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Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway

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Synthetic and Systems Biotechnology 2022年第2期7卷 738-749页

作者： Francesca V.Gambacorta Ellen R.Wagner Tyler B.Jacobson Mary Tremaine Laura K.Muehlbauer Mick A.McGee Justin J.Baerwald Russell L.Wrobel John F.Wolters mike place Joshua J.Dietrich Dan Xie Jose Serate Shabda Gajbhiye Lisa Liu Maikayeng Vang-Smith Joshua J.Coon Yaoping Zhang Audrey P.Gasch Daniel Amador-Noguez Chris Todd Hittinger Trey K.Sato Brian F.Pfleger DOE Great Lakes Bioenergy Research Center University of Wisconsin-MadisonMadisonWIUSA Department of Chemical and Biological Engineering University of Wisconsin-MadisonMadisonWIUSA Laboratory of Genetics Center for Genomic Science InnovationUniversity of Wisconsin-MadisonMadisonWIUSA Department of Bacteriology University of Wisconsin-MadisonMadisonWIUSA Department of Chemistry University of Wisconsin-MadisonMadisonWIUSA Wisconsin Energy Institute J.F.Crow Institute for the Study of EvolutionUniversity of Wisconsin-MadisonMadisonWIUSA Department of Biomolecular Chemistry University of Wisconsin-MadisonMadisonWIUSA

Metabolic engineering strategies have been successfully implemented to improve the production of isobutanol,a next-generation biofuel,in Saccharomyces ***,we explore how two of these strategies,pathway re-localization and redox cofactor-balancing,affect the performance and physiology of isobutanol producing *** equipped yeast with isobutanol cassettes which had either a mitochondrial or cytosolic localized isobutanol pathway and used either a redox-imbalanced(NADPH-dependent)or redox-balanced(NADH-dependent)ketol-acid reductoisomerase *** then conducted transcriptomic,proteomic and metabolomic analyses to elucidate molecular differences between the engineered *** localization had a large effect on isobutanol production with the strain expressing the mitochondrial-localized enzymes producing 3.8-fold more isobutanol than strains expressing the cytosolic ***-balancing did not improve isobutanol titers and instead the strain with the redox-imbalanced pathway produced 1.5-fold more isobutanol than the balanced version,albeit at low overall pathway *** genomic analyses suggested that the poor performances of the cytosolic pathway strains were in part due to a shortage in cytosolic Fe-S clusters,which are required cofactors for the dihydroxyacid dehydratase *** then demonstrated that this cofactor limitation may be partially recovered by disrupting iron homeostasis with a fra2 mutation,thereby increasing cellular iron *** resulting isobutanol titer of the fra2 null strain harboring a cytosolic-localized isobutanol pathway outperformed the strain with the mitochondrial-localized pathway by 1.3-fold,demonstrating that both localizations can support flux to isobutanol.

关键词： Saccharomyces cerevisiae Isobutanol Functional genomics analysis Pathway localization Fe incorporation

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Don't Call Me Famous!From Reality TV to the Realities of a Life in Music;the Journey of Su Zixu

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城市漫步（GBA版） 2023年第5期 14-17页

作者： mike Fox 不详

Being a successful singer-songwriter comes with many *** from the pressure to write tunes thatarecontinuouslyworthy of an audience's attention;the determination,talent and mindset to get into that position in the firs... 详细信息

关键词： Famous get continuously

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Forest Mapping with ERS SAR Tandem Data

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Chinese Forestry Science and Technology 2002年第2期1卷 5-9页

作者： mike WOODING Franck RANERA Betlem ROSICH Remote Sensing Applications Consultants Ltd. 2 Prospect Place Mill Lane Alton GU34 2SX UKEarth Observation Application Department ESA/ESRIN Via Galileo Galilei 00044 Frascati ItalyEarth Observation Application Department ESA/ESRIN Via Galileo Galilei 00044 Frascati Italy

The feasibility of ERS SAR Tandem data for mapping forest and non-forest cover in China was evaluated over Zengcheng County in the South China. An accuracy of 75% has been achieved. Then, the MACFERST (Mapping China Forest with ERS SAR Tandem data) project started by the Ministry of Science and Technology (MOST) of China and the European Space Agency (ESA) in 1999. The generation of a large-scale forest map requires solving problems such as the georeferencing and mosaicking of very long image strips cov...

