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Clustered spikelets 4,encoding a putative cytochrome P450 protein CYP724B1,is essential for rice panicle development

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Chinese Science Bulletin 2014年第31期59卷 4050-4059页

作者： Min guo Yi-Hao Yang Min Liu Qing-Cai Meng Xiu-hong Zeng Ling-Xia Dong Shu-Zhu Tang ming-hong gu Chang-Jie Yan Jiangsu Key Laboratory of Crop Genetics and Physiology/ Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou University

Rice(Oryza sativa L.)inflorescence(panicle)architecture is an important agronomic trait,serving as one of the determinants of rice yield.A number of genes related to panicle development have been cloned and functionally characterized so ***,more information is needed for fully understanding of the mechanism underlying the panicle *** the present study,we identified a clustered spikelets 4(cl4)mutant in the 93-11 genetic *** to its wild-type 93-11,cl4 mutant has a typical clustered spikelets phenotype with all primary branches clustered on the base of the main rachis and 2–3abnormal spikelets clustered on the primary ***,cl4 mutant also shows shorter plant height than that of the wild ***-based cloning strategy is performed to clone the CL4 *** a result,CL4 is demonstrated to encode a putative cytochrome P450 protein CYP724B1,which is involved in brassinosteroid *** confirm our mapping result,the CL4 RNAi transgenic plants are *** the transgenic plants also show similar phenotype as the cl4 *** results provide strong evidence that CL4 plays an important role in rice panicle development as well as plant height regulation.

关键词：细胞色素P450 水稻产量小穗集群蛋白编码稻穗转基因植物

Rice OsGL 1-1 Is Involved in Leaf Cuticular Wax and Cuticle Membrane

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Molecular Plant 2011年第6期4卷 985-995页

作者： Bao-Xiang Qin Ding Tang Jian Huang ming Li Xin-Ru Wu Li-Li LU Ke-Jian Wang Heng-Xiu Yu Jian-Min Chen ming-hong gu Zhu-Kuan Cheng State Key Laboratory of Plant Genomics and Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Key Laboratory of Plant Functional Genomics of Ministry of Education Yangzhou University Yangzhou Jiangsu 225009 China

Cuticular wax forms a hydrophobic barrier on aerial plant organs; it plays an important role in protecting a plant from damage caused by many forms of environmental stress. In the present study, we characterized a rice leaf wax-deficient mutant osgll-1 derived from a spontaneous mutation, which exhibited a wax-deficient and highly hydro- philic leaf phenotype. We cloned the OsGLI-1 gene by the map-based cloning method and performed a complementation test to confirm the function of the candidate gene. Molecular studies revealed that OsGLI-1 was a member of the OsGL1 family, and contained regions that were homologous to some regions in sterol desaturases and short-chain dehydro- genases/reductases. Compared to the wild-type, the osgll-1 mutant showed decreased cuticular wax deposition, thinner cuticular membrane, decreased chlorophyll leaching, increased rate of water loss, and enhanced sensitivity to drought. OsGL 1-1 is expressed ubiquitously in rice. The transient expression of OsGLl-l-green fluorescent protein fusion protein indicated that OsGLI-1 is localized in the cytoplasm, plasma membrane, and nucleus.

关键词： Cuticular wax cuticular membrane OsGL1-1 rice.

Development and high-throughput genotyping of substitution lines carting the chromosome segments of indica 9311 in the background of japonica Nipponbare

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Journal of Genetics and Genomics 2011年第12期38卷 603-611页

作者： Hua Zhang Qiang Zhao Zhi-Zhong Sun Chang-Quan Zhang Qi Feng Shu-Zhu Tang guo-Hua Liang ming-hong gu Bin Han Qiao-Quan Liu Jiangsu Key Laboratory of Crop Genetics and Physiology~Key Laboratory of the Ministry of Education for Plant Functional Genomics College of Agriculture Yangzhou University Yangzhou 225009 China National Center for Gene Research Institute of Plant Physiology and Ecology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 200233 China National Center for Plant Gene Research （Shanghai） Shanghai 200032 China

Chromosome segment substitution lines（CSSLs） are useful for the precise mapping of quantitative trait loci（QTLs） and dissection of the genetic basis of complex *** this study,two whole-genome sequenced rice cultivars,the japonica Nipponbare and indica 9311 were used as recipient and donor,respectively.A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing *** graphical genotypes of 38 lines were constructed based on resequencing *** CSSLs had a total of 95 substituted segments derived from indica 9311,with an average of about 2.5 segments per CSSL and eight segments per chromosome,and covered about 87.4%of the rice whole genome.A multiple linear regression QTL analysis mapped four QTLs for 1000-grain *** largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in *** CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome

