检索结果-南通市图书馆

Expression of thymidine kinase mediated by a novel non-viral delivery system under the control of vascular endothelial growth factor receptor 2 promoter selectively kills human umbilical vein endothelial cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第2期14卷 224-230页

作者： Ying Wang Hui-Xiong Xu ming-de lu Qing Tang department of Medical Ultrasonics The First Affiliated Hospital Sun Yat-Sen UniversityGuangzhou 510080 Guangdong Province China department of Hepatobiliary Surgery The First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080Guangdong Province China

AIM: To investigate the killing efficiency of a recombinant plasmid containing a thymidine kinase (TK) domain insert driven by the vascular endothelial growth factor receptor 2 (VEGFR2) promoter (KDR) on vascular endothelial ***: The KDR-TK fragment was extracted from pBluescript Ⅱ KDR-TK plasmid by enzymatic digestion with Xho I and Sal I. The enhanced green fluorescence protein (EGFP) carrier was extracted from pEGFP by the same procedure. The KDR-TK was inserted into the pEGFP carrier to construct pEGFP-KDR-TK. Using ultrasound irradiation and microbubble, pEGFP-KDR-TK was transferred into human umbilical vein endothelial cells (HUVECs). The transient infection rate was estimated by green fluorescent protein (GFP) expression. Transfected HUVECs, non-transfected HUVECs, and HepG2 cells were cultured in the presence of different concentrations of ganciclovir (GCV), and the killing efficacy of HSV-TK/GCV was analyzed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) assay. RESULTS: The recombinant pEGFP-KDR-TK was successfully constructed by inserting the KDR-TK fragment into the pEGFP carrier. Transfected HUVECs showed cytoplasmic green fluorescence, and the transient transfection rate was about 20.3%. Pools of G418-resistant cells exhibited a higher sensitivity to theprodrug/GCV compared to non-transfected HUVECs or non-transfected HepG2 cells, respectively. CONCluSION: KDR promoter and the suicide gene/prodrug system mediated by diagnostic ultrasound combined with microbubble can significantly kill HUVECs. Such therapy may present a novel and attractive approach to target gene therapy on tumor vessels.

关键词： Microbubble Ultrasound Gene therapy Vascular endothelial growth factor receptor 2 Humanumbilical vein endothelial cells

Microbubble-enhanced ultrasound exposure improves gene transfer in vascular endothelial cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第46期12卷 7508-7513页

作者： Fang Nie Hui-Xiong Xu Qing Tang ming-de lu department of Medical Ultrasonics First Affiliated Hospital Sun Yat-Sen UniversityGuangzhou 510080 Guangdong Province China department of Hepatobiliary Surgery First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080Guangdong Province China

AIM： To explore the effects of ultrasound exposure combined with microbubble contrast agent （SonoVue） on the permeability of the cellular membrane and on the expression of plasrnid DNA encoding enhanced green fluorescent protein （pEGFP） transfer into human umbilical vein endothelial cells （HUVECs）. METHODS： HUVECs with fluorescein isothiocyanatedextran （FD500） and HUVECs with pEGFP were exposed to continuous wave （1.9 MHz, 80.0 mW/cm^2） for 5 min, with or without a SonoVue. The percentage of FD500 taken by the HUVECs and the transient expression rate of pEGFP in the HUVECs were examined by fluorescence microscopy and flow cytornetry, respectively. RESULTS： The percentage of FDS00-positive HUVECs in the group of ultrasound exposure combined with SonoVue was significantly higher than that of the group of ultrasound exposure alone （24.0%± 5.5% vs 66.6% ± 4.1%, P 〈 0.001）. Compared with the group of ultrasound exposure alone, the transfection expression rate of pEGFP in HUVECs was markedly increased with the addition of SonoVue （16.1% ± 1.9% vs 1.5% ± 0.2%, P 〈 0.001）. No statistical significant difference was observed in the HUVECs survival rates between the ultrasound group with and without the addition of SonoVue （94.1% ± 2.3% vs 91.1% ± 4.1% ）. CONCluSION： The cell membrane permeability of HUVECs and the transfection efficiency of pEGFP into HUVECs exposed to ultrasound are significantly increased after addition of an ultrasound contrast agent without obvious damage to the survival of HUVECs. This non- invasive gene transfer method may be a useful tool for clinical gene therapy of hepatic tumors.

