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Identification and characterization of cell cultures with various embryogenic/regenerative potential in cotton based on morphological,cytochemical,and cytogenetical assessment

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Journal of Integrative Agriculture 2019年第1期18卷 1-8页

作者： GUO Hui-hui WU jian-fei CHEN Cui-xia WANG Hong-mei zhao Yun-lei ZHANG Chao-jun JIA Yin-hua LIU Fang NING Tang-yuan CHU zhao-hui ZENG Fan-chang State Key Laboratory of Crop Biology Ministry of Science and Technology/College of AgronomyShandong Agricultural University State Key Laboratory of Cotton Biology Ministry of Science and Technology/Cotton Research InstituteChinese Academy of Agricultural Sciences Key Laboratory of Water Resources and Drought Resistant Germplasm Improvement Ministry of Agriculture

Somatic embryogenesis(SE) plays a vital role in genetic transformation and massive propagation of important agronomical and economical ***,we conducted a systematic assessment of the morphological,cytochemical,and cytogenetical characteristics of six culture strains with various embryogenic/regenerative potential during SE process in *** indicated that the six cell culture strains had stable ploidy levels,and did not reveal any relationship between the cytogenetic state and their morphogenetic ***,the six culture strains were compared via double staining with Evans blue and Acetocarmine to efficiently distinguish embryogenic and non-embryogenic cells and determine the embryogenic nature of the *** addition,the kind of auxins added in medium affected not only growth property,color,size of cell clumps but also ploidy level and regeneration *** combining analysis of morphological,cytochemical,and cytogenetical characteristics of the cell cultures,we are able to obtain and maintain homogeneous cell population with high morphogenic and regeneration ability and establish efficient somatic embryogenesis and regeneration system from short-term cell cultures in upland cotton,which highlight the application of biotechnological approaches in crop breeding,and above all,to better understand totipotency of cells in higher plants.

关键词： cotton somatic embryogenesis cotton cell culture strains cytochemical EB-AC double staining cytogenetical ploidy stability embryogenic/regenerative potential, morphology

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Thermoacoustic assessment of hematocrit changes in human forearms

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Chinese Physics B 2021年第9期30卷 284-289页

作者： Xue Wang Rui zhao Yi-Tong Peng Zi-Hui Chi Zhu Zheng En Li Lin Huang Hua-Bei jian School of Electronic Science and Engineering(National Exemplary School of Microelectronics) University of Electronic Science and Technology of ChinaChengdu 611731China Center for Information in Medicine University of Electronic and Technology of ChinaChengdu 611731China Department of Medical Engineering University of South FloridaTampa FL 33620USA School of Optoelectronic Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065China

Abnormal hematocrit(Hct)is associated with an increased risk of pre-hypertension and all-cause death in general population,and people with a high Hct value are susceptible to arterial cardiovascular disease and venous *** this study,we report for the first time on the ability of thermoacoustic imaging(TAI)for in vivo evaluating Hct changes in human *** vitro blood samples with different Hct values from healthy volunteers(n=3)were prepared after *** was performed using these samples in comparison with the direct measurements of *** vivo TAI was conducted in the forearm of healthy volunteers(n=7)where Hct changes were produced through a vascular occlusion stimulation over a period of *** results of in vitro blood samples obtained from the 3 healthy subjects show that the thermoacoustic(TA)signals changes due to the variation of blood conductivity are closely related to the changes in *** addition,the in vivo TA signals obtained from the 7 healthy subjects consistently increase in the artery/muscle and decrease in the vein during venous or arterial occlusion because of the changed Hct value in their *** findings suggest that TAI has the potential to become a new tool for monitoring Hct changes for a variety of pre-clinical and clinical applications.

