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Differential neuronal reprogramming induced by NeuroD1 from astrocytes in grey matter versus white matter

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Neural Regeneration Research 2020年第2期15卷 342-351页

作者： min-Hui Liu Wen Li jia-Jun Zheng yu-Ge Xu Qing He Gong Chen Guangdong-HongKong-Macao Institute of CNS Regeneration (GHMICR) Jinan UniversityGuangzhouGuangdong ProvinceChina Department of Biology The Huck Institutes of Life SciencesThe Pennsylvania State UniversityUniversity ParkPAUSA

A new technology called in vivo glia-to-neuron conversion has emerged in recent years as a promising next generation therapy for neural regeneration and repair. This is achieved through reprogramming endogenous glial cells into neurons in the central nervous system through ectopically expressing neural transcriptional factors in glial cells. Previous studies have been focusing on glial cells in the grey matter such as the cortex and striatum, but whether glial cells in the white matter can be reprogrammed or not is unknown. To address this fundamental question, we express NeuroD1 in the astrocytes of both grey matter(cortex and striatum) and white matter(corpus callosum) to investigate the conversion efficiency, neuronal subtypes, and electrophysiological features of the converted neurons. We discover that NeuroD1 can efficiently reprogram the astrocytes in the grey matter into functional neurons, but the astrocytes in the white matter are much resistant to neuronal reprogramming. The converted neurons from cortical and striatal astrocytes are composed of both glutamatergic and GABAergic neurons, capable of firing action potentials and having spontaneous synaptic activities. In contrast, the few astrocyte-converted neurons in the white matter are rather immature with rare synaptic events. These results provide novel insights into the differential reprogramming capability between the astrocytes in the grey matter versus the white matter, and highlight the impact of regional astrocytes as well as microenvironment on the outcome of glia-toneuron conversion. Since human brain has large volume of white matter, this study will provide important guidance for future development of in vivo glia-to-neuron conversion technology into potential clinical therapies. Experimental protocols in this study were approved by the Laboratory Animal Ethics Committee of Jinan University(approval No. IACUC-20180321-03) on March 21, 2018.

关键词： astrocyte conversion efficiency corpus callosum cortex grey matter in vivo cell conversion NeuroD1 neuron reprogramming striatum white matter

Myotonic dystrophy type 1 presenting with dyspnea:A case report

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World Journal of Clinical Cases 2022年第20期10卷 7060-7067页

作者： yu-Xi jia Chun-Ling Dong jia-Wei Xue Xiao-Qin Duan ming-yu Xu Xiao-min Su Ping Li Department of Orthopedics The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina Application Demonstration Center of Precision Medicine Molecular Diagnosis The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina Department of Respiratory and Critical Care Medicine The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina Department of Rehabilitation Medicine The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina Department of Developmental Pediatrics The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina

BACKGROUND Myotonic dystrophy type 1(DM1)is a genetic neuromuscular disease involving multiple systems,especially the cardiopulmonary *** clinical phenotype of DM1 patients is highly variable,which limits early diagnosis and *** the present study,we reported a 35-year-old female DM1 patient with dyspnea as the primary onset clinical manifestation,analyzed her family's medical history,and reviewed related *** SUMMARY A 35-year-old woman was admitted to the hospital with dyspnea of 1 mo duration,and sleep apnea for 3 *** respiratory pattern and effort were normal,but limb muscle tension was *** into the patient's medical history revealed that she might have hereditary neuromuscular *** showed that her myotonia potentials were visible in the resting state of the examined muscles,with decreased motor unit potential time limit and *** testing for DM1 revealed that the cytosine-thymine-guanine(CTG)repeat number of the DMPK gene exceeded 50,while cytosine-CTG expansion in intron 1 of ZNF9 gene was<30 *** patient was diagnosed with *** DM1 is a genetic neuromuscular disease involving multiple systems,and the clinical phenotype in DM1 is extremely *** patients with DM1 may be presented at the respiratory department because of dyspnea,which should be cautioned by the *** may be no obvious or specific symptoms in the early stage of disease,and clinicians should improve their understanding of DM1 and make an early diagnosis,which will improve patients’quality of life.

