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Clones identification of Sequoia sempervirens (D. Don) Endl. in Chile by using PCR-RAPDs technique

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第2期10卷 112-119页

作者： Manuel TORALIBAEZ Margarita CARU miguel A. HERRERA Luis gonzalez Luis M. MARTIN Jorge MIRANDA Rafael M. NAVARRO-CERRILLO Department of Forest Management Faculty of ForestryUniversity of Chile Santa Rosa 11315La PintanaSantiagoChile Department of Ecological Sciences Faculty of SciencesUniversity of ChileSanta Rosa 11315La PintanaSantiagoChile Department of Forestry School of Agriculture and Forestry University of Córdoba14080 CórdobaSpain Department of Genetic School of Agriculture and ForestryUniversity of Córdoba14080 CórdobaSpain Department of Forest Management Faculty of ForestryUniversity of ChileSanta Rosa 11315La PintanaSantiagoChile

A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction. The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was con- structed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA). Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of 49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although they are derived from the same geo- graphic origin.

关键词： Sequoia sempervirens (D. Don) Endl. Random amplified polymorphic DNAs (PCR-RAPDs) Molecular markers Clone assays Chile Genetic relationships

Parental perception of child's weight,their attitudes towards child's dietary habits and the risk of obesity

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World Journal of Pediatrics 2022年第7期18卷 482-489页

作者： Lorena Garcia-Blancol Arantxa Berasaluce Andrea Romanos-Nanclares miguel Angel Martinez-gonzalez Laura Moreno-Galaraga Nerea Martin-Calvo San Juan Primary Care Health Center.Servicio Navarro de Salud-Osasunbidea PamplonaSpain IdisNA.Instituto de Investigacion Sanitaria de Navrra.Pamplona Spain Department of Preventive Medicine and Public Health School of Medicine.University of NavarraC/Irunlarrea l.31080 Pamplona.Spain Biomedical Research Centre Network On Obesity and Nutrition(CIBERobn).Physiopathology of Obesity and NutritionInstituteof Health Carlos Ⅲ.Av.Monforte de Lemos.3-528029 MadridSpain Department of Nutrition.Harvard T.H.Chan School of Public Health Boston.MA 02115.USA Department of Pediatrices.Complejo Hospialario de Navarra B.Pamplona.Spain

Background The association between parental perception of child's weight and their attitudes towards his/her dietary habits has not been reported *** study aimed to assess the association between parental underestimation of child's weight and parental attitudes towards child's dietary *** We conducted a cross-sectional analysis of SENDO cohort participants recruited between January 2015 and June *** information was collected through online questionnaires completed by *** calculated crude and multivariable-adjusted odds ratio(OR)and 95%confidence intervals(CI)for unhealthy attitudes towards child's dietary habits associated with parental underestimation of child's *** Sixteen percent of children in the SENDO project had parents who underestimated their *** who under-estimated their child's weight status were more likely to have unhealthy attitudes toward his/her dietary habits[OR 3.35;95%CI(1.71-6.53)].Conclusions Parental underestimation of child's weight was associated with unhealthy attitudes towards child's dietary *** and public health practitioners should pay attention to the parental perception of child's weight to identify parents who underestimate it as an at-risk group in which to inquire about lifestyle and dietary habits.

关键词： Childhood obesity Dietary atitudes Eating habits Overweight Weight perception

A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway ComDonents

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Molecular Plant 2016年第1期9卷 136-147页

作者： Marta Peirats-Llobet Soon-Ki Han miguel gonzalez-Guzman Cheol Woong Jeong Lesia Rodriguez Borja Belda-Palazon Doris Wagner Pedro L. Rodriguez Instituto de Biologia Molecular y Celular de Plantas Consejo Superior de Investigaciones Cientfficas-Universidad Politecnica de Valencia 46022 Valencia Spain Department of Biology University of Pennsylvania Philadelphia PA 19104 USA These authors contributed equally to this article

Optimal response to drought is critical for plant survival and will affect biodiversity and crop performance during climate change. Mitotically heritable epigenetic or dynamic chromatin state changes have been implicated in the plant response to the drought stress hormone abscisic acid （ABA）. The Arabidopsis SWI/SNF chromatin-remodeling ATPase BRAHMA （BRM） modulates response to ABA by preventing pre- mature activation of stress response pathways during germination. We show that core ABA signaling pathway components physically interact with BRM and post-translationally modify BRM by phosphoryla- tion/dephosphorylation. Genetic evidence suggests that BRM acts downstream of SnRK2.2/2.3 kinases, and biochemical studies identified phosphorylation sites in the C-terminal region of BRM at SnRK2 target sites that are evolutionarUy conserved. Finally, the phosphomimetic BRMs17s^D S1762D mutant displays ABA hypersensitivity. Prior studies showed that BRM resides at target loci in the ABA pathway in the presence and absence of the stimulus, but is only active in the absence of ABA. Our data suggest that SnRK2- dependent phosphorylation of BRM leads to its inhibition, and PP2CA-mediated dephosphorylation of BRM restores the ability of BRM to repress ABA response. These findings point to the presence of a rapid phosphorylation-based switch to control BRM activity; this property could be potentially harnessed to improve drought tolerance in plants.