关键词： thermoacoustic imaging hematocrit change human forearm

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Feasibility Study on Chemical Flooding in Super High Porosity and Permeability Heavy Oil Reservoir

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Journal of Materials Science and Chemical Engineering 2020年第11期8卷 43-53页

作者： Juan zhao jian Zhang Guang Yang China State Key Lab of Offshore Oil Exploitation Beijing China CNOOC Research Institute CO. Ltd. Beijing China

In this paper, the feasibility study of chemical flooding is carried out for ultra-high porosity and high permeability heavy oil field with permeability higher than 10 μm2 and porosity greater than 35%. The viscosity-concentration relationship of four kinds of oil flooding systems such as hydrolyzed polyacrylamide, structural polymer A, structural polymer B and gel was studied. The results showed that the viscosity of ordinary polymer and structural polymer B was lower compared with other two types of oil displacement agents, and the viscosity of structural polymer A was higher. The higher the concentration, the higher the viscosity retention rate. The gel system has the highest viscosity and best anti-shear ability. The resistance coefficient and residual resistance coefficient of structural polymer A and gel system were further studied. The results show that permeability, velocity and polymer concentration all affect the resistance coefficient and residual resistance coefficient. From the point of view of resistance establishment ability, it is considered that structural polymer A is not suitable for permeability formation above 10 μm2. Gel system has stronger ability to establish resistance coefficient than structural polymer A flooding system, and it is more feasible for formation system with permeability above 10 μm2.

关键词： Super High Porosity and High Permeability Chemical Flooding Viscosity-Concentration Relationship Resistance Coefficient Coefficient of Residual Resistance

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鄂尔多斯盆地南部中生界大中型油田形成条件与勘探策略

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石油勘探与开发 2006年第2期33卷 145-149页

作者：王建民西安石油大学资源工程系

为查明鄂尔多斯盆地南部中生界油气分布规律,寻求现实有效的勘探部署策略,以尽快实现选区油气突破和油田(藏)发现,通过对其石油地质条件的系统分析和勘探开发实践的思考总结,认为鄂南中生界油气既具有大面积生成、分布、富集并连片成藏... 详细信息

为查明鄂尔多斯盆地南部中生界油气分布规律,寻求现实有效的勘探部署策略,以尽快实现选区油气突破和油田(藏)发现,通过对其石油地质条件的系统分析和勘探开发实践的思考总结,认为鄂南中生界油气既具有大面积生成、分布、富集并连片成藏的特点,又在不同层位和空间上聚散不一,呈现出复杂的变化格局;油藏类型以隐蔽性的构造-岩性复合油藏及岩性油藏为主,大型有利沉积相带与低幅度局部构造及其集中分布、组合发育的大规模低幅度构造背景是控制油气大面积聚集成藏的主要因素,也是大中型油田发育的基本条件。结论:在鄂南中生界这样一个具备大中型油田发育条件但勘探手段颇受限制的地区,应该兼顾上下油层,以钻井为主要手段,实行规模化勘探策略。

关键词：鄂尔多斯盆地南部中生界油气分布隐蔽性油藏大中型油田规模化勘探策略

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The World's Salt Lakes:Today and Tomorrow

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Acta Geologica Sinica(English Edition) 2014年第S1期88卷 17-18页

作者： ZHENG Mianping HAO Ziguo FEI Hongcai HUANG min ZHOU jian Susan TURNER Chairman of the Scientific Program Committee of 12th International Conference The Institute of Mineral ResourcesChinese Academy of Geological Sciences Chinese Academy of Geological Sciences Editorial Office of Acta Geologica Sinica (English edition) Geological Society of China

The 12th International Conference on Salt Lake Research,which is organized jointly by International Society of Salt Lake and The Institute of mineral Resources,Chinese Academy of Geological Sciences,will be held on July 14–18,2014 in Langfang,Hebei Province,*** conference themes focus on future and global salt lake sustainable research and development and will bring together international specialists in limnology,sedimentology,geology,geochemistry,biology,

关键词： Lake The World’s Salt Lakes

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A NEW TRITERPENOID FROM SANGUISORBA ALPINA

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Chinese Chemical Letters 1992年第7期3卷 515-516页

作者： Xiang Qian LIU Zi min LIU Zhong jian JIA Xiu Wen HAN Institute of Organic Chemistry State Key Laboratory of Applied Organic ChemistryLanzhou UniversityLanzhou 730000

From the roots of Sanguisorba alpina, a new triterpenoid was isolated and identified as 2-isobutyryloxy-19α, 25-dihydroxyursa-1, 12-dien-28-oic acid by means of spectral methods, named as aipinic acid.