关键词： Oryza sativa L. Chromosome segment substitution lines(CSSLs) Molecular marker-assisted selection High-throughput resequencing

Molecular Cytogenetic Analysis of Spontaneous Interspecific Hybrid Between Oryza sativa and Oryza minuta

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Rice science 2008年第4期15卷 283-288页

作者： Chuan-deng YI Xu CHENG Bei-bei WANG guo-hua LIANG Zhi-yun GONG Shu-zhu TANG ming-hong gu Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics Ministry of Education Yangzhou University Yangzhou 225009 China

Genomic in situ hybridization (GISH) is a powerful tool to characterize parental chromosomes in interspecific hybrids, including the behaviour of autosynapsis and chromosome pairing. It was used to distinguish the chromosomes of Oryza sativa from wild species in a spontaneous interspecific hybrid and to investigate the chromosome pairing at metaphase I in meiosis of the hybrid in this study. The hybrid was a triploid with 36 chromosomes according to the chromosome number investigated in mitosis of root tips. During metaphase I of meiosis in the hybrid, less chromosome pairing was observed and most of the chromosomes existed as univalent. Based on GISH and FISH (Fluorescent in situ hybridization) analyses, the chromosomes of the hybrid were composed of genomes A, B and C. Thus, it was believed that the hybrid was the result of natural hybridization between cultivated rice and wild species O. minuta which was planted in experimental fields.

关键词： wild rice interspecific hybrid genomic in situ hybridization fluorescent in situ hybridization molecular cytogenetics meiosis chromosome pairing genome

Cytological identification of an isotetrasomic in rice and its application to centromere mapping

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Cell Research 1997年第1期7卷 31-38页

作者： CHENG ZHU KUAN HENG XIU YU CHANG JIE YAN LI HUANG ZHU ming hong gu(Department of Agronomy, Agriculture College, Yangzhou University, Yangzhou 225009)(Institute of Genetics, Chinese Academy of Sciences,Beijing 100101) Department of Agronomy Agriculture College Yangzhou University Yangzhou Institute of Genetics Chinese Academy of Sciences Beijing

The aneuploid with isochromosome or telochromosome is ideal material for exploring the position of centromere in lingkage map. FOr obtaining these aneuploids in rice, the primary trisomics from triplo-1 to triplo-12 and the aneuploids derived from a triploid of indica rice variety Zhongxian 3037 were carefully investigated. From the offsprings of triplo-10, a primary trisomic of chromosome 10 of the variety, an isotetrasomic "triplo-10-1" was obtained. Cytological investigation revealed that a pair of extra isochromosomes of triplo-10-1 were come from the short arm of chromosome 10. In the offsprings of the isotetrasomic, a secondary trisomic "triplo-10-2" 5 in which the extra- chromosome was an isochromosome derived from the short arm of chromosome 10, was identified. With the isotetrasomic, secondary trisomic, primary trisomic and diploid of variety Zhongxian 3037, different molecular markers were used for exploring the position of the centromere of chromosome 10. Based on the DNA dosage effect, it was verified that the molecular markers G1125, G333 and L169 were located on the short arm, G1084 and other 16 available molecular markers were on the long arm of chromosome 10. So the centromere of chromosome 10 was located somewhere betweenG1125 and G1084 according to the RFLP linkage map given by Kurata et al[1]. The distance from G1125 to G1084 was about 3.2cM.

关键词： Rice isotetrasomic centromere mapping

GPS: graph contrastive learning via multi-scale augmented views from adversarial pooling

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Science China(Information Sciences) 2025年第1期68卷 145-158页

作者： Wei JU Yiyang gu Zhengyang MAO Ziyue QIAO Yifang QIN Xiao LUO Hui XIONG ming ZHANG School of Computer Science National Key Laboratory for Multimedia Information ProcessingPeking University Artificial Intelligence Thrust The Hong Kong University of Science and Technology Department of Computer Science University of California

Self-supervised graph representation learning has recently shown considerable promise in a range of fields, including bioinformatics and social networks. A large number of graph contrastive learning approaches have shown promising performance for representation learning on graphs, which train models by maximizing agreement between original graphs and their augmented views(i.e., positive views). Unfortunately, these methods usually involve pre-defined augmentation strategies based on the knowledge of human experts. Moreover, these strategies may fail to generate challenging positive views to provide sufficient supervision signals. In this paper, we present a novel approach named graph pooling contrast(GPS) to address these *** by the fact that graph pooling can adaptively coarsen the graph with the removal of redundancy, we rethink graph pooling and leverage it to automatically generate multi-scale positive views with varying emphasis on providing challenging positives and preserving semantics, i.e., strongly-augmented view and weakly-augmented view. Then, we incorporate both views into a joint contrastive learning framework with similarity learning and consistency learning, where our pooling module is adversarially trained with respect to the encoder for adversarial robustness. Experiments on twelve datasets on both graph classification and transfer learning tasks verify the superiority of the proposed method over its counterparts.