关键词： Forest mapping ERS SAR Tandem ILU image

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Significance of Shale Gas Development

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中国石油勘探 2009年第3期14卷 29-33页

作者： John Shelton mike D. Burnaman Wenwu Xia Nathaniel Harding Harding Shelton Group Oklahoma City Oklahoma 73102

Natural gas production from shale formations is growing exponentially in the United States. This paper introduces the five major shale formations in the United States and the technologies used to produce them. The Barnett, Haynesville, Fayetteville, Woodford, and Marcellus combined hold an estimated 978 trillion cubic feet of total gas reserves. These findings along with recent technological advances in horizontal drilling and completion methods have transformed the natural gas exploration and production industry in the U.S. and have fundamentally changed the U.S. energy picture. Specifically this paper states that the United States through the utilization of natural gas from shale can reduce its carbon emissions and can become more energy self-sufficient. Finally, the Harding & Shelton Group states in this paper that the opportunity to locate and produce shale gas in China is very similar to that which has taken place in the United States.

关键词：页岩石油天然气地质勘探

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事故模拟预防重大事故

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供用电 2014年第7期31卷 66-69页

作者： mike Toutloff

如果工作期间线路工人身受重伤或死亡，电力公司往往会派事故调查专家前往现场，以调查事故的根本诱因，从而避免今后再次发生类似的事故。事故现场会令工作人员及其家人痛心不已。同样，事故调查专家也会度过无数个无眠的夜晚，辗转反... 详细信息

如果工作期间线路工人身受重伤或死亡，电力公司往往会派事故调查专家前往现场，以调查事故的根本诱因，从而避免今后再次发生类似的事故。事故现场会令工作人员及其家人痛心不已。同样，事故调查专家也会度过无数个无眠的夜晚，辗转反侧，冥思他们亲眼目睹的严重事故。

关键词：重大事故事故模拟预防事故调查工作人员严重事故线路工现场会

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Measuring Pedestrian Stress Response (MPSR) Using Wearable Technologies

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Journal of Transportation Technologies 2024年第2期14卷 224-235页

作者： Ishita Dash Rachael Anne Muscatello Mark D. Abkowitz Ella R. Mostoller mike Sewell Department of Civil and Environmental Engineering Vanderbilt University 2301 Vanderbilt Place Nashville USA Department of Psychiatry and Behavioral Sciences SENSE Lab Vanderbilt University Medical Center 1211 Medical Center Drive Nashville USA Gresham Smith 111 West Main Street Suite 201 Louisville USA

Walkability is an essential aspect of urban transportation systems. Properly designed walking paths can enhance transportation safety, encourage pedestrian activity, and improve community quality of life. This, in turn, can help achieve sustainable development goals in urban areas. This pilot study uses wearable technology data to present a new method for measuring pedestrian stress in urban environments and the results were presented as an interactive geographic information system map to support risk-informed decision-making. The approach involves analyzing data from wearable devices using heart rate variability (RMSSD and slope analysis) to identify high-stress locations. This data-driven approach can help urban planners and safety experts identify and address pedestrian stressors, ultimately creating safer, more walkable cities. The study addresses a significant challenge in pedestrian safety by providing insights into factors and locations that trigger stress in pedestrians. During the pilot study, high-stress pedestrian experiences were identified due to issues like pedestrian-scooter interaction on pedestrian paths, pedestrian behavior around high foot traffic areas, and poor visibility at pedestrian crossings due to inadequate lighting.

