检索结果-南通市图书馆

Study of ss-DNA Adsorption and Nano-mechanical Properties on Mica Substrate with Surface Forces Apparatus

Chinese Journal of Mechanical Engineering 2018年第5期31卷 91-98页

作者： Gui-Bin Shen Ya-Jing Kan min-hua chen Yun-Fei chen Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments Southeast University Nanjing 211189 China School of Mechanical Engineering Southeast University Nanjing 211189 China

Many DNA?based devices need to build stable and controllable DNA films on surfaces. However, the most com?monly used method of film characterization, namely, the probe?like microscopes which may destroy the sample and substrate. Surface Forces Apparatus(SFA) technique, specializing in surface interaction studies, is introduced to investigate the e ects of DNA concentration on the formation of single?stranded DNA(ss?DNA) film. The result demonstrates that 50 ng/μL is the lowest concentration that ss?DNA construct a dense layer on mica. Besides, it is also indicated that at di erent DNA concentrations, ss?DNA exhibit diverse morphology: lying flat on surface at 50 ng/μL while forming bilayer or cross?link at 100 ng/μL, and these ss?DNA structures are stable enough due to the repeatabil?ity even under the load of 15 mN/m. At the same time, an obvious adhesion force is measured:/m at 100 ng/μL, respectively, which is attributed to the ion?correlation e ect. M-6.5 mN/m at 50 ng/μL and-5.3 mNoreover, the atomic force microscopy(AFM) images reveal the entire surface is covered with wormlike ss?DNA and the measured surface roughness(1.8±0.2 nm) also matches well with the film thickness by SFA. The desorption behaviors of ss?DNA layer from mica surface occur by adding sodium salt into gap bu er, which is mainly ascribed to the decreased ion?ion cor?relation force. This paper employing SFA and AFM techniques to characterize the DNA film with flexibility and stable mechanical ability achieved by ion bridging method, is helpful to fabricate the DNA?based devices in nanoscale.

关键词： Single?stranded DNA Adsorption Nano?mechanical properties Ion?correlation effect Surface forces apparatus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Adjuvant percutaneous radiofrequency ablation of feeding artery of hepatocellular carcinoma before treatment

引用

World Journal of Gastroenterology 2009年第21期15卷 2638-2643页

作者： Yi-Bin Hou min-hua chen Kun Yan Jin-Yu Wu Wei Yang Ultrasound Department Peking University School of Oncology Cancer Hospital & Institute No. 52 Fucheng Road Haidian District Beijing 100142 China

AIM:To evaluate the feasibility and efficacy of percutaneous radiofrequency ablation(RFA) of the feeding artery of hepatocellular carcinoma(HCC) in reducing the blood-flow-induced heat-sink effect of ***:A total of 154 HCC patients with 177 pathologically confirmed hypervascular lesions participated in the study and were randomly assigned into two ***-one patients with 75 HCCs(average tumor size,4.3 ± 1.1 cm) were included in group A,in which the feeding artery of HCC was identified by color Doppler flow imaging,and were ablated with multiple small overlapping RFA foci [percutaneous ablation of feeding artery(PAA)] before routine RFA treatment of the ***-three patients with 102 HCC(average tumor size,4.1 ± 1.0 cm) were included in group B,in which the tumors were treated routinely with ***-enhanced computed tomography was used as post-RFA imaging,when patients were followed-up for 1,3 and 6 ***:In group A,feeding arteries were blocked in 66(88%) HCC lesions,and the size of arteries decreased in nine(12%).The average number of punctures per HCC was 2.76 ± 1.12 in group A,and 3.36 ± 1.60 in group B(P = 0.01).The tumor necrosis rate at 1 mo post-RFA was 90.67%(68/75 lesions) in group A and 90.20%(92/102 lesions) in group *** recur-rence rate at 6 mo post-RFA was 17.33%(13/75) in group A and 31.37%(32/102) in group B(P = 0.04).CONCLUSION:PAA blocked effectively the feeding artery of *** of PAA and RFA significantly decreased post-RFA recurrence and provided an alternative treatment for hypervascular HCC.

