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Function of nonstructural 5A protein of genotype 2a in replication and infection of HCV with gene substitution

World Journal of Gastroenterology 2011年第29期17卷 3398-3406页

作者： Yong-Zhi Wang Wen-Bo Wang ming-mei Cao Wen Wang Lan-Juan Zhao Gang Xu Hao Ren Zhong-Tian Qi Department of Cardiology No.254 Hospital PLATianjin 300142China Department of Microbiology Shanghai Key Laboratory of Medical BiodefenseSecond Military Medical UniversityShanghai 200433China

AIM:To explore the function of Nonstructural 5A(NS5A) protein of genotype 2a(JFH1)in the replication and infection of hepatitis C virus(HCV).METHODS:Intergenotypic chimera FL-J6JFH/J4NS5A was constructed by inserting NS5A gene from 1b stain HC-J4 by the overlapping polymerase chain reaction (PCR)method and the restriction enzyme *** vitro RNA transcripts of chimera,prototype J6JFH and negative control J6JFH1(GND)were prepared and transfected into Huh-7.5 cells with *** assay(IFA),fluorescence quantitative PCR and infection assay were performed to determine the protein expression and gene replication in Huh-7.5 ***:The HCV RNA levels in FL-J6JFH/J4NS5A chimera RNA transfected cells were significantly lower than the wild type at any indicated time point(2.58 ±5.97×106 vs 4.27±1.72×104,P=0.032).The maximal level of HCV RNA in chimera was 5.6±1.8× 104 GE/μg RNA at day 34 after transfection,while the wild type reached a peak level at day 13 which was 126 folds higher(70.65±14.11×105 vs 0.56±0.90 ×105,P=0.028).HCV proteins could also be detected by IFA in chimera-transfected cells with an obviously low *** assay showed that FL-J6JFH/J4NS5A chimera could produce infectious virus particles,ranging from 10±5 ffu/mL to 78.3±23.6 ffu/mL,while that of FL-J6JFH1 ranged from 5.8±1.5×102 ffu/mL to 2.5±1.4×104 ffu/***:JFH1 NS5A might play an important role in the robust replication of *** establishment of FL-J6JFH/J4NS5A provided a useful platform for studying the function of other proteins of HCV.

关键词： Hepatitis C virus Nonstructural 5A Chimera Cell culture-produced virus Replication Infection

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光合作用微生物席主导的寒武系苗岭统崮山组均一石:以山东省泗水县圣水峪剖面为例

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古地理学报 2021年第2期23卷 335-358页

作者：梅冥相中国地质大学(北京)地球科学与资源学院北京100083 中国地质大学(北京)生物地质与环境地质国家重点实验室北京100083

作为一种缺乏沉积构造的隐晶质块状泥晶所表征的非纹层状微生物碳酸盐岩类型,均一石自从命名之后就很少被描述或自古老的地层记录之中被识别出来,再加上缺乏现代实例,造成了对这种微生物碳酸盐岩类型研究的困难。来自于山东省泗水县圣... 详细信息

