检索结果-南通市图书馆

Neural-like cells from adipose-derived stem cells for cavernous nerve injury in rats

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2019年第6期14卷 1085-1090页

作者： Cheng-Cheng Ying Mei Yang Yong Wang Yong-Lian Guo Wan-Li Hu Xin-min zheng Department of Urology the Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and Technology Department of Endocrinology Tongren Hospital of Wuhan University (Wuhan Third Hospital) Department of Urology Zhongnan Hospital of Wuhan University

Although the remaining nerve tissue can regenerate and partly restore erectile function when the cavernous nerve is compressed/severed and function lost, the limited regenerative ability of these nerve tissues often fails to meet clinical needs. Adipose-derived stem cells are easy to obtain and culture, and can differentiate into neural cells. Their proliferation rate is easy to control and they may be used to help restore injured cavernous nerve function. Sprague-Dawley male rats(n = 45) were equally randomized into three groups: fifteen rats as a sham-operated group, fifteen rats as a bilateral nerve crush(BINC) group(with no further intervention), fifteen rats as a BINC with intracavernous injection of one million neural-like cells from adipose-derived stem cells(NAS)(BINC + NAS) group. After 4 weeks, erectile function was assessed by stimulating the cavernous body. The number of myelinated axons in the dorsal cavernous nerve was determined by toluidine blue staining. The area of neuronal nitric oxide synthase-positive fibers in the dorsal penile nerve was measured by immunohistochemical staining. Masson staining was used to analyze the ratio of smooth muscle to collagen in penile tissue. The results demonstrate that maximal intracavernous pressure, the ratio of maximal intracavernous pressure to mean arterial pressure, the numbers of myelinated axons and neuronal nitric oxide synthase-positive fibers in the dorsal penile nerve, and the ratio of smooth muscle to collagen could be increased after cell transplantation. These findings indicate that neural-like cells from adipose-derived stem cells can effectively alleviate cavernous nerve injury and improve erectile function. All animal experiments were approved by the Animal Ethics Committee of Huazhong University of Science and Technology, China(approval No. 2017-1925) on September 15, 2017.

关键词： nerve regeneration adipose-derived neural stem cells corpus cavernosum cavernous nerve erectile dysfunction radical prostatectomy neurons cell differentiation neural regeneration

Dosimetry Comparison between Volumetric Modulated Arc Therapy with RapidArc and Fixed Field Dynamic IMRT for Local-Regionally Advanced Nasopharyngeal Carcinoma

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2011年第4期23卷 259-264页

作者： Bao-min zheng Xiao-xia Dong Hao Wu You-jia Duan Shu-kui Han Yan Sun Key Laboratory of Carcinogenesis and Translational Research(ministry of Education) Department of RadiotherapyPeking University School of Oncology Beijing Cancer Hospital & Institute Beijing 100142 China

Objective: A dosimetric study was performed to evaluate the performance of volumetric modulated arc radiotherapy with RapidArc on locally advanced nasopharyngeal carcinoma (NPC). Methods: The CT scan data sets of 20 patients of locally advanced NPC were selected randomly. The plans were managed using volumetric modulated arc with RapidArc and fixed nine-field coplanar dynamic intensity-modulated radiotherapy (IMRT) for these patients. The dosimetry of the planning target volumes (PTV), the organs at risk (OARs) and the healthy tissue were evaluated. The dose prescription was set to 70 Gy to the primary tumor and 60 Gy to the clinical target volumes (CTV) in 33 fractions. Each fraction applied daily, five fractions per week. The monitor unit (MU) values and the delivery time were scored to evaluate the expected treatment efficiency. Results: Both techniques had reached clinical treatment’s requirement. The mean dose (Dmean), maximum dose (Dmax) and minimum dose (Dmin) in RapidArc and fixed field IMRT for PTV were 68.4±0.6 Gy, 74.8±0.9 Gy and 56.8±1.1 Gy; and 67.6±0.6 Gy, 73.8±0.4 Gy and 57.5±0.6 Gy (P0.05). Compared to IMRT, RapidArc allowed a reduction of Dmean to the brain stem, mandible and optic nerves of 14.1% (P<0.05), 5.6% (P<0.05) and 12.2% (P<0.05), respectively. For the healthy tissue and the whole absorbed dose, Dmean of RapidArc was reduced by 3.6% (P<0.05), and 3.7% (P<0.05), respectively. The Dmean to the parotids, the spinal cord and the lens had no statistical difference among them. The mean MU values of RapidArc and IMRT were 550 and 1,379. The mean treatment time of RapidArc and IMRT was 165 s and 447 s. Compared to IMRT, the delivery time and the MU values of RapidArc were reduced by 63% and 60%, respectively. Conclusion: For locally advanced NPC, both RapidArc and IMRT reached the c