关键词： Microbubble Ultrasound Gene transfer Human umbilical vein endothelial cell Enhanced green fluorescent protein

Role of contrast-enhanced ultrasound in follow-up assessment after ablation for hepatocellular carcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第6期19卷 855-865页

作者： Shu-Guang Zheng Hui-Xiong Xu ming-de lu Xiao-Yan Xie Zuo-Feng Xu Guang-Jian Liu Lin-Na Liu department of Medical Ultrasound Tenth People's Hospital of Tongji University department of Medical Ultrasonics the First Affiliated HospitalSun Yat-Sen University

AIM:To assess the usefulness of contrast-enhanced ultrasound (CEUS) during follow-up after percutaneous ablation therapy for hepatocellular carcinoma (HCC).METHODS:A total of 141 patients with HCCs who received percutaneous ablation therapy were assessed by paired follow-up CEUS and contrast-enhanced computed tomography (CECT).The follow-up scheme was designed prospectively and the intervals between CEUS and CECT examinations were less than 14 *** im-ages of follow-up CEUS and CECT were reviewed by *** ablated lesions were evaluated and classified as local tumor progression (LTP) and *** was defined as regrowth of tumor inside or adjacent to the successfully treated *** detected new intrahepatic recurrences were also evaluated and defined as presence of intrahepatic new *** CEUS and CECT,LTP and new intrahepatic recurrence both were displayed as typical enhancement pattern of HCC (i.e.,hyper-enhancing during the arterial phase and washout in the late phase).With CECT as the reference standard,the ability of CEUS in detecting LTP or new intrahepatic recurrence during follow-up was ***:During a follow-up period of 1-31 mo (median,4 mo),169 paired CEUS and CECT examinations were carried out for the 141 *** a total of 221 ablated lesions,266 comparisons between CEUS and CECT findings were ***-three LTPs were detected on CEUS whereas 40 LTPs were detected on CECT,there was significant difference (P detected on CEUS were 6 and 13,respectively;the sensitivity,specificity,positive predictive value (PPV),negative predictive value (NPV) and overall accuracy of CEUS in detecting LTPs were 67.5%,97.4%,81.8%,94.4% and 92.3%,***,131 new intrahepatic recurrent foci were detected on CEUS whereas 183 were detected on CECT,there was also significant difference (P < 0.05).In comparison with CECT,the numbers of

关键词： Contrast-enhanced ultrasound Contrastenhanced computed tomography Hepatocellular carcinoma Radiofrequency ablation Microwave ablation

Ultrasound virtual endoscopy: Polyp detection and reliability of measurement in an in vitro study with pig intestine specimens

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第12期22卷 3355-3362页

作者： Jin-Ya Liu Li-Da Chen Hua-Song Cai Jin-Yu Liang ming Xu Yang Huang Wei Li Shi-Ting Feng Xiao-Yan Xie ming-de lu Wei Wang department of Medical Ultrasonics Institute of Diagnostic and Interventional Ultrasound The First Affiliated Hospital of Sun Yat-Sen University department of Radiology The First Affiliated Hospital of Sun Yat-Sen University

AIM: To present our initial experience regarding the feasibility of ultrasound virtual endoscopy(USVE) and its measurement reliability for polyp detection in an in vitro study using pig intestine ***: Six porcine intestine specimens containing 30 synthetic polyps underwent USVE, computed tomography colonography(CTC) and optical colonoscopy(OC) for polyp detection. The polyp measurement defined as the maximum polyp diameter on twodimensional(2D) multiplanar reformatted(MPR) planes was obtained by USVE, and the absolute measurement error was analyzed using the direct measurement as the reference ***: USVE detected 29(96.7%) of 30 polyps, remaining a 7-mm one missed. There was one falsepositive finding. Twenty-six(89.7%) of 29 reconstructedimages were clearly depicted, while 29(96.7%) of 30 polyps were displayed on CTC with one false-negative finding. In OC, all the polyps were detected. The intraclass correlation coefficient was 0.876(95%CI: 0.745-0.940) for measurements obtained with USVE. The pooled absolute measurement errors ± the standard deviations of the depicted polyps with actual sizes ≤ 5 mm, 6-9 mm, and ≥ 10 mm were 1.9 ± 0.8 mm, 0.9 ± 1.2 mm, and 1.0 ± 1.4 mm, ***: USVE is reliable for polyp detection and measurement in in vitro study.