关键词： Myotonic dystrophy type 1 DMPK gene Respiratory insufficiency Sleep apnea Non-invasive ventilation

Vacuum joints of dispersion-strengthened copper and its applications in synchrotron radiation front end

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Nuclear Science and Techniques 2016年第6期27卷 74-78页

作者： Yong-Jun Li Song Xue min Zhang Dan-Dan jia Dong-Bai Dou yu Gao Shanghai Institute of Applied Physics Chinese Academy ofSciencesZhangJiangShanghai 201204China University of Chinese Academy of Sciences Beijing 100049China SKY Technology Development Co. LtdChinese Academyof SciencesShenyang 110179China

Masks are critical elements of synchrotron radiation front end that are exposed to high temperature and *** absorber material is typically comprised of dispersion-strengthened copper,which can retain high performance at elevated *** processes under vacuum,including brazing and electron beam welding,are novel approaches for prolonging the absorber and for reducing power *** mechanical properties of brazed joints and electron beam welded joints of dispersion-strengthened copper workpieces are evaluated by tensile testing at 20,100,and 200 *** testing results indicate that the tensile strength and elongation of both vacuum joints decrease with increasing *** to brazed joints,electron beam welded joints have higher tensile strength,better ductility,and more stable performance.A novel welded mask with a total length of 600 mm is presented and shown to be practical for use in the highest heat load front end in the Shanghai synchrotron radiation facility phase-Ⅱ beamline project.

关键词：上海同步辐射装置弥散强化铜焊接接头前端真空电子束焊接应用钎焊接头

典型人工景观池塘水体甲烷释放特征及其对富营养化的响应

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湖泊科学 2025年第1期37卷 122-131,I0003-I0010页

作者：赖世平刘佳肖尚斌陈敏张博文孟江槐李元正三峡大学水利与环境学院宜昌443002 三峡库区生态环境教育部工程研究中心宜昌443002 三峡水库生态系统湖北省野外科学观测研究站宜昌443002 三峡大学水利工程博士后科研流动站宜昌443002

富营养化水体的碳通量过程是全球水域生态系统中的前沿和热点问题。本研究以三峡大学校园内景观池塘(莲心湖)为研究对象,开展为期一年的水气界面CH_(4)通量监测,并在此基础上进一步探讨水体富营养化水平对CH_(4)释放的影响。结果表明,... 详细信息

富营养化水体的碳通量过程是全球水域生态系统中的前沿和热点问题。本研究以三峡大学校园内景观池塘(莲心湖)为研究对象,开展为期一年的水气界面CH_(4)通量监测,并在此基础上进一步探讨水体富营养化水平对CH_(4)释放的影响。结果表明,莲心湖水气界面CH_(4)平均排放通量为5.06 mg/(m^(2)·h),整体表现为CH_(4)的源,且以冒泡释放为主导(占比为88.56%)。CH_(4)排放通量呈现时间异质性,表现为夏季(8.70 mg/(m^(2)·h))>冬季(4.80 mg/(m^(2)·h))>春季(3.88 mg/(m^(2)·h))>秋季(2.87 mg/(m^(2)·h)),白天(0.11 mg/(m^(2)·h))略高于夜晚(0.09 mg/(m^(2)·h))。CH_(4)排放通量在各个季节及昼夜的差异与温度、风速和叶绿素a等环境因素变化有关。随着水体富营养化水平的提高,CH_(4)排放通量呈现非线性增加的趋势,重度富营养化水体的CH_(4)排放通量是中度富营养化水体的2倍,这表明降低水体富营养化水平可有效减少水体的CH_(4)排放。研究结果可为其他同类型水体的温室气体减排提供参考。

关键词：富营养化甲烷释放池塘冒泡通量叶绿素a

The effects of the unsaturated degree of long-chain fatty acids on the rumen microbial protein content and the activities of transaminases and dehydrogenase in vitro

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Journal of Integrative Agriculture 2016年第2期15卷 424-431页

作者： GAO jian JING yu-jia WANG Meng-zhi SHI Liang-feng LIU Shi-min College of Animal Science and Technology Yangzhou University Institute of Agriculture The University of Western Australia

