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Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014

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Science China(life Sciences) 2014年第12期57卷 1149-1155页

作者： ZHAO hui ZHAO ling Zhai jiang Tao li Xiao Feng FAN Hang HONG Wen Xin zhang Yu zhu Qin YE Qing tong Yi Gang CAO Wu Chun zhang Fu Chun QIN Cheng Feng State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Guangzhou 8th People's Hospital Guangzhou Medical University

Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in *** July 2014,an unexpected large dengue outbreak has occurred in Guangdong province,China,resulting in more than 40000 patients including six *** clarify and characterize the causative agent of this outbreak,the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing(HTS).Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2(DENV-2).A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 *** complete genome of GZ05/2014 was determined and deposited in Gen Bank under the access number *** analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past *** amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/*** high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong,*** results help clarify the origin of this epidemic and predict the future status of dengue in China.

关键词： dengue virus serotype 2 virus isolation phylogenetic analysis envelope gene

Clinical and antibody characteristics reveal diverse signatures of severe and non-severe SARS-CoV-2 patients

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Infectious Diseases of Poverty 2022年第1期11卷 94-95页

作者： Hongye Wang Dongshan Yan Ya li Yanfei Gong Yulin Mai Bingxiang li Xiaoyong zhu Xinrui Wan liyun Xie HuaKe jiang min zhang ming Sun Yufeng Yao Yongzhang zhu Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical CollegeKunming650118YunnanChina 不详 School of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineOne Health CenterShanghai Jiao Tong University-The University of EdinburghShanghai200025China

Background COVID-19 pandemic continues,clarifying signatures in clinical characters and antibody responses between severe and non-severe COVID-19 cases would benefit the prognosis and *** In this study,119 serum samples from 37 severe or non-severe COVID-19 patients from the First People's Hospital of Yueyang were collected between January 25 and February *** clinical features,antibody responses targeting SARS-CoV-2 spike protein(S)and its different domains,SARS-CoV-2-specific Ig isotypes,IgG subclasses,ACE2 competitive antibodies,binding titers with FcγIIa and FcγIIb receptors,and 14 cytokines were comprehensively *** differences between severe and non-severe groups were analyzed using Mann–Whitney U test or Fisher’s exact *** Severe group including 9 patients represented lower lymphocyte count,higher neutrophil count,higher level of LDH,total bile acid(TBA)(Pminase(P=0.011),adenosine deaminase(Plity were observed in a severe group than that of a non-severe group,of which IgG1 and IgG3 were the main IgG ***-IgG were strongly correlated with S-IgG both in severe and non-severe ***,CTD-IgG was strongly correlated with S-IgG in a non-severe *** RBD-ACE2 binding inhibition was strongly associated with high titers of antibody(S-IgG1,S-IgG3,NTD-IgG,RBD-IgA,NTD-IgA,and CTD-IgA)especially RBD-IgG and CTD-IgG in the severe group,while in the non-severe group,S-IgG3,RBD-IgG,NTD-IgG,and NTD-IgM were correlated with ACE2 blocking rate.S-IgG1,NTD-IgM and S-IgM were negatively associated with illness day in a severe group,while S-IgG3,RBD-IgA,CTD-IgA in the severe group(r=0.363,P=0.011)and S-IgG1,NTD-IgA,CTD-IgA in the non-severe group were positive

关键词： SARS-CoV-2 COVID-19 Severe patient Cytokine Immune response

CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells

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Signal Transduction and Targeted Therapy 2021年第1期6卷 95-104页