关键词： Hepatocellular carcinoma Feeding artery Radiofrequency ablation Recurrence Color Dopplerflow imaging

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Radiofrequency Ablation for Postoperative Recurrences of Intrahepatic Cholangiocarcinoma

引用

Chinese Journal of Cancer Research 2011年第4期23卷 295-300页

作者： Ying Fu Wei Yang Wei Wu Kun Yan Bao-cai Xing min-hua chen Key Laboratory of Carcinogenesis and Translational Research (ministry of Education) 1Department of Ultrasound Department of Surgery Peking University School of Oncology Beijing Cancer Hospital & Institute Beijing 100142 China

Objective: Most recurrent intrahepatic cholangiocarcinoma (RICC) lost the opportunity of radical resection while most nonsurgical management failed to prolong patients’ survival. The efficacy and safety of radiofrequency ablation (RFA) as a local treatment for recurrent hepatocellular carcinoma have been confirmed by many clinical studies. The purpose of this study was to evaluate the efficacy, long-term survival and complications of RFA for RICC. Methods: A total of 12 patients with 19 RICCs after radical resection were included in this study. The tumors were 1.9–6.8 cm at the maximum diameter (median, 3.2±1.6 cm). All patients were treated with ultrasound guided RFA. There were two RFA approaches including percutaneous and open. Results: A total of 18 RFA treatment sessions were performed. Ablation was successful (evaluated by 1-month CT after the initial RFA procedure) in 18 (94.7%) of 19 tumors. By a median follow-up period of 29.9 months after RFA, 5 patients received repeated RFA because of intrahepatic lesion recurrence. The median local recurrence-free survival period and median event-free survival period after RFA were 21.0 months and 13.0 months, respectively. The median overall survival was 30 months, and the 1- and 3-year survival rates were 87.5% and 37.5%, respectively. The complication rate was 5.6% (1/18 sessions). The only one major complication was pleural effusion requiring thoracentesis. Conclusion: This study showed RFA may effectively and safely manage RICC with 3-year survival of 37.5%. It provides a treatment option for these RICC patients who lost chance for surgery.

关键词： Intrahepatic cholangiocarcinoma Hepatectomy Recurrence Radiofrequency ablation Survival

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Role of Contrast Enhanced Ultrasound in Radiofrequency Ablation of Metastatic Liver Carcinoma

引用

Chinese Journal of Cancer Research 2012年第1期24卷 44-51页

作者： Jin-yu Wu min-hua chen Wei Yang Shu-zhi Lin Wei Wu Shan-shan Yin HuiZhang Kun Yan Department of Ultrasound Harbin First HospitalHarbin 150010China Key Laboratory of Carcinogenesis and Translational Research(ministry of Education) Department of UltrasoundPeking University School of OncologyBeijing Cancer Hospital&InstituteBeijing 100142China

Objective： To investigate the application of contrast enhanced ultrasound （CEUS） in planning and guiding for radiofrequency ablation （RFA） for metastatic liver carcinoma （MLC）. Methods： One hundred and thirty-five patients with clinically and pathologically diagnosed MLC （from gastrointestinal tumors） were included in the present study, and 104 of them had received CEUS prior to RFA to assess the number, size, shape, infiltration, location and enhancing features of the lesions. Among the 204 patients, 21 （20.1%） were excluded from RFA treatment due to too many lesions or large infiltrative range based on CEUS. The remaining 83 patients with 147 lesions underwent RFA （group A）. During the same period, other 32 patients with 202 lesions serving as control group were treated based on findings of conventional ultrasound without contrast （group B）. The patients underwent follow-up enhanced CT at the 1st month, and then every 3-6 months after RFA. The tumor was considered as early necrosis if no contrast enhancement was detected in the treated area on the CT scan at the 2st month. Results： In group A, 72 of 147 MLC lesions （48.9%） showed increased sizes on CEUS. Among them, 48 lesions （66.6%） appeared enlarged in arterial phase, and 24 （33.3%） showed enlarged hypoechoic area in parenchymal phase. CEUS showed total 61 additional lesions in 35 patients （42.2%）（ranged from 8 to 15 mm） compared with conventional ultrasound （US）, and 42 （68.8%） of them were visualized in parenchymal phase only. There were total 208 lesions in group A underwent RFA with CEUS planning, and the tumor necrosis rate was 94.2% （196/208）. In this group, local recurrence was found in 26 lesions （7.7%） during 3-42 months＇ following up, and new metastases were seen in 30 cases （36.2%）. For group B, the tumor necrosis rate was 86.3% （88/202）, local recurrence in 27 lesions （16.7%）, and new metastases in 13 cases （41.9%）. Tumor early necrosis and recurrence rates were significantly different between th