关键词： graph representation learning graph neural networks graph contrastive learning graph augmentations graph pooling

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钛酸钾晶须表面处理及对聚烯烃增强

上海交通大学学报 2004年第10期38卷 1677-1681页

作者：郭明波徐宏古宏晨上海交通大学纳米科学与技术工程中心上海200030

为改善聚烯烃(PO)体系强度,对钛酸钾晶须(K2Ti6O13,PTW)进行表面无机包硅处理,并比较了硅烷偶联剂改性前后晶须对PO增强性能的改变.实验结果表明,包硅处理后经硅烷偶联剂KH-570表面改性的PTW能与PO基体更好地相容,达到较好的增强效果,... 详细信息

为改善聚烯烃(PO)体系强度,对钛酸钾晶须(K2Ti6O13,PTW)进行表面无机包硅处理,并比较了硅烷偶联剂改性前后晶须对PO增强性能的改变.实验结果表明,包硅处理后经硅烷偶联剂KH-570表面改性的PTW能与PO基体更好地相容,达到较好的增强效果,在添加量10%的情况下,PO体系的冲击强度略有下降,但拉伸强度提高40%.

关键词：钛酸钾晶须聚烯烃硅烷偶联剂表面处理

Multilevel analysis of the central-peripheral-target organ pathway:contributing to recovery after peripheral nerve injury

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Neural Regeneration Research 2025年第10期20卷 2807-2822页

作者： Xizi Song Ruixin Li Xiaolei Chu Qi Li Ruihua Li Qingwen Li Kai-Yu Tong Xiaosong gu Dong ming Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Haihe Laboratory of Brain-Machine Interface and Human-Machine Fusion TianjinChina Department of Rehabilitation Tianjin University Tianjin HospitalTianjinChina Department of Hand Microsurgery Tianjin University Tianjin HospitalTianjinChina School of Exercise and Health Tianjin University of SportTianjinChina Department of Biomedical Engineering the Chinese University of Hong KongHong Kong Special Administrative RegionChina

Peripheral nerve injury is a common neurological condition that often leads to severe functional limitations and *** on the pathogenesis of peripheral nerve injury has focused on pathological changes at individual injury sites,neglecting multilevel pathological analysis of the overall nervous system and target *** has led to restrictions on current therapeutic *** this paper,we first summarize the potential mechanisms of peripheral nerve injury from a holistic perspective,covering the central nervous system,peripheral nervous system,and target *** peripheral nerve injury,the cortical plasticity of the brain is altered due to damage to and regeneration of peripheral nerves;changes such as neuronal apoptosis and axonal demyelination occur in the spinal *** nerve will undergo axonal regeneration,activation of Schwann cells,inflammatory response,and vascular system regeneration at the injury *** damage to target organs can occur,including skeletal muscle atrophy and sensory receptor *** then provide a brief review of the research advances in therapeutic approaches to peripheral nerve *** main current treatments are conducted passively and include physical factor rehabilitation,pharmacological treatments,cell-based therapies,and physical ***,most treatments only partially address the problem and cannot complete the systematic recovery of the entire central nervous system-peripheral nervous system-target organ ***,we should further explore multilevel treatment options that produce effective,long-lasting results,perhaps requiring a combination of passive(traditional)and active(novel)treatment methods to stimulate rehabilitation at the central-peripheral-target organ levels to achieve better functional recovery.

关键词： central nervous system central peripheral target organ multilevel pathological analysis nerve regeneration peripheral nerve injury peripheral nervous system target organs therapeutic approach

Breaking new ground in photocatalysis: lutetium texaphyrin as a trigger for pyroptosis through biomolecular photoredox reactions

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Science China Chemistry 2025年第1期68卷 1-2页

作者： Qihang Ding Mengrong He Meijia gu Department of Chemistry Korea University Department of Ultrasound Medicine Union Hospital Tongji Medical College Huazhong University of Science and Technology Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE) School of Pharmaceutical Sciences Wuhan University

Malignant neoplasms consistently pose a significant threat to global public health [1,2]. In recent years, immunotherapy has demonstrated remarkable clinical success; however, its efficacy is limited by the scarcity of immune cell infiltration within tumor microenvironments. Cell pyroptosis, a gasdermin-mediated process characterized by cellular swelling,rupture, and the release of intracellular contents such as IL-1β, IL-18, ATP, HMGB1, plays a crucial role in this context[3,4].

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