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Insect Science 2007年第3期14卷 241-249页

作者： HONG-JIAO CAI ZHI-sheng LI min-sheng you Institute of Applied Ecology Research Centre for Biodiversity and Eco-Safety Fujian Agriculture and Forestry University Fuzhou China

Field trials were carried out from June to August in 2004 at Wuyishan （Wuyi Mountains）, Fujian province, China, to determine the effects of habitat diversification on arthropod communities. Two Chinese cabbage, Brassica chinensis, field 1 （F1） and field 2 （F2） surrounded by diverse vegetable cultivars were selected, while a monoculture of Chinese cabbage served as the control field （CK）. The results showed that：（i） when comparing insect abundance of each order between different habitats, significantly higher numbers of lepidopterous insects （39.76% from the each order） and lower densities of Hymenoptera （19.82%） were found in CK than in F1 and F2; （ii） compared with CK, F1 and F2 had a lower percentage of species richness and an abundance of herbivorous insects, but increased richness, abundance and biodiversity of predatory insects; （iii） no differences were observed in neutral insects＇ guild between different fields; and （iv） the dominant species for each guild depends on the habitat types and sampling dates. This study suggests that intercropping could conceivably be used in these habitats to increase the population of natural predators, thus achieving desirable and ecologically friendly results in vegetable fields.

关键词： arthropod community community composition community architecture diversity habitat

Electroantennogram and behavioral responses of Cotesia plutellae to plant volatiles

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Insect Science 2016年第2期23卷 245-252页

作者： Guang Yang you-Nan Zhang Geoff M. Gurr Liette Vasseur min-sheng you Institute of Applied Ecology Fujian Agriculture and Forestry University Fuzhou China Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests Fujian Agriculture and Forestry University Fuzhou China Key Laboratory of Integrated Pest Management of Fujian and Taiwan China Ministry Agriculture Fuzhou Chin provincial Key Laboratory of Insect Ecology Fuzhou China Putian Institute of Agricultural Sciences Putian China Graham Centre Charles Sturt University & NSW Department of Primary Industries Orange New South Wales Australia Department of Biological Sciences Brock University 500 Glenridge Avenue St. Catharines Ontario Canada

Plant volatiles have been demonstrated to play an important role in regulat- ing the behavior of Cotesia plutellae, a major larval parasitoid of the diamondback moth （DBM）, Plutella xylostella, but little is currently known about the function of each volatile and their mixtures. We selected 13 volatiles of the DBM host plant, a cruciferous veg- etable, to study the electroantennogram （EAG） and behavioral responses of C. plutellae. EAG responses to each of the compounds generally increased with concentration. Strong EAG responses were to 100 μL/mL of trans-2-hexenal, benzaldehyde, nonanal and cis-3- hexenol, and 10/zL/mL of trans-2-hexenal and benzaldehyde with the strongest response provoked by trans-2-hexenal at 100μL/mL. In the Y-tube olfactometer, C. plutellae, was significantly attracted by 1μL/mL of trans-2-hexenal and benzaldehyde.β-caryophyllene, cis-3-hexenol or trans-2-hexenal significantly attracted C. plutellae at 10μL/mL, while nonanal, benzyl alcohol, cis-3-hexenol or benzyl cyanide at 100μL/mL significantly at- tracted C. plutellae. Trans-2-hexenal significantly repelled C. plutellae at 100 μL/mL. EAG of C. plutellae showed strong responses to all mixtures made of five various com- pounds with mixtures 3 （trans-2-hexenal, benzaldehyde, nonanal, cis-3-hexenol, benzyl cyanide, farnesene, eucalyptol） and 4 （trans-2-hexenal, benzaldehyde, benzyl alcohol, （R）- （＋）-limonene,β-ionone, farnesene, eucalyptol） significantly attracting C. plutellae. These findings demonstrate that the behavior of C. plutellae can be affected either by individual compounds or mixtures of plant volatiles, suggesting a potential of using plant volatiles to improve the efficiency of this parasitoid for biocontrol ofP. xylostella.

