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Molecular characterization of two novel esterase genes from carmine spider mite, Tetranychus cinnabarinus （Acarina： Tetranychidae）

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Insect Science 2010年第2期17卷 91-100页

作者： Wei Sun Chuan-hua Xue Lin He Wen-Cai Lu ming Li Xiao-Fang cao Zhi-Mo Zhao Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing China

Two novel esterase complementary DNAs were identified and cloned from the insecticide-susceptible strain of Tetranychus cinnabarinus （Boisduval）（Acarina： Tetrany- chidae）, which were designated as TCE1 and TCE2, respectively. The cDNA of TCE1 gene contained an open reading frame （ORF） of 1701 bp encoding 567 amino acids, and a predicted molecular weight of 62.75 kDa, the cDNA of TCE2 contained an ORF of 1680 bp encoding 560 amino acids, and a predicted molecular weight of 63.14 kDa. TCE1 and TCE2 were submitted to GenBank, accession number EU 130461 and EU 130462. The well-conserved sequence motif, GXSXG, used as a signature pattern in the esterase fam- ily are present in both TCE1 and TCE2 （GQSAG in TCE1, whereas GESAG in TCE2）, indicating that these two genes are predicted to be esterases. Comparison of the deduced amino acid sequence with the published mite esterase sequence coming from Boophilus microplus showed that TCE1 shares 33.98% identity and TCE2 shares 33.46% identity. TCE1 and TCE2 share 46.4% identity. Quantitative real-time polymerase chain reaction revealed that expression level of the TCE2 gene was relatively higher than that of the TCE1 in all instars examined except the protonymph, and the expression level of these two esterase genes in adults of T. cinnabarinus was significantly higher than that in any other instars, respectively. T. cinnabarinus is an important agricultural mite pest and esterases are important in the metabolisms of insects and mites; the genomic information obtained in this study will contribute to esterase molecular biological study on mite pest species.

关键词： esterase gene molecular cloning quantitative real-time PCR Tetranychus cinnabarinus

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Pitting corrosion and crevice corrosion behaviors of high nitrogen austenitic stainless steels

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International Journal of Minerals,Metallurgy and Materials 2009年第5期16卷 517-524页

作者： hua-bing Li Zhou-hua Jiang Yan Yang Yang cao Zu-rui Zhang School of Materials and Metallurgy Northeastern University Shenyang 110004 China

Pitting corrosion and crevice corrosion behaviors of high nitrogen austenitic stainless steels （HNSS） were investigated by electrochemical and immersion testing methods in chloride solution, respectively. The chemical constitution and composition in the depth of passive films formed on HNSS were analyzed by X-ray photoelectron spectrum （XPS）. HNSS has excellent pitting and crevice corrosion resistance compared to 316L stainless steel. With increasing the nitrogen content in steels, pitting potentials and critical pitting temperature （CPT） increase, and the maximum, average pit depths and average weight loss decrease. The CPT of HNSS is correlated with the alloying element content through the measure of alloying for resistance to corrosion （MARC）. The MARC can be expressed as an equation of CPT=2.55MARC-29. XPS results show that HNSS exhibiting excellent corrosion resistance is attributed to the enrichment of nitrogen on the surface of passive films, which forms ammonium ions increasing the local pH value and facilitating repassivation, and the synergistic effects of molybdenum and nitrogen.

关键词： high nitrogen austenitic stainless steel pitting corrosion crevice corrosion nitrogen critical pitting temperature syner-gistic effect

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Circular dichroism of graphene oxide： the chiral structure model

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Frontiers of Materials Science 2013年第1期7卷 83-90页

作者： Jing cao hua-Jie YIN Rui SONG College of Chemistry and Chemical Engineering University of Chinese Academy of Sciences Beijing 100049 China Laboratory of Nanomaterials National Center for Nanoscience and Technology Beijing 100190 China

We have observed the circular dichroism signal of dilute graphene oxide (GO), then systematically investigated the chirality of GO and established a probable chiral unit model. This study may open up a new field for u... 详细信息

关键词： graphene oxide (GO) circular dichroism XPS chiral model

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A free monoid containing all prefix codes

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Applied Mathematics(A Journal of Chinese Universities) 2024年第4期39卷 596-603页

作者： cao Chun-hua LIU Qin YANG Di School of Mathematics and Statistics Yunnan UniversityKunming 650405China Chongqing Tongnan Experimental Middle School Chongqing 402660China Zhonghua Vocational College Yunnan University of Finance and EconomicsKunming 650221China

In this paper,we exhibit a free monoid containing all prefix codes in connection with the sets of i-th powers of primitive words for all i≥*** extends two results given by Shyr and Tsai in 1998 at the same time.

