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论习近平文化思想的情感意蕴

理论导刊 2024年第3期 4-11页

作者：刁敏靳玉军西南大学马克思主义学院重庆400715 西南大学马克思主义理论研究中心重庆400715

习近平文化思想是“情”“理”交融的理论体系,既有严密的逻辑思维,也有深厚的情感意蕴。在其形成过程中,中国共产党领导文化建设的情感面相、人民对文化的情感诉求以及社会文化发展的自信基调等情感因素发挥着重要作用,确立了这一思想... 详细信息

习近平文化思想是“情”“理”交融的理论体系,既有严密的逻辑思维,也有深厚的情感意蕴。在其形成过程中,中国共产党领导文化建设的情感面相、人民对文化的情感诉求以及社会文化发展的自信基调等情感因素发挥着重要作用,确立了这一思想的共情力量、人民品格与强国使命。在理论层面,习近平文化思想蕴含对马克思主义的信仰之情、对中华文化的自信之情以及对世界文明的尊重之情等丰富的情感表达,体现出生动性与真实性。在文化工作实践层面,习近平文化思想对文艺创作、思想道德建设、意识形态工作、中华文明国际传播等具体领域也提出明确的情感要求,强调发挥情感的特殊机制与优势。深入剖析习近平文化思想的情感意蕴,既能扩展对习近平文化思想的认识视域,更好地加以宣介和落实,也能为在新的起点上继续加强社会主义文化建设提供情感密钥指导。

关键词：习近平文化思想明体达用情感文化建设

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Arabidopsis AIP1-1 regulates the organization of apical actin filaments by promoting their turnover in pollen tubes

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Science China(Life Sciences) 2020年第2期63卷 239-250页

作者： min diao Xin Li Shanjin Huang Institute of Botany Chinese Academy of SciencesBeijing 100093China Center for Plant Biology School of Life SciencesTsinghua UniversityBeijing 100084China University of Chinese Academy of Sciences Beijing 100049China iHuman Institute Shanghai Tech UniversityShanghai 201210China

Apical actin filaments are highly dynamic structures that are crucial for rapid pollen tube growth, but the mechanisms regulating their dynamics and spatial organization remain incompletely understood. We here identify that AtAIP1-1 is important for regulating the turnover and organization of apical actin filaments in pollen tubes. AtAIP1-1 is distributed uniformly in the pollen tube and loss of function of AtAIP1-1 affects the organization of the actin cytoskeleton in the pollen tube. Specifically, actin filaments became disorganized within the apical region of aip1-1 pollen tubes. Consistent with the role of apical actin filaments in spatially restricting vesicles in pollen tubes, the apical region occupied by vesicles becomes enlarged in aip1-1 pollen tubes compared to WT. Using ADF1 as a representative actin-depolymerizing factor, we demonstrate that AtAIP1-1 enhances ADF1-mediated actin depolymerization and filament severing in vitro, although AtAIP1-1 alone does not have an obvious effect on actin assembly and disassembly. The dynamics of apical actin filaments are reduced in aip1-1 pollen tubes compared to WT. Our study suggests that AtAIP1-1 works together with ADF to act as a module in regulating the dynamics of apical actin filaments to facilitate the construction of the unique "apical actin structure" in the pollen tube.

关键词： pollen tube apical actin structure actin dynamics actin turnover ADF AIP1

Calcium imaging in Arabidopsis pollen cells using G-CaMP5

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Journal of Integrative Plant Biology 2018年第9期60卷 897-906页

作者： min diao Xiaolu Qu Shanjin Huang Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Center for Plant Biology School of Life SciencesTsinghua UniversityBeijing 100084China University of Chinese Academy of Sciences Beijing 100049China

Calcium （Ca2 ＋） signaling has been implicated in poJ]en germination and pollen tube growth. To date, however, we still know very little about how exactly Ca2＋ signaling links to various physiological subcellular processes during pollen germination and pollen tube growth. Given that Ca2＋ signaling is tightly related to the cytosolic concentration and dynamics of Ca2＋, it is vital to trace the dynamic changes in Ca＋ levels in order to decode Ca2＋ signaling. Here, we demonstrate that G-CaMP5 serves well as an indicator for monitoring cytosolic Ca2＋ dynamics in pollen cells. Using this probe, we show that cytosolic Ca2＋ changes dramatically during pollen germination, and, as reported previously, Ca2＋ forms a tip-focused gradient in the pollen tube and undergoes oscillation in the tip region during pollen tube growth. In particular, using G-CaMP5 allowed us to capture the dynamic changes in the cytosolic Ca2＋ concentration （[Ca2＋ ]cyt） in pollen tubes in response to various exogenous treatments. Our data suggest that G-CaMP5 is a suitable probe for monitoring the dynamics of [Ca2＋ ]cyt in pollen cells.

