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Antiapoptotic Effect of Gene Therapy with Recombinant Adenovirus Vector Containing Hypoxia-inducible Factor-1α after Cerebral Ischemia and Reperfusion in Rats

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Chinese Medical Journal 2017年第14期130卷 1700-1706页

作者： ming-lang Yang Tao Tao Jian Xu Zhi Liu Dan Xu Department of Rehabilitation Medicine Affiliated People&#39 s Hospital Guizhou Medical University Guiyang Guizhou 550002 China Department of Rehabilitation Medicine Guizhou Provincial People&#39 s Hospital Guiyang Guizhou 550002 China Department of Neurology Affiliated Hospital Guizhou Medical University Guiyang Guizhou 550001 China Department of Pharmacy Affiliated Hospital Guizhou Medical University Guiyang Guizhou 550001 China

Background：Mounting evidence has demonstrated that hypoxia-inducible factor-1α （HIF-1α） could attenuate brain injuries after cerebral ischemia and reperfusion （CIR）.However,few reports have addressed the therapeutic efficacies of a recombinant adenovirus vector containing HIF-1o （AdHIF-1o） gene after ischemia and *** aim of this study was to examine the antiapoptotic and neuroprotective effects ofAdHIF-1o gene for cerebral injuries after ischemia and reperfusion in ***：From February to December 2016,male Sprague-Dawley rats were randomly divided into normal,sham,CIR,AdHIF-1α,and recombinant adenovirus （Ad） *** cerebral artery occlusion model was established by Longa＇s method and reperfusion resumed at 2 h ***-1α solution,Ad solution,and phosphate-buffered saline were injected into the right lateral ventricle of rats in AdHIF-lα,Ad,and CIR *** tissue sections were observed under fluorescent microscope to confirm the definite expression of recombinant adenovirus in Ad and AdHIF-1o *** expressions of HIF-lα protein were analyzed by immunohistochemical staining at 6 h,24 h,and 72 h *** water content and neurological deficit scores were evaluated at 6 h,24 h,and 72 h *** brain injuries were examined after hematoxylin and eosin stain and nerve cell apoptosis was measured after terminal-deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL） stain at 72 h *** were conducted with one-way analysis of variance by post hoc Scheffe＇s test among different experimental ***：Green fluorescent protein was successfully expressed in brain tissue ofAd andAdHIF-1α groups from 24 h to 21 days *** detected by immunohistochemical staining,the expressions of HIF-lα protein were obviously enhanced in AdHIF-1o group than those in CIR and Ad groups at 24 h and 72 h postreperfusion,*** were significant reduct

关键词： Apoptosis Cerebral Ischemia Hypoxia-inducible Factor-1 α, Recombinant Adenovirus Vector Reperfusion

Role of different childbirth strategies on pelvic organ prolapse and stress urinary incontinence： a prospective study

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Chinese Medical Journal 2008年第3期121卷 213-215页

作者： ZHU Lan BIAN Xu-ming LONG Yan lang Jing-he Department of Obstetrics and Gynecology Peking Union MedicalCollege Hospital Beijing 100730 China

Background Traumatic damage to fascial and muscular support structures during childbirth may be a major factor of the development of stress urinary incontinence （SUI） and pelvic organ prolapse （POP）. The aim of this study was to prospectively investigate the role of different childbirth strategies on POP and SUI. Methods A total of 120 selected pregnant women were recruited and divided into two groups： vaginal delivery group （n=72） and selective cesarean delivery group （n=48）. The women were questioned with SUI sheet and estimated with POP quantification （POP-Q） within 6-8 weeks after delivery. The correlations of SUI and results of POP-Q with the strategies of delivery were analyzed, ttest, X2 test, and the Mann-Witney test were used for statistical analysis. Results Among the 120 women, SUI was developed during 29-30 gestational weeks in 20 patients （16.6%）, of whom 14 （70%） had symptoms of SUI till 6 weeks postpartum. Totally 20 （16.6%） women had SUI symptoms after delivery. The prevalence of postpartum SUI was positively correlated with the occurrence of SUI during 29-30 gestational weeks （P〈0.001）. In the vaginal delivery group, 100% of the women suffered form urinary prolapse 6 weeks postpartum, while 87.5% of those in the selective cesarean delivery group developed POP （P〈0.01）. The percentages of the first- and second-degree urinary prolapse in the vaginal delivery group were 20.8% and 79.2% respectively, which were significantly different from those in the selective cesarean delivery group （64.6% and 22.9%; both P=0.000）. Conclusions Pregnancy and delivery play an etiologic role in the development of SUI and POP. The onset of SUI during late pregnancy implies a significant risk of postpartum SUI symptoms. The prevalence of POP is significantly higher after vaginal delivery than after cesarean delivery.