关键词： behavioral response Cotesia plutellae electroantennogram response herbivore-induced plant volatile

Higher taxa as surrogates of species richness of spiders in insect-resistant transgenic rice

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Insect Science 2012年第3期19卷 419-425页

作者： sheng Lin min-sheng you Liette Vasseur Guang Yang Feng-Jing Liu Feng Guo Institute of Applied Ecology and Research Centre for Biodiversity and Eco-Safety Fujian Agriculture and Forestry University Fuzhou China Department of Biological Sciences Brock University St. Catharines Ontario Canada

Biodiversity assessments can often be time- and resource-consuming. Several alternative approaches have been proposed to reduce sampling efforts, including indicator taxa and surrogates. In this study, we examine the reliability of higher taxon surrogates to predict species richness in two experimental rice fields of Fujian Province, southeastern China during 2005 and 2009. Spider samples in transgenic and nontransgenie plots were collected using a suction sampler. Both the genus and family surrogates had significant and positive linear relationships with species richness in the transgenic and nontransgenic rice fields. The rice varieties did not significantly influence the linear relationships. Our findings suggest that higher-taxon surrogacy could be a useful alternative to complete species inventory for risk assessments of transgenic rice.

关键词： Araneae biodiversity surrogate ecological risk assessment higher-taxonsurrogacy rapid biodiversity assessment taxonomic resolution

Cell Iines from diamondback moth exhibiting differential susceptibility to baculovirus infection and expressing midgut genes

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Insect Science 2019年第2期26卷 251-262页

作者： Xiao-Li Ma Wei-Yi He Ping Wang min-sheng you State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry University Fuzhou China Fujian -Taiwan Joint Centre for Ecological Control of Crop Pests Fujian Agriculture and Forestry University Fuzhou China Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops Ministry of Agriculture Fujian Agriculture and Forestry University Fuzhou China Institute of Applied Ecology Fujian Agriculture and Forestry University Fuzhou China Department of Entomology Cornell University Geneva NY USA

Abstract Six new cell lines were established from embryonic tissues of the diamondback moth, Plutella xylostella (L.). The cell lines showed differential characteristics, including growth in attachment or in suspension, susceptibility to a baculovirus infection and expression of genes involved in the glucosinolate detoxification pathway in R xylostella larvae. Five of the cell lines grew attached to the culture flask and one cell line grew unattached as a suspension cell line. The cell lines had population doubling times ranging from IS to 23 h. Among five of the P. xylostella cell lines examined for infection of a nucleopolyhe. drovirus from Autographa californica, AcMNPV four cell lines were highly susceptible to AcMNPV infection, but one was only semi-permissive to AcMNPV infection. The production of two recombinant proteins, a β-galactosidase of bacterial origin and a secreted alkaline phosphatase of eukaryotic origin, in the R xylostella cell lines was examined in comparison with that in the cell line Sf9 which is commonly used for recombinant protein production. In the P. xylostella cell lines, expression of three important midgut genes involved in the glucosinolate detoxification pathway, including the glucosinolate sulfatase genes GSS1 and GSS2 and the sulfatase modifying factor gene SUMF1、was detected. The R xylostella cell lines developed in this study could be useful in in vitro research systems for studying insec-virus interactions and complex molecular mechanisms in glucosinolate detoxification and insect-plant interactions.

关键词： AcMNPV cell lines glucosinolate sulfatase (GSS) Plutella xylostella PxGV sulfatase modifying factor 1 (SUMF1)

Identification and characterization of odorant binding proteins in the diamondback moth,Plutella xylostella

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Insect Science 2021年第4期28卷 987-1004页

作者： Li-Jun Cai Li-Shuang Zheng Yu-Ping Huang Wei Xu min-sheng you State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Institute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhouChina Joint International Research Laboratory of Ecological Pest Control Ministry of EducationFuzhouChina Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops Ministry of AgricultureFuzhouChina Agricultural Sciences College of ScienceHealthEngineering and EducationMurdoch UniversityMurdochAustralia

Odorant binding proteins(OBPs)are a group of soluble proteins functioning as odorant carriers in insect antennae,mouth parts and other chemosensory ***,multiple insect OBPs have been detected in other tissues and various functions have been ***,a detailed expression profile including stages,tissues and sexes where OBPs are expressed will assist in building the links to their potential functions,enhancing the functional studies of insect ***,we identified 39 putative OBP genes from its genome and transcriptome sequences of diamondback moth(DBM),Plutella *** expression patterns of identified PxylOBPs were further investigated from eggs,larvae,pupae,virgin adults,mated adults,larval midgut,larval heads,adult antennae,adult heads and adult ***,*** larvae and adults with and without host plants for 5 h were utilized to study the interactions between OBP expression and host *** results showed that most PxylOBPs were highly expressed in male and female adult *** expression levels of certain PxyOBPs could be regulated by mating activities and feeding host *** study advances our knowledge of *** OBPs,which may help develop new strategies for more environmentally sustainable management of ***.

