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Effects of desiccation of seeds in nine species with morphophysiological dormancy on germination and embryo growth

Journal of Plant Ecology 2021年第1期14卷 132-146页

作者： miguel a.copete Jose M.Herranz Raquel Herranz Elena.copete Pablo Ferrandis ETSIAM Department of Plant Production and Agricultural TechnologyUniversity of Castilla-La ManchaUniversity Campus s/nAlbacete 02071Spain Botanical Institute University of Castilla-La ManchaAvenida de la Mancha s/nAlbacete 02006Spain

Aims In species with morphophysiological seed dormancy(MPD),little is known about the effects of desiccation of imbibed seeds on embryo growth and *** studied seed responses to dehydration in nine species with different levels of *** For each species,a control test was conducted by keeping seeds permanently hydrated and exposed to the optimal stratification-incubation sequenee to promote embryo ***,tests were run in which seed stratification was interrupted for 1 month by desiccation at room *** Findings In Clematis vitalba and Ribes alpinum,with non deep simple MPD,desiccation affected neither embryo growth nor seed viability,but the desiccation led to a decrease of germinative ability in *** by 16%.The seeds of Narcissus pseudonarcissus ***-garmendiae,with deep simple epicotyl MPD,tolerated desiccation in different embryo growth stages,but their germinative ability decreased *** response of species with complex levels of MPD to desiccation was more variable:Delphinium fissum ***,with intermediate complex MPD,and Anthriscus sylvestris and Meum athamanticum,both with deep complex MPD,tolerated *** contrast,Ribes uva-crispa with nondeep complex MPD,Lonicera pyrenaica with intermediate complex MPD and Chaerophyllum aureum with deep complex MPD,had diminished germination ability by *** seeds of the species with simple levels of MPD tolerated desiccation,those of some species with complex levels were also highly ***,desiccation did not induce secondary dormancy in late embryo growth *** desiccation tolerance of imbibed seeds of most of the nine species may show their adaptability to climate change in the Mediterranean region.

关键词： complex morphophysiological dormancy germinative ability simple morphophysiological dormancy secondary dormancy viability

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Comparison between standard and wide-field autofluorescence in detection of retinal displacement after rhegmatogenous retinal detachment surgery

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Advances in Ophthalmology Practice and Research 2022年第3期2卷 32-37页

作者： Raquel Maroto Cejudo Cristina Blanco Marchite Teresa Prieto Moran Sergio copete Piqueras Retina Department Service of OphthalmologyAlbacete University ComplexAlbaceteSpain

Purpose To analyse the differences between 30°blue autofluorescence(BAF30),55°blue autofluorescence(BAF55)and 200°green Ultra-Wide Field autofluorescence(UWF200)imaging to detect retinal displacement(RD)after vitrectomy surgery for rhegmatogenous retinal detachment(RRD).Methods This cross-sectional study considers forty-nine consecutive patients who underwent RRD surgery in the time period 4–8 months previous to image *** exclusion criteria contemplate previous retinal pathology,under 18-year-olds and non-assessable images in any of the 3 *** images were analysed by two masked graders that assessed either the presence or absence of retinal displacement.A third observer reviewed the images that presented *** A total of forty-nine patients were analysed.7 eyes were excluded due to poor quality in either of the imaging *** final analysis included 42 eyes of forty-two patients with a mean age of 60.3±11.9 *** patients underwent a 3 port 23-gauge pars plana vitrectomy as the technique of *** grade of RD was detectable in 45.2% of *** was similar between BAF30 and UWF200(42.9% of eyes).BAF55 showed RD to a lesser extent(38.1%).Agreement index between BAF30 and BAF55 was 0.901,0.903 between BAF30 and UWF200 and 0.803 between BAF55 and *** agreement index between graders was 0.775 for BAF30,0.798 for BAF50 and 0.808 for UWF200 *** All imaging modalities were able to detect RD after vitrectomy for RRD,with no inferiority of BAF30 and BAF55 over UWF200.

关键词： Blue fundus autofluorescence Green fundus autofluorescence Rhegmatogenous retinal detachment Retinal displacement Retinal vessel printings

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