关键词： abscisic acid hormone signaling chromatin remodeling

Synthesis and Characterization of Green Potassium Nanoparticles from Sideroxylon Capiri and Evaluation of Their Potential Antimicrobial

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Journal of Renewable Materials 2021年第10期9卷 1699-1706页

作者： Judith Constantino-Alcazar miguel Abud-Archila Benjamín Valdez-Salas Federico Gutierrez-Miceli Carlos Ceceña-Duran Blanca López-Valenzuela Daniel gonzalez-Mendoza Tecnológico Nacional de México Instituto Tecnológico de Tuxtla GutiérrezTuxtlaGutiérrezC.P.03940México Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California(ICA-UABC) Ejido Nuevo LeónC.P.21705México Instituto de Ingeniería de la Universidad Autónoma de Baja California Mexicali21100México

In the present study,the green synthesis of potassium nanoparticles(K-NPs)was assessed using aqueous extract of Sideroxylon *** potassium nanoparticles were analyzed by UV-visible spectroscopic techniques,X-ray spectrometers of energy dispersive(SEM-EDS)and dynamic light *** results showed high values at 3.5 keV confirming the formation of potassium nanoparticles and the SEM analysis showed an agglomerated particles size between 360 to 200 nm with a spherical *** K-NPs showed an effective antibacterial activity against the test organisms mainly with Bacillus cereus,Enterobacter aerogenes,Fusarium solani and Botrytis *** further studies about nanotoxicity of K-NPs are needed to confirm their potential in the control of the pathogen microorganisms under field conditions.

关键词： Antimicrobial effect green synthesis Sideroxylon capiri biocontrol

Intestinal preparation prior to capsule endoscopy administration

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World Journal of Gastroenterology 2008年第37期14卷 5773-5775页

作者： Vicente Pons Beltrán Cristina Carretero Begoa gonzalez-Suárez Iaqui Fernández-Urien miguel Muoz-Navas Gastroenterology Department Hospital La FeValencia 46009 Spain Gastroenterology Department University of NavarraPamplona 31008 Spain Gastroenterology Department Hospital del MarBarcelona 08036 Spain

In order to have an adequate view of the whole small intestine during capsule endoscopy, the preparation recommended consists of a clear liquid diet and an overnight fast. However, visualization of the small bowel during video capsule endoscopy can be impaired by intestinal contents. To improve mucosal visualization, some authors have evaluated different regimens of preparation. There is no consensus about the necessity of intestinal preparation for capsule endoscopy and it should be interesting to develop adequate guidelines to improve its efficacy and tolerability. Moreover, the effect of preparation type (purgative) on intestinal transit time is not clear. Since a bowel preparation cannot defini-tively improve its visibility (and theoretically the yield of the test), it is not routinely recommended.

关键词： Capsule endoscopy Intestinal preparation Polyethylene glycol Aqueous sodium phosphate

Long-Term Postoperative Perfusion Indices in Surgically Resolved Myopic Traction Maculopathy

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Open Journal of Ophthalmology 2023年第1期13卷 143-171页

作者： miguel A. Quiroz-Reyes Erick A. Quiroz-gonzalez miguel A. Quiroz-gonzalez Virgilio Lima-Gomez Oftalmologia Integral ABC Retina Specialists Unit Medical and Surgical Assistance Institution (Nonprofit Organization) Affiliated with the Postgraduate Studies Division at the National Autonomous University of Mexico Mexico City Mexico Institute of Ophthalmology Fundacion Conde de Valenciana Private Assistance Institution (Nonprofit Organization) Mexico City Mexico Juarez Hospital Public Assistance Institution (Nonprofit Organization) Mexico City Mexico