关键词： ppm A NEW TRITERPENOID FROM SANGUISORBA ALPINA CCH show

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MRI shows clodronate-liposomes attenuating liver injury in rats with severe acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2010年第2期9卷 192-200页

作者： Zhang, jian-Xin Dang, Sheng-Chun Zhang, Yong Sha, Xin Zhang, Li-Rong Wei, Chuan-She Chen, min jiang, De-Li jiangsu Univ Affiliated Hosp Dept Gen Surg Zhenjiang 212001 Peoples R China jiangsu Univ Affiliated Hosp Dept MRI Zhenjiang 212001 Peoples R China jiangsu Univ Sch Chem & Chem Engn Zhenjiang 212013 Peoples R China

BACKGROUND: Studies have revealed that macrophages play an important role in the development of severe acute pancreatitis (SAP). Activated macrophages can lead to a systemic inflammatory response, induce lipid peroxidation, impair membrane structure, result in injury to the liver and the other extrahepatic organs, and eventually result in multiple organ dysfunction syndrome by promoting excessive secretion of cytokines. Liver injury can further aggravate the systemic inflammatory response and increase mortality by affecting the metabolism of toxins and the release of excessive inflammatory mediators. Clodronate is a synthetic bisphosphonate, which is often used for treating bone changes caused by osteoporosis and other factors. In the current study, we created liposomes containing superparamagnetic iron oxide particles (SPIOs) for macrophage labeling and magnetic resonance imaging, using a novel method that can bind the clodronate to induce apoptosis and deplete macrophages. METHODS: Superparamagnetic Fe(3)O(4) nanoparticles were prepared by chemical coprecipitation. SPIO-containing liposomes and SPIO-clodronate-containing liposomes were prepared by the thin film method. SAP models were prepared by injection of sodium taurocholate (2 ml/kg body weight) into the subcapsular space of the pancreas. Sprague-Dawley rats were randomly divided into a control group, a SAP plus SPIO-liposome group, and a SAP plus SPIO-clodronate-containing group. Two and six hours after SAP models were available, T2-weighted MRI scans (in the same plane) of the livers of rats in each group were performed. At the end of the scans, 2 ml of blood was taken from the superior mesenteric vein to measure the levels of serum amylase, ALT, AST, TNF-alpha, and IL-6. Pathological changes in the liver and pancreas were assessed. RESULTS: Transmission electron microscopy showed that the liposomes had a uniform size. No pathological changes in the pancreata of rats in the control group were noted. The pat

关键词： pancreatitis, acute clodronate disodium liver injury MR imaging macrophage

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Crystallization and Preliminary Crystallographic Studies of Luffaculin 1,a Ribosome-inactivating Protein from the Seeds of Luffa Acutangula

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Chinese Journal of Structural Chemistry 2006年第9期25卷 1035-1038页

作者： HOU Xiao-min CHEN ming-Huang XIE Jie-ming YE Xiao-ming zhao Geng-Xiang YANG Feng CHEN Li-Qing HUANG ming-Dong state Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter The Chinese Academy of Sciences Fuzhou Fujian 350002 China Graduate School of the Chinese Academy of Sciences Beijing 10039 China Fujian Medical University Fuzhou 350004 China Laboratory. for Structural Biology Department of Chemistry Graduate Programs of BiotechnologyChemistry and Materials Science University of Alabama in Huntsville Huntsville AL 35899 USA

Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813, c = 83.571 A, α = 891068,β = 80.009 and y = 72.143°, and has two molecules per asymmetric unit. X-ray data have been collected to be 1.4 A using a synchrotron source.