关键词： gene expression insect olfaction OBP qRT-PCR

Structure and expression of sulfatase and sulfatase modifying factor genes in the diamondback moth,Plutella xylostella

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Insect Science 2018年第6期25卷 946-958页

作者： Xiao-Li Ma Wei-Yi He Wei Chen Xue-Jiao Xu Wei-Ping Qi ming-min Zou Yan-Chun you Simon W.Baxter Ping Wang min-sheng you State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry UniversityFuzhouChina. Institute of Applied Ecology Fujian Agriculture and Forestry UniversityFuzhouChina. Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests Fujian Agriculture and Forestry UniversityFuzhouChina. Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops Ministry of AgricultureFuzhouChina. Department of Entomology Comell UniversityNew York State Agricultural Experiment StationGenevaNYUSA . School of Biological Sciences University of AdelaideSouth AustraliaAustralia.

The diamondback moth,Plutella xylostella (L.),uses sulfatases (SULF)to counteract the glucosinolate-myrosinase defensive system that cruciferous plants have evolved to deter insect *** activity is regulated by post-translational modi- fication of a cysteine residue by sulfatase modifying factor 1(SUMF1).We identified 12 SULF genes (PxylSulfs)and two SUMF1 genes (PxylSumfls)in the *** genome. Phylogenetic analysis of SULFs and SUMFs from ***,Bombyx mori,Manduca sexta,Heliconius melpomene,Danaus plexippus,Drosophila melanogaster,Tetranychus urticae and Homo sapiens showed that the SULFs were clustered into five groups,and the SUMFs could be divided into two *** of the expression of PxylSulfs and Pxyl Sumfs by RNA-seq and by quantitative real-time polymerase chain reaction showed that two glucosinolate sulfatase genes (GSS),PxylSulf2and PxylSulf3,were primarily expressed in the midgut of 3rd-and 4th-instar ***,expression of sulfatases PxylSulf2, PxylSulf3 and PxylSulf4 were correlated with expression of the sulfatases modifying fac tor *** findings from this study provide new insights into the structure and expression of SUMF1and PxylSulf genes that are considered to be key factors for the evolutionary success *** as a specialist herbivore of cruciferous plants.

关键词： evolutionary arms race glucosinolate detoxification glucosinolate sulfatase phylogenetie analysis transcriptome analysis

Characterization of a myrosinase cDNA from Brassica parachinensis and its defense role against Plutella xylostella after suppression

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Insect Science 2012年第4期19卷 461-471页

作者： Guang Yang Ding-Feng wang Zhi-Quan Dong Qian-Liang Wang Gefu Wang-Pruski min-sheng you lnstitute of Applied Ecology Fujian Agriculture and Forestry UniversityFuzhou Tea Research Institute Fujian Academy of AgriculturalScienceFu'anFujian Institute of Prevention and Control of Professional Diseases FuzhouChina Department of Plant and AnimalSciences Nova Scotia Agricultural CollegeTruroNova ScotiaCanada

In Brassicaceae, myrosinase catalyzes the hydrolysis of glucosinolate and plays an important role in anti-herbivore defense. We have cloned and characterized the full- length complementary DNA of myrosinase gene from Brassicaparachinensis that exhibits high sequence identity with myrosinase genes from other Brassica species. To investigate the role of this myrosinase in defense against the diamondback moth （Plutella xylostella）, we constructed an RNA-interference （RNAi） cassette expressing a double-stranded RNA that targeted myrosinase and transfected it into B. parachinensis. Myrosinase was sup- pressed in the resulting transgenic plants. Diamondback moth larvae feeding on transgenic plants had lower larval and pupal weights, longer pupal duration, and lower fecundity than those feeding on non-transgenic plants, suggesting that the diamondback moth has adapted to the glucosinolate-myrosinase defensive system. Therefore, the suppression of myrosinase is a potential approach for controlling the diamondback moth.

