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Laser-based in situ embedding of metal nanoparticles into bioextruded alginate hydrogel tubes enhances human endothelial cell adhesion

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Nano Research 2016年第11期9卷 3407-3427页

作者： Andreas Blaeser Nina Million Daniela Filipa Duarte Campos Lisa Gamrad Marius Kopf Christoph Rehbock milen nachev Bernd Sures Stephan Barcikowski Horst Fischer Department of Dental Materials and Biomaterials Research RWTH Aachen University Hospital Pauwelsstr. 30 52074 Aachen Germany Technical Chemistry I and Center for Nanointegration Duisburg-Essen (CENIDE) University of Duisburg-Essen Universitatsstr. 5-7 45141 Essen Germany Aquatic Ecology University of Duisburg-Essen Universitatsstr. 5 45141 Essen Germany Center for Water and Environmental Research University of Duisburg-Essen Universitatsstr. 5 45141 Essen Germany

Alginate is a widely used hydrogel in tissue engineering owing to its simple and non-cytotoxic gelation process, ease of use, and abundance. However, unlike hydrogels derived from mammalian sources such as collagen, alginate does not contain cell adhesion Iigands. Here, we present a novel laser ablation technique for the in situ embedding of gold and iron nanoparticles into hydrogels. We hypothesized that integration of metal nanoparticles in alginate could serve as an alternative material because of its chemical biofunctionalization ability （coupling of RGD ligands） to favor cell adhesion. Cytocompatibility and biofunctionality of the gels were assessed by cell culture experiments using fibroblasts and endothelial cells. Nanoparticles with an average particle size of 3 nm （gold） and 6 nm （iron） were generated and stably maintained in alginate for up to 6 months. Using an extrusion system, several centimeter-long alginate tubes with an outer diameter of approximately 3 mm and a wall thickness of approximately 150 μm were manufactured. Confocal microscopy revealed homogeneously distributed nanoparticle agglomerates over the entire tube volume. Endothelial cells seeded on iron-loaded gels showed significantly higher viability and an increased degree of spreading, and the number of attached cells was also elevated in comparison to the control and gold-loaded alginates. We conclude that laser-based in situ integration of iron nanoparticles （40.01 wt.%） in alginate is a straightforward method to generate composite materials that favor the adhesion of endothelial cells. In addition, we show that nanoparticle integration does not impair the alginate＇s gelation and 3D biofabrication properties.

关键词： biofabrication tissue engineering HUVEC nanocomposite laser ablation confocal microscopy additive manufacturing

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A Variational Principle and Best Proximity Points

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Acta Mathematica Sinica,English Series 2015年第8期31卷 1315-1326页

作者： milen IVANOV Boyan ZLATANOV Nadia ZLATEVA Faculty of Mathematics and Informatics Sofia University "St.Kliment Ohridski" 5James Bourchier blvd.1164 Sofia Bulgaria Faculty of Mathematics and Informatics Plovdiv University "Paisii Hilendarski"24 "Tsar Assen"str.4000 Plovdiv Bulgaria Faculty of Mathematics and Informatics Sofia University "St.Kliment Ohridski"5James Bourchier blvd.1164 Sofia Bulgaria

We generalize Ekeland＇s Variational Principle for cyclic maps. We present applications of this version of the variational principle for proving of existence and uniqueness of best proximity points for different class... 详细信息

关键词： Fixed point cyclical operator best proximity point uniformly convex Banach space variational principle

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世界音响之王BOSE——从美国剑桥小城发出的天籁之音

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城市地理 2009年第3期 76-81页

作者：袁婧 milen Bondar（图）

提及白富的第一座驾，我们自然会想到演绎美国领袖风范的凯迪拉克，忆及世界最豪华的音乐厅，巴塞罗那歌剧院便立即映入眼帘：谈及浦东“双龙戏珠”美景的上海东方明珠广播电视塔，就如传世之作般令我们无比骄傲。

关键词： BOSE 美国世界剑桥音响广播电视塔凯迪拉克东方明珠

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精神心脏罗马离众神最近的浪漫和激情

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城市地理 2009年第4期 72-79页

作者：曹雅欣 milen Bondar

“公主殿下，您对所访问的城市中印象最深的是哪一个呢？”“……罗马！无疑是罗马！这里的一切记忆，我都会珍惜，直到永远……”