作者： Ke Wang Wei Chen Zheng zhang Yongqiang Deng Jian-Qi lian Peng Du Ding Wei Yang zhang Xiu-Xuan Sun li Gong Xu Yang Lei He Lei zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai zhang Bin Wang Yu-Meng zhu Gang Nan Jian-li jiang ling li Jiao Wu Peng lin Wan Huang liangzhi Xie Zhao-hui Zheng Kui zhang Jin-lin Miao Hong-Yong Cui min Huang Jun zhang ling Fu Xiang-min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu ying-ying liu Qing-Yi Wang huijie Bian Ping zhuZhi-Nan Chen National Translational Science Center for Molecular Medicine&Department of Cell Biology Fourth Military Medical UniversityXi’an 710032China Beijing Institute of Biotechnology Beijing 100071China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China Tangdu Hospital Fourth Military Medical UniversityXi’an 710038China MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter Xi’an Jiaotong UniversityXi’an 710049China Sino Biological Inc. Beijing 100176China Department of Clinical Immunology Xijing HospitalFourth Military Medical UniversityXi’an 710032China School of Basic Medicine Fourth Military Medical UniversityXi’an 710032China

In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until ***-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike *** ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the *** the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its ***,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike *** loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 *** of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular *** loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type ***,virions are observed in lymphocytes of lung tissue from a COVID-19 *** T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by ***,CD147 mediates virus entering host cells by ***,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19.

关键词： ACE2 CD147 intestine

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运动病动物模型研究新进展

实验动物科学 2024年第1期41卷 94-98页

作者：张卓儒张敏李聪聪刘玉辉姜一弘畅通朱靖黄立桂周小燕王小成延安大学医学院病理与病理生理学科组延安716000 中国人民解放军空军军医大学航空航天医学系航空航天临床医学中心西安710032 中国人民解放军空军军医大学第一附属医院空勤科西安710032 中国人民解放军联勤保障部队第908医院耳鼻咽喉头颈外科南昌360104

随着运动病研究的不断深入,运动病模型的动物种类不断增多,判断标准和指标逐渐采用客观的行为学指标和生理学指标结合的方法。本文就近年来常用的运动病动物模型,从动物种类、造模方法和模型标准与判断指标,分析总结其方法和特点,整理... 详细信息

随着运动病研究的不断深入,运动病模型的动物种类不断增多,判断标准和指标逐渐采用客观的行为学指标和生理学指标结合的方法。本文就近年来常用的运动病动物模型,从动物种类、造模方法和模型标准与判断指标,分析总结其方法和特点,整理收集运动病动物模型的评价方法,为运动病发病机制的探索、疾病严重程度客观评价、抗运动病药物研发及前庭稳定性训练方案制定等研究提供可靠的实验动物建模方法和观察指标。

关键词：运动病动物模型建模方法评价方法

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木结构拱形金属屋面防水施工技术研究

建筑技术开发 2024年第8期51卷 39-41页

作者：张慧敏高学龙江庆宝朱云雷袁洵中建八局装饰工程有限公司上海200122

以上海市交通大学闵行校区的木结构建筑为研究对象,研究木结构拱形金属屋面防水技术。首先,选择合理的材料与施工工艺,遵循以排为主、排防结合的原则进行防水设计和方案实施。旨在为后续的工程项目提供一定的参考,推进木结构相关领域的... 详细信息

以上海市交通大学闵行校区的木结构建筑为研究对象,研究木结构拱形金属屋面防水技术。首先,选择合理的材料与施工工艺,遵循以排为主、排防结合的原则进行防水设计和方案实施。旨在为后续的工程项目提供一定的参考,推进木结构相关领域的发展。

关键词：木结构金属屋面防水

一项基于全国多中心前瞻性登记队列研究(PACAGE)的中国胃癌和结直肠癌患者术后并发症现状分析

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中华胃肠外科杂志 2024年第3期27卷 247-260页

