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Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway

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Chinese Journal of Integrative Medicine 2024年第1期30卷 34-41页

作者： qi Zhi-min WANG Xia LIU Xia ZHAO Juan Medical Examination Center Hebei Province Hospital of Chinese MedicineShijiazhuang(050011)China Department of Gynaecology and Obstetrics Hebei Province Hospital of Chinese MedicineShijiazhuang(050011)China Experimental Center for Teaching Hebei Medical UniversityShijiazhuang(050017)China

Objective: To investigate the effects of asperuloside on cervical cancer based on endoplasmic reticulum(ER) stress and mitochondrial pathway. Methods: Different doses(12.5–800 μg/mL) of asperuloside were used to treat cervical cancer cell lines Hela and CaSki to calculate the half maximal inhibitory concentration(IC_(50)) of asperuloside. The cell proliferation was analyzed by clone formation assay. Cell apoptosis, intracellular reactive oxygen species(ROS) and mitochondrial membrane potential were determined by flow cytometry. The protein expressions of cleaved-caspase-3, Bcl-2, Bax, Cyt-c, cleaved-caspase-4 and glucose-regulated protein 78(GRP78) were analyzed by Western blot. And the inhibitor of ER stress, 4-phenyl butyric acid(4-PBA) was used to treat cervical cancer cells to further verify the role of ER stress in the apoptosis of cervical cancer cells induced by asperuloside. Results: Asperuloside of 325, 650, and 1300 μg/mL significantly inhibited the proliferation and promoted apoptosis of Hela and CaSki cells(P<0.01). All doses of asperuloside significantly increased intracellular ROS levels, reduced mitochondrial membrane potential, significantly reduced Bcl-2 protein expression level, and increased Bax, Cyt-c, GRP78 and cleaved-caspase-4 expressions(P<0.01). In addition, 10 mmol/L 4-PBA treatment significantly promoted cell proliferation and reduced apoptosis(P<0.05), and 650 μg/mL asperuloside could reverse 4-PBA-induced increased cell proliferation, decreased apoptosis and cleaved-caspase-3,-4 and GRP78 protein expressions(P<0.05). Conclusion: Our study revealed the role of asperuloside in cervical cancer, suggesting that asperuloside promotes apoptosis of cervical cancer cells through ER stress-mitochondrial pathway.

关键词： asperuloside Chinese medicine cervical cancer endoplasmic reticulum stress mitochondrial damage apoptosis

Zn-air batteries for electric vehicles

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Tungsten 2024年第1期6卷 164-173页

作者： Nai-qi Meng Yu-xin Fan Jing-Sheng Cai State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai 200438China College of Energy Soochow Institute for Energy and Materials InnovationS(SIEMIS)Key Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu Province Soochow UniversitySuzhou 215006China Institute of New Energy for Vehicles School of Materials Science and EngineeringTongji UniversityShanghai 201804China

The increasingly serious environmental challenges have gradually aroused people's interest in electric *** the last decade,governments and automakers have collaborated on the manufacturing of electric vehicles with high ***-edge battery technologies are pivotal for the performance of electric ***-air batteries are considered as potential power batteries for electric vehicles due to their high ***-air battery researches can be classified into three categories:primary batteries,mechanically rechargeable batteries,and chemically rechargeable *** majority of current studies aim at developing and improving chemically rechargeable and mechanically rechargeable Zn-air *** have tried to use catalytic materials design and device design for Zn-air batteries to make it possible for their applications in electric *** review will highlight the state-of-the-art in primary batteries,mechanically rechargeable batteries,and chemically rechargeable batteries,revealing the prospects of Zn-air batteries for electric vehicles.