作为一种缺乏沉积构造的隐晶质块状泥晶所表征的非纹层状微生物碳酸盐岩类型,均一石自从命名之后就很少被描述或自古老的地层记录之中被识别出来,再加上缺乏现代实例,造成了对这种微生物碳酸盐岩类型研究的困难。来自于山东省泗水县圣水峪剖面寒武系苗岭统崮山组中的均一石,以厚层块状、缺乏沉积构造的泥晶灰岩为主,表现为生物层状的微生物礁,与相对较深水的中厚层中至深缓坡相的条带状泥晶灰岩和泥灰岩一起,构成较为典型的潮下型碳酸盐岩米级旋回,集中发育在淹没不整合型三级沉积层序顶部的强迫型海退体系域之中,代表着相对海平面下降阶段的沉积记录。在这些主导着生物层状微生物礁灰岩的均一石中,不均匀地高密度保存着致密缠绕以及较薄的泥晶壁所成型的丝状葛万菌物质体是其最为基本的特征,表明均一石形成于光合作用微生物席复杂的钙化作用。另外,在均一石的致密泥晶组构中,局部发育毫米级至厘米级大小而且形态极端不规则的亮晶显微管状组构。基于其特别的组构特征,将这样的组构解释为具有公共鞘的丝状蓝细菌即念珠菌菌落的钙化作用残余物,其致密泥晶组构可能代表着蓝细菌公共鞘的钙化作用残余物,其中的亮晶显微管则可能为蓝细菌丝状体的钙化作用残余物,而且在这样的组构之中还发育和共生着丝状葛万菌菌落,从而延伸了对蓝细菌菌落钙化作用残余物的认识和解释,并对具这种组构的海绵木乃伊解释提供了另外一种重要的研究镜像。因此,山东省泗水县圣水峪剖面寒武系苗岭统崮山组上部生物层状微生物礁体中的均一石,不仅提供了一个较为典型的寒武纪苗岭世不借助于二氧化碳浓缩作用机制(CCM机制)的蓝细菌钙化作用的实例,而且提供了一个在丝状蓝细菌主导的微生物席之中发育着念珠菌菌落的多重状微生物席的典型实例,从而拓宽了对显生宙最高的大气圈CO2含量以及较高的大气圈O2含量条件下的较为典型的方解石海中与寒武纪后生动物大爆发相平行的蓝细菌繁荣和钙化作用的了解。

关键词：均一石光合作用微生物席崮山组苗岭统寒武系圣水峪剖面山东省华北地台

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光合作用生物膜诱发的放射鲕粒:以江苏徐州贾旺剖面寒武系苗岭统张夏组为例

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古地理学报 2021年第3期23卷 461-488页

作者：梅冥相中国地质大学(北京)地球科学与资源学院北京100083 中国地质大学(北京)生物地质与环境地质国家重点实验室北京100083

几年来针对巴哈马现代文石鲕粒的持续性研究表明,微生物和细胞外聚合物质(EPS)在鲕粒的形成和发育中起着关键而重要的作用,从而产生了一个重要的认识,即:鲕粒可以看作是"纹层状的有机沉积构造"并遵循着微生物岩体系的一些形成特征。但是... 详细信息

几年来针对巴哈马现代文石鲕粒的持续性研究表明,微生物和细胞外聚合物质(EPS)在鲕粒的形成和发育中起着关键而重要的作用,从而产生了一个重要的认识,即:鲕粒可以看作是"纹层状的有机沉积构造"并遵循着微生物岩体系的一些形成特征。但是,鲕粒30亿年的发育历史、多样化的产出环境、特征性的矿物构成和各种各样的沉积组构,确实赋予了鲕粒生长和形成机理的复杂性和神秘性,因为鲕粒在何处而且如何形成、以及鲕粒究竟记录着何种生物与非生物过程的许多问题还存在剧烈争论。来自于江苏徐州贾旺剖面苗岭统张夏组上部鲕粒滩相灰岩,由较为典型的方解石放射鲕粒所组成,表现出放射状、放射—同心状和泥晶质的沉积组构,而且在鲕粒核心、鲕粒皮层以及在鲕粒间的不规则团块或凝块的暗色泥晶质构成中高密度地保存着精美的葛万菌(Girvanella)化石,进一步表明了这些暗色泥晶构成代表着较为特征的光合作用生物膜,从而提供了一个苗岭世方解石海中放射鲕粒形成较为直接的微生物证据,以及与光合作用生物膜之间复杂的成因联系,因为葛万菌是相对较为肯定地类比于近代钙化织线菌(Plectonema)的丝状蓝细菌化石,尽管还可类比于现代的伪枝菌(Scytonema)。虽然形成放射状鲕粒皮层的放射纤维状方解石的沉淀作用确实不能解释为直接的微生物沉淀作用的结果,但是,这些放射鲕粒确实表现出光合作用生物膜诱发、滋养并促进了放射纤维状方解石皮层增生作用的重要证据,为拓宽"鲕粒谜"的阐释提供了一个较为重要的典型实例,而且还成为寒武纪苗岭世方解石海与后生动物辐射相耦合的蓝细菌繁荣的重要证据。

关键词：放射鲕粒光合作用生物膜张夏组寒武系苗岭统徐州贾旺剖面华北地台

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寒武纪丝状蓝细菌生物膜主导和诱发的巨型鲕粒:以河南新安县石井剖面苗岭统徐庄组为例