作者：张书勤吴舟桥霍博文徐惠宁赵康靖昌庆刘凤林余江李正荣张健臧潞蒿汉坤郑朝辉李勇樊林黄华梁品吴斌朱甲明牛兆建朱玲华宋武尤俊燕速李子禹青海大学医学部公共卫生系青海大学附属医院西宁810001 北京大学肿瘤医院胃肠肿瘤中心北京100142 青海大学附属医院胃肠肿瘤外科西宁810001 山东第一医科大学附属省立医院胃肠外科济南250021 复旦大学附属肿瘤医院胃外科上海200025 南方医科大学附属南方医院普通外科广州510515 南昌大学附属第一医院胃肠外科南昌330006 浙江大学附属第一医院胃肠外科杭州310003 上海交通大学医学院附属瑞金医院胃肠外科上海200025 复旦大学附属华山医院胃肠外科上海200040 福建医科大学附属协和医院胃外科福州350001 广东省人民医院胃肠外科广州510080 西安交通大学附属第一医院普通外科西安710061 大连医科大学附属第一医院胃肠外科大连116011 中国协和医科大学附属协和医院基本外科北京100032 中国医科大学第一附属医院胃肠肿瘤外科沈阳110002 青岛大学附属医院胃肠外科青岛266000 浙江大学附属邵逸夫医院胃肠外科杭州310009 中山大学第一附属医院胃肠外科广州510062 厦门大学第一附属医院胃肠肿瘤外科厦门361003 徐州医科大学附属宿迁医院感染管理科

目的探讨我国胃癌和结直肠癌患者术后并发症的发生率,并评估其危险因素。方法本研究采用全国多中心前瞻性数据登记队列研究方法,数据来自中国胃肠肿瘤外科联盟发起的胃肠外科手术后腹部并发症现状研究数据库(PACAGE)。PACAGE数据库自201... 详细信息

目的探讨我国胃癌和结直肠癌患者术后并发症的发生率,并评估其危险因素。方法本研究采用全国多中心前瞻性数据登记队列研究方法,数据来自中国胃肠肿瘤外科联盟发起的胃肠外科手术后腹部并发症现状研究数据库(PACAGE)。PACAGE数据库自2018年12月至2020年12月期间,前瞻性收集了20家医学中心胃癌和结直肠癌患者一般人口统计学数据、围手术期治疗及术后并发症相关数据,根据是否发生术后并发症进行分组,参照胃肠肿瘤外科术后并发症相关专家共识和Clavien-Dindo分级标准,对并发症进行分类及分级。通过绘制热图,将不同分级的术后并发症发生率可视化;采用多因素非条件logistic回归模型,分析发生术后并发症的独立危险因素。结果共纳入3926例胃癌和结直肠癌患者,657例患者共发生876例次术后并发症,总体并发症发生率16.7%(657/3926),Ⅲ级及以上严重并发症发生率4.0%(156/3926),Ⅴ级并发症发生率0.2%(7/3926)。胃癌患者2271例,术后并发症发生率18.1%(412/2271),严重并发症发生率4.7%(106/2271);结直肠癌患者1655例,术后并发症发生率14.8%(245/1655),严重并发症发生率3.0%(50/1655)。胃癌和结直肠癌患者吻合口漏的发生率分别是3.3%(74/2271)和3.4%(56/1655)。腹腔感染在各类并发症中占比最高,在胃癌和结直肠癌患者中分别为28.7%(164/572)和39.5%(120/304)。在所有术后并发症严重程度分级中,Ⅱ级并发症占比最高,胃癌和结直肠癌分别占65.4%(374/572)和56.6%(172/304)。多因素分析显示:(1)胃癌组患者发生术后并发症的独立影响因素包括:有术前合并症(OR=2.54,95%CI:1.51~4.28,P180 min(OR=1.81,95%CI:1.42~2.31,P50 ml(OR=1.29,95%CI:1.01~1.63,P=0.038)和相比于全胃切除术的远端胃切除(OR=0.65,95%CI:0.51~0.83,P<0.001);(2)肠癌组患者发生术后并发症的独立影响因素包括:女性(OR=0.60,95%CI:0.44~0.80,P<0.001)、有术前合并症(OR=2.73,95%CI:1.25~5.99,P=0.030)、接受新辅助治疗(OR=1.83,95%CI:1.23~2.72,P=0.008)、腹腔镜手术(OR=0.47,95%CI:0.30~0.72,P=0.022)和相比于低位前切术的腹会阴联合切术(OR=2.74,95%CI:1.71~4.41,P<0.001)。结论胃癌和结直肠癌患者术后各类感染相关的并发症最为常见,虽然两者术后发生并发症的危险因素各有不同,但有术前合并症、行新辅助治疗以及手术切除范围,是胃癌和结直肠癌患者术后发生并发症的共同影响因素。