关键词： natural childbirth cesarean section urinary incontinence pelvic organ prolapse

Micro-structured lepidocrocite-type H_(1.07)Ti_(1.73)O_(4)as anode for lithium-ion batteries with an ultrahigh rate and long-term cycling performance

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Rare Metals 2021年第6期40卷 1391-1401页

作者： Li-Juan Hou Rui-Chao Liu Hui-Yu Yuan De-Zhi Kong Wei-Xia Shen Jin-Hao Zang Juan Guo Shu-Ge Dai ming-lang Wang Ting-Ting Xu Xin-Jian Li Ye Wang Key Laboratory of Material Physics of Ministry of Education School of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450052China School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450052China Key Laboratory of Henan Province for High Temperature Functional Materials Zhengzhou 450000China Shandong Key Laboratory of Medical Physics and Image Processing&Shandong Provincial Engineering and Technical Center of Light Manipulations School of Physics and ElectronicsShandong Normal UniversityJinan 250358China

The lepidocrocite-type H_(1.07)Ti_(1.73)O_(4) microsized structures with a tap density of 0.88 g·cm^(-3) were prepared through the ion exchange method with K_(0.8)Li_(0.27)Ti_(1.73)O_(4) powder as the precursor,and they exhibited good rate performance and outstanding cycle stability as an anode material for lithium ion batteries(LIB).The ion exchange method provides favorable conditions for H_(1.07)Ti_(1.73)O_(4) as an anode electrode material for LIBs.X-ray photoelectron spectroscopy(XPS)result demonstrates the existence of defects in the nonstoichiometric H1.07Ti1.73O4,which have a beneficial effect on the LIB *** electrochemical performance test proves that the half-cell with microsized H_(1.07)Ti_(1.73)O_(4)as the anode electrode can maintain a specific capacity of 129.5 mAh·g^(-1) after 1100 cycles and 101 mAh·g^(-1)after 3000 long cycles at high current densities of 2.0 and 5.0 A·g^(-1),*** addition,the small volume change rate of 3.6%in H_(1.07)Ti_(1.73)O_(4)during Li ion insertion was confirmed by real-time in situ transmission electron microscopy(TEM).The LiFePO_(4)||H_(1.07)Ti_(1.73)O_(4)full battery exhibits a longterm cycling stability with a specific capacity of73.8 mAh·g^(-1) at a current density of 500 mA·g^(-1) after 200 cycles.

关键词： Lepidocrocite-type H_(1.07)Ti_(1.73)O_(4) Anode material of lithium ion batteries Long cycle stability

Theoretical design of single-molecule NOR and XNOR logic gates by using transition metal dibenzotetraaza[14]annulenes

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Chinese Physics B 2020年第6期29卷 411-418页

作者： Zi-Qun Wang Fei Tang Mi-Mi Dong ming-lang Wang Gui-Chao Hu Jian-Cai Leng Chuan-Kui Wang Guang-Ping Zhang Shandong Key Laboratory of Medical Physics and Image Processing&Shandong Provincial Engineering and Technical Center of Light Manipulations School of Physics and ElectronicsShandong Normal UniversityJinan 250358China Department of Physics School of Electronic and Information EngineeringQilu University of Technology (Shandong Academy of Sciences)Jinan 250353China