关键词： prefix code free monoid primitive word maximal prefix code

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Experimental research on charging characteristics of a solar photovoltaic system by the pressure-control method

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第6期12卷 470-474页

作者： hua ZHU Zhang-lu XU Zi-juan cao Institute of Thermal Science and Power Systems Zhejiang University Hangzhou 310027 China

The charging characteristics of the valve-regulated lead acid(VRLA) battery driven by solar energy were experimentally studied through the pressure-control method in this *** aims of the research were to increase charging efficiency to make the most of solar energy and to improve charging quality to prolong life of *** charging process of a 12 V 12 A·h VRLA battery has been tested under the mode of a stand-alone photovoltaic(PV) *** show that the pressure-control method can effectively control PV charging of the VRLA battery and make the best of PV cells through the maximum power point tracking(MPPT).The damage of VRLA battery by excess oxygen accumulation can be avoided through the inner pressure control of VRLA *** such as solar radiation intensity,charging power,inner pressure of the battery,and charging current and voltage during the charging process were measured and analyzed.

关键词： Solar photovoltaic(PV) system Charging characteristics Pressure-control method

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Intergranular corrosion behavior of high nitrogen austenitic stainless steel

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International Journal of Minerals,Metallurgy and Materials 2009年第6期16卷 654-660页

作者： hua-bing Li Zhou-hua Jiang Zu-rui Zhang Yang cao Yan Yang School of Materials and Metallurgy Northeastern University Shenyang 110004 China

The intergranular corrosion （IGC） behavior of high nitrogen austenitic stainless steel （HNSS） sensitization treated at 650-950℃ was investigated by the double loop electrochemical potentiodynamic reactivation （DL-EPR） method. The effects of the electrolytes, scan rate, sensitizing temperature on the susceptibility to IGC of HNSS were examined. The results show that the addi-tion of NaCl is an effective way to improve the formation of the cracking of a passive film in chromium-depleted zones during the reactivation scan. Decreasing the scan rate exhibits an obvious effect on the breakdown of the passive film. A solution with 2 mol/L H2SO4＋1 mol/L NaCl＋0.01 mol/L KSCN is suitable to check the susceptibility to IGC of HNSS at a sensitizing temperature of 650-950℃ at a suitable scan rate of 1.667 mV/s. Chromium depletion of HNSS is attributed to the precipitation of Cr2N which results in the susceptibility to IGC. The synergistic effect of Mo and N is suggested to play an important role in stabilizing the passive film to prevent the attack of IGC.

关键词： intergranular corrosion high nitrogen austenitic stainless steel sensitization passive film chromium depletion

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Effects of current path on structure and segregation during electroslag remelting process of Inconel 718 alloy with same power input

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Journal of Iron and Steel Research International 2021年第12期28卷 1574-1581页

作者： Fu-bin Liu Hai-bo cao hua-bing Li Zhou-hua Jiang Xin Geng School of Metallurgy Northeastern UniversityShenyang110819LiaoningChina Key Laboratory of Neutronics and Radiation Safety Institute of Nuclear Energy Safety TechnologyChinese Academy of SciencesHefei230031AnhuiChina State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang110819LiaoningChina