关键词： Three-dimensional ultrasound In vitro Virtual endoscopy Intestinal polyps Technical feasibility

Differentiation of intrahepatic cholangiocarcinoma from hepatocellular carcinoma in high-risk patients: a predictive model using contrast-enhanced ultrasound

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2018年第33期24卷 3786-3798页

作者： Li-Da Chen Si-Min Ruan Jin-Yu Liang Zheng Yang Shun-Li Shen Yang Huang Wei Li Zhu Wang Xiao-Yan Xie ming-de lu ming Kuang Wei Wang department of Medical Ultrasonics Institute of Diagnostic and Interventional Ultrasound The First Affiliated Hospital of Sun Yat-Sen University department of Pathology The Seventh Affiliated Hospital of Sun Yat-Sen University department of Hepatobiliary Surgery The First Affiliated Hospital of Sun Yat-Sen University

AIM To develop a contrast-enhanced ultrasound(CEUS)predictive model for distinguishing intrahepatic cholangiocarcinoma(ICC) from hepatocellular carcinoma(HCC) in high-risk *** This retrospective study consisted of 88 consecutive high-risk patients with ICC and 88 high-risk patients with HCC selected by propensity score matching between May 2004 and July 2016. Patients were assigned to two groups, namely, a training set and validation set, at a 1:1 ratio. A CEUS score for diagnosing ICC was generated based on significant CEUS features. Then, a nomogram based on the CEUS score was developed, integrating the clinical data. The performance of the nomogram was then validated and compared with that of the LR-M of the CEUS Liver Imaging Reporting and Data System(LI-RADS).RESULTS The most useful CEUS features for ICC were as follows: rim enhancement(64.5%), early washout(91.9%), intratumoral vein(58.1%), obscure boundary of intratumoral non-enhanced area(64.5%), and marked washout(61.3%, all P der the curve(AUC) of the CEUS score(AUC = 0.953) for differentiation between ICC and HCC was improved compared to the LI-RADS(AUC = 0.742)(P ded, the CEUS score nomogram was superior to the LI-RADS nomogram(AUC: 0.973 vs 0.916, P = 0.036, Net Reclassification Improvement: 0.077, Integrated Discrimination Index: 0.152). Subgroup analysis demonstrated that the CEUS score model was notably improved compared to the LIRADS in tumors smaller than 5.0 cm(P 0.05).CONCluSION The CEUS predictive model for differentiation between ICC and HCC in high-risk patients had improved discrimination and clinical usefulness compared to the CEUS LIRADS.

关键词： Ultrasonography Hepatocellular carcinoma Intrahepatic cholangiocarcinoma Hepatitis

Diagnosis of liver cirrhosis with contrast-enhanced ultrasound

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Radiology 2010年第1期2卷 32-36页

作者： Guang-Jian Liu ming-de lu Guang-Jian Liu Department of Medical Ultrasonics Institute of Diagnostic and Interventional Ultrasound The First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510080 Guangdong Province China

The assessment of the extent of liver fibrosis is very important for the prognosis and clinical management of chronic liver *** liver biopsy is the gold standard for the assessment of liver fibrosis,new non-invasive diagnostic methods are urgently needed in clinical work due to certain limitations and complications of *** imaging studies play an important role in the diagnosis of focal liver disease and diffuse liver *** them,ultrasonography is the first choice for study of the liver in clinical *** the development of ultrasound contrast agents and contrast specific imaging techniques,contrastenhanced ultrasound(CEUS) shows good performance and great potential in the evaluation of liver *** have tried different kinds of contrast agent and imaging method,such as arrival time of contrast agent in the hepatic vein,and quantitative analysis of the enhancement level of liver parenchyma,to evaluate the degree of liver fibrosis during the past 10 *** review mainly summarizes the clinical studies concerning the assessment of liver fibrosis using CEUS.

关键词： Diagnosis Liver cirrhosis Contrast media Ultrasound

Hepaticojejunostomy for hepatolithiasis: A critical appraisal

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第26期12卷 4170-4174页

作者： Shao-Qiang Li Li-Jian Liang Bao-Gang Peng Jia-ming Lai ming-de lu Dong-ming Li department of Hepatobiliary Surgery the First Affiliated Hospital of Sun Yet-san University Guangzhou 510080 Guangdong Province China