The idea of replacing traditional silicon-based electronic components with the ones assembled by organic molecules to further scale down the electric circuits has been attracting extensive research *** the molecularly assembled components,the design of molecular logic gates with simple structure and high Boolean computing speed remains a great ***,by using the state-of-the-art nonequilibrium Green’s function theory in conjugation with first-principles method,the spin transport properties of single-molecule junctions comprised of two serially connected transition metal dibenzotetraaza[14]annulenes(TM(DBTAA),TM=Fe,Co)sandwiched between two single-walled carbon nanotube electrodes are theoretically *** numerical results show a close dependence of the spin-resolved current-voltage characteristics on spin configurations between the left and right molecular kernels and the kind of TM atom in TM(DBTAA)*** taking advantage of spin degree of freedom of electrons,NOR or XNOR Boolean logic gates can be realized in Fe(DBTAA)and Co(DBTAA)junctions depending on the definitions of input and output *** work proposes a new kind of molecular logic gates and hence is helpful for further miniaturization of the electric circuits.

关键词： single-molecule junction molecular logic gate spin transport nonequilibrium Green’s function method

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M310核电站非能动安全改进措施研究

原子能科学技术 2015年第7期49卷 1184-1190页

作者：邹象郎明刚清华大学核能与新能源技术研究院北京100084

本文利用MELCOR1.8.5程序建立了典型的M310核电站的严重事故模型,基于该模型设计了多种非能动的缓解措施,针对由全厂断电诱发的严重事故,模拟研究了这些非能动安全措施的缓解效果。研究结果表明:在全厂断电事故下,堆芯补水箱系统、堆腔... 详细信息

本文利用MELCOR1.8.5程序建立了典型的M310核电站的严重事故模型,基于该模型设计了多种非能动的缓解措施,针对由全厂断电诱发的严重事故,模拟研究了这些非能动安全措施的缓解效果。研究结果表明:在全厂断电事故下,堆芯补水箱系统、堆腔注水系统、非能动余热排出系统均能有效地投入使用,并显著地延缓事故的发展,将核电站稳定在一个安全的状态,为人工干预赢得更多时间。

关键词： M310核电站非能动系统全厂断电 MELCOR1.8.5程序

Low-Bias Conductance Mechanism of Diarylethene Isomers:a First-Principle Study

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Chinese Journal of Chemical Physics 2020年第6期33卷 697-702,I0002页

作者： ming-lang Wang Guang-ping Zhang Xiao-xiao Fu Chuan-kui Wang School of Physics and Electronics Shandong Normal UniversityJinan 250358China

The structure-property relationship of diarylethene(DAE)-derivative molecular isomers,which involve ring-closed and ring-open forms,is investigated by employing the nonequilibrium Green’s function formalism combined with density functional *** junctions are formed by the isomers connecting to Au(111)electrodes through flanked pyridine *** difference in electronic structures caused by different geometry structures for the two isomers,particularly the interatomic alternative single bond and double bond of the ring-closed molecule,contributes to the vastly different low-bias conductance *** lowest unoccupied molecular orbital(LUMO)of the isomers is the main channel for electron *** addition,more electrons transferred to the ring-closed molecular junction in the equilibrium condition,thereby decreasing the LUMO energy to near the Fermi energy,which may contribute to a larger conductance value at the Fermi *** findings are helpful for understanding the mechanism of low-bias conductance and are conducive to the design of high-performance molecular switching based on diarylethene or diarylethene-derivative molecules.

关键词： Molecular electronics Molecular switching Density functional theory Nonequilibrium Green’s function

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细胞周期分析新方法

分析化学 2019年第9期47卷 1293-1301页

作者：方涵书郎明非孙晶大连大学医学院大连大学环境与化学工程学院

细胞周期是细胞生命活动的基本过程,对细胞周期的分析能更深入地了解疾病发生的本质。目前,分析细胞周期的方法以流式细胞术为主,主要是通过检测细胞内DNA含量和细胞周期特异性蛋白而分析细胞周期。随着分析技术的发展,细胞周期分析在... 详细信息