Current-conductive mold was recently developed to extend electroslag remelting(ESR)functions to overcome some solidification defects by changing the current *** macrostructures,microstructures,macrosegregation,and microsegregation of the Inconel 718 ingots produced by the custom laboratory-scale ESR furnace under different current paths(the classical ESR and the single power,and two circuits ESR process with current-conductive mold(ESR-STCCM))with the same power input were compared and *** results indicate that when the ingot was produced during ESR and ESR-STCCM processes,at the same power input,the pool depth was 104 and 90 mm,respectively.A flatter and shallower molten pool was obtained during ESR-STCCM ***,compared with a classical ESR ingot,the cooling rate of the centerline of ESR-STCCM ingot was increased from 12.7 to 16.7 K min^(−1).The increased cooling rates caused by decreased melting rate and thinner slag skin reduced the growth angle of columnar crystal to the vertical axis and the secondary dendrite arm ***,the macrosegregation and microsegregation of segregation elements for ESR-STCCM process were dramatically reduced compared with ESR *** average volume fraction of Laves phase was reduced from 7.39%to 6.14%,and the segregation of Nb in Laves phase was significantly reduced.

关键词： Electroslag remelting Current path Structure Segregation Inconel 718 alloy Current-conductive mold

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A two-step discriminated method to identify thermophilic proteins

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International Journal of Biomathematics 2017年第4期10卷 123-130页

作者： hua Tang Ren-Zhi cao Wen Wangi Tie-Shan Liu Li-ming Wang Chun-Mei He Department of Pathophysiology Southwest Medical University Luzhou 646000 P. R. China Computer Science Department Pacific Lutheran University Tacoma WA 98447 USA Maize Institute Shandong Academy of Agricultural Science Jinan 250100 P. R. China

Improving thermostability of an enzyme can accelerate the relevant chemical reaction. Thus, the analysis and prediction of thermophilic proteins are conducive to protein engineering and enzyme design. In this study, a novel method based on two-step discrimination was proposed to distinguish between thermophilic and non-thermophilic proteins. The model was rigorously benchmarked on an objective dataset including 915 thermophilic proteins and 793 non-thermophilic proteins. Results showed that the over- all accuracy of our method is 94.44% in 5-fold cross-validation, which is higher than those of other published methods. We believe that the two-step discriminated strategy will become a promising method in the relevant field of protein bioinformatics.

关键词： Thermostability thermophilic protein fragment support vector machine.

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Performance Modeling for Data Monitoring Services in Smart Grid: A Network Calculus Based Approach

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CSEE Journal of Power and Energy Systems 2020年第3期6卷 610-618页

作者： Junwei cao Yuxin Wan Haochen hua Gang Yang Beijing National Research Center for Information Science and Technology Tsinghua UniversityBeijing 100084China College of Energy and Electrical Engineering Hohai UniversityNanjing 211100China

This paper focuses on solving the modeling issues of monitoring system service performance based on the network calculus ***,we formulate the service model of the smart grid monitoring ***,we derive the flow arrival curve based on the incremental process related ***,we develop flow arrival curves for the case of the incremental process being a fractional Gaussian process,and then we obtain the generalized Cauchy *** technical theorems related to network calculus are presented as our main ***,the variance of arrival flow for the continuous time case is *** that the incremental process of network flow is a Gaussian stationary process,and given the auto-correlation function of the incremental process with violation probability,the formula of the arrival curve is *** addition,the overall flow variance under the discrete time case is explicitly *** theoretical results are evaluated in smart grid *** indicate that the generalized Cauchy process outperforms the fractional Gaussian process for our considered problem.

关键词： Fractional Gaussian process generalized Cauchy process monitoring systems network calculus service performance

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LS-SVM model based nonlinear predictive control for MCFC system

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第5期8卷 748-754页

作者： CHEN Yue-hua cao Guang-yi ZHU Xin-jian Institute of Fuel Cell Department of Automation Shanghai Jiao Tong University Shanghai 200030 China

This paper describes a nonlinear model predictive controller for regulating a molten carbonate fuel cell (MCFC). In order to improve MCFC’s generating performance, prolong its life and guarantee safety, it must be controlled efficiently. First, the output voltage of an MCFC stack is identified by a least squares support vector machine (LS-SVM) method with radial basis function (RBF) kernel so as to implement nonlinear predictive control. And then, the optimal control sequences are obtained by applying genetic algorithm (GA). The model and controller have been realized in the MATLAB environment. Simulation results indicated that the proposed controller exhibits satisfying control effect.

关键词： Molten carbonate fuel cell (MCFC) Least squares support vector machine (LS-SVM) Genetic algorithm (GA) Nonlinear predictive controller

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