检索结果-南通市图书馆

Estimation of Contraction Coefficient of Gorlin Equation for Assessment of Aortic Valve Area in Aortic Stenosis

World Journal of Cardiovascular Diseases 2017年第4期7卷 119-130页

作者： Ricardo A. Migliore María E. Adaniya miguel barranco Guillermo Miramont Silvia Gonzalez Horacio Tamagusuku Laboratory of Echocardiography Department of Cardiology Hospital Eva Perón San Martín Argentina

Background: The Gorlin equation is the reference method for the assessment of aortic valve area in aortic stenosis and is calculated using a constant, called the coefficient of contraction, which is empirically assumed to be 1. This coefficient is the ratio of effective aortic area to anatomic aortic area, and a value of 1 indicates that both are the same. The purpose of this study was to estimate the actual coefficient of contraction in patients with aortic stenosis and to evaluate its impact on aortic area as calculated by the Gorlin equation. Methods: We studied 17 patients with moderate to severe aortic stenosis. Effective aortic area was calculated using the continuity equation. Anatomic aortic area was obtained by planimetry with transesophageal echocardiography. Aortic valve area by the Gorlin equation was calculated from echocardiography data. The coefficient of contraction was derived as above. Results: The coefficient of contraction was inversely related to the pressure recovery. Effective area was correlated with anatomic area (r = 0.86, P 2). Aortic area by the Gorlin equation was not correlated with anatomic area, but the correlation became significant when the Gorlin equation was corrected for coefficient of contraction and pressure recovery. Conclusions: Using a coefficient of contraction of 1 in the Gorlin equation gives a poor correlation with anatomic area. Using the calculated coefficient of contraction for each patient and the mean gradient for pressure recovery improves the correlation with anatomic area. These facts could be taken in account when Gorlin equation is considered as the reference method.

关键词： Aortic Stenosis Echocardiography Hemodynamics Assessment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Enzymes of Entomopathogenic Fungi, Advances and Insights

引用

Advances in Enzyme Research 2014年第2期2卷 65-76页

作者： Lluvia de Carolina Sánchez-Pérez Juan Esteban barranco-Florido Silvia Rodríguez-Navarro José Francisco Cervantes-Mayagoitia miguel ángel Ramos-López Departamento de Producción Agrícola y Animal Universidad Autónoma Metropolitana México City México Departamento de Sistemas Biológicos Universidad Autónoma Metropolitana México City México Facultad de Química Universidad Autónoma de Querétaro Querétaro México

Entomopathogenic fungi (EF) are recognized biological control agents of insects. Basically, the entomopathogenic fungi pathogen activity depends on the ability of its enzymatic equipment, consisting of lipases, proteases and chitinases, which are in charge of breaking down the insect’s integument. Lipases are the first enzymes synthesized by the entomopathogenic fungi. Recently, a cytochrome P450 subfamily, referred as CYP52XI and MrCYP52 has been identified in Beauveria bassiana and Metarhizium robertsii, respectively. These break down long-chain alkenes and fatty acids to become initial nutrients. Subsequently, subtilisin type (Pr1) proteases sintetize;these enzymes are considered as virulence indicators and they are regulated by a signal transduction mechanism activated by the protein kinase A (PKA) mediated by AMPc. Through the employment of genetic engineering, it has been possible to increase virulence producing Pr1 recombinants with Androctonus australis neurotoxins or with chitinases, reducing the insect’s time of death. In the course of time, the Pr1 protease gene has presented evolutionary adaptations by gene duplication or horizontal transfer infecting different orders of insects. In the same way, the entomopathogenic fungi chitinases have presented a functional diversification. Currently, these have been phylogenetically classified into three subgroups, in accordance to the catalytic site domain and the chitin binding domain. The chitinolytic activity has increased through a directed evolution processes and genetic recombination with Bombyx mori chitinase. Recently, enzymes have been employed as control agents for insects and phytopathogenic fungi (disease originator) opening new potentialities in order to improve the entomopathogenic fungi use. Solid state fermentation is a bioprocess that would produce at great scale enzymes and some other metabolites in grade of increasing the entomopathogenic fungi virulence, in the control of insects and potentially

关键词： Lipases Proteases Chitinases Host Evolution Biocontrol Agent

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Assessment of Beauveria bassiana and Their Enzymatic Extracts against Metamasius spinolae and Cyclocephala lunulata in Laboratory