关键词： Calcium imaging in Arabidopsis pollen cells using G-CaMP5 MP

Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning

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Molecular Plant 2017年第7期10卷 930-947页

作者： Xiaolu Qu Ruihui Zhang Meng Zhang min diao Yongbiao Xue Shanjin Huang Center for Plant Biology School of Life Sciences Tsinghua University Beijing 100084 China Key Laboratory of Plant Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100101 China

Polarized tip growth is a fundamental cellular process in many eukaryotes. In this study, we examined the dynamic restructuring of the actin cytoskeleton and its relationship to vesicle transport during pollen tip growth in Arabidopsis. We found that actin filaments originating from the apical membrane form a specialized structure consisting of longitudinally aligned actin bundles at the cortex and inner cytoplasmic fila- ments with a distinct distribution. Using actin-based pharmacological treatments and genetic mutants in combination with FRAP （fluorescence recovery after photobleaching） technology to visualize the transport of vesicles within the growth domain of pollen tubes, we demonstrated that cortical actin filaments facilitate tip-ward vesicle transport. We also discovered that the inner apical actin filaments prevent backward movement of vesicles, thus ensuring that sufficient vesicles accumulate at the pollen tube tip to support the rapid growth of the pollen tube. The combinatorial effect of cortical and internal apical actin filaments perfectly explains the generation of the inverted ＂V＂ cone-shaped vesicle distribution pattern at the pollen tube tip. When pollen tubes turn, apical actin filaments at the facing side undergo depolymerization and repolymerization to reorient the apical actin structure toward the new growth direction. This actin restructuring precedes vesicle accumulation and changes in tube morphology. Thus, our study provides new insights into the functional relationship between actin dynamics and vesicle transport during rapid and directional pollen tube growth.

关键词： pollen tube clear zone apical actin structure actin dynamics vesicle trafficking myosin

Emulsifying property,antioxidant activity,and bitterness of soybean protein isolate hydrolysate obtained by Corolase PP under high hydrostatic pressure

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Food Science and Human Wellness 2024年第3期13卷 1271-1278页

作者： Haining Guan Chunmei Feng min Ren Xiaojun Xu Dengyong Liu Xiaoqin diao College of Food Science and Technology Bohai UniversityJinzhou 121013China

Enzymatic hydrolysis of proteins can enhance their emulsifying properties and antioxidant ***,the problem related to the hydrolysis of proteins was the generation of the bitter ***,high hydrostatic pressure(HHP)treatment has attracted much interest and has been used in several studies on protein ***,the study aimed to investigate the effects of enzymatic hydrolysis by Corolase PP under different pressure treatments(0.1,100,200,and 300 MPa for 1-5 h at 50℃)on the emulsifying property,antioxidant activity,and bitterness of soybean protein isolate hydrolysate(SPIH).As observed,the hydrolysate obtained at 200 MPa for 4 h had the highest emulsifying activity index(47.49 m^(2)/g)and emulsifying stability index(92.98%),and it had higher antioxidant activities(44.77%DPPH free radical scavenging activity,31.12%superoxide anion radical scavenging activity,and 61.50%copper ion chelating activity).At the same time,the enhancement of emulsion stability was related to the increase of zeta potential and the decrease of mean particle *** addition,the hydrolysate obtained at 200 MPa for 4 h had a lower bitterness value and showed better *** study has a broad application prospect in developing food ingredients and healthy foods.

关键词： Soybean protein isolate High hydrostatic pressure Emulsification Antioxidant Bitter taste

On-demand engineerable visible spectrum by fine control of electrochemical reactions

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National Science Review 2024年第3期11卷 239-249页

作者： Qirong Liu Lei Liu Yongping Zheng min Li Baofu Ding Xungang diao Hui-ming Cheng Yongbing Tang Advanced Energy Storage Technology Research Center Shenzhen Institute of Advanced TechnologyChinese Academy of Sciences Institute of Technology for Carbon Neutrality Shenzhen Institute of Advanced Technology Chinese Academy of Sciences School of Energy and Power Engineering North University of China School of Resource Environment and Safety EngineeringHunan University of Science and Technology School of Energy and Power Engineering Beihang University Shenzhen Key Laboratory of Energy Materials for Carbon Neutrality Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenyang National Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences

Tunability of optical performance is one of the key technologies for adaptive optoelectronic applications,such as camouflage clothing, displays, and infrared shielding. High-precision spectral tunability is of great importance for some special applications with on-demand adaptability but remains challenging. Here we demonstrate a galvanostatic control strategy to achieve this goal, relying on the finding of the quantitative correlation between optical properties and electrochemical reactions within materials. An electrochromic electro-optical efficiency index is established to optically fingerprint and precisely identify electrochemical redox reactions in the electrochromic device. Consequently, the charge-transfer process during galvanostatic electrochemical reaction can be quantitatively regulated, permitting precise control over the final optical performance and on-demand adaptability of electrochromic devices as evidenced by an ultralow deviation of <3.0%. These findings not only provide opportunities for future adaptive optoelectronic applications with strict demand on precise spectral tunability but also will promote in situ quantitative research in a wide range of spectroelectrochemistry, electrochemical energy storage, electrocatalysis, and material chemistry.