关键词： Energy storage Electric vehicles Bifunctional oxygen catalysts Mechanically rechargeable Zn-air batteries Chemically rechargeable Zn-air batteries

Wind-sand flow simulation and radius optimization of highway embankment under different vertical curve radius

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Journal of Mountain Science 2024年第8期21卷 2533-2546页

作者： LI Liangying LI qi WANG Xu xin Guowei WANG Zhenqiang School of Civil Engineering Lanzhou Jiaotong UniversityLanzhou 730070China

It is of great practical value to explore the correlation between the vertical curve radius of desert highway and the increase of sand accumulation in local lines,and to select the appropriate vertical curve radius for reducing the risk of sand *** this study,three-dimensional models of desert highway embankments with different vertical curve radii were constructed,and Fluent software was used to simulate the wind-sand flow field and sand accumulation distribution of vertical curve *** results show that:(1)Along the direction of the road,the concave and the convex vertical curve embankments have the effect of collecting and diverging the wind-sand flow,*** the radius of the concave vertical curve is 3000 m,5000 m,8000 m,10000 m and 20000 m,the wind velocity in the middle of the vertical curve is 31.76%,22.58%,10.78%,10.53%and 10.44%,higher than that at both *** the radius of the convex vertical curve is 6500 m,8000 m,10000 m,20000 m and 30000 m,the wind velocity at both ends of the vertical curve is 14.06%,9.99%,6.14%,3.22%and 2.41%,higher than that in the *** diversion effect also decreases with the increase of the radius.(2)The conductivity of the concave and convex vertical curve embankments with different radii is greater than 1,which is the sediment transport *** conductivity increases with the increase of radius and gradually tends to be *** the radius of the concave and convex vertical curves reaches 8000 m and 20000 m respectively,the phenomenon of sand accumulation is no longer *** the same radius condition,the concave vertical curve embankment is more prone to sand accumulation than the convex one.(3)Considering the strength of the collection and diversion of the vertical curve embankment with different radii,and the sand accumulation of the vertical curve embankment in the desert section of Wuma Expressway,the radius of the concave vertical curve is not less than 8000 m,and the r

关键词： Highway engineering Vertical curve Numerical simulation Embankment wind-sand flow Radius optimization

Plant aquaporins:Their roles beyond water transport

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The Crop Journal 2024年第3期12卷 641-655页

作者： qi Sun xin Liu Yoshichika Kitagawa Giuseppe Calamita Xiaodong Ding Key Laboratory of Agricultural Biological Functional Genes Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Graduate School of Bioresource Sciences Akita Prefectural UniversityAkita 010-0195Japan Department of Biosciences Biotechnologies and BiopharmaceuticsUniversity of Bari‘Aldo Moro’I-70125 BariItaly Key Laboratory of Soybean Biology of Chinese Education ministry Northeast Agricultural UniversityHarbin 150030HeilongjiangChina

Compared to other organisms,plants have evolved a greater number of aquaporins with diverse substrates and functions to adapt to ever-changing environmental and internal stimuli for growth and *** aquaporins were initially identified as channels that allow water molecules to cross biological membranes,progress has been made in identifying various novel permeable *** studies have characterized the versatile physiological and biophysical functions of plant ***,we review the recent reports that highlight aquaporin-facilitated regulation of major physiological processes and stress tolerance throughout plant life cycles as well as the potential prospects and possibilities of applying aquaporins to improve agricultural productivity,food quality,environmental protection,and ecological conservation.

关键词： Plant aquaporin Substrate Physiological function Agriculture and environment

Copper Metabolism and Cuproptosis:Molecular Mechanisms and Therapeutic Perspectives in Neurodegenerative Diseases

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Current Medical Science 2024年第1期44卷 28-50页