细胞周期是细胞生命活动的基本过程,对细胞周期的分析能更深入地了解疾病发生的本质。目前,分析细胞周期的方法以流式细胞术为主,主要是通过检测细胞内DNA含量和细胞周期特异性蛋白而分析细胞周期。随着分析技术的发展,细胞周期分析在图像分析、单细胞分析、减少样本量、提高通量等方面不断地提高和改进,近年出现了许多分析细胞周期的新方法,如成像流式细胞术、质谱流式细胞术、微流控技术、拉曼光谱法等,这些方法极大地丰富了细胞周期的检测方法,提高了细胞周期测量的精确度。本文对细胞周期的新分析方法进行了介绍,并对其发展方向进行了展望。

关键词：细胞周期细胞周期蛋白 DNA含量微流控技术流式细胞术评述

Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper,Sogatella furcifera

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Insect Science 2024年第4期31卷 1015-1025页

作者： Meng-Qi Zhang lang-lang Gong Ya-Qin Zhao Yun-Feng Ma Gui-Jun Long Huan Guo Xuan-Zheng Liu J.Joe Hull Youssef Dewer Chao Yang Ning-Ning Zhang ming He Peng He National Key Laboratory of Green Pesticides Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyangChina Pest Management and Biocontrol Research Unit US Arid Land Agricultural Research CenterUSDA Agricultural Research ServicesMaricopaArizonaUSA Phytotoxicity Research Department Central Agricultural Pesticide LaboratoryAgricultural Research CenterDokkiGizaEgypt Guizhou Jifeng Seed Industry Limited Liability Company XingyiGuizhou ProvinceChina Shandong Facility Horticulture Bioengineering Research Center Weifang University of Science and TechnologyWeifangShandong ProvinceChina

Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shells,and/or are difficult to collect and maintain outside of their normal *** is the case with Sogatella furcifera,the white-backed planthopper(WBPH),a significant pest of Oryza sativa(rice)that oviposits inside rice *** extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice *** bypass these issues,we targeted embryos prior to oviposition via direct parental(DIPA)-CRISPR,in which Cas9 and single-guide RNAs(sgRNAs)for the WBPH eye pigment gene tryptophan 2,3-dioxygenase were injected into the hemocoel of adult *** at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene-editing *** evaluation of the offspring indicated that the highest G0 gene-edited efficacy(56.7%)occurred in females injected 2 d posteclosion,and that those mutations were heritably transmitted to the G1 *** study demonstrates the potential utility of DIPA-CRISPR for future gene-editing studies in non-model insect species and can facilitate the development of novel pest management applications.

关键词： CRISPR/Cas9 DIPA-CRISPR gene editing tryptophan 2,3-dioxygenase WBPH

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语言价值观与语言多样性

云南师范大学学报（哲学社会科学版） 2019年第5期51卷 57-64页

作者：周明朗马里兰大学语言学院

文章聚焦语言价值观与语言多样性的关系,认为语言价值观呈现语言意识形态,涵盖物质价值观和精神价值观。前者生成于语言市场,凭借强势语言追逐物质利益。后者根植于对精神家园的信念,依靠母语守望精神家园。依据对中国语言文字使用调查... 详细信息

文章聚焦语言价值观与语言多样性的关系,认为语言价值观呈现语言意识形态,涵盖物质价值观和精神价值观。前者生成于语言市场,凭借强势语言追逐物质利益。后者根植于对精神家园的信念,依靠母语守望精神家园。依据对中国语言文字使用调查资料的分析,研究显示,物质价值观与语言的客观活力有关联,但是并不一定可以维护弱势语言的活力,因为强势语言总是享有更多资源;语言精神价值观与语言的主观活力相关,可以保护弱势语言的活力,因为健康的精神价值观可以助力个人和社区保持双语平衡。研究说明,保护语言多样性是国家、社区和个人的共同使命。只有这三者都端正语言价值观,三方合作,才可落实《岳麓宣言》达成的国际共识。

关键词：语言价值观语言多样性物质价值观精神价值观客观活力主观活力