关键词：胃肿瘤结直肠肿瘤术后并发症风险因素

Ischemic or toxic injury: A challenging diagnosis and treatment of drug-induced stenosis of the sigmoid colon

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World Journal of Gastroenterology 2017年第21期23卷 3934-3944页

作者： Zong-ming zhang Xiang-Chun lin li Ma An-Qin Jin Fang-Cai lin zhuo liu li-min liu Chong zhang Na zhang li-Juan Huo Xue-liang jiang Feng Kang Hong-Jun Qin Qiu-Yang li Hong-Wei Yu Hai Deng ming-Wen zhu Zi-Xu liu Bai-jiang Wan Hai-Yan Yang Jia-Hong liao Xu Luo You-Wei li Wen-Ping Wei Meng-Meng Song Yue Zhao Xue-ying Shi Zhao-hui Lu Department of General Surgery Beijing Electric Power HospitalState Grid Corporation of ChinaCapital Medical University Department of Gastroenterology Peking University International Hospital Asian liver Center and Department of Surgery School of MedicineStanford University Department of Gastroenterology Beijing Electric Power HospitalState Grid Corporation of ChinaCapital Medical University Department of Gastroenterology First HospitalShanxi Medical University Department of Gastroenterology Jinan Military General Hospital Department of Radiology Beijing Electric Power HospitalState Grid Corporation of ChinaCapital Medical University Department of Pathology Beijing Electric Power HospitalState Grid Corporation of ChinaCapital Medical University Department of Pathology Peking University Third HospitalSchool of Basic Medical SciencesPeking University Health Science Center1 Department of Pathology Peking Union Medical College HospitalChinese Academy of Medical Sciences

A 48-year-old woman was admitted with 15-mo history of abdominal pain, diarrhea and hematochezia, and 5-mo history of defecation difficulty. She had been successively admitted to nine hospitals, with an initial diagnosis of inflammatory bowel disease with stenotic sigmoid colon. Findings from computed tomography virtual colonoscopy, radiography with meglumine diatrizoate, endoscopic balloon dilatation, metallic stent implantation and later overall colonoscopy, coupled with the newfound knowledge of compound Qingdai pill-taking, led to a subsequent diagnosis of ischemic or toxic bowel disease with sigmoid colon stenosis. The patient was successfully treated by laparoscopic sigmoid colectomy, and postoperative pathological examination revealed ischemic or toxic injury of the sigmoid colon, providing a final diagnosis of druginduced sigmoid colon stenosis. This case highlights that adequate awareness of drug-induced colon stenosis has a decisive role in avoiding misdiagnosis and mistreatment. The diagnostic and therapeutic experiences learnt from this case suggest that endoscopic balloon expansion and colonic metallic stent implantation as bridge treatments were demonstrated as crucial for the differential diagnosis of benign colonic stenosis. Skillful surgical technique and appropriate perioperative management helped to ensure the safety of our patient in subsequent surgery after long-term use of glucocorticoids.

关键词： Benign colonic stenosis Compound Qingdai pill Self-expandable memory metallic stent

Analysis of localization effect in blue-violet light emitting InGaN/GaN multiple quantum wells with different well widths

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Chinese Physics B 2017年第1期26卷 498-502页

作者： Xiang li De-Gang Zhao De-Sheng jiang Jing Yang Ping Chen Zong-Shun liu Jian-Jun zhu Wei liu Xiao-Guang He Xiao-Jing li Feng liang Jian-Ping liu li-Qun zhang hui Yang Yuan-Tao zhang Guo-tong Du Heng Long Mo li State Key Laboratory on Integrated Optoelectronics Institute of Semiconductors Chinese Academy of Sciences Beijing 100083 China University of Chinese Academy of Sciences Beijing 100049 China Suzhou Institute of Nano-tech and Nano-bionics Chinese Academy of Sciences Suzhou 215125 China State Key Laboratory on Integrated Optoelectronics College of Electronic Science and Engineering Jilin University Changchun 130023 China Microsystem & Terahertz Research Center Chinese Academy of Engineering Physics Chengdu 610200 China