Background: Pathological myopia can be complicated by the presence of posterior staphyloma, macular atrophy, ruptures in Bruch’s membrane, pathologic choroidal neovascularization, and different degrees of myopic traction maculopathy. Purpose: To report the structural, functional and perfusional outcomes in patients underwent surgery for different stages of myopic traction maculopathy (MTM). Methods: A retrospective, consecutive, comparative, interventional, one-surgeon, case-control study was conducted in 46 eyes of 34 individuals between April 2015 and May 2021. Participants included normal emmetropic eyes (Control emmetropia, n = 25), healthy myopic eyes (Control high myopia, n = 20), and operated and structurally fully resolved myopic eyes with different stages of MTM (Surgically treated group, n = 46). Long-term postoperative functional and perfusional follow-up evaluations were performed with spectral domain-optical coherence tomography (SD-OCT) and OCT angiography. The primary outcome measure included long-term functional, structural and perfusion macular status across groups. Results: Forty-six eyes in 34 patients were included in the study group, with both eyes affected in 12 (26.3%) patients. The mean axial length was 29.89 ± 1.67 mm. The preoperative logMAR was 1.29 ± 0.54 and the postoperative logMAR was 0.60 ± 0.52 (P Conclusion: Compared to emmetropic and healthy myopic eyes, surgically-resolved MTM eyes generally have larger superficial foveal avascular zone area, lower vessel density, smaller choriocapillaris flow area, thinner central subfoveal thickness, and more macular defects. Eyes with stage III or IV MTM had larger deviation compared to eyes at earlier stages. Visual function change after surgery was associated with superficial foveal avascular zone area. Better functional, structural and perfusion index outcomes were observed when highly myopic eyes underwent early surgery.

关键词： Choriocapillaris Flow Area Deep Vascular Plexus Foveoretinal Detachment Myopic Foveoschisis Foveal Avascular Zone Myopic Macular Hole

Surgical Approaches in Primary Rhegmatogenous Retinal Detachment: A Systematic Review and Meta-Analysis Comparing Vitrectomy vs. Vitrectomy Combined with Scleral Buckling, Lens-Sparing vs. Phako Procedures

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Open Journal of Ophthalmology 2023年第4期13卷 371-397页

作者： miguel A. Quiroz-Reyes Erick A. Quiroz-gonzalez miguel A. Quiroz-gonzalez Virgilio Lima-Gomez Retina Department of Oftalmologia Integral ABC Medical and Surgical Assistance Institution (Nonprofit Organization) Affiliated with the Postgraduate Studies Division at the National Autonomous University of Mexico Mexico City Mexico Institute of Ophthalmology Medical and Surgical Assistance Institution (Nonprofit Organization) Affiliated with the Postgraduate Studies Division at the National Autonomous University of Mexico Mexico City Mexico Juarez Hospital Public Assistance Institution (Nonprofit Organization) Mexico City Mexico

Aim: This study aimed to assess and compare the functional and anatomical results of pars plana vitrectomy (PPV) alone versus PPV combined with scleral buckling (SB), and lens-sparing versus phaco-procedures for treating rhegmatogenous retinal detachment. Methods: A comprehensive literature search was performed using the Web of Science, MEDLINE, EMBASE, and Cochrane Library databases to retrieve comparative studies. The main objective was to assess the BCVA, while reattachment rates and ocular adverse events were considered secondary measures. Rev Manager software was used for statistical analysis. Results: The literature search identified 10 articles comprising 1518 eyes, with 682 eyes in the PPV group, 193 eyes in the lens-sparing versus phaco-procedure group, and 643 eyes in the combined PPV and SB surgery group. Quality assessment revealed a low risk of bias in most domains. The meta-analysis results revealed a significant difference in postoperative BCVA between the PPV and PPV combined with SB groups (WMD = −0.17, 95% CI [0.27, 0.07], p = 0.001). The primary reattachment rates were 82.80% for PPV alone and 87.52% for PPV combined with SB (p = 0.34). The final reattachment rates did not differ significantly between PPV and PPV combined with SB (99% vs. 99.8%;RR = 1.00, 95% CI [1.01, 0.99], p = 0.96). PPV alone demonstrated a significantly reduced risk of macular edema compared to PPV combined with scleral buckling (9.9% vs. 23%;p = 0.006). The incidences of macular hole development (p = 0.46), recurrent retinal detachment (p = 0.27), proliferative vitreoretinopathy development (p = 0.48), epiretinal membrane proliferation (p = 0.77), and limited choroidal hemorrhage (p = 0.69) were not significantly different between the two groups. Conclusions: These findings suggest that PPV alone may be a more effective treatment option in terms of visual acuity (VA) improvement, lower risk of macular edema and cataract development. However, there was no significant differen

关键词： Lens-Sparing Phaco-Procedure Pars Plana Vitrectomy Rhegmatogenous Retinal Detachment Scleral Buckle

Resolving Myopic Foveoretinal Detachment by Fovea-Saving Internal Limiting Membrane Peeling: A Case-Series Report

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Open Journal of Ophthalmology 2023年第1期13卷 106-121页

