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Protein and non-protein sulfhydryls and disulfides in gastric mucosa and liver after gastrotoxic chemicals and sucralfate: Possible new targets of pharmacologic agents

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World Journal of Gastroenterology 2007年第14期13卷 2053-2060页

作者： Lajos Nagy miki nagata Sandor Szabo Departments of Pathology Brigham & Women's Hospital Harvard Medical SchoolBostonMassachusetts 02115and Pathology and Laboratory Medicine ServiceDiagnostic & Molecular Medicine HCGVA Medical Center9901 East 7th St. Long BeachCA 90822United States Depts. of 3rd Internal Medicine and Family MedicineFaculty of MedicineUniversity of PecsH-7632 PecsHungary Departments of Pathology Brigham & Women's Hospital Harvard Medical SchoolBostonMassachusetts 02115and Pathology and Laboratory Medicine ServiceDiagnostic & Molecular Medicine HCGVA Medical Center9901 East 7th St. Long BeachCA 90822United States

AIM： To investigate the role of major non-protein and protein sulfhydryls and disulfides in chemically induced gastric hemorrhagic mucosal lesions （HML） and the mechanism of gastroprotective effect of sucralfate.METHODS： Rats were given 1 mL of 75% ethanol, 25%NaCl, 0.6 mol/L HCI, 0.2 mol/L NaOH or 1% ammonia solutions intragastrically （i.g.） and sacrificed 1, 3, 6 or 12 min later. Total （reduced and oxidized） glutathione （GSH ＋ GSSG）, glutathione disulfide （GSSG）, protein free sulfhydryls （PSH）, protein-glutathione mixed disulfides （PSSG） and protein cystine disulfides （PSSP） were measured in gastric mucosa and liver.RESULTS： Reduced glutathione （GSH） was depleted in the gastric mucosa after ethanol, HCI or NaCl exposure,while oxidized glutathione （GSSG） concentrations increased, except by HCI and NaOH exposure. Decreased levels of PSH after exposure to ethanol were observed,NaCl or NaOH while the total protein disulfides were increased. Ratios of reduced to oxidized glutathione or sulfhydrils to disulfides were decreased by all *** changes in thiol homeostasis were detected in the liver after i.g. abbreviation should be spelled out the first time here administration of ethanol. Sucralfate increased the concentrations of GSH and PSH and prevented the ethanol-induced changes in gastric mucosal thiol concentrations.CONCLUSION： Our modified methods are now suitable for direct measurements of major protein and nonprotein thiols/disulfides in the gastric mucosa or liver.A common element in the pathogenesis of chemically induced HML and in the mechanism of gastroprotective drugs seems to be the decreased ratios of reduced and oxidized glutathione as well as protein sulfhydryls and disulfides.

关键词： Non-protein and protein thiol Gastricmucosal injury Gastroprotection Sucralfate

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分子外流源L1211中近红外HH天体的发现(英文)

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天文学报 1999年第3期40卷 235-246页

作者：姚永强陈亚峰杨戟石井未来长田哲也佐藤修三中国科学院紫金山天文台中国科学院国家天文观测中心北京100012 日本名古屋大学

报告分子外流源Ｌ１２１１中新发现的１２个近红外ＨＨ天体．在Ｌ１２１１的～４’区域，取得了ＪＨＫ’宽带和Ｈ２　ｖ＝１０　Ｓ（１）发射线窄带的近红外图像．分析表明，新探测到的许多红外源与浓密分子云物理成协，其... 详细信息

报告分子外流源Ｌ１２１１中新发现的１２个近红外ＨＨ天体．在Ｌ１２１１的～４’区域，取得了ＪＨＫ’宽带和Ｈ２　ｖ＝１０　Ｓ（１）发射线窄带的近红外图像．分析表明，新探测到的许多红外源与浓密分子云物理成协，其中有２０多个源表现出典型的ＴＴａｕｒｉ星、ＨｅｒｂｉｇＡｅ／Ｂｅ星和原恒星的红外超．Ｌ１２１１中的ＩＲＡＳ点源没有被观测到，可能是更深地埋于分子云中．对红外观测和分子线观测消光估计的比较显示，小于２μｍ的近红外观测还不足以揭示深埋于分子云的整个年轻星团．根据近红外ＨＨ天体的形态分布和红外源性质，能进一步证认这些ＨＨ天体的激发源．其结果表明，Ｌ１２１１

关键词：红外成像 HH天体恒星形成分子外流源

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Hepatoma-derived growth factor expression as a prognostic marker in cervical cancer

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World Journal of Obstetrics and Gynecology 2015年第1期4卷 16-23页

作者： Misa Song miki Tomoeda Yu-Feng Jin Chiaki Kubo Hidenori Yoshizawa Masanori Kitamura Shigenori nagata Yukinobu Ohta Shoji Kamiura Hideji Nakamura Yasuhiko Tomita Department of Pathology Osaka Medical Center for Cancer and Cardiovascular Diseases Department of Gynecology Osaka Medical Center for Cancer and Cardiovascular Diseases Department of Internal Medicine Nissay Hospital

AIM： To examine the association of hepatoma-derived growth factor （HDGF） expression with the prognosis of patients with cervical cancer of the uterus （CC）. METHODS： HDGF is a unique nuclear growth factor, and it may play an important role in the development and progression of carcinoma. HDGF expression in 88 CC patients aged 23 to 76 years （median, 54 years） was analyzed by immunohistochemistry. A rabbit polyclonal antibody against the C-terminal amino acids （aa 231-240） of the human HDGF sequence was used as primary antibody at a dilution of 1：5000. This specific anti-HDGF antibody was purified using C-terminal peptide-conjugated Sepharose columns. Staining of endothelial cells in the noncancerous areas of each specimen was used as an internal positive control. Samples with more than 80% of tumor cells showing positive immunoreactivity in both the nucleus and cytoplasm were regarded as HDGF index level 2,more than 80% positive immunoreactivity in either the nucleus or cytoplasm as level 1, and less than 80% in both the nucleus and cytoplasm as level 0. The chi-square test and Fisher’s exact probability test were used to examine the relationship between HDGF expression and clinicopathologic parameters, and statistical significance was examined by the log-rank test. Multivariate analysis of factors related to survival was performed using Cox’s proportional hazards regression model. Statistical signifcance was set at P 〈 0.05. RESULTS： The five-year overall survival rate was 82.9%. Fourteen patients died due to tumors, nine of whom had tumor recurrence at 2-21 mo （median, 10 mo） after surgery. Tumor recurrence in five patients was determined at the time of the patients’ deaths. Nineteen cases were regarded as HDGF index level 0, 11 as level 1, and 58 as level 2. Patients with level 2 expression showed higher rates of histological classification of keratinized squamous cell carcinomaand adenosquamous carcinoma （44.8% of level 2 patients and 13.3% in levels 0 and 1）, de

关键词： Hepatoma-derived growth factor Prognosis Cervical cancer Immunohistochemical analysis Multiva-riate analysis

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