Four blue-violet light emitting InGaN/GaN multiple quantum well（MQW） structures with different well widths are grown by metal–organic chemical vapor deposition. The carrier localization effect in these samples is investigated mainly by temperature-dependent photoluminescence measurements. It is found that the localization effect is enhanced as the well width increases from 1.8 nm to 3.6 nm in our experiments. The temperature induced PL peak blueshift and linewidth variation increase with increasing well width, implying that a greater amplitude of potential fluctuation as well as more localization states exist in wider wells. In addition, it is noted that the broadening of the PL spectra always occurs mainly on the low-energy side of the PL spectra due to the temperature-induced band-gap shrinkage, while in the case of the widest well, a large extension of the spectral curve also occurs in the high energy sides due to the existence of more shallow localized centers.

关键词： InGaN/GaN multiple quantum wells localization effect well thickness

非手术疗法治疗腰椎间盘突出症的循证实践指南

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中国循证医学杂志 2024年第2期24卷 125-148页

作者：葛龙李镜尚文茹邢新牛军强闵凯莉惠旭何升华姜宏焦锋姚嫄董敬石徐倩郝国雄冷向阳李刚林定坤刘军马维虎莫文史晓林宋敏王拥军王勇平姚啸生于杰詹红生朱立国谢兴文邢丹杜亮卢卫忠郭珂李紫珺李秀霞李美萱李艳飞张立存赵继荣杨克虎兰州大学公共卫生学院卫生政策与管理学系兰州730000 世界卫生组织指南实施与知识转化合作中心/甘肃省医学指南行业技术中心兰州730000 兰州大学基础医学院循证医学中心兰州730000 兰州大学第一医院兰州730000 兰州大学第二临床医学院兰州730000 深圳市中医院广东深圳518033 南京中医药大学苏州附属医院江苏苏州215000 广州市中西医结合医院广州510000 兰州大学第一临床医学院兰州730000 甘肃中医药大学兰州730000 长春中医药大学长春130000 山东中医药大学附属医院济南250000 广州中医药大学广州510000 广东省第二中医院广州510000 宁波市第六医院浙江宁波315000 上海中医药大学附属龙华医院上海200000 浙江中医药大学附属第二医院杭州310000 上海中医药大学上海200000 辽宁中医药大学附属医院沈阳110000 中国中医科学院望京医院北京100000 上海中医药大学附属曙光医院上海200000 甘肃中医药大学附属医院兰州730000 北京大学人民医院北京100000 四川大学华西医院成都610041 重庆市中医院重庆404100

腰椎间盘突出症(LDH)是导致腰腿痛最常见原因之一,严重影响患者的生活质量。非手术疗法作为LDH的一线治疗方案,可缓解80%~90%LDH患者的临床症状。本指南遵循《中国制订/修订临床诊疗指南的指导原则(2022版)》和《世界卫生组织指南制定手... 详细信息

腰椎间盘突出症(LDH)是导致腰腿痛最常见原因之一,严重影响患者的生活质量。非手术疗法作为LDH的一线治疗方案,可缓解80%~90%LDH患者的临床症状。本指南遵循《中国制订/修订临床诊疗指南的指导原则(2022版)》和《世界卫生组织指南制定手册(2014版)》,成立指南工作组。结合文献回顾和专家调研,确定14个临床问题。通过系统的证据检索、评价与分级,形成推荐意见决策表;采用德尔菲法对初步形成的推荐意见进行专家共识,最终形成19条非手术疗法治疗LDH的推荐意见。本指南可供中西医骨科相关从业人员参考和使用。

关键词：腰椎间盘突出症非手术疗法循证实践指南