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Overlapping of Laser Pulses and its Effect on the Yield of Silver Nanoparticles in Water

材料科学与工程（中英文B版） 2014年第10期4卷 279-283页

作者： David Omar Oseguera Galindo Israel Ceja Andrade Alejandro Martinez Benitez Gilberto Gomez Rosas Arturo Chavez-Chavez Perez Centeno miguel angel santana aranda Departamento de Materiales Avanzados Centro de Nanociencias y Nanotecnologia Universidad Nacional Autonoma de MexicoEnsenada Baja California 22860 Mexico Departamento de Fisica Centro Universitario de Ciencias Exactas e Ingenierias Universidad de Guadalajara GuadalajaraJaliseo 44430 Mexico Centro de lnvestigaciones en Optica Leon Guanajuato 37150 MOxico

Laser pulse overlapping is one of the least studied parameters involved in the production of nanoparticles by laser ablation confined in liquids. In order to explore its effect on the yield of nanoparticles, we employed a motorized XY translation stage with speed control, which allowed us to regulate the overlapping of laser pulses on the target. Four different XY speed settings were used in the laser ablation of a silver target in distilled water; each process in a virgin surface, using the 532 nm emission of a Nd: YAG laser, with fixed spot diameter of 1 mm and pulse energy of 0.5 J/pulse. Peak optical absorption, in UV-Visible measurements of each nanoparticle suspension, decreases as the employed pulse overlapping increases. According to the well known Beer-Lambert relation, this implies that nanoparticle yield decreases as the pulse overlapping increases.

关键词：银纳米粒子激光脉冲蒸馏水产量效应纳米颗粒悬浮液 YAG激光器激光烧蚀速度控制

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Whole-genome amplification/preimplantation genetic testing for propionic acidemia of successful pregnancy in an obligate carrier Mexican couple:A case report

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World Journal of Clinical Cases 2021年第29期9卷 8797-8803页

作者： Adina Neumann miguel angel Alcantara-Ortigoza Ariadna González-del angel Nestor Alejandro Zarate Díaz Javier Sam santana Leonardo M Porchia Esther López-Bayghen Laboratorio de Investigación y Diagnóstico Molecular IngenesMexico City 05320Mexico Laboratorio de Biología Molecular Subdirección de Investigación MédicaInstituto Nacional de PediatríaMexico City 04530Mexico DNA-GEN S.C.Centro de Alta Especialidad en Genética Humana Mexico City 14070Mexico Investigación Clínica IngenesPuebla 72820Mexico Departamento de Toxicología Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalMexico City 07360Mexico

BACKGROUND Identifying a potential single monogenetic disorder in healthy couples is costly due to the Assisted Reproduction facilities'current methodology for screening,which focuses on the detecting multiple genetic disorders at ***,we report the successful application of a low-cost and fast preimplantation genetic testing for monogenic/single gene defects(PGT-M)approach for detecting propionic acidemia(PA)in embryos obtained from a confirmed heterozygous propionyl-CoA carboxylase alpha subunit(PCCA)*** SUMMARY A fertile 32-years old Mexican couple with denied consanguinity sought antenatal genetic *** were suspected obligate PA carriers due to a previous deceased PA male newborn with an unknown PCCA/propionyl-CoA carboxylase beta subunit(PCCB)***-Generation Sequencing revealed a heterozygous genotype for a pathogenic PCCA variant(c.2041-1G>T,ClinVar:RCV-000802701.1;dbSNP:rs1367867218)in both *** couple requested in vitro fertilization(IVF)and PGT-M for *** IVF,12 oocytes were collected and fertilized,of which two resulted in high-quality *** biopsies and Whole Genome Amplification by a fragmentation/amplification-based method were performed and revealed that the two embryos were *** polymerase chain reaction and further Sanger sequencing of the exon-intron borders revealed a wild-type PCCA male embryo and a heterozygous c.2041-1G>T female *** embryos were transferred,resulting in a clinical pregnancy and the delivery of a healthy male newborn(38 wk,weight:4080 g,length:49 cm,APGAR 9/9).The absence of PA was confirmed by expanded newborn *** We show that using PGT-M with Whole Genome Amplification templates,coupled with IVF,can reduce the transmission of a pathogenic variant of the PCCA gene.

关键词： Propionic acidemia Autosomal recessive Propionyl-CoA carboxylase alpha subunit(PCCA)gene Preimplantation genetic testing Next-generation sequencing Embryo transfer

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Photomechanical Ablation of 304L Stainless Steel, Aluminum Oxide (Al₂O₃) Thin Film, and Pure Silicon

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Optics and Photonics Journal 2016年第10期6卷 275-288页

作者： Gabriel de la Rosa-santana Jose Alfredo Alvarez-Chavez Hector R. Morano-Okuno angel J. Morales-Ramirez Esmeralda Uribe Instituto Tecnologico y de Estudios Superiores de Monterrey Campus Estado de Mexico Atizapan de Zaragoza Mexico Instituto Politecnico Nacional-Centro de Investigacion e InnovacionTecnologica (CIITEC-IPN) Mexico City Mexico Instituto Tecnologico y de Estudios Superiores de Monterrey Campus Queretaro Santiago de Queretaro Mexico

Recently, a number of studies have focused on micro-manufacturing processes, which find use in a variety of applications, including the production of microelectromechanical systems (MEMS). The process of ablation in materials is mainly governed by the laser source and scanning speed. The rate of material ablation is influenced by chemical and physical properties. In this work, the energy from a CO2 laser was used to ablate three different materials, namely, stainless steel 304L, a thin film of amorphous aluminum oxide (Al2O3), and pure silicon, due to their wide use in MEMS technology. The laser parameters used were an average power of 18 W and a spot size of 200 μm. The maximum depth during the photomechanical ablation process was 72 μm in the case of 304L steel and 77 μm in the case of the Al2O3 thin film for a scan rate of 24 mm/min. However, at the same scan rate, silicon did not exhibit any penetration. As expected, while increasing scanning speed the ablation depth decreases due to reduced interaction time between laser and material. The theoretical ytterbium fiber laser shown in this study can thus be employed in the manufacturing of a wide variety of materials used in the production of MEMS as well as those used in clean energy technologies.

关键词： Laser Ablation MEMS Ytterbium-Doped Fiber Laser Thin Film

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