关键词： Contrast enhanced ultrasound (CEUS) Liver metastasis Radiofrequency ablation (RFA)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Treatment efficacy of radiof requency ablation of 338 patients with hepatic malignant tumor and the relevant complications

引用

World Journal of Gastroenterology 2005年第40期11卷 6395-6401页

作者： min-hua chen Wei Yang Kun Yan Wen Gao Ying Dai Yan-Bin Wang Xiao-Peng Zhang Shan-Shan Yin Departmentof Ultrasound Peking University School of Oncology 52 Fu-cheng Rd Haidian District Beijing 100036 China

AIM： To investigate the treatment efficacy of radiofrequency ablation （RFA） of hepatic malignant tumor and the relevant complications. METHODS： A total of 338 patients with 763 hepatic tumors underwent ultrasound-guided RFA （565 procedures）. There were 204 cases of hepatic cellular carcinoma （HCC） with 430 tumors, the mean largest diameter was 4.0 cm. Of them, 48 patients （23.5%） were in stages Ⅰ-Ⅱ （UICC Systems） and 156 （76.5%） in stages Ⅲ-Ⅳ There were 134 cases of metastatic liver carcinoma （MLC）, with 333 metastases in the liver, the mean diameter was 4.1 cm, the liver metastases of 96 patients （71.6%） came from gastrointestinal tract. Ninety-three percent of the 338 patients were treated using the relatively standard protocol. Crucial attention must be paid to monitor the abnormal changes in ultrasound images as well as the vital signs of the patients to find the possible hemorrhage and peripheral structures injury in time. The tumors were considered as ablated completely, if no viability was found on enhanced CT within 24 h or at 1 mo after RFA. These patients were followed up for 3-57 too. RESULTS： The ablation success rate was 93.3% （401/430 tumors） for HCC and was 96.7% （322/333 tumors） for MLC. The local recurrence rate for HCC and MLC was 7.9% （34/430 tumors） and 10.5% （351333 tumors）, respectively. A total of 137 patients （40.5%） underwent 2-11 times of repeated ablations because of tumor recurrence or metastasis. The 1st, 2nd, and 3rd year survival rate was 84.6%, 66.6%, and 63.1%, respectively; the survival rate from 48 patients of I-II stage HCC was 93.7%, 80.4%, and 80.4%, respectively. The major complication rate in this study was 2.5% （14 of 565 procedures）, which consisted of 5 hemorrhages, i colon perforation, 5 injuries of adjacent structures, 2 bile leakages, and 1 skin burn. CONCLUSION： RFA, as a minimally invasive local treatment, has become an effective and relatively safe alternativefor the patients of hepatic malignant tumor, even of advanc

关键词： Radiofrequency ablation Liver neoplasms Survival Complication Ultrasonography

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Differentiation of atypical hepatic hemangioma from liver metastases: Diagnostic performance of a novel type of color contrast enhanced ultrasound

引用

World Journal of Gastroenterology 2020年第9期26卷 960-972页

作者： Xiao-Feng Wu Xiu-Mei Bai Wei Yang Yu Sun Hong Wang Wei Wu, min-hua chen Kun Yan Key Laboratory of Carcinogenesis and Translational Research(ministry of Education/Beijing) Department of UltrasoundPeking University Cancer Hospital&InstituteBeijing 100142China Key laboratory of Carcinogenesis and Translational Research(ministry of Education/Beijing) Department of PathologyPeking University Cancer Hospital&InstituteBeijing 100142China

