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Gelatin-Modified Biomimetic Apatite Coatings

Journal of Biomaterials and Nanobiotechnology 2012年第2期3卷 154-162页

作者： Barbara Bracci Paola Torricelli Silvia Panzavolta Katia Rubini milena fini Adriana Bigi Department of Chemistry “G. Ciamician” University of Bologna Bologna Italy Preclinical and Surgical Studies Laboratory Re- search Institute Codivilla Putti—Rizzoli Orthopaedic Institute Bologna Italy

Biomimetic coatings constituted of gelatin and nanocrystalline apatite were deposited on titanium substrates from a slightly supersaturated calcium phosphate (CaP) solution enriched with different amounts of gelatin. Although the biopolymer inhibits the crystallization of the inorganic phase, as shown by the reduction of the mean dimensions of the spherical aggregates and of the degree of crystallinity of the apatitic phase on increasing gelatin concentration, the deposition of a uniform layer of nanocrystalline apatite takes place in a few hours. Gelatin incorporation into the precipitate increases on increasing its concentration in solution, up to about 20 wt%. Osteoblast-like MG63 cells cultured on the coatings display good proliferation and increased values of the differentiation parameters with respect to the control. The presence of gelatin improves significantly the biological response to the biomimetic coatings, as shown by the higher values of proliferation, collagen type I and osteocalcin production, as well as alkaline phosphatase activity stimulation.

关键词： Biomimetic Coating Gelatin Hydroxyapatite

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Morphological Evaluation of Adhesion and Proliferation of Osteoblast Like Cells Grown on Gelatin/Genipin Scaffold

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Journal of Life Sciences 2013年第9期7卷 965-970页

作者： Gabriella Teti Adriana Bigi Monica Mattioli-Belmonte Roberto Giardino milena fini Antonio Mazzotti Mirella Falconi Department of Biomedical and Neuromotor Sciences University of Bologna Bologna 40126 Italy Department of Chemistry "G. Ciamician" University of Bologna Bologna 40126 Italy Department of Clinical and Molecular Sciences Universita Politecnica delle Marche Ancona 60121 Italy Laboratory of Preclinical Surgical Studies Rizzoli Orthopaedic Institute Bologna 40136 Italy Rizzoli Orthopaedic Institute IRCCS Bologna 40136 Italy

This work focusing on studying the biocompatibility and the effect of gelatin porous scaffold on the characteristics of human osteoblast like cells, including proliferation, adhesion, scaffold-cell interaction and its potential to induce bone regeneration. Osteoblast like cells were seeded on gelatin/genipin scaffolds for 7, 14 and 21 days. Cell proliferation assay, light microscopy, transmission electron microscopy and high resolution scanning electron microscopy were carried to evaluate cell viability, cell adhesion and the production of extracellular matrix. Cell proliferation assay showed a high biocompatibility of the material. High resolution scanning electron microscopy and light microscopy showed a strong adhesion of MG63 cells on the surface of gelatin scaffold and high penetration in the macroporosities of the material. TEM analysis showed an intense production of extracellular matrix protein. In vitro analysis indicated a good biocompatibility of the scaffold and presents it as a potential candidate material for tissue engineering.

关键词：成骨细胞样细胞细胞生长细胞增殖细胞粘附明胶评估扫描电子显微镜脚手架

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