关键词： Volumetric modulated arc therapy Intensity-modulated radiotherapy Dosimetry Target volume Nasopharyngeal carcinoma

Active sub-reflector research for a large radio telescope

在线全文

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2020年第1期20卷 69-76页

作者： Jin-Qing Wang Rong-Bin Zhao Yong-Chen Jiang zheng-Xiong Sun Lin-Feng Yu Li Fu Qin-Hui Liu Wei-min zheng Zhi-Qiang Shen Shanghai Astronomical Observatory Chinese Academy of SciencesShanghai 200030China Key Laboratory of Radio Astronomy Chinese Academy of SciencesNanjing 210008China Shanghai Key Laboratory of Space Navigation and Positioning Techniques Shanghai 430074China

This paper proposes an active sub-reflector suitable for large radio telescopes,which can compensate both of the deformation of the main reflector and sub-reflector position *** mathematical formula of the main reflector deformation compensated by the sub-reflector is deduced based on Cassegrain and Gregory antenna *** position of the sub-reflector is adjustable to compensate for defocusing errors on high and low elevations,which are mainly caused by the deformation of the sub-reflector supporting *** this paper,the method of obtaining the optimum position of the sub-reflector from the aperture phase by the interferometric method is *** actual measurement is verified on the Tianma 65 m radio telescope,which provides a new way to diagnose the position error of the sub-reflector.

关键词： radio telescope deformable sub-reflector model holography phase

Argentum 5-Hydroxy-7-methoxy-2-phenyl-4H-chromen-4-one-6-sulfonate:Synthesis,Crystal Structure and Antitumor Activity

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2020年第10期39卷 1898-1905,1746页

作者： LI Wu-Wu zheng min-Yan GAO Yi-Hong ZHANG Zun-Ting College of Chemistry&Chemical Engineering Xianyang Normal UniversityXianyang 712000China Key Laboratory of the ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China and School of Chemistry and Chemical EngineeringShaanxi Normal UniversityXi'an 710062China

In order to improve the water solubility and bioavailability of 5-hydroxy-7-methoxyflavone,argentum 5-hydroxy-7-methoxy-2-phenyl-4H-chromen-4-one-6-sulfonate[Ag4(H2O)6](C(16)H(11)O4SO3)4·H2O(1,C(16)H(11)O4SO3=5-hydroxy-7-methoxy-2-phenyl-4H-chromen-4-one-6-sulfonate)was synthesized by sulfonation *** structure of 1 was characterized by FT-IR,elemental analysis and X-ray single-crystal *** 1 belongs to the triclinic system,space group P1,a=8.077(4),b=12.365(4),c=17.735(7)A,V=1685.0(12)A3,Z=1,μ=1.372 mm^–1,Dc=1.936 g/cm^3,F(000)=984,the final R=0.0819 and wR=0.2332 with I>2σ(I).3D structure of 1 exhibits alternating organic and inorganic regions.O–H×××O hydrogen bonds and Ag–O coordination interactions exist among crystal water,coordinated water and sulfo group,which constructed an organic *** skeletons form organic region of *** group is the bridge linking these two *** in vitro antitumor activity of 1 against human lymphoma cells U937 and human breast cancer cells MCF-7 were evaluated with CCK-8 *** result shows that 1 showed inhibitory activity against tumour cell U937 and MCF-7,and indicated that flavone sulfonate derivatives may be potential leads for further biological screenings and may generate drug-like molecules.

