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Perception of Disasters and Land Reclamation in an Informal Settlement on Reclaimed Land:Case of the BASECO Compound,Manila,the Philippines

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International Journal of Disaster Risk Science 2020年第5期11卷 640-654页

作者： Ven Paolo Bruno Valenzuela miguel esteban Onuki Motoharu Graduate Program in Sustainability Science-Global Leadership Initiative The University of TokyoKashiwaChiba 277-8563Japan Department of Civil and Environmental Engineering Waseda UniversityTokyo 169-8555Japan

Southeast Asia's coastal urban areas continue to grow,with land reclamation fast becoming an important option for megacities to address issues of economic growth and increasing population *** are divided over the advantages and disadvantages of land reclamation,though this process continues unabated,exposing settlements to coastal *** Bataan Shipping and Engineering Company(BASECO)compound is an informal settlement on reclaimed land in Manila,the *** informal coastal settlements view disaster risk and their more pressing socioeconomic needs is crucial to understanding the potentials and repercussions of land *** a topographic survey,a questionnaire survey,and the protective action decision model,this study explored the perception of disasters and land reclamation of informal settlers who are living on reclaimed *** study found that people are aware of disaster risks,but are more concerned with everyday *** are divided on the issue of further land *** on the original non-reclaimed land view it as a coastal defense,while those on reclaimed land fear potential *** this,all locations in the community are concerned with the loss of jobs,economic opportunities,and eviction,rather than potential disasters.

关键词： Coastal hazards Flooding Informal settlements Land reclamation Philippines Risk perception Urbanization

Back to pluripotency:fully chemically induced reboot of human somatic cells

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Signal Transduction and Targeted Therapy 2022年第8期7卷 2658-2659页

作者： Lucas Lange miguel A.esteban Axel Schambach Institute of Experimental Hematology Hannover Medical SchoolHannover 30625Germany REBIRTH Research Center for Translational Regenerative Medicine Hannover Medical SchoolHannover 30625Germany Laboratory of Integrative Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Division of Hematology/Oncology Boston Children’s HospitalHarvard Medical SchoolBostonMA 02115USA

In a recent study published in Nature,Guan et al.1 showed an exciting strategy to reprogram human somatic cells into human chemically induced pluripotent stem cells(hCiPSCs).Thereby,they identified crucial steps and p... 详细信息

关键词： al. chemically steps

Role of Long Non-coding RNAs in Reprogramming to Induced Pluripotency

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Genomics, Proteomics & Bioinformatics 2020年第1期18卷 16-25页

作者： Shahzina Kanwal Xiangpeng Guo Carl Ward Giacomo Volpe Baoming Qin miguel A.esteban Xichen Bao Joint School of Life Sciences Guangzhou Medical University and Guangzhou Institutes of Biomedicine and HealthGuangzhou 511436China Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Laboratory of RNA Chromatinand Human DiseaseGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Guangzhou Regenerative Medicine and Health Guangdong Laboratory(GRMH-GDL) Guangzhou 510005China Laboratory of Metabolism and Cell Fate Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Institute for Stem Cells and Regeneration Chinese Academy of SciencesBeijing 100101China Laboratory of RNA Molecular Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China

The generation of induced pluripotent stem cells through somatic cell reprogramming requires a global reorganization of cellular *** reorganization occurs in a multi-phased manner and involves a gradual revision of both the epigenome and *** studies have shown that the large-scale transcriptional changes observed during reprogramming also apply to long noncoding RNAs(lncR NAs),a type of traditionally neglected RNA species that are increasingly viewed as critical regulators of cellular *** understanding of lncR NAs in reprogramming may not only help to improve this process but also have implications for studying cell plasticity in other contexts,such as development,aging,and *** this review,we summarize the current progress made in profiling and analyzing the role of lncR NAs in various phases of somatic cell reprogramming,with emphasis on the re-establishment of the pluripotency gene network and X chromosome reactivation.

