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Detection of telomerase activity by combination of telomeric repeat amplification protocol and electrochemiluminescence assay

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Chinese Chemical Letters 2008年第6期19卷 699-702页

作者： xiao ming Zhou li Jia MOE Key Laboratory of Laser life Science ＆ Institute of Laser life Science South China Normal University Guangzhou 510631 China

A highly sensitive telomerase detection method that combines telomeric repeat amplification protocol （TRAP） and magnetic beads based electrochemiluminescence （ECL） assay has been developed. Briefly, telomerase recognizes biotinylated telomerase synthesis primer （B-TS） and synthesizes extension products, which then serve as the templates for PCR amplification using B-TS as the forward primer and tris-（2′2′-bipyridyl） ruthenium （TBR） labeled ACX （TBR-ACX） as the reversed primer. The amplified product is captured on streptavidin-coated paramagnetic beads and detected by ECL. Telomerase positive HeLa cells were used to validate the feasibility of the method. The experimental results showed down to 10 cancer cells can be detected easily. The method is a useful tool for telomerase activity analysis due to its sensitivity, rapidity, safety, high throughput, and low cost. It can be used for screening a large amount of clinical samples.

关键词： Telomerase activity TRAP Electrochemiluminescence Cancer diagnosis

Type-Aware Question Answering over Knowledge Base with Attention-Based Tree-Structured Neural Networks

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Journal of Computer Science & Technology 2017年第4期32卷 805-813页

作者： Jun Yin Wayne Xin Zhao xiao-ming li School of Electronic Engineering and Computer Science Peking University Beijing 100871 China School of Information Renmin University of China Beijing 100872 China Guangdong Key Laboratory of Big Data Analysis and Processing Guangzhou 510006 China

Question answering (QA) over knowledge base (KB) aims to provide a structured answer from a knowledge base to a natural language question. In this task, a key step is how to represent and understand the natural language query. In this paper, we propose to use tree-structured neural networks constructed based on the constituency tree to model natural language queries. We identify an interesting observation in the constituency tree: different constituents have their own semantic characteristics and might be suitable to solve different subtasks in a QA system. Based on this point, we incorporate the type information as an auxiliary supervision signal to improve the QA performance. We call our approach type-aware QA. We jointly characterize both the answer and its answer type in a unified neural network model with the attention mechanism. Instead of simply using the root representation, we represent the query by combining the representations of different constituents using task-specific attention weights. Extensive experiments on public datasets have demonstrated the effectiveness of our proposed model. More specially, the learned attention weights are quite useful in understanding the query. The produced representations for intermediate nodes can be used for analyzing the effectiveness of components in a QA system.

关键词： question answering deep neural network knowledge base

Improved QO-STBC for three and four transmit antennas

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The Journal of China Universities of Posts and Telecommunications 2010年第4期17卷 42-46页

作者： JIA ming-ru xiao li-ping College of Information Science and Engineering Yanshan Universitys Qinhuangdao 066004 China

A new quasi-orthogonal space-time block code （QO-STBC） based on ABBA code with lower decoding complexity and higher bit error statistics （BER） performance was proposed by U. Park recently, which can obtain full transmission rate and full diversity. In this article, the authors proposed an improved U. Park scheme based on rotational ABBA code. For eliminating the interference terms resulting from neighboring signals during signal detection, two different matrixes are used. And independent decoding can be realized when maximum-likelihood decoding at the receiver is used. Simulation results demonstrate that the BER performance is improved dramatically without increasing the decoding complexity compared with U. Park scheme.

关键词： independent decoding multiple antennas QO-STBC

Remotely sensed big data for the oceans and polar regions

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Big Earth Data 2022年第2期6卷 141-143页

作者： xiao-ming li International Research Center of Big Data for Sustainable Development Goals BeijingChina Key Laboratory of Digital Earth ScienceAerospace Information Research InstituteChinese Academy of SciencesBeijingChina

The oceans,which account for 71%of the Earth’s area,and the polar regions,the largest cold source on Earth,jointly play crucial roles in energy exchange and circulation,and in climate change(***,Chapin,Walsh,&Wirth,2006).In particular,against the background of global climate change,both the Arctic and Antarctic are experiencing profound changes(Bracegirdle,Connolley,&Turner,2008;Jeffries,Overland,&Perovich,2013),and the inter-actions between the oceans,polar regions and the atmosphere are closer than *** sensing has become one of the main research tools in ocean and polar studies(Lubin,Ayres,&Hart,2009).At the same time,the amount of acquired data is undergoing explosive growth(Ma et al.,2015),thus leading oceanography into the era of“big data”.

关键词： Earth oceans Arctic

Analysis of energy consumption for lump coal degradation in melting gasifier

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Journal of Iron and Steel Research International 2017年第8期24卷 770-777页

作者： Qi-hang liu xiao-ming li Jun-xue Zhao School of Metallurgical Engineering Xi'an University of Architecture and TechnologyXi'an 710055ShaanxiChina

The volume hypothesis,a theory about the energy scale of comminution,was adopted to analyze the degradation behavior of lump coal under different heating *** breakage energy of chars was determined by a compression test,and the results show that the ultimate strength of chars decreased at the early stage during the heating process,resulting in a decrease of the char breakage *** the late stage during the heating process,the char breakage energy increased with the increase of heating *** power consumption coefficients CKof different chars were determined by a drum experiment,and then the char degradation behavior under different power consumptions was *** addition,agasification experiment was conducted to determine the gasification activation energy（with CO_2）of lump coal heated for different *** results show that the gasification activation energy increased greatly at the early stage during the heating process,which showed opposite change to the breakage energy of ***,the internal temperature and heat changes of the bonded coal briquette were calculated by using an unsteady heat conduction *** large difference between the surface and the center temperatures of coal and the large amount of heat absorption at the early stage during the heating process may have a negative effect on the breakage energy of chars.