关键词： Luffa acutangula luffaculin 1 ribosome-inactivating proteins crystallization

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Fabrication of Tl_(2)Ba_(2)CaCu_(2)O_(8) superconducting films without thallium pellets

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Chinese Physics B 2018年第5期27卷 464-468页

作者： Teng-Da Xu jian Xing Li-Tian Wang Jin-Li Zhang Sheng-Hui zhao Yang Xiong Xin-Jie zhao Lu Ji Xu Zhang ming He College of Electronic Information and Optical Engineering Nankai UniversityTianjin 300350China Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin Tianjin 300350China

A new improved two-step method in fabricating Tl_(2)Ba_(2)CaCu_(2)O_(8)(Tl-2212)thin films is presented in this *** the first process of dc magnetron sputtering,the thallium content in the precursor film is largely increased by adjusting the ratio of thallium in the sputtering *** the second annealing process in the absence of additional thallium pellets or powder source,high-quality Tl-2212 thin films can be *** proper content of thallium in the precursor film provides a relatively stable atmosphere to guarantee the growth of Tl-2212 *** method avoids the repeated production of the thallium pellets in the post-annealing process,the repeatability and controllability of the experiment are greatly improved.X-ray diffraction(XRD)scans show that all of the sharp peaks of the sample films can be assigned to the(00 l)peaks of Tl-2212 *** highest superconducting critical temperature(T_(c))of the films is 105 K and the critical current density(J_(c))can achieve 1.93 MA/cm^(2) in zero magnetic field at 77 K for a 600 nm film.

关键词： Tl-2212 superconducting films post-annealing critical temperature critical current density

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Warm ischemia time and elevated serum uric acid are associated with metabolic syndrome after liver transplantation with donation after cardiac death

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World Journal of Gastroenterology 2018年第43期24卷 4920-4927页

作者： Liang-Shuo Hu Yi-Chao Chai Jie Zheng jian-Hua Shi Chun Zhang min Tian Yi Lv Bo Wang Ai Jia Department of Hepatobiliary Surgery First Affiliated Hospital of Xi’an Jiaotong University Clinical Research Center First Affiliated Hospital of Xi’an Jiaotong University Department of Gastroenterology First Affiliated Hospital of Xi’an Jiaotong University

AIM To describe the prevalence of posttransplant metabolic syndrome(PTMS) after donation after cardiac death(DCD) liver transplantation(LT) and the pre-and postoperative risk *** One hundred and forty-seven subjects who underwent DCD LT from January 2012 to February 2016 were enrolled in this study. The demographics and the clinical characteristics of pre-and post-transplantation were collected for both recipients and donors. PTMS was defined according to the 2004 Adult Treatment Panel-Ⅲ criteria. All subjects were followed monthly for the initial 6 mo after discharge, and then, every 3 mo for 2 years. The subjects were followed every 6 mo or as required after 2 years *** The prevalence of PTMS after DCD donor orthotopic LT was 20/147(13.6%). Recipient's body mass index(P = 0.024), warm ischemia time(WIT)(P = 0.045), and posttransplant hyperuricemia(P = 0.001) were significantly associated with PTMS. The change in serum uric acid levels in PTMS patients was significantly higher than that in non-PTMS patients(P < 0.001). After the 1 s t mo, the level of serum uric acid of PTMS patients rose continually over a period, while it was unaltered in non-PTMS patients. After transplantation, the level of serum uric acid in PTMS patients was not associated with renal *** PTMS could occur at early stage after DCD LT with growing morbidity with the passage of time. WIT and post-LT hyperuricemia are associated with the prevalence of PTMS. An increased serum uric acid level is highly associated with PTMS and could act as a serum marker in this disease.

关键词： Posttransplant metabolic syndrome Liver transplantation Donation after cardiac death Uric acid Warm ischemia time

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