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古地理学报 2021年第1期23卷 105-124页

作者：梅冥相中国地质大学(北京)地球科学与资源学院北京100083 中国地质大学(北京)生物地质与环境地质国家重点实验室北京100083

多样化的产出环境和30×108年的分布历史表明,鲕粒成因一直是一个谜一样的沉积学难题,争论的关键问题是其究竟是有机(微生物)成因还是无机成因。来自于华北地台寒武系苗岭统徐庄组鲕粒滩相灰岩顶部的方解石巨鲕表现出同心状、放射-同心... 详细信息

多样化的产出环境和30×108年的分布历史表明,鲕粒成因一直是一个谜一样的沉积学难题,争论的关键问题是其究竟是有机(微生物)成因还是无机成因。来自于华北地台寒武系苗岭统徐庄组鲕粒滩相灰岩顶部的方解石巨鲕表现出同心状、放射-同心状和泥晶质的沉积组构,在鲕粒核心和鲕粒皮层中保存着精美的葛万菌(Girvanella)化石所主导的光合作用生物膜的钙化作用残余物,这为研究鲕粒形成与光合作用生物膜之间复杂的成因联系提供了一个较为直接的微生物证据,因为葛万菌可相对较为肯定地类比于近代的钙化织线菌(Plectonema)丝状蓝细菌化石。所以说,在伴随着后生动物辐射的寒武纪蓝细菌繁盛的方解石海中,尽管形成放射状鲕粒皮层的放射纤维状方解石的沉淀作用机理还没有得到完全彻底的了解,但是这些巨鲕确实表现出光合作用生物膜诱发、滋养并促进了鲕粒形成的直接证据,进一步支持了"鲕粒沉积可以作为一种不同的微生物岩体系新类型"的重要科学理念。

关键词：鲕粒光合作用生物膜徐庄组寒武系华北地台

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华北寒武系二级海侵背景下的沉积趋势及层序地层序列:以北京西郊下苇甸剖面为例

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中国地质 2011年第2期38卷 317-337页

作者：梅冥相中国地质大学地球科学与资源学院北京100083

为了达到在地层框架内划分和对比沉积岩的目的,以不整合面为主要地层面,将不整合面及其可以对比的整合面所限定的地层单位定义为沉积层序,并研究沉积层序内的沉积趋势变化,成为今天层序地层学的核心内容。在寒武纪,华北地台接受沉积的... 详细信息

为了达到在地层框架内划分和对比沉积岩的目的,以不整合面为主要地层面,将不整合面及其可以对比的整合面所限定的地层单位定义为沉积层序,并研究沉积层序内的沉积趋势变化,成为今天层序地层学的核心内容。在寒武纪,华北地台接受沉积的时间较晚,所以发育一个不完整的寒武纪地层序列。在该地层序列之中,从混积潮坪沉积到缓坡型碳酸盐台地沉积,组成一个二级海侵背景下的退积序列。在新的寒武系年代地层框架下,以北京西郊寒武系下苇甸剖面为例,重新审视华北地台寒武系层序地层特征,将为研究这一个关键地质历史时期贫乏骨骼的风暴海、随着后生动物辐射而微生物碳酸盐岩也在增加的特殊的沉积作用样式提供重要线索。

关键词：层序地层学寒武系下苇甸剖面北京西郊

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Spontaneous healing after conservative treatment of isolated grade IV pancreatic duct disruption caused by trauma:A case report

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World Journal of Clinical Cases 2022年第18期10卷 6319-6324页

作者： ming-Zhen mei Yu-Feng Ren Yi-Ping Mou Yuan-Yu Wang Wei-Wei Jin Chao Lu Qi-Cong Zhu Departments of General Surgery Tiantai Campus of Zhejiang Provincial People’s HospitalTaizhou 317200Zhejiang ProvinceChina Department of General Surgery Cancer CenterDivision of Gastrointestinal and Pancreatic SurgeryZhejiang Provincial People’s HospitalAffiliated People’s HospitalHangzhou Medical CollegeHangzhou 310014Zhejiang ProvinceChina Key Laboratory of Gastroenterology of Zhejiang Province Hangzhou 310014Zhejiang ProvinceChina

