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Mechanistic target of rapamycin kinase(Mtor)is required for spermatogonial proliferation and differentiation in mice

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Asian Journal of Andrology 2020年第2期22卷 169-176页

作者： Jun Cao Zuo-Bao Lin ming-han tong Yong-Lian Zhang Yi-Ping Li Yu-Chuan Zhou State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China

Spermatogonial development is a vital prerequisite for spermatogenesis and male ***,the exact mechanisms underlying the behavior of spermatogonia,including spermatogonial stem cell(SSC)self-renewal and spermatogonial proliferation and differentiation,are not fully *** studies demonstrated that the mTOR complex 1(mTORC1)signaling pathway plays a crucial role in spermatogonial development,but whether MTOR itself was also involved in any specific process of spermatogonial development remained *** this study,we specifically deleted Mtor in male germ cells of mice using Stra8-Cre and assessed its effect on the function of *** Mtor knockout(KO)mice exhibited an age-dependent perturbation of testicular development and progressively lost germ cells and fertility with *** age-related phenotypes were likely caused by a delayed initiation of Mtor deletion driven by *** examination revealed a reduction of differentiating spermatogonia in Mtor KO mice,suggesting that spermatogonial differentiation was *** proliferation was also impaired in Mtor KO mice,leading to a diminished spermatogonial pool and total germ cell *** results show that MTOR plays a pivotal role in male fertility and is required for spermatogonial proliferation and differentiation.

关键词： male fertility mice Mtor spermatogenesis spermatogonia testis

DNA/RNA helicase DHX36 is required for late stages of spermatogenesis

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Journal of Molecular Cell Biology 2022年第11期14卷 50-61页

作者： Kejia Zhang Tianxin Zhang Yujie Zhang Jinyu Yuan Xinzhe Tang Chaobao Zhang Qianqian Yin Yonglian Zhang ming-han tong School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of SciencesHangzhou 310024China State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China

Spermatogenesis is a highly complex developmental process that typically consists of mitosis, meiosis, and spermiogenesis. DNA/RNA helicase DHX36, a unique guanine-quadruplex (G4) resolvase, plays crucial roles in a variety of biological processes. We previously showed that DHX36 is highly expressed in male germ cells with the highest level in zygotene spermatocytes. Here, we deleted Dhx36 in advanced germ cells with Stra8-GFPCre and found that a Dhx36 deficiency in the differentiated spermatogonia leads to meiotic defects and abnormal spermiogenesis. These defects in late stages of spermatogenesis arise from dysregulated transcription of G4-harboring genes, which are required for meiosis. Thus, this study reveals that Dhx36 plays crucial roles in late stages of spermatogenesis.

关键词： Dhx36 meiosis guanine-quadruplex(G4) Spo11 synapsis meiotic recombination crossover

Bilateral carpal tunnel syndrome and motor dysfunction caused by gout and type 2 diabetes:A case report

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World Journal of Clinical Cases 2023年第11期11卷 2535-2540页

作者： Gao-Feng Zhang Cun-Min Rong Wei Li Ben-Lei Wei ming-tong han Qing-Luan han Department of hand and Foot Surgery Affiliated Hospital of Jining Medical UniversityJining 272029Shandong ProvinceChina

BACKGROUND Carpal tunnel syndrome(CTS)has been associated with gout and type 2 diabetes mellitus(T2DM).However,due to insufficient clinical understanding of goutrelated CTS and reliance on the diagnostic importance of elevated serum uric acid levels,such cases are prone to missed diagnosis,misdiagnosis,and delayed *** addition,the effect of T2DM on gout-induced carpal tunnel syndrome has not been *** SUMMARY Herein,we present an unusual case of CTS and motor dysfunction caused by miliary tophaceous gout and *** patient presented to the hand and foot clinic with paresthesia of the fingers of both hands,especially at *** patient was diagnosed with type 2 diabetes a month *** revealed bilateral transverse carpal ligament thickening with median nerve compression during *** patient was successfully treated with carpal tunnel decompression and tendon *** postoperative pathological examination revealed typical gout *** case suggests that the presence of T2DM could accelerate tophi formation and worsen CTS symptoms,although no definitive proof in this regard has been described *** Tophi formation may most likely cause the co-occurrence of CTS and flexor dysfunction in gout and incipient diabetes patients.

关键词： Carpal tunnel syndrome Motor dysfunction Tophaceous gout Type 2 diabetes mellitus Operate Case report

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National Science Review 2015年第4期2卷 384-384页

作者： ming-han tong Jinsong Li Institute of Biochemistry and Shanghai Institues for Biological Sciences Chinese Academy of Sciences

Germ cells have a unique ability to produce a new individual and are paramount for transmiting intact genome and appropriate epigenome from generation to *** germ cells(PGCs),the irst germ cell population established ... 详细信息

关键词： PGCs gene DNA Similarity of epigenetic reprogramming in primordial germ cells between human and mouse

Rescue of male infertility through correcting a genetic mutation causing meiotic arrest in spermatogonial stem cells

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Asian Journal of Andrology 2021年第6期23卷 590-599页

作者： Ying-Hua Wang Meng Yan Xi Zhang Xin-Yu Liu Yi-Fu Ding Chong-Ping Lai ming-han tong Jin-Song Li State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China