BACKGROUND In clinical practice,the diagnosis is sometimes difficult with contrast-enhanced ultrasound(CEUS)when the case has an atypical perfusion *** parametric imaging(CPI)is an analysis software for CEUS with better detection of temporal differences in CEUS imaging using arbitrary *** measures the differences in arrival time of the contrast agent in lesions so that the perfusion features of atypical hemangioma and colorectal cancer(CRC)liver metastasis can be *** To evaluate the role of a novel type of CPI of CEUS in the differential diagnosis of atypical hemangioma from liver metastases in patients with a history of *** From January 2016 to July 2018,42 patients including 20 cases of atypical hemangioma and 22 cases of liver metastases from CRC were *** patients had a mean age of 60.5±9.3 years(range:39-75 years).All patients received ultrasound,CEUS and CPI *** and staff radiologists independently and retrospectively reviewed CEUS and CPI *** sets of criteria were assigned:(1)Routine CEUS alone;and(2)CEUS and *** diagnostic sensitivity,specificity,accuracy and receiver operating characteristic(ROC)curve of resident and staff radiologists were *** The following CPI features were significantly different between liver hemangioma and liver metastases analyzed by staff and resident radiologists:Peripheral nodular enhancement(65%-70.0%vs 4.5%-13.6%,P<0.001,P=0.001),mosaic/chaotic enhancement(5%-10%vs 68.2%-63.6%,P<0.001,P<0.001)and feeding artery(20%vs 59.1%-54.5%,P=0.010,P=0.021).CPI imaging offered significant improvements in detection rates compared with routine CEUS in both resident and staff *** resident radiologists,the specificity and accuracy of CEUS+CPI were significantly increased compared with that of CEUS(77.3%vs 45.5%,P=0.030;78.6%vs 50.0%,P=0.006).In addition,the area under the curve(AUC)of CEUS+CPI was significantly higher than that of CEUS(0.803 vs 0.757,

关键词： Color parametric imaging Contrast enhanced ultrasound Liver hemangioma Liver metastases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Radiofrequency ablation of hepatocellular carcinoma in difficult locations:Strategies and long-term outcomes

引用

World Journal of Gastroenterology 2015年第5期21卷 1554-1566页

作者： Wei Yang Kun Yan Gong-Xiong Wu Wei Wu Ying Fu Jung-Chieh Lee Zhong-Yi Zhang Song Wang min-hua chen Department of Ultrasound Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)Peking University Cancer Hospital and Institute Research Division Joslin Diabetes CenterHarvard Medical SchoolBostonMA 02215United States Department of Cardiovascular The Second Hospital Affiliated to Guangzhou Medical UniversityGuangzhou Institute of Cardiovascular Disease

AIM:To investigate the treatment strategies and longterm outcomes of radiofrequency ablation(RFA) of hepatocellular carcinoma(HCC) in difficult locations and to compare the results with non-difficult ***:From 2004 to 2012,a total of 470 HCC patients underwent ultrasound-guided percutaneous *** these HCC patients,382 with tumors located ≤ 5 mm from a major vessel/bile duct(n = 87),from peripheral important structures(n = 232) or from the liver capsule(n = 63) were regarded as difficult *** were 331 male patients and 51 female patients,with an average age of 55.3 ± 10.1 years old.A total of 235 and 147 patients had ChildPugh class A and class B liver function,*** average tumor size was 3.4 ± 1.2 *** treatment strategies were developed to treat these difficult *** the same period,88 HCC patients with tumors that were not in difficult locations served as the control *** the control group,74 patients were male,and 14 patients were female,with an average age of 57.4 ± 11.8 years *** these,62 patients and 26 patients had Child-Pugh class A and class B liver function,*** follow-up after RFA was performed to assess treatment *** results were generated from Kaplan-Meier estimates,and multivariate analysis was performed using the Cox regression ***:Early tumor necrosis rate in the difficult group was similar to that in the control group(97.6% vs 94.3%,P = 0.080).The complication rate in the difficult group was significantly higher than that in thecontrol group(4.9% vs 0.8%,P = 0.041).The followup period ranged from 6 to 116 mo,with an average of 28 ± 22.4 *** progression rate in the difficult group was significantly higher than that in the control group(12.7% vs 7.1%,P = 0.046).However,the 1-,3-,5-,and 7-year overall survival rates in the difficult group were not significantly different from those in the control group(84.3%,54.4%,41.2%,and 29.9% vs 92.5%,60.3%,43.2%,and