关键词： Mesenchymal-to-epithelial transition X chromosome reactivation Apoptosis Proliferation

Towards a primate single-cell atlas

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Zoological Research 2022年第4期43卷 691-694页

作者： Xiao Zhang Guang-Yao Lai Giacomo Volpe Lei Han Patrick H.Maxwell Long-Qi Liu miguel A.esteban Yi-Wei Lai Jilin Provincial Key Laboratory of Animal Embryo Engineering Institute of ZoonosisCollege of Veterinary MedicineJilin UniversityChangchunJilin 130062China Joint School of Life Sciences Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical UniversityGuangzhouGuangdong 511436China Hematology and Cell Therapy Unit IRCCS-Istituto Tumori‘Giovanni Paolo II’Bari 70124Italy BGI-Shenzhen ShenzhenGuangdong 518103China Cambridge Institute for Medical Research Department of MedicineUniversity of CambridgeCambridge CB20XYUK Laboratory of Integrative Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhouGuangdong 510530China

Biology in the 21st century is shifting substantially towards single-cell *** tissues are highly heterogeneous and contain multiple cell types in different states whose tight coordination determines overall *** measurements,including RNA sequencing,provide average values,which may dilute cell-specific effects or overlook rare effects of functional *** greatly limits our understanding of mammalian physiology and *** example,the histological structures of the cortical and medullary regions of the kidney differ substantially,and their cells exhibit distinct characteristics related to environmental gradients such as oxygenation,glomerular filtrate-induced shear stress,and solute concentrations within the ***,not all nephrons,mesenchymal cells,or vascular cells function in the same way,especially in the context of disease and aging.

关键词： analysis histological shifting

Intricacies in the cross talk between metabolic enzymes,RNA,and protein translation

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Journal of Molecular Cell Biology 2019年第10期11卷 813-815页

作者： Yuan Lv Muqddas Tariq Xiangpeng Guo Shahzina Kanwal miguel Aesteban Joint School of Life Sciences Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical UniversityGuangzhou 511436China Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Laboratory of RNA Chromatinand Human DiseaseGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Guangzhou Regenerative Medicine and Health Guangdong Laboratory Guangzhou 510005China University of Chinese Academy of Sciences Beijing 100049China Institute for Stem Cells and Regeneration Chinese Academy of SciencesBeijing 100101China

The development of tech niq ues allowing the systematic capture of the RNA-bound proteome has yielded many *** these,metabolic enzymes have been frequently detected as RNA-binding proteins(RBPs)by different profiling methodologies in various cell types(Hentze et al.,2018).Compared to previous-more simplistic-views,it is now known that cellular metabolism(not only limited to the tricarboxylic acid,TCA,cycle)is *** fact,metabolic enzymes translocate to the nucleus and are enriched at actively transcribed loci,where they sustain epigenetic/epitranscriptomic marks through localized metabolite production(Mews et al.,2017).It has been proposed that the in teraction with nascent RNAs facilitates the anchorage of metabolic enzymes at target loci(Bao et al.,2018),and it is plausible that mature RNAs work in the same way to assist metabolic reactions in the cytoplasm.

关键词： al. metabolism metabolic

Global Profiling of the Lysine Crotonylome in Different Pluripotent States

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Genomics, Proteomics & Bioinformatics 2021年第1期19卷 80-93页

作者： Yuan Lv Chen Bu Jin Meng Carl Ward Giacomo Volpe Jieyi Hu Mengling Jiang Lin Guo Jiekai Chen miguel A.esteban Xichen Bao Zhongyi Cheng Laboratory of Integrative Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China Jingjie PTM BioLab(Hangzhou)Co.Ltd Hangzhou 310018China Joint School of Life Sciences Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical UniversityGuangzhou 511436China Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Guangzhou 510005China Institute of Stem Cells and Regeneration Chinese Academy of SciencesBeijing 100101China Laboratory of RNA Molecular Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China

Pluripotent stem cells(PSCs)can be expanded in vitro in different culture conditions,resulting in a spectrum of cell states with distinct *** how PSCs transition from one state to another,ultimately leading to lineage-specific differentiation,is important for developmental biology and regenerative *** there is significant information regarding gene expression changes controlling these transitions,less is known about post-translational modifications of *** crotonylation is a newly discovered post-translational modification where lysine residues are modified with a crotonyl ***,we employed affinity purification of crotonylated(LC–MS/MS)to systematically profile protein crotonylation in mouse PSCs in different states including ground,metastable,and primed states,as well as metastable PSCs undergoing early pluripotency *** successfully identified 3628 high-confidence crotonylated sites in 1426 *** crotonylated proteins are enriched for factors involved in functions/processes related to pluripotency such as RNA biogenesis,central carbon metabolism,and proteasome ***,we found that increasing the cellular levels of crotonyl-coenzyme A(crotonyl-CoA)through crotonic acid treatment promotes proteasome activity in metastable PSCs and delays their differentiation,consistent with previous observations showing that enhanced proteasome activity helps to sustain *** atlas of protein crotonylation will be valuable for further studies of pluripotency regulation and may also provide insights into the role of metabolism in other cell fate transitions.