This study investigated the effects of the degree of unsaturation（unsaturity） of long-chain fatty acids on microbial protein content and the activities of transaminases and dehydrogenase in vitro using goat rumen fluid as the cultural *** types of fatty acids,stearic acid（C18：0,group A,control group）,oleic acid（C18：1,n-9,group B）,linoleic acid（C18：2,n-6,group C）,α-linolenic acid（C18：3,n-3,group D）,arachidonic acid（C20：4,n-6,group E）,and eicosapentaenoic acid（C20：5,n-3,group F）,were tested,and the inclusion ratio of each fatty acid was 3%（w/w） in total of culture *** were taken at 0,3,6,9,12,18 and 24 h,respectively,during culture for *** with stearic acid,linoleic acid,a-linolenic acid,and arachidonic acid increased the bacterial protein content,while oleic acid and eicosapentaenoic acid had no significant effects;the protozoal protein content was reduced for all the unsaturated fatty acids,and the magnitude of the reduction appeared to be associated with the degree of unsaturity of fatty *** total microbial protein content was dominantly accounted by the protozoal protein content（about 4-9 folds of the bacterial protein）,and only increased by linoleic acid,but reduced by oleic acid,arachidonic acid and eicosapentaenoic *** were no significant effects in the activities of both glutamic oxaloacetic transaminase（GOT） and glutamic-pyruvic transaminase（GPT） for all the other fatty acids,except for arachidonic acid which enhanced GOT activity and oleic acid which enhanced GPT *** total dehydrogenase activity was positively correlated with the degree of unsaturation of fatty *** conclusion,the inclusion of 3%of long-chain unsaturated fatty acids increased bacterial protein content,whereas reduced protozoal protein content and enhanced dehydrogenase *** fatty acids with more than three double bonds had detrimental effects on the microbial protein *** work demonstrates for the first time the eff

关键词： long-chain fatty acid rumen microbes goats protein concentration

Syntheses of three terbium complexes as fluorescent probes and their application on the pH detection of routine urine test

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Chinese Chemical Letters 2017年第10期28卷 2009-2013页

作者： Ai-min Luo Yongliang Shao Ke-jia Zhang Ya-Wen Wang yu Peng State Key Laboratory of Applied Organic Chemistry and College of Chemical Engineering Lanzhou University Lanzhou 730000 China

By modifying the salicylic-acid moiety with electron-withdrawing or –donating groups, three new terbium complexes（LⅠ·Tb, LⅡ·Tb, L·（Ⅲ）Tb） based on tripodal carboxylate ligands were synthesized. Due to different pull-push electronic effects of ligands, the fluorescence intensities of these terbium complexes significantly varied, that is： LⅡ·Tb 〉L（Ⅲ）·Tb〉 LⅠ·Tb. Meanwhile, the characteristic peaks at 492 nm（5D4→7F6） and 547 nm（5 D4→7F5） showed ＂Off–On–Off＂ fluorescence response to various p H conditions,which indicated that all of them can be used as the highly sensitive pH fluorescent probes. Notably, using LⅡ·Tb with the best fluorescence performance as a probe, some patients＇ urine samples can be easily monitored through the response triggered by pH value. Therefore, LⅡ·Tb has the potential to auxiliarily diagnose some diseases in clinical practice through p H detection of routine urine test.

关键词： Fluorescent probe Terbium complex pH Detection Ligand Routine urine test

Multi-dimensional Classification via Selective Feature Augmentation

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Machine Intelligence Research 2022年第1期19卷 38-51页

作者： Bin-Bin jia min-Ling Zhang School of Computer Science and Engineering Southeast UniversityNanjing 210096China College of Electrical and Information Engineering Lanzhou University of TechnologyLanzhou 730050China Key Laboratory of Computer Network and Information Integration Southeast UniversityMinistry of EducationChina