关键词： electrochromics fine control spectral tunability high precision galvanostatic control

Stevens-Johnson综合征和中毒性表皮坏死松解症的并发症及处理

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中国皮肤性病学杂志 2019年第2期33卷 222-225页

作者：刁敏吕小岩四川大学华西医院四川成都610041

Stevens-Johnson综合征(Stevens-Johnson syndrome,SJS)和中毒性表皮坏死松解症(toxic epidermal necrolysis,TEN)是重症药疹,病情严重可威胁生命。SJS/TEN患者急性期并发症多见,包括感染、多脏器受累、体液丢失与电解质紊乱、低蛋白血... 详细信息

Stevens-Johnson综合征(Stevens-Johnson syndrome,SJS)和中毒性表皮坏死松解症(toxic epidermal necrolysis,TEN)是重症药疹,病情严重可威胁生命。SJS/TEN患者急性期并发症多见,包括感染、多脏器受累、体液丢失与电解质紊乱、低蛋白血症等。本文主要从SJS和TEN的并发症包括感染、内脏损害及眼部受累方面进行综述,并简述相应的处理原则。

关键词： Stevens-Johnson综合征中毒性表皮坏死松解症并发症感染抗生素

Design and experiment analysis of the small maize harvester with attitude adjustment in the hilly and mountainous areas of China

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International Journal of Agricultural and Biological Engineering 2024年第1期17卷 118-127页

作者： Meizhou Chen Guangfei Xu Yuanzhen Wei Yinping Zhang Peisong diao Huanxiao Pang College of Agricultural Engineering and Food Science Shandong University of TechnologyZibo255000ShandongChina School of Mechanical and Automotive Engineering Liaocheng UniversityLiaocheng252000ShandongChina Shandong Academy of Agricultural Machinery Sciences Jinan 250000China

To solve the problem of small planting plots and large sloping land for mechanized maize harvesting in China hilly and mountainous areas,a small maize harvester with attitude adjustment was designed to realize maize snapping,peeling,straw crushing and attitude adjusting at on time in this *** basic structure and working principle of the small maize harvester were described,and the key components were designed as *** maize snapping device adopted the combination form of maize snapping plates and straw pulling rollers,and the gap of the straw pulling rollers can be adjusted to adapt to different maize *** pairs of peeling rollers formed a groove arrangement to improve peeling rate and reduced ear grain *** pressure feeding device mainly comprised drive chain and three grade pressure feeding rollers to increase the friction between ears and the peeling rollers,and help ears *** attitude adjustment advice was designed according to the high point stationary pursuit leveling *** the attitude angle of the rack approached 0,the small maize harvester reached the level *** actual range of attitude adjustment was obtained and the accuracy of static attitude adjustment was verified through attitude adjustment *** influencing factors of ear loss rate and bract peeling rate were determined by orthogonal test,including the rotational speed of straw pulling rollers,peeling rollers and pressure feeding *** mathematical regression model between the experimental factors and indicators was established by using Design Expert,and through the analysis variance to verify the significance of the evaluation indicators,the best combination of operation parameters was determined that the rotational speed of straw pulling rollers,peeling rollers and pressure feeding rollers were 1440 r/min,1535 r/min and 406 r/*** the optimal combination of the operation parameters,the ear loss rate and bract peeling rate were 1.33%and 93.98%.T

关键词： maize harvester hilly and mountains areas attitude adjustment anti rollover structure

培养大学生社会主义核心价值观情感认同的逻辑理路

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山东青年政治学院学报 2019年第1期35卷 66-71页

作者：刁敏西南大学马克思主义学院重庆400715

大学生对社会主义核心价值观的认同是认知认同、情感认同和行为认同的有机统一。情感认同作为认知认同和行为认同的中介,是认知认同的进一步深化,也为行为认同的产生提供感情因素。一般而言,感觉、认知、体验和反应四个阶段是社会主义... 详细信息

大学生对社会主义核心价值观的认同是认知认同、情感认同和行为认同的有机统一。情感认同作为认知认同和行为认同的中介,是认知认同的进一步深化,也为行为认同的产生提供感情因素。一般而言,感觉、认知、体验和反应四个阶段是社会主义核心价值观情感认同得以生成的必经阶段,它们相互区别、相互联系。其中,感觉是情感认同生成的起点,认知是情感认同生成的基础,体验是情感认同生成的关键,行为反应是情感认同生成的表现。这在一定程度上也为培养大学生对社会主义核心价值观的情感认同提供了较为清晰的逻辑理路:通过环境刺激来引发大学生产生对社会主义核心价值观的主观感受;通过理论感召来奠定大学生对社会主义核心价值观的理性认知;通过情境创设来推进大学生切身体验社会主义核心价值观;通过实践强化来培养大学生对社会主义核心价值观的行为习惯。

关键词：大学生社会主义核心价值观情感认同培养理路