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Abundance and Diversity of RuBisCO Genes Responsible for CO_2 Fixation in Arid Soils of Northwest China

Pedosphere 2015年第1期25卷 150-159页

作者： TANG Zhi-Xi fan Fen-liang WAN Yun-fan WEI Wei LAI li-ming State Key Laboratory of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Ministry of Agriculture Key Laboratory of Agro-Environment and Climate Change Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural Sciences

Arid soils where water and nutrients are scarce occupy over 30% of the Earth's total surface. However, the microbial autotrophy in the harsh environments remains largely unexplored. In this study, the abundance and diversity of autotrophic bacteria were investigated, by quantifying and profiling the large subunit genes of ribulose-1,5-bisphosphate carboxylase/oxygenase(Ru Bis CO) form I(cbb L) responsible for CO2 fixation, in the arid soils under three typical plant types(Haloxylon ammodendron, Cleistogenes chinensis,and Reaumuria soongorica) in Northwest China. The bacterial communities in the soils were also characterized using the 16 S r RNA gene. Abundance of red-like autotrophic bacteria ranged from 3.94 × 105 to 1.51 × 106 copies g-1dry soil and those of green-like autotrophic bacteria ranged from 1.15 × 106 to 2.08 × 106 copies g-1dry soil. Abundance of both red- and green-like autotrophic bacteria did not significantly differ among the soils under different plant types. The autotrophic bacteria identified with the cbb L gene primer were mainly affiliated with Alphaproteobacteria, Betaproteobacteria and an uncultured bacterial group, which were not detected in the 16 S r RNA library. In addition, 25.9% and 8.1% of the 16 S r RNA genes were affiliated with Cyanobacteria in the soils under H. ammodendron and R. soongorica, respectively. However, no Cyanobacteria-affiliated cbb L genes were detected in the same soils. The results suggested that microbial autotrophic CO2 fixation might be significant in the carbon cycling of arid soils, which warrants further exploration.

关键词： autotrophic bacteria carbon cycling cbb L harsh environments real-time polymerase chain reaction

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基于IWOA-LSTM算法的预应力钢筋混凝土梁损伤识别

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江苏大学学报（自然科学版） 2025年第1期46卷 105-112,119页

作者：范旭红章立栋杨帆李青郁董凯江苏大学土木工程与力学学院江苏镇江212013 河南省公路工程局集团第二公路工程有限公司河南郑州450015 中国葛洲坝集团第二工程有限公司四川成都610091

为准确识别桥梁结构的损伤程度,制作了桥梁的关键构件——预应力钢筋混凝土梁,进行三点弯曲加载试验.收集了损伤破坏全过程的声发射(AE)信号,通过AE信号参数分析,将梁的损伤破坏过程划分为4个典型阶段.构建了长短时记忆神经网络(LSTM)模... 详细信息

为准确识别桥梁结构的损伤程度,制作了桥梁的关键构件——预应力钢筋混凝土梁,进行三点弯曲加载试验.收集了损伤破坏全过程的声发射(AE)信号,通过AE信号参数分析,将梁的损伤破坏过程划分为4个典型阶段.构建了长短时记忆神经网络(LSTM)模型,根据经验设置LSTM模型的超参数容易导致网络陷入局部最优而影响了分类结果,提出采用Sine混沌映射和自适应权重来改进鲸鱼优化算法(WOA),对LSTM进行超参数寻优.设计了IWOA-LSTM算法模型,训练识别试验梁各损伤阶段的AE信号特征参数.定型网络结构,并识别同种工况下其他梁的AE信号.结果表明:IWOA-LSTM算法模型识别准确率均超过或接近92%,相较于普通LSTM模型,IWOA-LSTM模型识别准确率提高了约7%.

关键词：预应力钢筋混凝土梁声发射损伤识别长短时记忆神经网络改进的鲸鱼优化算法

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Complementary doping of van der Waals materials through controlled intercalation for monolithically integrated electronics

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Nano Research 2020年第5期13卷 1369-1375页

作者： ming Ke Huu Duy Nguyen Hang fan Man li Huan Wu Yongjie Hu School of Engineering and Applied Science University of CaliforniaLos AngelesCalifornia 90095USA

Doping control has been a key challenge for electronic applications of van der Waals ***,we demonstrate complementary doping of black phosphorus using controlled ionic intercalation to achieve monolithic building *** characterize the anisotropic electrical transport as a function of ion concentrations and report a widely tunable resistivity up to three orders of magnitude with characteristic concentration dependence corresponding to phase transitions during *** a further step,we develop both p-type and n-type field effect transistors as well as electrical diodes with high device stability and *** addition,enhanced charge mobility from 380 to 820 cm^2/(V·s)with the intercalation process is observed and explained as the suppressed neutral impurity scattering based on our ab initio *** study provides a unique approach to atomically control the electrical properties of van der Waals materials,and may open up new opportunities in developing advanced electronics and physics platforms.