关键词： argentum 5-hydroxy-7-methoxy-2-phenyl-4H-chromen-4-one-6-sulfonate sulfonation reaction crystal structure antitumor activity

Modal identification based on Hilbert-Huang Transform of structural response with S VD preprocessing

在线全文

学校读者我要写书评

暂无评论

Acta Mechanica Sinica 2009年第6期25卷 883-888页

作者： min zheng Fan Shen Yuping Dou Xiaoyan Yan College of Civil Aviation,Nanjing University of Aeronautics and Astronautics,210016 Nanjing. China College of Civil Aviation Nanjing University of Aeronautics and Astronautics 210016 Nanjing China

In recent years, Empirical mode decomposition and Hilbert spectral analysis have been combined to identify system parameters. Singular-Value Decomposition is pro- posed as a signal preprocessing technique of Hilbert-Huang Transform to extract modal parameters for closely spaced modes and low-energy components. The proposed method is applied to a simulated airplane model built in Automatic Dynamic Analysis of Mechanical Systems software. The results demonstrate that the identified modal parameters are in good agreement with the baseline model.

关键词： Modal identification . Hilbert-Huang Transforms - Singular-value decomposition . Signal processing

Influence of catalyst support structure on ethene/decene metathesis and coke formation over WO_3/SiO_2 catalyst

在线全文

学校读者我要写书评

暂无评论

Petroleum Science 2013年第1期10卷 112-119页

作者： zheng min Chen Sheng-Li Zhang Jun-Hui Liu Yan Sang Lei You Ju Wang Xiao-Dong State Key Laboratory of Heavy Oil Processing China University of Petroleum

8wt%WO3/SiO2 metathesis （disproportionation） catalysts with different pore structures were prepared by the incipient-wetness-impregnation method. The as-synthesized catalysts were characterized by N2 adsorpfion-desorption, scanning electron microscopy （SEM）, X-ray diffraction （XRD）, UV-visible diffuse reflectance spectroscopy （DRS） and scanning transmission electron microscopy-high-angle annular dark field （STEM HAADF）. The results of STEM HAADF showed that WO3 species were not uniformly distributed on the SiO2 support. The experimental results of 8wt%WO3/SiO2 performance in ethene/decene metathesis revealed that the catalytic effect of 8wt%WO3/SiO2 catalyst and coke formation over it were closely related to the support pore structure： The 8wt%WO3/SiO2 catalyst with a more complicated pore structure showed better catalytic performance but the coke deposition rate was also faster.

关键词： Metathesis WO3/SiO2 catalysts pore structure coke formation

Oxidized low-density lipoprotein stimulates CD206 positive macrophages upregulating CD44 and CD133 expression in colorectal cancer with high-fat diet

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2022年第34期28卷 4993-5006页

作者： Shi-min zheng Hao Chen Wei-Hong Sha Xiao-Fen Chen Jian-Bin Yin Xiao-Bo Zhu Zhong-Wen zheng Juan Ma Department of Gastroenterology and Hepatology Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080Guangdong ProvinceChina Medical College Shantou UniversityShantou 515041Guangdong ProvinceChina Medical College Southern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Center for Orthopaedic Surgery The Third Affiliated Hospital of Southern Medical UniversityGuangzhou 510630Guangdong ProvinceChina