关键词： Radiofrequency ablation Ultrasound guidance Hepato

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ten-year survival of hepatocellular carcinoma patients undergoing radiofrequency ablation as a first-line treatment

引用

World Journal of Gastroenterology 2016年第10期22卷 2993-3005页

作者： Wei Yang Kun Yan S Nahum Goldberg Muneeb Ahmed Jung-Chieh Lee Wei Wu Zhong-Yi Zhang Song Wang min-hua chen Key Laboratory of Carcinogenesis and Translational Research (ministry of Education) Department of UltrasoundPeking University Cancer Hospital and Institute Division of Image-guided Therapy Department of RadiologyHadassah Hebrew University Medical Center Laboratory for minimally Invasive Tumor Therapies Department of RadiologyBeth Israel Deaconess Medical Center/Harvard Medical School

AIM: To investigate the long-term survival and prognostic factors in hepatocellular carcinoma (HCC) patients undergoing radiofrequency ablation (RFA) as a first-line ***: From 2000 to 2013, 316 consecutive patients with 404 HCC (1.0-5.0 cm; mean: 3.2 ± 1.1 cm) underwent ultrasonography-guided percutaneous RFA as a first-line treatment. There were 250 males and 66 females with an average age of 60.1 ± 10.8 years (24-87 years). Patients were followed for 1 year to > 10 years after RFA (234, 181, 136, and 71 for 3, 5, 7, and 10 years, respectively). Overall local response rates and long-term survival rates were assessed. Survival results were generated using Kaplan-Meier estimates, and multivariate analysis was performed using the Cox regression ***: In total, 548 RFA sessions were performed and major complications occurred in 10 sessions (1.8%). Local tumor progression and/or new tumor development were observed in 43.3% (132/305) of the patients during the follow-up period. Overall 5- and 10-year survival rates were 49.7% and 28.4%, respectively. Based on multivariate analysis, three factors were identified as independent prognostic factors for overall survival: Child-Pugh classification (HR = 4.054, P < 0.001), portal vein hypertension (HR = 2.743, P = 0.002), and tumor number (HR = 2.693, P = 0.003). The local progression-free 5- and 10-year survival rates were 42.7% and 19.5%. In addition to the Child-Pugh classification and the number of tumors, the number of RFA sessions (HR = 1.550, P = 0.002) was associated with local progression-free ***: RFA can achieve acceptable outcomes for HCC patients as a first-line treatment, especially for patients with Child-Pugh class A, patients with a single tumor and patients without portal vein hypertension.

关键词： Radiofrequency ablation Hepatocellular carcinoma Percutaneous Ultrasonography-guided Long term survival

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Activation of γ-aminobutyric Acid （A） Receptor Protects Hippocampus from Intense Exercise-induced Synapses Damage and Apoptosis in Rats

引用

Chinese Medical Journal 2015年第17期128卷 2330-2339页

作者： Yi Ding Lan Xie Cun-Qing Chang Zhi-min chen hua Ai Institution of Sports Medicine Peking University Third Hospital Beijing 100191 China