作者： Xiao-xia BAN Hao WAN xin-xing WAN Ya-ting TAN Xi-min HU Hong-xia BAN xin-yu CHEN Kun HUANG qi ZHANG Kun XIONG Department of Human Anatomy and Neurobiology School of Basic Medical ScienceCentral South UniversityChangsha 430013China Department of Endocrinology Third Xiangya HospitalCentral South UniversityChangsha 430013China Department of Dermatology Xiangya HospitalCentral South UniversityChangsha 430013China Affiliated Hospital Inner Mongolia Medical UniversityHohhot 010050China Key Laboratory of Emergency and Trauma of ministry of Education Hainan Medical UniversityHaikou 571199China Hunan Key Laboratory of Ophthalmology Changsha 430013China

Copper is an essential trace element,and plays a vital role in numerous physiological processes within the human *** normal metabolism,the human body maintains copper *** deficiency or excess can adversely affect cellular ***,copper homeostasis is stringently *** studies suggest that copper can trigger a specific form of cell death,namely,cuproptosis,which is triggered by excessive levels of intracellular *** induces the aggregation of mitochondrial lipoylated proteins,and the loss of iron-sulfur cluster *** neurodegenerative diseases,the pathogenesis and progression of neurological disorders are linked to copper *** review summarizes the advances in copper homeostasis and cuproptosis in the nervous system and neurodegenerative *** offers research perspectives that provide new insights into the targeted treatment of neurodegenerative diseases based on cuproptosis.

关键词： cuproptosis copper metabolism copper homeostasis neurodegeneration neurodegenerativedisease

Temporal and spatial regulation of biomimetic vascularization in 3D-printed skeletal muscles

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Bio-Design and Manufacturing 2024年第5期7卷 597-610页

作者： minxuan Jia Tingting Fan Tan Jia xin Liu Heng Liu qi Gu Key Laboratory of Organ Regeneration and Reconstruction State Key Laboratory of Membrane BiologyInstitute of ZoologyChinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Department of Orthopaedics Beijing Jishuitan HospitalCapital Medical UniversityBeijing 100035China

In the intricate skeletal muscle tissue,the symbiotic relationship between myotubes and their supporting vasculature is pivotal in delivering essential oxygen and *** study explored the complex interplay between skeletal muscle and endothelial cells in the vascularization ofmuscle *** harnessing the capabilities of three-dimensional(3D)bioprinting and modeling,we developed a novel approach involving the co-construction of endothelial and muscle cells,followed by their subsequent *** findings highlight the importance of the interaction dynamics between these two cell ***,introducing endothelial cells during the advanced phases of muscle differentiation enhanced myotube ***,it stimulated the development of the vascular network,paving the way for the early stages of vascularized skeletal muscle *** methodology proposed in this study indicates the potential for constructing large-scale,physiologically aligned skeletal ***,it highlights the need for exploring the delicate equilibrium and mutual interactions between muscle and endothelial *** on the multicell-type interaction model,we can predict promising pathways for constructing even more intricate tissues or organs.

关键词： Skeletal muscle Vascularization 3D bioprinting Cell interaction

Nickel-catalyzed carbonylative four-component 1,4-dicarbofunctionalization of 1,3-enynes

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Science China Chemistry 2024年第6期67卷 2029-2038页

作者： Yan Zhao qi-Chao Shan Hong xin Pin Gao Xu Yang xin-Hua Duan Li-Na Guo Department of Chemistry School of ChemistryXi’an Key Laboratory of Sustainable Energy Material Chemistry and Engineering Research Center of Energy StorageMaterials and DevicesMinistry of EducationXi’an Jiaotong UniversityXi’an710049China School of Electrical Engineering Xi’an Jiaotong UniversityXi’an710049China

An efficient Ni-catalyzed four-component 1,4-carbocarbonylation of 1,3-enynes with activated alkyl halides and arylboronic acids under atmospheric pressure of CO is *** tuning the electronic and steric effects of alkyl radicals,both electronrich and electron-deficient 1,3-enynes were compatible with this *** protocol features mild conditions,broad substrate scope,excellent functional group compatibility and facile gram-scale synthesis,providing a practical approach to the quaternary carbon center-containing allenyl *** study revealed that the acyl-NiIIspecies plays an important role in both the coupling and the alkyl radical generation processes.