关键词： nanoelectronics two-dimensional(2D)materials and heterostructures FET diode tunable properties black phosphorus

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Hematopoietic effect of small molecular fraction of Polygoni multiflori Radix Praeparata in cyclophosphamide-induced anemia mice

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Chinese Journal of Natural Medicines 2019年第7期17卷 535-544页

作者： ZHU li-li fan Luo-Di HU ming-Hua MA fang-li QI Jin Jiangsu Key Laboratory of TCM Evaluation and Translational Research China Pharmaceutical University Nanjing 210009 China Infinitus Chinese Herbal Immunity Research Centre Infinitus (China) Company Limited. Guangzhou 510663 China

The aim of this study is to investigate the protective effects of a small molecular fraction(SMF) of Polygoni multiflori Radix Praeparata(PMRP) in a cyclophosphamide(CTX) induced anemia mouse model. Small molecular fraction of PMRP was prepared and identified by high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS). In pharmacology, we examined the peripheral hemogram and thymus and spleen index. The content of granulocyte-macrophage colony-stimulating factor(GM-CSF) in serum was mensurated by enzyme-linked immunosorbent assay(EliSA);The level of superoxide dismutase(SOD), catalase(CAT), total antioxidant capacity(T-AOC), and malondialdehyde(MDA) in serum and spleen tissue homogenate were detected, and glutathione peroxidase(GSH-PX) was assayed in spleen. The results show that SMF can significantly accelerate the recovery of peripheral hemogram, increase the activity of antioxidant enzymes and GM-CSF in serum and spleen. SMF also increases the number of spleen cells, improves bone marrow pathology. In conclusion, the SMF of PMRP promoted the recovery of hematopoietic function in a CTX-induced anemia mouse, which can support SMF to be used as an adjunct to chemotherapy to counteract its side effects.

关键词： Polygoni multiflori Radix Praeparata Small molecular fraction Cyclophosphamide Hemopoiesis

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Radiation-induced cross-linking:a novel avenue to permanent 3D modification of polymeric membranes

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Nuclear Science and Techniques 2021年第7期32卷 31-45页

作者： Yu Gu Bo-Wu Zhang Zhen Guo Ji-Hao li ming Yu lin-fan li Jing-Ye li Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China MOE Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials College of Chemistry and Materials ScienceShanghai Normal UniversityShanghai 200234China University of Chinese Academy of Sciences Beijing 100049China

Membrane fouling is always the biggest problem in the practice of membrane separation technologies,which strongly impacts their applicability,separation efficiency,cost effectiveness,and service ***,a simple but effective 3D modification approach was designed for permanently functionalizing polymeric membranes by directly cross-linking polyvinyl alcohol(PVA)under gamma-ray irradiation at room temperature without any *** the modification,a PVA layer was constructed on the membrane surface and the pore inner surface of polyvinylidene fluoride(PVDF)*** endowed them with good hydrophilicity,low adsorption of protein model foulants,and easy recoverability *** addition,the pore size and distribution were customized by controlling the PVA concentration,which enhanced the rejection ability of the resultant membranes and converted them from microfiltration to *** crosslinked PVA layer was equipped with the resultant membranes with good resistance to chemical cleaning by acidic,alkaline,and oxidative reagents,which could greatly prolong the membrane service ***,this approach was demonstrated as a universal method to modify PVDF membranes with other hydrophilic macromolecular modifiers,including polyethylene glycol,sodium alginate,and polyvinyl *** modification of the membranes effectively endowed them with good hydrophilicity and antifouling properties,as expected.