引用

Advances in Enzyme Research 2016年第3期4卷 98-112页

作者： Lluvia Sánchez-Pérez Silvia Rodríguez-Navarro Victor Hugo Marín-Cruz miguel Ángel Ramos-López Alejandro Palma Ramos Juan Esteban barranco-Florido Masters Program in Agricultural Sciences Division of Biological and Health Metropolitan Autonomous University-Xochimilco Coyoacán México Departament of Agricultural and Animal Production Metropolitan Autonomous University-Xochimilco Coyoacán México Faculty of Chemistry Autonomous University of Queretaro Santiago de Querétaro México Department of Biological Systems Metropolitan Autonomous University-Xochimilco Coyoacán México

The application of enzymatic extracts and conidia of Beauveria bassiana in Metamasius spinolae and Cyclocephala lunulata was evaluated. The variables were mortality and time of death. In M. spinolae, mortality with extracts 29%, conidia 27% and the combination of both 31%, all had a time of death of four days. Although with different symptoms, used enzymatic extracts: contraction and softening of the joints;by conidia: mycelium in the joints;in the combination of conidia and enzymatic extracts: abundant aerial mycelium. In C. lunulata, 100% mortality in all treatments;Time of death: enzymatic extracts and extracts with conidia 1.2 days;conidia 2.8 days. Symptoms were different, enzymatic extracts: melanization and internal tissue lysis;enzymatic extract and conidia: mycelium emerged and melanization;conidia: mycelium emerged. Enzymatic extracts showed insecticidal activity in M. spinolae and C. lunulata. These results suggest the potential of enzymatic extracts as biocontrol agents to improve the use of entomopathogenic fungi.

关键词： Insecticidal Activity Beauveria bassiana Metamasius spinolae Cyclocephala lunulata Enzymatic Extracts

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

<i>In Vitro</i>Characterization of the Efficacy and Safety Profile of a Proprietary Ajuga Turkestanica Extract

引用

Chinese Medicine 2012年第4期3卷 215-222页

作者： José M. Zubeldia Aarón Hernández-Santana miguel Jiménez-del-Rio Verónica Pérez-López Rubén Pérez-Machín José Manuel García-Castellano Department of Orthopaedic Surgery Complejo Hospitalario Universitario Insular Las Palmas Spain Molecular Oncology Group (G-OncoMol) Research Unit University Hospital of Gran Canaria Canary Health and Research Foundation Barranco de la Ballena Las Palmas Spain Polinat S. L. Taibique 4 Polígono Industrial Las Majoreras Las Palmas Spain

Ajuga Turkestanica, an herbaceous flowering species in the mint family, has been traditionally used in Turkeyand Uzbekistan for heart disease, muscle aches and stomach problems. Due to its high levels of phytoecdysteroids (particularly the characteristic C-11-hydroxylated Turkesterone), anabolic properties have also been reported. The aim of our study was to screen for early signs of efficacy and safety of a proprietary Ajuga turkestanica extract (ATE) using in vitro models. C2C12 mouse myotube cell line was used to study potential effects on viability and gene modulation. Cell viability was evaluated with different concentrations [0.2 - 200 ppm (mg/L)] of ATE. Gene modulation was assessed by quantitative polymerase chain reaction (qRT-PCR) after 6h incubation (ATE vs. the androgenic anabolic steroid methandrostenolone). Total androgenic activity was measured using the A-SCREEN bioassay. Ultra-high performance liquid chromatography analysis showed good correlation between the phytochemical profile of the native plant and our ATE. C2C12 mouse myotube cells treated with ATE experienced no significant loss of viability (concentrations 0.2 - 200 ppm, 1 - 24 hs, p > 0.05). qRT-PCR array analysis showed significant (p regulation of Caspase-3 (2-fold) and Myostatin (4-fold). The extract showed no androgenic activity within the dose range used. Our results indicate the potential for an ATE to support muscle mass without typical androgenic side effects of synthetic anabolic drugs.

关键词： Ecdysteroids Ajuga Turkestanica Turkesterone Caspase 3 Myostatin Androgenic Activity Sarcopenia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Analysis of Behaviour of Phase S Capacitive Tap Insulator of Interconnection 1,220 kV, in Jinamar Power Plant （Island of Gran Canaria, Canary Islands, Spain）

引用

材料科学与工程（中英文B版） 2013年第6期3卷 400-408页

作者： Jose Manuel Garcia Munoz Jose Antonio Torres Santana miguel Martinez Melgarejo Endesa S.A Las Palmas de Gran Canaria 35008 Spain Edificio de lngenierias Departamento de Ingenieria Electronica y Automatica University of Las Palmas de Gran Canaria (ULPGC) Las Palmas de Gran Canaria35017 Spain Edificio de Ingenierias Departamento de lngenieria Electrica University of Las Palmas de Gran Canaria (ULPGC) Las Palmasde Gran Canaria 35017 Spain

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：