作者： miguel Angel Quiroz-Reyes Erick Andres Quiroz-gonzalez miguel Angel Quiroz-gonzalez Virgilio Lima-Gomez Oftalmologia Integral ABC Retina Department Medical and Surgical Assistance Institution (Nonprofit Organization) Affiliated with the Postgraduate Studies Division at the National Autonomous University of Mexico Mexico City Mexico Juarez Hospital Public Assistance Institution (Nonprofit Organization) Mexico City Mexico

Background: In highly myopic eyes, myopic foveoschisis (MF), the earliest stage of myopic traction maculopathy (MTM), is present in up to 34% of patients with pathologic myopia and slowly progresses to form foveoretinal detachment (FRD) or macular hole (MH) with or without macular hole retinal detachment (MHRD) as a part of its natural history. Aim: To describe the microstructural and functional results in three highly myopic eyes that underwent macular surgery for early-stage MTM. The last postoperative structural findings were correlated with the final vision and macular automated microperimetry evaluation. Methods: We retrospectively reviewed three highly myopic eyes that underwent successful fovea-saving internal limiting membrane (FS-ILM) macular surgery for chronic FRD at Oftalmologia Integral ABC, Mexico City, Mexico. We performed postoperative multimodal microstructural and functional evaluations, including SD-OCT, SS-OCT, and microperimetric macular examinations. Results: There was a substantial difference between best-corrected visual acuity (BCVA) preoperatively and postoperatively in all three cases. Postoperative surgery was associated with significant improvement in visual acuity confirmed using a paired-sample permutation test. The mean presurgical BCVA value (LogMAR;mean ± SE) was ~0.83 ± 0.15, and the postsurgical value was ~0.43 ± 0.52 (P = 0.00065). The myopic foveoretinal detachment evaluation was ~7.3 ± 3.5 months, with a mean postoperative follow-up time of ~14 ± 4.08 months. Furthermore, postoperative multimodal imaging tests demonstrated an abnormal microstructural foveal SS-OCT pattern without evidence of macular hole (MH) development at the postoperative follow-up. Macular microperimetry confirmed a subclinical reduced macula threshold sensitivity with an anomalous retinal sensitivity analysis map and a stable central foveal fixation site. Conclusions: Even with the successful microstructural disappearance of myopic macular detachment, the

关键词： Automated Microperimetry Myopic Foveoretinal Detachment Myopic Foveoschisis Myopic Macular Hole Myopic Traction Maculopathy Posterior Staphyloma

Comparison of clinical outcomes between unrelated single umbilical cord blood and"ex-vivo"T-cell depleted haploidentical transplantation in children with hematological malignancies

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World Journal of Pediatrics 2021年第6期17卷 609-618页

作者： Carmen Gomez-Santos Marta gonzalez-Vicent Blanca Molina Natalia Deltoro Blanca Herrero Julia Ruiz Antonio Perez-Martinez miguel A Diaz Department of Pediatrics Hematopoietic Stem Cell Transplantation UnitHospital Infantil Universitario"Nino Jesus"Menedez Pelayo 6528009 MadridSpain Hospital Infantil Universitario"La Pa"Madrid MadridSpain

Background Over the last two decades,umbilical cord blood(UCB)and haploidentical transplantation(HaploHSCT)have emerged as alternative sources of hematopoietic stem cell for allogeneic *** are few retrospective studies and no prospective studies comparing both types of alternative transplantation in pediatric *** We analyzed the data of 134 children with hematological malignancies who received a hematopoietic stem cell transplantation from a single umbilical cord blood(UCB)(n=42)or an"tex-vivo"T-cell depleted transplant from a haploi-dentical-related donor(HaploHSCT)(n=92)between 1996 and *** recovery was faster after HaploHSCT than the UCB transplant group(median times to neutrophil and platelet recovery:13 vs.16 days,10 vs.57 days,respectively)(P<0.001).The HaploHSCT group had a significantly early immune reconstitution based on NK and CD8+T cells compared with the UCB ***,after the first year *** had a lower number of CD4+ T and B lymphocytes compared with the UCB transplant *** cumulative incidence of TRM was 29±8%in the HaploHSCT group versus 40±5%in the UCB *** incidence was 21±7%in the HaploHSCT group and 19±8%in the UCB *** of DFS was 58±8%in the HaploHSCT group versus 40±9%in the UCB group(P=0.051).Conclusions TCD haploidentical transplant is associated with advantages in terms of engraftment and early immune reconstitution *** haploidentical transplant was associated with lower incidence of infectious and non-infectious complications,especially in the early phases of the transplant compared with UCB transplant ***,there are no advantages in transplant outcomes compared with UCB transplant.

关键词： Children Cord blood transplant Hematologic malignancies Immune reconstitution TCD haploidentical transplant