BACKGROUND Trauma is a common cause of pancreatic duct *** treatment is recommended in current clinical guidelines for adult pancreatic injury because non-surgical treatments have higher risks of serious complications or even death compared with surgical *** SUMMARY A 22-year-old woman was admitted to Tiantai People’s Hospital of Zhejiang Province after 1-h duration of abdominal pain and distension following *** diagnosis was“traumatic pancreatic rupture”.The patient’s symptoms were not severe,her vital signs were stable,and signs of peritonitis were not ***,conservative treatment could be considered,with the possibility of emergency surgery if *** 2 mo of conservative treatment with duct drainage,the pancreatic duct healed spontaneously with no significant *** We report a case of pancreatic duct disruption in the head and neck caused by trauma that was treated conservatively and healed spontaneously,providing a new choice for clinical *** isolated pancreatic injury with rupture of the pancreatic duct in the head and neck,conservative treatment under close observation is feasible.

关键词： Trauma Pancreatic ducts Conservative treatment Drainage Case report

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基于能量约束和历史信息的容迟网络路由算法

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北京理工大学学报 2009年第4期29卷 332-335页

作者：樊秀梅王明媚北京理工大学计算机科学技术学院智能信息技术北京市重点实验室北京100081

基于已有容迟网络(DTN)路由算法对能量考虑不足的问题,提出了基于能量约束和历史信息的DTN路由算法(ERHR),并在The One(the opportunistic networking environment)平台上,用Java语言进行仿真比较.仿真结果表明,ERHR在能量消耗、传递成... 详细信息

基于已有容迟网络(DTN)路由算法对能量考虑不足的问题,提出了基于能量约束和历史信息的DTN路由算法(ERHR),并在The One(the opportunistic networking environment)平台上,用Java语言进行仿真比较.仿真结果表明,ERHR在能量消耗、传递成功率和平均缓冲时间等性能上优于其他DTN路由算法,适应DTN网络的需求,是一种有效的DTN网络路由解决方案.

关键词：通信网络技术容迟网络(DTN) 路由协议

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Diversified calcimicrobes in dendrolites of the Zhangxia Formation, Miaolingian Series(Middle Cambrian) of the North China craton

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Journal of Palaeogeography 2021年第2期10卷 157-181页

作者： ming-Xiang Me Muhammad Riaz Zhen-Wu Zhang Qing-Fen Meng Yuan Hu School of Earth Sciences and Resources China University of GeosciencesBeijing 100083China State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesBeijing 100083China State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of TechnologyChengdu 610059China College of Energy Resources Chengdu University of TechnologyChengdu 610059China Centre for Geographical Information System University of the PunjabLahore 54590Pakistan.

As a type of non-laminated microbial carbonates, dendrolites are dominated by isolated dendritic clusters of calcimicrobes and are distinct from stromatolites and thrombolites. The dendrolites in the upper part of the Miaolingian Zhangxia Formation at Anjiazhuang section in Feicheng city of Shandong Province, China, provide an excellent example for further understanding of both growth pattern and forming mechanism of dendrolites. These dendrolites are featured by sedimentary fabrics and composition of calcified microbes as follows.(1) The strata of massive limestones, composed of dendrolites with thickness of more than one hundred meters, intergrade with thick-bedded to massive leiolites, formimg the upper part of a third-order depositional sequence that constitutes a forced regressive systems tract.(2) A centimeter-sized bush-like fabric(shrub) typically produced by calcified microbes is similar to the mesoclot in thrombolites but distinctive from clotted fabrics of thrombolites. This bushlike fabric is actually constituted by diversified calcified microbes like the modern shrub as a result of gliding mobility of filamentous cyanobacteria. Such forms traditionally include: the Epiphyton group(which actually has uncertain biological affinity), the Hedstroemia group which closely resembles modern rivulariacean cyanobacteria,and the possible calcified cyanobacteria of the Lithocodium–Bacinella group.(3) Significantly, dense micrite of leiolite is associated with sponge fossils and burrows, and is covered by microstromatolite. The Lithocodium–Bacinella group is a controversial group of interpreted calcified cyanobacteria in the Cambrian that has also been widely observed and described in the Mesozoic. Therefore, dendrolites with symbiosis of leiolites in the studied section provide an extraordinary example for further understanding of growing style of bush-like fabrics(shrubs) of the dendrolites dominated by cyanobacterial mats. Furthermore, the present research provid