关键词：罗马心脏精神激情浪漫记忆

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变革地带

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建筑实践 2015年第5期 12-19页

作者：李莹 Takuji Shimmura milenè Servelle

巴黎正慎重地允许高层建筑回归,Harnonic+Masson与Comte Vollenweider两家法国建筑事务所最近刚刚完成自上世纪70年代以来在巴黎建立的第一座达到50米的住宅项目。这象征着,人们愿意重新考虑在巴黎建造高层建筑的潜在可能性。2011年11... 详细信息

巴黎正慎重地允许高层建筑回归,Harnonic+Masson与Comte Vollenweider两家法国建筑事务所最近刚刚完成自上世纪70年代以来在巴黎建立的第一座达到50米的住宅项目。这象征着,人们愿意重新考虑在巴黎建造高层建筑的潜在可能性。2011年11月16日,星期二,在巴黎议会会议上,委员会的成员们修订了巴黎的城市法规,修正案将允许在巴黎东南部十三区马沙布鲁诺(Massena-Bruneseau)工业区建造50米高的住宅塔楼和高达180米的写字楼。既可以成为一栋独立建筑,也可以提供社会住房,项目齐整地连接起法兰西大道与更远处的铁路景观,成为通往伊夫里郊区的入口。

关键词：建筑师市议会铁路景观伊夫里

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小熊温尼·菩

小熊温尼·菩

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作者： A.A.·米尔恩(A.A. milen)

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Comparative and population genomics of buckwheat species reveal key determinants of flavor and fertility

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Molecular Plant 2023年第9期16卷 1427-1444页

作者： Kaixuan Zhang Yuqi He Xiang Lu Yaliang Shi Hui Zhao Xiaobo Li Jinlong Li Yang Liu Yinan Ouyang Yu Tang Xue Ren Xuemei Zhang Weifei Yang Zhaoxia Sun Chunhua Zhang Muriel Quinet Zlata Luthar Mateja Germ Ivan Kreft Dagmar Janovska Vladimir Meglic Barbara Pipan milen I.Georgiev Bruno Studer Mark A.Chapman Meiliang Zhou Institute of Crop Sciences Chinese Academy of Agricultural SciencesNational Crop Genebank BuildingZhongguancun South Street No.12Haidian DistrictBeijing 100081China College of Agronomy Sichuan Agricultural UniversityChengdu 611130China Annoroad Gene Technology(Beijing)Co. LtdBeijing 100176China College of Agriculture Institute of Agricultural BioengineeringShanxi Agricultural UniversityTaigu 030801ShanxiChina Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding Shanxi Agricultural UniversityTaiyuan 030031ShanxiChina Tongliao Institute Agricultural and Animal Husbandry Sciences Tongliao 028015Inner MongoliaChina Groupe de Recherche en Physiologie Ve´ge´tale(GRPV) Earth and Life Institute-Agronomy(ELI-A)Universite´Catholique de LouvainCroix du Sud 4-5bo^ıte L7.07.13B-1348Louvain-la-NeuveBelgium Biotechnical Faculty University of Ljubljana1000 LjubljanaSlovenia Nutrition Institute Trzaska 401000 LjubljanaSlovenia Gene Bank Crop Research InstituteDrnovska´507Prague 6Czech Republic Agricultural Institute of Slovenia Hacquetova ulicaLjubljanaSlovenia Laboratory of Metabolomics Institute of MicrobiologyBulgarian Academy of SciencesPlovdivBulgaria Center of Plant Systems Biology and Biotechnology PlovdivBulgaria Molecular Plant Breeding Institute of Agricultural SciencesETH ZurichUniversitaetstrasse 28092 ZurichSwitzerland Biological Sciences University of SouthamptonLife Sciences Building 85Highfield CampusSouthampton SO171BJUK