关键词： Wearable Technology Walkability Built Environment Pedestrian Safety

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融合技术加速手机设计

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电子产品世界 2004年第09B期11卷 64-70页

作者： Bill Krenik mike Yonker 德州仪器无线高级架构经理

新技术的不断涌现,推动着手机的不断更新换代。曾经是简单语音为主的单模手机已被多模智能手机、无线PDA和多媒体产品所取代。目前蓝牙技术、WLAN以及全球定位业务等新技术正融合于手机,这给产品设计带来更多挑战,必须提供创新解决方案... 详细信息

新技术的不断涌现,推动着手机的不断更新换代。曾经是简单语音为主的单模手机已被多模智能手机、无线PDA和多媒体产品所取代。目前蓝牙技术、WLAN以及全球定位业务等新技术正融合于手机,这给产品设计带来更多挑战,必须提供创新解决方案,完成理想的构架设计,满足无线市场的设计要求,推动新技术不断融合到手机设计。

关键词：手机设计智能手机 WLAN 802.11无线局域网多模蓝牙单模无线市场业务产品

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融合技术加速手机设计

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电子产品世界 2004年第18期 64-66+68-70+9页

作者： Bill Krenik ,mike Yonker 德州仪器德州仪器无线高级架构经理无线高级架构经理

关键词：手机设计蓝牙技术 WLAN 融合技术

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Formulation development and characterization of cellulose acetate nitrate based propellants for improved insensitive munitions properties

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Defence Technology（防务技术） 2014年第2期10卷 92-100页

作者： Thelma MANNING Jeffrey WYCKOFF Carlton ADAM Eugene ROZUMOV Kenneth KLINGAMAN Viral PANCHAL Joseph LAQUIDARA mike FAIR John BOLOGNINI Keith LUHMANN Steve VELARDE Christine KNOTT Stephanie M.PIRAINO Kevin BOYD US Army RDECOM ARDECPicatinny Arsenal Alliiant Tech ATK RAFPRD AAP Naval Surface Warhead Center Indian Head Army Research Laboratory Aberdeen Proving Ground

Cellulose acetate nitrate(CAN) was used as an insensitive energetic binder to improve the insensitive munitions(IM) properties of gun propellants to replace the M1 propellant used in 105 mm artillery *** contains the energetic nitro groups found in nitrocellulose(NC),but also acetyl functionalities,which lowered the polymer's sensitivity to heat and shock,and therefore improved its IM properties relative to *** formulation,development and small-scale characterization testing of several CAN-based propellants were *** formulations,using insensitive energetic solid fillers and high-nitrogen modifiers in place of nitramine were *** small scale characterization testing,such as closed bomb testing,small scale sensitivity,thermal stability,and chemical compatibility were *** mechanical response of the propellants under high-rate uni-axial compression at,hot,cold,and ambient temperatures were also *** diameter testing,hot fragment conductive ignition(HFCI) tests were done to evaluate the propellants' responses to thermal and shock *** the propellant chemical composition,theoretical predictions of erosivity were *** the small scale test results were utilized to down-select the promising CAN based formulations for large scale demonstration testing such as the ballistic performance and fragment impact testing in the105 mm M67 artillery charge *** test results completed in the small and large scale testing are discussed.

关键词：硝酸纤维素弹道性能钝感弹药推进剂醋酸纤维素开发配方特性测试

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祖鲁王国的衰亡

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英语学习（新锐空间） 2009年第3期 48-51页

作者： mike Pilewski 冰尘(译)

骁勇善战的祖鲁人为反抗英国殖民者的侵略展开了英勇悲壮的斗争。虽然最后是以祖鲁王国的失败而告终，却在非洲人民反抗殖民侵略的斗争史上谱写下了一页可歌可泣的光辉篇章……

关键词：王国衰亡殖民侵略英国殖民者斗争史反抗悲壮

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