关键词： Melting gasifier Lump coal Degradation Volume hypothesis Heat absorption

EFFECT OF DRILLPIPE ROTATION ON BOREHOLE CLEANING FOR EXTENDED REACH WELL

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Journal of Hydrodynamics 2009年第3期21卷 366-372页

作者： WANG Zhi-ming GUO xiao-le li ming HONG Yu-kui College of Oil and Gas Engineering China University of Petroleum Beijing 102249 China

Wellbore cleaning is a key technology for the Extended Reach Drilling （ERD）. The success of drilling extended reach wells depends directly upon the quality of wellbore cleaning. The rotation of the drillpipe, and hydraulic and rheological parameters, are the key factors for wellbore cleaning. In this study, Computational Fluid Dynamics （CFD） is applied to simulate solid-liquid two-phase flows in wellbore annulus. The annual flow field with the presence of drillpipe rotation is described through analyzing various rotation conditions. The results indicate that the quasi-spiral flow is the main flow pattern for liquid-solid transport in the horizontal annulus. The influence of rotation on the cuttings transport is also investigated, as the rotation of drillpipe increases the disturbance of liquid to solid in annulus. As a result, the solid flow becomes favorable and the solid volume is reduced. Comparisons with previously published results are also performed to prove the importance of drillpipe rotation in the wellbore clearing for the ERD.

关键词： Extended Reach Drilling (ERD) borehole cleaning solid-liquid two-phase flow drillpipe rotation simulation spiral flow

Desulfurization thermodynamics experiment of stainless steel pickling sludge

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Journal of Iron and Steel Research International 2019年第5期26卷 519-528页

作者： xiao-ming li ming Lv Wei-dong Yin Jun-xue Zhao Ya-ru Cui School of Metallurgical Engineering Xi'an University of Architecture and Technology Xi'an 710055 Shaanxi China Research Center of Metallurgical Engineering and Technology of Shaanxi Province Xi'an University of Architecture and Technology Xi'an 710055 Shaanxi China

A technical idea of returning stainless steel pickling sludge to smelting process so as to recycle valuable metals of Fe, Cr, and Ni was proposed, expecting to realize sludge detoxification and resource utilization. Thermodynamics calculation showed that not only most valuable elements including Fe, Cr and Ni, but also the harmful element of sulfur in pickling sludge can be enriched into molten metal. As a result, a theoretical study on desulfurization thermodynamics of sludge pretreatment was conducted, and results indicated that complete desulfurization temperature of pure pickling sludge was above 1400℃. When 0.5-0.8% carbon was added as reducing agent, the complete desulfurization temperature was reduced to 1000℃ or below. Desulfurization roasting experiment indicated that desulfurization rate at 1400℃ under inert environment was 78.74% and maximum desulfurization rate at 1000℃ under reducing environment was 91.62%.

关键词： Stainless steel Pickling sludge Pre-treatment Desulfurization thermodynamics

Pan-Arctic ocean wind and wave data by spaceborne SAR

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Big Earth Data 2022年第2期6卷 144-163页

作者： xiao-ming li Ke Wu Bingqing Huang Key Laboratory of Digital Earth Science Aerospace Information Research InstituteChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina

The Arctic is one of the most significant changing areas on the Earth under the climate change *** regions in the Arctic are becoming ice-free oceans in the melting season or through the whole ***,ocean wind and wave,as the two most important parameters in the air-sea interface,are drawing signifi-cant attention to the Arctic *** and radar alti-meter are the two traditional remote sensing instruments for ocean wind and wave observations,while the former is limited by coarse spatial resolution and the latter has small spatial *** and wave data in high spatial resolution and wide coverage by synthetic aperture radar(SAR)are currently lacking in the Arctic *** developed an ocean wind and wave dataset by Sentinel-1 SAR in the pan-Arctic Ocean(above 60°N),covering January 2017 to May *** comparing with sea surface wind speed data of scatterometer,the SAR-retrieved wind data achieve an accuracy of 1.23 m/s,in terms of root mean square error(RMSE).Compared with significant wave height data of radar altimeter,the SAR retrievals have an RMSE of 0.66 *** data records are in the standard NetCDF-4 *** dataset is publicly available at:http://***/10.11922/sciencedb.00834.

关键词： Arctic synthetic aperture radar(SAR) ocean wind ocean wave

Probabilistic Teleportation of an Unknown One-Particle State by a Three-Particle General W State

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Communications in Theoretical Physics 2007年第4期47卷 625-628页

作者： XIU xiao-ming DONG li GAO Ya-Jun Department of Physics Bohai University Jinzhou 121000 China

Two schemes for teleporting an unknown one-particle state are proposed when a general W state is utilized as quantum channel. In the first scheme, after the sender （Alice） makes a Bell-state measurement on her particles, the recipient （Bob） performs a Von Neumann measurement and introduces an auxiliary particle, and carries out a unitary transformation on his particle and the auxiliary particle, and performs a Von Neumann measurement on the auxiliary particle to confirm whether the teleportation succeeds or not. In the second scheme, the recipient （Bob） does not need to perform the first Von Neumann measurement or introduce the auxiliary particle, which is necessary in the first scheme. It is shown that the maxima/probabilities of successful teleportation of the two schemes are identical if the recipient （Bob） performs an appropriate unitary transformation and adopts a proper particle on which he recovers the quantum information of state to be teleported.

关键词： quantum information probabilistic teleportation entanglement general W state