关键词： Calcified microbe Dendrolite Leiolite Epiphyton Lithocodium–Bacinella Cambrian Miaolingian Zhangxia Formation

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Gain enhancement of perovskite nanosheets by a patterned waveguide:excitation and temperature dependence of gain saturation

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Light(Science & Applications) 2023年第12期12卷 2688-2699页

作者： Inhong Kim Ga Eul Choi ming mei Min Woo Kim Minju Kim Young Woo Kwon Tae-In Jeong Seungchul Kim Suck Won Hong Kwangseuk Kyhm Robert A.Taylor Department of Opto&Cogno Mechatronics Engineering RCDAMPPusan National UniversityBusan 46241Republic of Korea Department of Nano-Fusion Technology Pusan National UniversityBusan 46241Republic of Korea Clarendon Laboratory Department of PhysicsUniversity of OxfordOxford OX13PUUK

Optical gain enhancement of two-dimensional CsPbBr3 nanosheets was studied when the amplified spontaneous emission is guided by a patterned structure of *** the uncertainties and pitfalls in retrieving a gain coefficient from the variable stripe length method,a gain contour g((h))ω.x)was obtained in the plane of spectrum energy(hω)and stripe length(x),whereby an average gain was obtained,and gain saturation was *** and temperature dependence of the gain contour show that the waveguide enhances both gain and thermal stability due to the increased optical confinement and heat dissipation,and the gain origins were attributed to the two-dimensional excitons and the localized states.

关键词： waveguide saturation contour

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Pregnancy outcomes of the first thawing cycle in"freeze-all"strategy of infertility patients with fever during oocyte recruitment:a matched-pair study

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Chinese Medical Journal 2021年第7期134卷 800-805页

作者： ming-mei Lin Zi-Ru Niu Hua Zhang Rong Li Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China National Clinical Research Center for Obstetrics and Gynecology Beijing 100191China Beijing Key Laboratory of Assisted Reproductive Endocrinology and Assisted Reproductive Technology Beijing 100191China Research Center of Clinical Epidemiology Peking University third hospitalBeijing 100191China.

Background:It is currently unknown whether patients with a fever after controlled ovulation during egg retrieval could increase the risk of pelvic infection or not,and fever itself may affect endometrial receptivity or embryo quality with poor pregnancy *** aim of this study was to analyze the outcomes of patients with fever during oocyte retrieval after the first frozen-thawed embryo transfer(FET)***:This was a 1:3 retrospective paired study matched for *** this study,58 infertility patients(Group 1)had a fever during the control ovulation,and the time of the oocyte retrieval was within 72 hours,they underwent ovum pick up and whole embryo freezing("freeze-all"strategy).The control subjects(Group 2)are 174 patients matched for age who underwent whole embryo freezing for other *** baseline characteristics,clinical data of ovarian stimulation,and outcomes,such as the clinical pregnancy rate,ongoing clinical pregnancy rate were compared between the two groups in the subsequent FET ***:All patients had no pelvic inflammatory disease after oocyte ***-Mullerian hormone(AMH)levels(4.2 vs.2.2,P<0.001)were higher in group 2,and the number of oocytes retrieved,and fertilization rate were lower in group 1(P<0.001),but the endometrial thickness,the number of embryo transfers,and the type of luteal support supplementation were similar between the two *** pregnancy outcomes in the subsequent FET cycle,the implantation rate,clinical pregnancy rate,early spontaneous rate,ectopic pregnancy rate,and ongoing pregnancy rate were all not significantly *** regression analyses showed that the clinical pregnancy rate and ongoing pregnancy rate were also not significantly ***:Transvaginal ultrasound-guided follicular puncture for oocyte retrieval is a safe and minimally invasive method for patients with ***,the fever had almost no effect on embryo quality.

关键词： Fever Oocyte retrieval Infertility Pregnancy outcomes

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