检索结果-南通市图书馆

Journal of Molecular Cell Biology 2014年第6期8卷 458-472页

作者： Shu-Yi Wei Ting-Er Lin Wei-Li Wang Pei-Ling Lee min-chien tsai Jeng-Jiann Chiu Institute of Cellular and System Medicine 'National' Health Research Institutes Miaoli 350 Department of Physiology and Biophysics 'National' Defense Medical Center Taipei 114 Institute of Biomedical Engineering 'National' Tsing Hua University Hsinchu 300 Institute of Biomedical Engineering 'National' Cheng Kung University Tainan 701

T-tym phocyte migration under flow is critical for immune responses, but the mechanisms by which flow modulates the migratory beha- viors of T-lymphocytes remain unclear. Human peripheral blood T-lymphocytes （PBTLs）, when stimulated with phorboL 12-myristate 13-acetate （PMA）, stretched their ceU bodies dramatically and moved alongthe flow direction. In contrast, stromal ceil-derived factor- lα-stimulated PBTI.s deformed and migrated in a random manner. Here we elucidated the molecular mechanisms underlying flow- induced directionality and deformation of PMA-stimulated PBTLs. PMA primed PBTLs for polarization under flow, with protein kinase C （PKC）-δ enriched in the leading edge, PKC-β1 in the microtubuie organizing center, and PKC-1311 in the uropod and peripheral region. PKC-δ regulated cell protrusions in the leading edge through Tiaml/Racl/caLmoduUn, whereas PKC-β regulated RhoA/Rho- associated kinase activity and microtubule stability to modulate uropod contractility and detachment. Our findings indicate that PKC-δ and -β coordinate in the cell Leading edge and uropod, respectively, to modu｜ate the directionality and deformability of migratory T-Lymphocytes under flow.

关键词： deformability directionality migration PKCs T-lymphocyte

Shear stress induces synthetic-to-contractile phenotypic modulation in smooth muscle cells via PPAR-alpha/delta activations by prostacyclin released by sheared endothelial cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Shear stress induces synthetic-to-contractile phenotypic mod...

Assessment and Visualization of Ki67 Heterogeneity in Breast Cancers through Digital Image Analysis

第七届海峡两岸心血管科学研讨会

作者： min-chien tsai Shu chien Jeng-Jiann Chiu Division of Medical Engineering Research National Health Research InstitutesMiaoliTaiwan

Phenotypic modulation of smooth muscle cells(SMCs),which are located in close proximity to endothelial cells(ECs),is critical in regulating vascular *** investigated the role of flow-induced shear stress on ECs in modulating SMC phenotype and its underlying mechanism,using our newly developed EC/SMC co-culture flow system in which ECs and SMCs were separated by a porous *** of shear stress(12 dynes/ cm)to EC/SMC modulated SMC phenotype from synthetic to contractile state,with up-regulation of contractile markers,down-regulation of pro-inflammatory genes,and decreased percentage of cells in the synthetic phase. Treating SMCs with media from sheared ECs induced peroxisome proliferator-activated receptor(PPAR)-α,-δ, and-γligand binding activities;transfecting SMCs with specific small interfering RNAs(siRNAs)of PPAR-αand-δ, but not-γ,inhibited shear-induction of contractile *** exposed to shear stress released prostacyclin (PGI).Transfecting ECs with PGI synthase-specific siRNA inhibited shear-induced activation of PPAR-α/δ,up-regulation of contractile markers,down-regulation of pro-inflammatory genes,and decrease in percentage of SMCs in synthetic *** with PPAR-αdeficiency showed altered SMC phenotype toward a synthetic state,with increased arterial contractility in response to angiotensinⅡ,as compared with control *** results indicate that laminar shear stress induces synthetic-to-contractile phenotypic modulation in SMCs through the activation of PPAR-α/δby the EC-released *** findings provide insights into the mechanisms underlying the interplays of ECs with SMCs and the protective homeostatic function of laminar shear stress in modulating SMC phenotype.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Advances in Breast Cancer Research 2024年第2期13卷 11-26页

作者： chien-Hui Wu min-Hsiang Chang Hsin-Hsiu tsai Yi-Ting Peng Department of Anatomical Pathology Far Eastern Memorial Hospital New Taipei AI Lab Quanta Computer Inc. Taoyuan