Azoospermia patients who carry a monogenetic mutation that causes meiotic arrest may have their biological child through genetic correction in spermatogonial stem cells(SSCs).However,such therapy for infertility has not been experimentally investigated *** this study,a mouse model with an X-linked testis-expressed 11(TEX11)mutation(Tex11PM/Y)identified in azoospermia patients exhibited meiotic arrest due to aberrant chromosome ***11PM/Y SSCs could be isolated and expanded in vitro normally,and the mutation was corrected by clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated endonuclease 9(Cas9),leading to the generation of repaired SSC ***-genome sequencing demonstrated that the mutation rate in repaired SSCs is comparable with that of autonomous mutation in untreated Tex11PM/Y SSCs,and no predicted off-target sites are *** SSCs could restore spermatogenesis in infertile males and give rise to fertile offspring at a high *** summary,our study establishes a paradigm for the treatment of male azoospermia by combining in vitro expansion of SSCs and gene therapy.

关键词： azoospermia gene therapy male infertility meiotic arrest spermatogonial stem cells testis-expressed 11

tongue reconstruction with anterolateral thigh flap for middle-late stage tongue cancer patients

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中国口腔颌面外科杂志 2008年第B5期6卷 203-204页

作者： Liao Gui-qing Su Yu-xiong Liu Hai-chao Wang Qin Ou De- ming Zhang tong-han Department of Oral and Maxillofacial Surgery Guanghua Schoolof Stomatolog% Sun Yat-sen University Guangzhou 510055China

Corrigendum to"Rescue of male infertility through correcting a genetic mutation causing meiotic arrest in spermatogonial stem cells"

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Asian Journal of Andrology 2023年第6期25卷 757-757页

作者： Ying-Hua Wang Meng Yan Xi Zhang Xin-Yu Liu Yi-Fu Ding Chong-Ping Lai ming-han tong Jin-Song Li State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China

There is an error in the second affiliation in the published article by Wang et al.!The correct afiliation for the authors,Xi Zhang and ming-han tong,should be:“2State Key Laboratory of Molecular Biology,Shanghai Key Laboratory of Molecular Andrology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200031,China”.

关键词： Rescue correcting arrest

Multilevel analysis of the central-peripheral-target organ pathway:contributing to recovery after peripheral nerve injury

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Neural Regeneration Research 2025年第10期20卷 2807-2822页

作者： Xizi Song Ruixin Li Xiaolei Chu Qi Li Ruihua Li Qingwen Li Kai-Yu tong Xiaosong Gu Dong ming Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Haihe Laboratory of Brain-Machine Interface and Human-Machine Fusion TianjinChina Department of Rehabilitation Tianjin University Tianjin HospitalTianjinChina Department of hand Microsurgery Tianjin University Tianjin HospitalTianjinChina School of Exercise and Health Tianjin University of SportTianjinChina Department of Biomedical Engineering the Chinese University of Hong KongHong Kong Special Administrative RegionChina

Peripheral nerve injury is a common neurological condition that often leads to severe functional limitations and *** on the pathogenesis of peripheral nerve injury has focused on pathological changes at individual injury sites,neglecting multilevel pathological analysis of the overall nervous system and target *** has led to restrictions on current therapeutic *** this paper,we first summarize the potential mechanisms of peripheral nerve injury from a holistic perspective,covering the central nervous system,peripheral nervous system,and target *** peripheral nerve injury,the cortical plasticity of the brain is altered due to damage to and regeneration of peripheral nerves;changes such as neuronal apoptosis and axonal demyelination occur in the spinal *** nerve will undergo axonal regeneration,activation of Schwann cells,inflammatory response,and vascular system regeneration at the injury *** damage to target organs can occur,including skeletal muscle atrophy and sensory receptor *** then provide a brief review of the research advances in therapeutic approaches to peripheral nerve *** main current treatments are conducted passively and include physical factor rehabilitation,pharmacological treatments,cell-based therapies,and physical ***,most treatments only partially address the problem and cannot complete the systematic recovery of the entire central nervous system-peripheral nervous system-target organ ***,we should further explore multilevel treatment options that produce effective,long-lasting results,perhaps requiring a combination of passive(traditional)and active(novel)treatment methods to stimulate rehabilitation at the central-peripheral-target organ levels to achieve better functional recovery.

关键词： central nervous system central peripheral target organ multilevel pathological analysis nerve regeneration peripheral nerve injury peripheral nervous system target organs therapeutic approach

Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats

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World Journal of Gastroenterology 2025年第1期31卷 93-109页

作者： Ping Zhang Ying Zhu Pu Chen tong Zhou Zhe-Yi han Jun Xiao Jian-Feng Ma Wen Ma Peng Zang Ying Chen Graduate School Hebei North UniversityZhangjiakou 075000Hebei ProvinceChina Key Laboratory of Aerospace Nutrition and Food Engineering China Astronaut Research and Training CenterBeijing 100094China Department of Gastroenterology The Air Force Medical CenterBeijing 100142China Department of Blood Transfusion Air Force Medical CenterBeijing 100142China

BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility *** pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal ***,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal *** cells are the pacemakers of gastrointestinal *** To investigate the effects of Bifidobacterium lactis(***)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit *** The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 *** tail group was given *** BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/***&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and *** fecal flora and mucosal flora of rats in each group were detected by 16S *** Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic *** microgravity reduced the expressions of SCF and c-kit,and increased the express

关键词： Simulated microgravity Rat Intestinal flora Gastrointestinal motility Stem cell factor/c-kit signaling pathway