关键词： Ultrafiltration membrane Antifouling 3D modification Gamma-ray Cross-linking

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Turbulent Agglomeration of Microparticles in a Cylinder Wake Flow Using LES-DEM:Focusing on the Effect of the Reynolds Number

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Journal of Thermal Science 2025年第1期 34-49页

作者： WANG Shuang MU lin li Xue XIE Jun DONG ming School of Energy and Power Engineering Dalian University of Technology School of Optical Information and Energy Engineering Wuhan Institute of Technology School of Energy and Environment Shenyang Aerospace University

Turbulent agglomeration is viewed as a promising technology for enhancing fine particle removal *** better understand particle transport,agglomeration behaviors,and fluid-particle interactions,we numerically explored these phenomena under cylindrical vortex wake influence using a coupled large eddy simulation and discrete element method（LES-DEM） *** validity of the LES approach was verified by comparison with available direct numerical simulation（DNS） *** adopted the Johnson-Kendall-Roberts（JKR） contact model for particle-particle *** particle dispersion and agglomeration characteristics of particles with different diameters（dp=2-20 μm） in the laminar and transition of shear layer（TrSL） flow regimes were *** particles were concentrated at the vortex centers,while larger particles accumulated around the *** agglomeration efficiency exhibited an M-shaped profile spanwise（y-direction）.With increasing Reynolds number,the agglomeration efficiency and turbulence intensity *** particle agglomeration efficiency peaks at a certain Reynolds ***,at higher Reynolds numbers,reducing the residence time of particles in the flow field decreases the agglomeration efficiency.

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Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization

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Chinese Chemical Letters 2001年第11期12卷 1007-1010页

作者： li ming DU Zhe Feng fan Jin li QIAO Jing Ping WANG Analysis and Test Center Shanxi Teachers UniversityLingfen 041004 Department of Chemistry Arts and Sciences CollegeTaiyuan University of TechnologyTaiyuan 030006

Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%similar to 95.7% and the RSD is 1.2%similar to4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.

关键词： sparfloxacin HPLC pre-column derivatization human urine

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Bases of the Quantum Cluster Algebra of the Kronecker Quiver

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Acta Mathematica Sinica,English Series 2012年第6期28卷 1169-1178页

作者： ming DING fan XU School of Mathematical Sciences Nankai University Department of Mathematical Sciences Tsinghua University

We construct bar-invariant Z[q ±1/2]-bases of the quantum cluster algebra of Kronecker quiver which are quantum analogues of the canonical basis, semicanonical basis and dual semicanonical basis of the corresponding ... 详细信息

关键词： Quantum cluster algebra,Z[q ±1/2]-basis positivity

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Numerical Simulation of the Stress and Distortion of the Fiberglass-Reinforced Plastics Crust-Stainless Steel liner Compound Conduit

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Journal of Materials Science & Technology 2003年第z1期19卷 69-70页

作者： Hongyuan fanG Chenglei fan Jianguo YANG ming li Zhen LUO Xianzhong HOU State Key Laboratory of Advanced Welding Production Technology Harbin Institute of TechnologyHarbin 150001China School of Material Science and Technology Tianjin UniversityTianjin 30072China Oil-chem-welding Institute Daqing 163712China

A new type of compound conduit is designed and the finite element method(FEM)is applied to analyze the mechanicalperformance of a typical compound *** results of the numerical simulation confirm that the distributionsof the strain,stress and the flexure distortion of the conduit are reasonable under the working conditions,and thestress and strain are continuous at the interface of the crust and the pipe *** conduit can endure 7.4 timesof the working load before break,which fits well with that of the experiment and means that the conduit has goodability to bear *** peak value of the flexure distortion of the conduit(6 m)is about 3 mm in the middlebottom of the pipeline,which also accords well with that of the experiment.

关键词： Compound conduit Stress Distortion Numerical simulation

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Cluster characters for cyclic quivers

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Frontiers of Mathematics in China 2012年第4期7卷 679-693页

作者： ming DING fan XU School of Mathematical Sciences Nankai University Tianjin 300071 China Department of Mathematical Sciences Tsinghua University Beijing 100084 China

We define an analogue of the Caldero-Chapoton map for the cluster category of finite-dimensional nilpotent representations over a cyclic quiver. We prove that it is a cluster character and satisfies some inductive formulas for the multiplication between the generalized cluster variables （the images of objects of the cluster category under this map）. Moreover, we construct a Z-basis for the algebra generated by all generalized cluster variables.

关键词： Cyclic quiver cluster algebra Z-basis

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