The Ki67 index (KI) is a standard clinical marker for tumor proliferation;however, its application is hindered by intratumoral heterogeneity. In this study, we used digital image analysis to comprehensively analyze Ki67 heterogeneity and distribution patterns in breast carcinoma. Using Smart Pathology software, we digitized and analyzed 42 excised breast carcinoma Ki67 slides. Boxplots, histograms, and heat maps were generated to illustrate the KI distribution. We found that 30% of cases (13/42) exhibited discrepancies between global and hotspot KI when using a 14% KI threshold for classification. Patients with higher global or hotspot KI values displayed greater heterogenicity. Ki67 distribution patterns were categorized as randomly distributed (52%, 22/42), peripheral (43%, 18/42), and centered (5%, 2/42). Our sampling simulator indicated analyzing more than 10 high-power fields was typically required to accurately estimate global KI, with sampling size being correlated with heterogeneity. In conclusion, using digital image analysis in whole-slide images allows for comprehensive Ki67 profile assessment, shedding light on heterogeneity and distribution patterns. This spatial information can facilitate KI surveys of breast cancer and other malignancies.

关键词： Ki67 Heterogeneity Breast Cancer Digital Image Analysis

Effect of nocturia in patients with different severity of obstructive sleep apnea on polysomnography: A retrospective observational study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Urology 2024年第3期11卷 486-496页

作者： Chin-Heng Lu Hung-min Chang Kuang-Hsi Chang Yen-Chuan Ou Chao-Yu Hsu min-Che Tung Frank Cheau-Feng Lin Stella Chin-Shaw tsai Division of Urology Department of SurgeryTungs’Taichung Metroharbor HospitalTaichungTaiwanChina Rong Hsing Research Center for Translational Medicine Chung Hsing UniversityTaichungTaiwanChina College of Life Sciences Chung Hsing UniversityTaichungTaiwanChina Department of Otolaryngology Tungs’Taichung Metroharbor HospitalTaichungTaiwanChina Department of Medical Research Tungs’Taichung Metroharbor HospitalTaichungTaiwanChina General Education Center Jen-Teh Junior College of MedicineNursing and ManagementMiaoliTaiwanChina Graduate Institute of Biomedical Sciences China Medical UniversityTaichungTaiwanChina Department of Post-Baccalaureate Medicine College of MedicineChung Hsing UniversityTaichungTaiwanChina Department of Nursing Jen-Teh Junior College of MedicineNursing and ManagementMiaoliTaiwanChina Department of Surgery Chung Shan Medical UniversityTaichungTaiwanChina College of Medicine Chung Shan Medical UniversityTaichungTaiwanChina

Patients with suspected OSA were examined using *** were divided into two groups based on the presence of *** was defined as a patient who needed to void at least *** index(AHI)was employed to classify patients according to degrees of severity:AHIminimum arterial blood oxygenation saturation(p=0.046),and decreased AHI of non-rapid eye movement(p=0.047),AHI of total sleep time(p=0.010),and desaturation index(p=0.012).In moderate OSA patients,nocturia related to increased age(p<0.001),awake time(p=0.025),stage 1 sleep(p=0.033),and sleep latency(p=0.033),and decreased height(p=0.044),weight(p=0.025),and sleep efficiency(p=0.003).In severe OSA patients,nocturia related to increased weight(p=0.011),body mass index(p=0.009),awake time(p=0.008),stage 1 sleep(p=0.040),arousal number(p=0.030),arousal index(p=0.013),periodic limb movement number(p=0.013),and periodic limb movement index(p=0.004),and decreased baseline arterial blood oxygenation saturation(p=0.046).Conclusion:Our study revealed that there were more correlated factors related to nocturia with increasing severity of *** study helps in clinical education and treatment for OSA patients with different severity.

关键词： Nocturia Obstructive sleep apnea Polysomnography Apneaehypopnea index International Prostate Symptom Score

New Second-Level-Discrete Zeroing Neural Network for Solving Dynamic Linear System

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

IEEE/CAA Journal of Automatica Sinica 2024年第6期11卷 1521-1523页

作者： min Yang School of Robotics Hunan UniversityChangsha 410082China

Dear Editor,This letter deals with a new second-level-discretization method with higher precision than the traditional first-level-discretization ***,the traditional discretization method utilizes the first-order time derivative information,and it is termed first-level-discretization *** contrast,the new discretization method not only utilizes the first-order time derivative information,but also makes use of the second-order derivative *** combining the new second-level-discretization method with zeroing neural network(ZNN),the second-level-discrete ZNN(SLDZNN)model is proposed to solve dynamic(i.e.,time-variant or time-dependent)linear *** experiments and application to angle-of-arrival(AoA)localization show the effectiveness and superiority of the SLDZNN model.