关键词： Metabolism Crotonylation Pluripotency RNA-binding proteins Proteasome

The propensity for tumorigenesis in human induced pluripotent stem cells is related with genomic instability

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Chinese Journal of Cancer 2013年第4期32卷 205-212页

作者： Yi Liang Hui Zhang Qi-Sheng Feng Man-Bo Cai Wen Deng Dajiang Qin Jing-Ping Yun George Sai Wah Tsao Tiebang Kang miguel Angel esteban Duanqing Pei Yi-Xin Zeng State Key Laboratory of Oncology in South China Department of Experimental Research Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou Department of Anatomy The University of Hong Kong Department of Pathology Sun Yat-sen University Cancer Center

The discovery of induced pluripotent stem cells (iPSCs) is a promising advancement in the field of regenerative medicine. Previous studies have indicated that the teratoma-forming propensity of iPSCs is variable; however, the relationship between tumorigenic potential and genomic instability in human iPSCs (HiPSCs) remains to be fully elucidated. Here, we evaluated the malignant potential of HiPSCs by using both colony formation assays and tumorigenicity tests. We demonstrated that HiPSCs formed tumorigenic colonies when grown in cancer cell culture medium and produced malignancies in immunodeficient mice. Furthermore, we analyzed genomic instability in HiPSCs using whole-genome copy number variation analysis and determined that the extent of genomic instability was related with both the cells′ propensity to form colonies and their potential for tumorigenesis. These findings indicate a risk for potential malignancy of HiPSCs derived from genomic instability and suggest that quality control tests, including comprehensive tumorigenicity assays and genomic integrity validation, should be rigorously executed before the clinical application of HiPSCs. In addition, HiPSCs should be generated through the use of combined factors or other approaches that decrease the likelihood of genomic instability.

关键词： Autophagy, fusion oncoprotein, acute myeloid leukemia

Generation of knockout rabbits using transcription activator-like effector nucleases

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Cell Regeneration 2014年第1期3卷 21-29页

作者： Yu Wang Nana Fan Jun Song Juan Zhong Xiaogang Guo Weihua Tian Quanjun Zhang Fenggong Cui Li Li Philip N Newsome Jon Frampton miguel a esteban Liangxue Lai Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthGuangzhou 510530China NIHR Liver BRU and Centre for Liver Research University of BirminghamBirminghamUK Liver and Hepatobiliary Unit Queen Elizabeth Hospital BirminghamBirminghamUK Institute for Biomedical Research College of Medical and Dental SciencesUniversity of BirminghamBirmingham B152TTUK

Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platformscontributing to redefine the boundaries of modern biological research. They are composed of a non-specificcleavage domain and a tailor made DNA-binding module, which enables a broad range of genetic modifications byinducing efficient DNA double-strand breaks at desired loci. Among other remarkable uses, these nucleases havebeen employed to produce gene knockouts in mid-size and large animals, such as rabbits and pigs, *** approach is cost effective, relatively quick, and can produce invaluable models for human disease studies,biotechnology or agricultural purposes. Here we describe a protocol for the efficient generation of knockout rabbitsusing transcription activator-like effector nucleases, and a perspective of the field.

关键词： Rabbits Animal models Zinc-finger nucleases Transcription activator-like effector nucleases TALENs Genome editing Knockout

Assessment of compressive strength of jet grouting by machine learning

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第1期16卷 102-111页

作者： esteban Diaz Edgar Leonardo Salamanca-Medina Roberto Tomas Departamento de Ingeniería Civil Escuela Politécnica SuperiorUniversidad de AlicanteP.O.Box 99E-03080AlicanteSpain

Jet grouting is one of the most popular soil improvement techniques,but its design usually involves great uncertainties that can lead to economic cost overruns in construction *** high dispersion in the properties of the improved material leads to designers assuming a conservative,arbitrary and unjustified strength,which is even sometimes subjected to the results of the test *** present paper presents an approach for prediction of the uniaxial compressive strength(UCS)of jet grouting columns based on the analysis of several machine learning algorithms on a database of 854 results mainly collected from different research *** selected machine learning model(extremely randomized trees)relates the soil type and various parameters of the technique to the value of the compressive *** the complex mechanism that surrounds the jet grouting process,evidenced by the high dispersion and low correlation of the variables studied,the trained model allows to optimally predict the values of compressive strength with a significant improvement with respect to the existing ***,this work proposes for the first time a reliable and easily applicable approach for estimation of the compressive strength of jet grouting columns.

关键词： Jet grouting Ground improvement Compressive strength Machine learning