关键词： Ni catalysis four-component 1 4-carbocarbonylation 1 3-enynes

Treatment of multisystem inflammatory syndrome in children

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World Journal of Pediatrics 2024年第4期20卷 325-339页

作者： Tong Tong Yi-Hua Jin min Wang Fang-qi Gong Department of Cardiology Children's HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthNo.3333 Binsheng RoadHangzhou 310052China

Background Multisystem inflammatory syndrome in children(MIS-C),a relatively uncommon but severe pediatric complication,is associated with coronavirus disease 2019(COVID-19).A variety of treatment approaches,including intravenous immunoglobulins(IVIGs),glucocorticoids(GCs)and biologic agents,such as anakinra and infliximab,have been described for the management of COVID-19-related *** therapy is also ***,a well-developed treatment system has not been established,and many issues remain *** recently published articles related to the treatment of MIS-C have been ***,in this review,we identified relevant articles published recently and summarized the treatment of MIS-C more comprehensively and *** sources We reviewed the literature on the treatment of MIS-C through 20 September *** PubMed/Medline,Web of Science,EMBASE,and Cochrane Library databases were searched with the combination of the terms"multisystem inflammatory syndrome","MIS-C","PIMS-TS","therapy","treatment","drug","IVIG","GCs","intravenous immunoglobulin","corticosteroids","biological agent",and"aspirin".Results The severity of MIS-C varies,and different treatment schemes should be used according to the specific *** research and data collection are vital to better understand the pathophysiology and optimal management of *** MIS-Cis a disease involving multiple systems and has great *** the accumulation of additional experience,we have garnered fresh insights into its treatment ***,there remains a critical need for greater standardization in treatment protocols,alongside the pressing necessity for more robust and meticulously conducted studies to deepen our understanding of these protocols.

关键词： Coronavirus disease 2019-Corticosteroids Intravenous immunoglobulin Multisystem inflammatory syndrome in children-Treatment

Engineered interface of three-dimensional coralliform NiS/FeS_(2)heterostructures for robust electrocatalytic water cleavage

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Nano Research 2024年第6期17卷 4693-4701页

作者： xin Yu Jing Mei Yeshuang Du Xiaohong Cheng xing Wang qi Wu State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan 430073China Hubei Key Laboratory of Pollutant Analysis&Reuse Technology College of Chemistry and Chemical EngineeringHubei Normal UniversityHuangshi 435002China Hubei Key Laboratory of Low Dimensional Optoelectronic Materials and Devices Hubei University of Arts and ScienceXiangyang 441053China

Heterojunction structures improve the intrinsic activity of electrocatalysts by enhancing the charge transfer between the catalyst and the *** this paper,the NiS/FeS_(2)heterostructured electrocatalyst is fabricated by a simple sulfidation method using an interface engineering strategy to adjust the surface electron density of the *** expected,NiS/FeS_(2)electrocatalyst exhibits superior activity and durable oxygen evolution reaction(OER)stability,requiring only a low overpotential of 183 mV to achieve a current density of 10 mA·cm^(−2)and can be stable for more than 80 h,superior to NiS,FeS_(2)electrocatalyst individually,and precious RuO_(2).Notably,NiS/FeS_(2)is also a good bifunctional electrocatalyst with good overall water splitting performance,and it only requires a voltage 1.56 V to obtain a current density of 10 mA·cm^(−2)for more than 12 ***,the NiS/FeS_(2)hybridization facilitates the formation of coral-like structures,increasing the electrochemical surface area(ECSA)and enhancing the charge transfer efficiency,thus leading to excellent electrocatalytic *** work proposes a constructive strategy for designing efficient electrocatalysts based on interface engineering,and lays a foundation for designing a new class of electrocatalysts.

关键词： NiS/FeS_(2) interface engineering nanorods bifunctional water splitting