In multi-dimensional classification(MDC), the semantics of objects are characterized by multiple class spaces from different dimensions. Most MDC approaches try to explicitly model the dependencies among class spaces in output space. In contrast, the recently proposed feature augmentation strategy, which aims at manipulating feature space, has also been shown to be an effective solution for MDC. However, existing feature augmentation approaches only focus on designing holistic augmented features to be appended with the original features, while better generalization performance could be achieved by exploiting multiple kinds of augmented *** this paper, we propose the selective feature augmentation strategy that focuses on synergizing multiple kinds of augmented ***, by assuming that only part of the augmented features is pertinent and useful for each dimension′s model induction, we derive a classification model which can fully utilize the original features while conduct feature selection for the augmented features. To validate the effectiveness of the proposed strategy, we generate three kinds of simple augmented features based on standard k NN, weighted k NN, and maximum margin techniques, respectively. Comparative studies show that the proposed strategy achieves superior performance against both state-of-the-art MDC approaches and its degenerated versions with either kind of augmented features.

关键词： Machine learning multi-dimensional classification feature augmentation feature selection class dependencies

Global FLS-based Consensus of Stochastic Uncertain Nonlinear Multi-agent Systems

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International Journal of Automation and computing 2021年第5期18卷 826-837页

作者： jia-Xi Chen Jun-min Li School of Mathematics and Statistics Xidian UniversityXi'an 710071China

Using graph theory, matrix theory, adaptive control, fuzzy logic systems and other tools, this paper studies the leader-follower global consensus of two kinds of stochastic uncertain nonlinear multi-agent systems(MAS). Firstly, the fuzzy logic systems replaces the feedback compensator as the feedforward compensator to describe the uncertain nonlinear dynamics. Secondly, based on the network topology, all followers are divided into two categories: One is the followers who can obtain the leader signal, and the other is the follower who cannot obtain the leader signal. Thirdly, based on the adaptive control method, distributed control protocols are designed for the two types of followers. Fourthly, based on matrix theory and stochastic Lyapunov stability theory, the stability of the closed-loop systems is analyzed. Finally, three simulation examples are given to verify the effectiveness of the proposed control algorithms.

关键词： Fuzzy logic systems stochastic nonlinear multi-agent systems(MAS) consensus algorithm adaptive control

Saddle pulmonary embolism is not a sign of high-risk deterioration in non-high-risk patients: A propensity score-matched study

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World Journal of Emergency Medicine 2021年第4期12卷 261-267页

作者： Dong jia Chao Ji min Zhao Department of Emergency Medicine Shengjing Hospital of China Medical UniversityShenyang 110004China Department of Clinical Epidemiology Shengjing Hospital of China Medical UniversityShenyang 110004China

BACKGROUND: There is controversy regarding whether saddle main pulmonary artery(MPA) embolism represents a high risk of deterioration in non-high-risk acute pulmonary embolism(PE) patients. This study aims to address this issue by conducting a propensity score matching(PSM) ***: A total of 727 non-high-risk acute PE patients were retrospectively evaluated. We evaluated the Bova score and risk stratification to examine the risk of deterioration. Deterioration defined as any adverse event within 30 days after admission. Computed tomographic pulmonary angiography was used to identify the embolism type. All patients were matched into four subgroups by PSM according to age, sex, Bova score, and risk stratification:(1) MPA and non-MPA embolism;(2) non-saddle MPA and non-MPA embolism;(3) saddle MPA and non-saddle MPA embolism;(4) saddle MPA and non-MPA embolism. Correlations were analyzed using Cox regression analysis, and deterioration risk was compared between subgroups using Kaplan-Meier ***: Cox regression analysis revealed that MPA embolism was correlated with deterioration, regardless of whether saddle MPA embolism was included or excluded. Saddle MPA embolism was not correlated with deterioration, regardless of comparison with non-saddle MPA embolism or non-MPA embolism. Patients with MPA and non-saddle MPA embolism presented a high risk for deterioration(logrank test=5.23 and 4.70, P=0.022 and 0.030, respetively), while patients with saddle MPA embolism were not at a high risk of deterioration(log-rank test=1.20 and 3.17, P=0.729 and 0.077, respetively).CONCLUSIONS: Saddle MPA embolism is not indicative of a high risk of deterioration in nonhigh-risk acute PE patients.

关键词： Main pulmonary artery Pulmonary embolism Computerized tomography pulmonary arteriography