关键词： Brassica parachinensis myrosinase Plutella xylostella RNA interference

Population characteristics of Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae) reared on a mushroom fly Coboldia fuscipes (Diptera: Scatopsidae)

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Insect Science 2019年第2期26卷 322-332页

作者： Mei-Fang Wen Hsin Chi Ying-Xiao Lian Yu-Hao Zheng Qing-Hai Fan min-sheng you State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry University Fuzhou China Institute of Applied Ecology Fujian Agriculture and Forestry University Fuzhou China Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests Fujian Agriculture and Forestry University Fuzhou China Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops Ministry of Agriculture Fuzhou China Department of Plant Production and Technologies Faculty of Agricultural Sciences and Technologies Omer Halisdemir University Nigde Turkey Plant Health & Environment Laboratory Ministry for Primary Industries Auckland New Zealand

Subterranean predatory mites are important biological control agents of pests in soil. In order to understand the population characteristics of two predatory mites, Macrocheles glaber Miiller and Stratiolaelaps scimitus Womersley, we studied their development, survival and fecundity data under laboratory conditions using Coboldia fuscipes Meigen as a food source and analyzed them with the age-stage, two-sex life table. Macrocheles glaber had a significantly shorter developmental time, oviposition period, longevity and lower fecundity than those of S. scimitus. The intrinsic rate of increase (λ), finite rate of increase (r), net reproductive rate (C0),net predation rate (C0), and finite predation rate (ω) of M. glaber were significantly lower than those of S. scimitus. Both population parameters and computer simulation implied that S. scimitus is a potential powerful biocontrol agent compared to M. glaber.

关键词： biocontrol life table predatory mites

Effects of nitrogen application on beetle communities in tea plantations

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Insect Science 2009年第2期16卷 185-192页

作者： Shao-Bo Chen Zhi-Juan Wei Zhao-Hua Zeng Li-Lin Chen Hui-Tao Chen min-sheng you Institute of Appfied Ecology Fujian Agriculture and Forestry University Fuzhou China

In contrast to grassland and forest ecosystems, little is known about insect response to nitrogen deposition in agricultural ecosystems. This study was carried out to investigate the effects of short-term （1-2 years） nitrogen application （0, 172.5, 345.0, 690.0, and 1035.0 kg · N ha^-1 · y^-1） on beetle communities in tea plantations. A collection of 34 families, 89 species of beetles, was obtained from a tea plantation in Wuyishan, China. Among them, herbivores, predators and detritivores had 52, 29, and eight species, respectively. Species richness, effective diversity and abundance （measured as the number of individuals and insect biomass） of the beetle community were not significantly related to the rate of nitrogen application. However, nitrogen application changed the species distribution and weakly increased the evenness of species distribution, while this did not significantly change the species evenness. Species richness and abundance of herbivores and predators were not significantly related to the rate of nitrogen application. However, there were some variations in trophic responses to nitrogen. Species richness and abundance of detritivores increased with increasing nitrogen application.

关键词： beetle community diversity abundance nitrogen application

Neighbor Sum Distinguishing Total Choosability of Planar Graphs with Maximum Degree at Least 10

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Acta Mathematicae Applicatae Sinica 2024年第1期40卷 211-224页

作者： Dong-han Zhang you Lu sheng-gui Zhang Li Zhang School of Mathematics and Statistics Northwestern Polytechnical UniversityXi’an710129China School of Mathematics and Computer Application Shangluo UniversityShangluo726000China

A neighbor sum distinguishing(NSD)total coloringφof G is a proper total coloring of G such thatΣz∈EG(u)U{u}φ(z)≠Σz∈EG(v)U{v}φ(z)for each edge uv∈E(G),where EG(u)is the set of edges incident with a vertex *** 2015,Pilśniak and Wozniak conjectured that every graph with maximum degreeΔhas an NSD total(Δ+3)-***,Yang et *** that the conjecture holds for planar graphs withΔ≥10,and Qu et *** that the list version of the conjecture also holds for planar graphs withΔ≥*** this paper,we improve their results and prove that the list version of the conjecture holds for planar graphs withΔ≥10.

关键词： planar graphs neighbor sum distinguishing total choosibility combinatorial nullstellensatz discharging method