Li+*** theoretical calculations and in situ spectroscopy demonstrated the higher binding energy and inhibited diffusion of LPSs in *** assembled PP@PO3Janus separator in Li-S coin cell delivered a high initial capacity of 1410.9 mAh g-1at 0.1 C and a low attenuation rate of 0.038%per cycle over 500 cycles at 5 ***,the high capacities of 862,542 and 409 mAh g-1based on high-sulfur-loading cathodes of 2.6,3.8 and 5.0 mg cm-2at0.5 C were achieved,***,the punch battery of PO3@PP separator with S-cathode of 1.2 mg cm-2has been developed for demonstrating its potentials commercial application,which displays impressive capacity of 873.4 mAh g-1with the retention of～78.9%over 50 cycles.

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一种将Simulink/Stateflow模型改造成HLA成员的方法

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系统仿真学报 2002年第7期14卷 883-886,897页

作者：田新华冯润明翁干飞黄柯棣国防科技大学机电工程与自动化学院湖南长沙410073

介绍了将Simulink/Stateflow模型改造成HLA成员的意义、方法和过程,对Simulink/Stateflow 模型转化为HLA成员的机理,以及实现过程中的关键技术,包括模型外部接口的对象化封装、HLA外壳程序(HLA-wrapper)、基于Simulink/Stateflow的仿真... 详细信息

介绍了将Simulink/Stateflow模型改造成HLA成员的意义、方法和过程,对Simulink/Stateflow 模型转化为HLA成员的机理,以及实现过程中的关键技术,包括模型外部接口的对象化封装、HLA外壳程序(HLA-wrapper)、基于Simulink/Stateflow的仿真成员的程序结构、运行时间接口(run-time interface)调度程序的修改、模型运行时间的管理作了阐述,并描述了一个自行开发的将Simulink/Stateflow模型自动转化为HLA成员的软件工具SMIT(Simulink Model Import Tool),包括其用例描述(use-case),程序结构等。

关键词： HLA成员 Simulink/Stateflow模型 HLA外壳程序仿真系统技术改造

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食叶草蛋白的提取工艺及其性质研究

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食品与发酵工业 2025年第1期51卷 189-198页

作者：高思聪黄文选黄志超缪铭江南大学食品学院江苏无锡214122 秦皇岛森源投资集团有限公司河北秦皇岛066199

食叶草是一种潜在的优质植物蛋白质来源,该研究旨在优化食叶草蛋白的提取工艺,实现食叶草的高值化利用。本研究采用乙醇预处理结合碱溶酸沉法提取食叶草蛋白,并采用响应面试验对提取工艺进行优化。优化后的最佳工艺条件为:碱溶温度81.12... 详细信息

食叶草是一种潜在的优质植物蛋白质来源,该研究旨在优化食叶草蛋白的提取工艺,实现食叶草的高值化利用。本研究采用乙醇预处理结合碱溶酸沉法提取食叶草蛋白,并采用响应面试验对提取工艺进行优化。优化后的最佳工艺条件为:碱溶温度81.12℃,加碱量10.57 g/L,料液比1∶34.04(g∶mL),pH 3.5。在此条件下,食叶草蛋白得率为(63.26±1.01)%,纯度为(81.63±2.35)%。对食叶草蛋白结构特征进行分析,圆二色谱结果显示食叶草蛋白的α-螺旋和无规卷曲含量较高,分别为36.1%和52.5%,这可能表明其表面疏水性较高;荧光光谱显示食叶草蛋白在345 nm出现最大荧光吸收峰,表明其色氨酸残基微环境非极性较强;扫描电镜结果显示食叶草蛋白较为聚集,呈堆积状。通过考察食叶草蛋白的功能性质,发现其在碱性条件下具有良好的溶解性和乳化性,但起泡性较差。该研究将为食叶草蛋白在后续食品工业生产中的应用提供数据支撑。

关键词：食叶草叶蛋白提取工艺结构特征功能性质

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Role of nutrition in diabetes mellitus and infections

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World Journal of Clinical Cases 2025年第3期13卷 1-8页

作者： Xue-Lu Yu Li-Yun Zhou Xiao huang Xin-Yue Li ming-Ke Wang Ji-Shun Yang Naval Medical Center of PLA Naval Medical UniversityShanghai 200052China

In this editorial,we have commented on the article that has been published in the recent issue of World Journal of Clinical *** authors have described a case of unilateral thyroid cyst and have opined that the acute onset of infection may be linked to diabetes mellitus(DM).We have focused on the role of nutrition in the association between DM and *** with DM are at a high risk of infection,which could also be attributed to nutrition-related *** interventions for patients with diabetes are mainly based on a low-calorie diet,which can be achieved by adhering to a low-carbohydrate ***,dietary fiber supplementation is recommended to maintain the diversity of the gut ***,high-quality protein can prevent the increased risk of infection due to *** of vitamins C,vitamins A,vitamins D,and folic acid improves blood sugar control and facilitates immune *** deficiencies augment the risk of infection,but the relationship with diabetes is mostly U-shaped and a good intake should be maintained.

关键词： Diabetes mellitus Infection Macronutrients Vitamins Minerals

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Probability density functions of quantum mechanical observable uncertainties

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Communications in Theoretical Physics 2022年第7期74卷 84-98页

作者： Lin Zhang Jinping huang Jiamei Wang Shao-ming fei Institute of Mathematics Hangzhou Dianzi UniversityHangzhou 310018China Department of Mathematics Anhui University of TechnologyMa Anshan 243032China School of Mathematical Sciences Capital Normal UniversityBeijing 100048China

We study the uncertainties of quantum mechanical observables, quantified by the standard deviation(square root of variance) in Haar-distributed random pure states. We derive analytically the probability density functions(PDFs) of the uncertainties of arbitrary qubit *** on these PDFs, the uncertainty regions of the observables are characterized by the support of the PDFs. The state-independent uncertainty relations are then transformed into the optimization problems over uncertainty regions, which opens a new vista for studying stateindependent uncertainty relations. Our results may be generalized to multiple observable cases in higher dimensional spaces.

关键词： uncertainty of observable probability density function uncertainty region statindependent uncertainty relation

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