关键词： method derivative utilize

Spinal intradural electrodes: opportunities, challenges and translation to the clinic

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第3期19卷 503-504页

作者： Bruce Harland chien Yew Kow Darren Svirskis School of Pharmacy University of AucklandAucklandNew Zealand Department of Neurosurgery Auckland City HospitalAucklandNew Zealand

Damage to the spinal cord disrupts the electrically active nerve cells which normally transmit afferent and efferent signals,resulting in loss of motor,sensory,and autonomic *** treatments for spinal cord injury utilizing implanted spinal electrodes can be broadly classified into three different *** first of these approaches is“spinal stimulation”where electrodes,usually positioned above the level of injury,provide electrical stimulation to target and disrupt pain signals before they reach the *** second approach uses“activity-dependent neuro-technologies”,in which electrodes positioned below the level of injury initiate a complex spatiotemporal pattern of stimulation at the lumbar spinal cord to generate a walking gait in the limbs(minev et al.,2015;Wagner et al.,2018).

关键词： stimulation electrodes utilizing

Study on viscosity mechanism of caffeine crystallization solutions Author links open overlay panel

在线全文

学校读者我要写书评

暂无评论

Particuology 2024年第8期91卷 341-350页

作者： Linjing Yu min Su School of Chemical Engineering Hebei University of TechnologyTianjin300130China

The solid-liquid viscous system formed by high viscosity crystallization solution impacts the flow and separation ***,it is very important to study the viscosity mechanism to improve viscosity and regulate crystallization,ensuring a seamless production ***,the viscosity of crystallization solution was taken as the measurement parameter of caffeine as a model *** investigated the viscosity mechanism of caffeine crystallization solutions by combining experiment and *** results indicated that the weak interactions between caffeine and water result in increased viscosity of the caffeine crystallization ***,caffeine crystals possess elongated needle-like shapes,featuring a substantial specific surface ***,there isπ-πstacking occurring between the(200)crystal face and(110)crystal face,effectively fostering coalescence of the crystals towards the radial side of the crystal along its elongated axis,resulting in a more viscous crystallization *** results contribute to comprehending the viscosity mechanism of crystal systems and provide theoretical foundation to enhance engineering efficiency in crystallization.

关键词： Crystallization Caffeine Viscosity mechanism Molecular simulation

Ligand-controlled NiH-catalyzed regiodivergent hydroalkylation of 2-alkenylazaarenes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2024年第6期67卷 1935-1940页

作者： Ya-Ping Shao Zhuo-min Chi Yong-min Liang State Key Laboratory of Applied Organic Chemistry School of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou730000China

A nickel-hydride(Ni H)-catalyzed migratory and nonmigratory hydroalkylation reaction of 2-alkenyl azaarenes with alkyl iodines has been established through strategic modulation of N-or P-donor *** method enables the synthesis of diverseβ-orγ-branched aromatic *** mild regiodivergent protocols exhibit wide substrate scope,excellent functional tolerance,and great reaction yield with remarkable ***,deuterium labeling studies reveal Ni H-catalyzed interrupted chain-walking mode as the mechanism behind remotedβ-selective hydroalkylation reaction—an interesting phenomenon mediated through aromatic imine directing groups.

关键词： NiH regiodivergent hydroalkylation azaarenes interrupted chain-walking

Pseudomonotonicity of Nonlinear Transformations on Euclidean Jordan Algebras

在线全文

学校读者我要写书评

暂无评论

Acta Mathematicae Applicatae Sinica 2024年第1期40卷 192-204页

作者： Yuan-min Li School of Mathematics and Statistics Xidian UniversityXi’an710071China

In this paper, we have introduced the concepts of pseudomonotonicity properties for nonlinear transformations defined on Euclidean Jordan algebras. The implications between this property and other P-properties have been studied. More importantly, we have solved the solvability problem of the nonlinear pseudomonotone complementarity problems over symmetric cones.

关键词： Euclidean Jordan algebra nonlinear transformation complementarity problem pseudomonotone

Design of efficient thermoelectric generators

在线全文

学校读者我要写书评

暂无评论

Science China Materials 2024年第1期67卷 377-378页

作者： min Liu Yanzhong Pei Interdisciplinary Materials Research Center School of Materials Science and EngineeringTongji UniversityShanghai 201804China

Thermoelectricity,which enables direct conversion between heat and electricity,undergoes impressive developments in recent decades,and is particularly promising for recovering low-grade heat of<600 K[1,2].Currently,only Bi2Te3-based thermoelectric devices are commercialized[3].However,the scarcity of tellurium(Te)(Fig.1a)is the main *** leads to numerous recent efforts on the development of efficient nonBi2Te3 thermoelectrics as potential alternatives,and successful examples(Fig.1b)include Mg3Sb2-alloys[2,3]and MgAgSb[1,2].

关键词： enable efficient electricity