Background： Our previous study has confirmed that one bout of exhaustion （Ex） can cause hippocampus neurocyte damage, excessive apoptosis, and dysfunction, its initial reason is intracellular calcium overload in hippocampus triggered by N-methyl-D-aspartic acid receptor （NMDAR） over-activation. NMDAR activation can be suppressed by y-aminobutyric acid （A） receptor （GABAAR）. Whether GABAAR can prevent intense exercise-induced hippocampus apoptosis, damage, or dysfunction will be studied in this study. Methods： According to dose test, rats were randomly divided into control （Con）, Ex, muscimol （MUS, 0.1 mg/kg） and bicuculline （BIC, 0.5 mg/kg） groups, then all rats underwent once swimming Ex except ones in Con group only underwent training, lntracellular free calcium concentration （[Ca2＋]i） was measured by Fura-2-acetoxymethyl ester; glial fibrillary acidic protein （GFAP） and synaptophysin （SYP） immunofluorescence were also performed; apoptosis were displayed by dUTP nick end labeling （TUNEL） stain; endoplasmic reticulum stress-induced apoptosis pathway was detected by Western blotting analysis; Morris water maze was used to detect learning ability and spatial memory. Results： The appropriate dose was 0.1 mg/kg for MUS and 0.5 mg/kg for BIC. Ex group showed significantly increased [Ca^2＋]i and astrogliosis; TUNEL positive cells and levels of GFAP, B cell lymphoma-2 （Bcl-2） associated X protein （Bax）, caspase-3, caspase-12 cleavage, CCAAT/enhancer binding protein homologous protein （CHOP）, and p-Jun amino-terminal kinase （p-JNK） in Ex group also raised significantly compared to Con group, while SYP, synapse plasticity, and Bcl-2 levels in Ex group were significantly lower than those in Con group. These indexes were back to normal in MUS group. BIC group had the highest levels of [Ca^2＋]i, astrogliosis, TUNEL positive cell, GFAP, Bax, caspase-3, caspase-12 cleavage, CHOP, and p-JNK, it also gained the lowest SYP, synapse plasticity, and Bcl-2 levels among all groups. Wat

关键词： Endoplasmic Reticulum Stress-induced Apoptosis γ-aminobutyric Acid (A) Receptor Hippocampus Intense Exercise Synapse Plasticity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Progressive transformation of immortalized esophageal epithelial cells

引用

World Journal of Gastroenterology 2002年第6期8卷 976-981页

作者： Zhong-Ying Shen Li-Yan Xu min-hua chen Jian Shen WeiJia Cai,Department of Tumor Pathology Medical College of Shantou University,Shantou 515031,Guangdong Province,China Yi Zeng,Institute of Virology,Chinese Academy of Preventive Medicine.Beijing 100052,China Department of Tumor Pathology Medical College of Shantou University Guandong Province China. zhongyingshen@***

AIM: To investigate the progressive transformation of immortal cells of human fetal esophageal epithelium induced by human papillomavirus, and to examine biological criteria of sequential passage of cells, including cellular phenotype, proliferative rate, telomerase, chromosome and ***: The SHEE cell series consisted of immortalized embryonic esophageal epithelium which was in malignant transformation when cultivated over sixty passages without co-carcinogens. Cells of the 10th, 31st, 60th and 85th passages were present in progressive development after being transfected with HPV. Cells were cultivated in a culture flask and 24-hole cultural plates. Progressive changes of morphology, cell growth, contact-inhibition, and anchoragedependent growth characteristics were examined by phase contrast microscopy. The cell proliferation rate was assayed by flow cytometry. The modal number of chromosomes was analyzed. HPV18E6E7 was detected by Western blot methods and activities of telomerase were analyzed by *** of cells was detected with soft agar plates cultivated and with tumor formation in SCID ***: In morphological examination the 10th passage cells were in good differentiation, the 60th and 85th passages cells were in relatively poor differentiation, and the 31st passage cells had two distinct differentiations. The characteristics of the 85th and 60th passage cells were weakened at contact-inhibition and anchorage-dependent growth. Karyotypes of four stages of cells belonged to hyperdiploid or hypotriploid, and bimodal distribution of chromosomes appeared in the 31st and 60th passage cells. All of these characteristics combined with a increasing trend. The activities of telomerase were expressed in the latter three passages. Four fourths of SCID mice in the 85th passage cells and one fourth of SCID mice in the 60th passage cells developed tumors, but the cells in the 10th and 31st passage displayed no tumor ***

关键词：人胚食管上皮细胞进行性转化细胞遗传学变化端粒酶肿瘤发生

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：