AIM： To evaluate the long-term outcome and surgical indications of hepaticojejunostomy （H J） for the treatment of hepatolithiasis. METHODS- Three hundred and fourteen elective cases with hepatolithiasis but without biliary stricture or cystic dilatation treated in the past 10 years were reviewed retrospectively. The patients were divided into HJ group and T tube drainage group according to biliary drainage procedure. Furthermore, four subgroups were subdivided by hepatectomy as a balance factor, group A1： hepatectomy＋HJ; group A2： choledochoctomy＋HJ; group B1： hepatectomy ＋ choledochoctomy T tube drainage; group B2： choledochoctomy ＋ T tube drainage. The stone residual rate, surgical efficacy and long-term outcome were compared among different procedures. RESULTS： There was no surgical mortality among all patients. The total hospital mortality was 1.6%. The overall stone residual rate after surgical clearance was 25.9%. There was no statistical difference between HJ group and T tube drainage group in terms of stone residual rate after surgical clearance, however, after postoperative choledochoscopic lithotripsy, the total stone residual rate of T tube drainage group was significantly lower than that of HJ group （0.5% vs 16.7%, P 〈 0.01）. Hepatectomy ＋ choledochoctomy tube drainage achieved the optimal therapeutic effect, only 8.2% patients suffered from an attack of cholangitis postoperatively, which was significantly lower than that of hepatectomy ＋ HJ （8.2% vs 22.0%, P = 0.034）. The major reason for postoperative cholangitis was stone residual in the HJ group （16/23, 70.0%）, and stone recurrence in the T tube drainage group （34/35, 97.1%）. The operative times were significantly prolonged in those undergoing HJ, and the operative morbidity of HJ was higher than those of T tube drainage. CONCluSION： The treatment result of HJ for hepatolithiasis is not satisfactory in this retrospective study due to high rate of stone residual and postoperative cholangi- tis. HJ cou

关键词： Hepatolithiasis Hepaticojejunostomy Outcome

Percutaneous ultrasound-guided thermal ablation for liver tumor with artificial pleural effusion or ascites

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer 2010年第9期29卷 830-835页

作者： Lin-Na Liu Hui-Xiong Xu ming-de lu Xiao-Yan Xie department of Medical Ultrasonics the First Affiliated Hospital Sun Yat-sen University Guangzhou Gongdong 510080 P. R. China department of HepatobUiary Surgery the First Affiliated Hospital Sun Yat-sen University Guangzhou Gongdong 510080 P. R. China

Background and Objective:Percutaneous ultrasound-guided thermal ablation is one of the major treatment methods for liver *** location close to the diaphragm or gastrointestinal tract was regarded as the treatment contraindication before due to poor visibility of the tumor or increased risk of thermal injury to the adjacent *** study used artificial pleural effusion or ascites to extend the indications of thermal ablation for liver ***:Artificial pleural effusion (20 cases) or ascites (36 cases) was performed in 56 difficult cases of percutaneous thermal ablation for liver *** technical success rates, the rate of approaching the procedure goal, complications, and local treatment response were ***:The technical success rates were 95%(19/20) for artificial pleural effusion and 100% (36/36) for artificial ascites, the achieve purpose rates were 100%(19/19) and 91.7% (33/36), the complete ablation rates were 84.2% (16/19) and 93.9% (31/33), ***, transient hematuria, and subcutaneous effusion were observed in 3 patients after the procedure of artificial pleural effusion, and hydrothorax in the right chest occurred in 1 patient during the artificial ascites ***:Thermal ablation with the use of artificial pleural effusion or ascites is a safe and effective treatment for liver tumors, and the technique can widen the indications of thermal ablation for liver tumors.

关键词：肝肿瘤人工腹水胸水引导超声局部治疗胸腔积液

评价策略分析框架探索——以英语社论语篇为例

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

外语学刊 2018年第2期 27-34页

作者：孙铭悦张德禄山东大学济南250100 曲阜师范大学曲阜273165 同济大学上海200092

本文力图在前人研究的基础上建立一个评价策略分析框架,以利于在社会交际中选择最适合交际目的的评价资源。根据系统功能语言学的分层模式,在语境层面,文化语境中的体裁和情景语境中的话语基调,通过评价策略中介变量,制约和支配语篇语... 详细信息

本文力图在前人研究的基础上建立一个评价策略分析框架,以利于在社会交际中选择最适合交际目的的评价资源。根据系统功能语言学的分层模式,在语境层面,文化语境中的体裁和情景语境中的话语基调,通过评价策略中介变量,制约和支配语篇语义层面上的评价资源调用;在宏观维度上,决定评价立场的选择,即通过自言或借言识解的主体性价值立场、主体间对话立场和社区性和盟立场,以及对对话扩展或对话压缩的选择;在微观维度上,决定体裁不同阶段的态度、介入和级差的互动模式的选择。立场性和态度性评价依赖介入的人格化和去人格化、态度的铭刻和引发、级差的语力和聚焦等策略。在词汇语法层面,评价策略通过投射结构、情态语法、及物性模式和评价性词汇等机制体现。通过分析可见,该框架有助于揭示语篇的评价意义在语篇中分布和推进的模式,取得与交际目的相关的修辞效果的方式以及由体裁和语域等语境因素促动和支配的方式。

关键词：评价策略评价理论修辞效果