Common buckwheat(Fagopyrum esculentum)is an ancient crop with a world-wide *** to its excellent nutritional quality and high economic and ecological value,common buckwheat is becoming increasingly important throughout the *** availability of a high-quality reference genome sequence and population genomic data will accelerate the breeding of common buckwheat,but the high heterozygosity due to the outcrossing nature has greatly hindered the genome *** we report the assembly of a chromosome-scale high-quality reference genome of *** ***,a homozygous self-pollinating variant of common *** genomics revealed that two cultivated buckwheat species,common buckwheat(***)and Tartary buckwheat(***),underwent metabolomic divergence and ecotype *** expansion of several gene families in common buckwheat,including FhFAR genes,is associated with its wider distribution than Tartary *** number variation of genes involved in the metabolism of flavonoids is associated with the difference of rutin content between common and Tartary ***,we present a comprehensive atlas of genomic variation based on whole-genome resequencing of 572 accessions of common *** and evolutionary genomics reveal genetic variation associated with environmental adaptability and floral development between Chinese and non-Chinese cultivated ***-wide association analyses of multi-year agronomic traits with the content of flavonoids revealed that Fh05G014970 is a potential major regulator of flowering period,a key agronomic trait controlling the yield of outcrossing crops,and that Fh06G015130 is a crucial gene underlying flavor-associated ***,we found that the gene translocation and sequence variation of FhS-ELF3 contribute to the homomorphic self-compatibility of common ***,our results elucidate the genetic basis of spe

关键词： buckwheat genomics natural variation adaptation flavonoids Fagopyrum

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Unilateral 6-OHDA <i>th-fgfr1</i>(<i>tk-</i>) mouse model supports the role of FGFs in Parkinson’s disease and the effects of nicotine and L-DOPA on spontaneous motor impairments

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Health 2012年第11期4卷 1178-1190页

作者： Aaron Kucinski Scott Wersinger Ewa K. Stachowiak milen Radell Renae Hesse Thomas Corso Matthew Parry Merouane Bencherif Kristen Jordan Sharon Letchworth Michal K. Stachowiak Department of Biochemistry and Neuroscience Lake Erie College of Osteopathic Medicine Erie USA Department of Pathology and Anatomical Sciences State University of New York Buffalo USA Department of Pathology and Anatomical Sciences State University of New York Buffalo USA Targacept Inc. Winston-Salem USA

In the developing and adult brain, neurotrophic growth factors support the growth and protec tion of dopaminergic neuronal systems. Recently, links between impaired neurotrophin support of dopamine (DA) neurons has been described in Parkinson’s Disease (PD). Fibro- blast growth factor (FGF) has a unique association with DA neurons in that FGF signaling is vitally important for the development and protection of adult DA neurons. We assessed the role of substantia nigra (SN)-expressed FGFs in the nigrostriatal dopaminergic system using a transgenic mouse, th-fgfr1(tk-). In these mice, generated by expression of dominant negative FGFR1(TK-) from the tyrosine hydroxylase (TH) gene promoter, reduced FGF signaling results in smaller and less dense adult nigrostriatal DA neurons, similar to what is observed in PD. With unilateral 6-hydroxydopamine (6-OHDA) lesions, th-fgfr1(tk-) mice exhibited extensive unilateral nigrostriatal damage with robust spontaneous (non-drug induced) asymmetrical turning and a decreased latency to remain on the accelerating rotarod. L-DOPA remains the gold standard for PD therapy despite debilitating hyperkinetic and dyskinetic side effects. The nicotinic acetylcholine system has recently been targeted as an alternative system to combat PD motor symptoms. Nicotine effectively stimulates dopaminergic transmission in the nigrostriatal pathway and mediates movement. Using unilaterally lesioned th-fgfr1(tk-) mice, long term (11 day) oral administration of nicotine increased spontaneous bidirectional turning and increased the latency before falling from the accelerating rotarod. In a separate analysis, L-DOPA treatment reversed directionality of rotation and further deepened motor discoordination, suggesting activation of hypersensitive postsynaptic DA receptors in the denervated striata. These results in a transgenic model of PD provide insights into the cellular mechanisms underlying L-DOPA and nicotinic therapies and offer further evidence of nicot

关键词： Parkinson’s Disease Fibroblast Growth Factor (FGF) Signaling Transgenic Mouse Nicotine L-DOPA

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