BACKGROUND Oxidized low-density lipoprotein(ox-LDL),which is abnormally increased in the serum of colorectal cancer(CRC)patients consuming a high-fat diet(HFD),may be one of the risk factors for the development of ***-LDL exerts a regulatory effect on macrophages and may influence CRC through the tumor *** role of ox-LDL in CRC remains *** To investigate the role of ox-LDL through macrophages in HFD associated *** The expression of ox-LDL and CD206 was detected in colorectal tissues of CRC patients with hyperlipidemia and HFD-fed mice by *** stimulated the macrophages with 20μg/mL ox-LDL and assessed the expression levels of CD206 and the cytokines by cell fluorescence and quantitative polymerase chain *** further knocked down LOX-1,the surface receptor of ox-LDL,to confirm the function of ox-LDL in ***,LoVo cells were co-cultured with the stimulated macrophages to analyze the CD44 and CD133 expression by western *** The expression of ox-LDL and the CD206 was significantly increased in the stroma of colorectal tissues of CRC patients with hyperlipidemia,and also upregulated in the HFD-fed ***,an increased level of CD206 and decreased level of inducible nitric oxide synthase were observed in macrophages after ox-LDL continuous *** effects were inhibited when the surface receptor LOX-1 was knocked down in ***-LDL could induce CD206+macrophages,which resulted in high expression of CD44 and CD133 in co-cultured LoVo *** Ox-LDL stimulates CD206+macrophages to upregulate CD44 and CD133 expression in HFD related CRC.

关键词： Oxidized low-density lipoprotein CD206 positive macrophages CD44 CD133

One new genus and three new species of wingless grasshoppers from Xizang,China(Orthoptera:Acridoidea)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

动物分类学报 2014年第2期39卷 263-268页

作者： Zhe-min zheng Li-Liang Lin Hong-Li Zhang Hui-Hua Zeng Institute of Zoology Shaanxi Normal University

One new genus and three new species of wingless grasshoppers, i.e, Pseudozubovskia gen. nov., Pseudozubovskia xizangensis sp. nov., Eokingdonella gongbugyanda Sla. nov., Dysanema magna sp. nov. are described. The type... 详细信息

关键词： Orthoptera Acridoidea wingless grasshopper new genus new species.

Effects of primary microglia and astrocytes on neural stem cells in in vitro and in vivo models of ischemic stroke

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第9期16卷 1677-1685页

作者： Sheng-Jun Wen Xi-min zheng Li-Fen Liu Na-Na Li Hai-An Mao Liang Huang Qiong-Lan Yuan Department of Neurology Shanghai Tongji HospitalTongji University School of MedicineShanghaiChina

Transplantation of neural stem cells(NSCs) can protect neurons in animal stroke models;however, their low rates of survival and neuronal differentiation limit their clinical application. Glial niches, an important location of neural stem cells, regulate survival, proliferation and differentiation of neural stem cells. However, the effects of activated glial cells on neural stem cells remain unclear. In the present study, we explored the effects of activated astrocytes and microglia on neural stem cells in vitro stroke models. We also investigated the effects of combined transplantation of neural stem cells and glial cells after stroke in rats. In a Transwell co-culture system, primary cultured astrocytes, microglia or mixed glial cells were exposed to glutamate or H_2O_2 and then seeded in the upper inserts, while primary neural stem cells were seeded in the lower uncoated wells and cultured for 7 days. Our results showed that microglia were conducive to neurosphere formation and had no effects on apoptosis within neurospheres, while astrocytes and mixed glial cells were conducive to neurosphere differentiation and reduced apoptosis within neurospheres, regardless of their pretreatment. In contrast, microglia and astrocytes induced neuronal differentiation of neural stem cells in differentiation medium, regardless of their pretreatment, with an exception of astrocytes pretreated with H_2O_2. Rat models of ischemic stroke were established by occlusion of the middle cerebral artery. Three days later, 5 × 10~5 neural stem cells with microglia or astrocytes were injected into the right lateral ventricle. Neural stem cell/astrocyte-treated rats displayed better improvement of neurological deficits than neural stem cell only-treated rats at 4 days after cell transplantation. Moreover, neural stem cell/microglia-, and neural stem cell/astrocyte-treated rats showed a significant decrease in ischemic volume compared with neural stem celltreated rats. These findings indicate

关键词： astrocytes glutamate microglia neural stem cells neurogenesis neurons peroxide repair stroke