检索结果-南通市图书馆

Computed tomography and photoacoustic imaging guided photodynamic therapy against breast cancer based on mesoporous platinum with insitu oxygen generation ability

引用

Acta Pharmaceutica Sinica B 2020年第9期10卷 1719-1729页

作者： lingyan Zhang mifang li Quan Zhou Meng Dang Yuxia Tang Shouju Wang Jijun Fu Zhaogang Teng Guangming Lu Department of Medical Imaging Jinling HospitalThe First School of Clinical MedicineSouthern Medical UniversityNanjing 210002China Department of Medical Imaging The Third Affiliated HospitalSouthern Medical UniversityGuangzhou 510630China Key Laboratory for Organic Electronics&Information Displays and Institute of Advanced Materials Jiangsu Key Laboratory for BiosensorsJiangsu National Synergetic Innovation Centre for Advanced MaterialsNanjing University of Posts and TelecommunicationsNanjing 210046China Departm ent of Medical Imaging Jiangsu Province HospitalNanjing 210029China Key Laboratory of Molecular Target&Clinical Pharmacology School of Pharmaceutical SciencesGuangzhou Medical UniversityGuangzhou 511436China

Photodynamic therapy(PDT)has been widely used in cancer ***,hypoxia in most solid tumors seriously restricts the efficacy of *** improve the hypoxic microenvironment,we designed a novel mesoporous platinum(mPt)nanoplatform to catalyze hydrogen peroxide(H2 O2)within the tumor cells in situ without an extra *** the fabrication,the carboxy terminus of the photosensitizer chlorin e6(Ce6)was connected to the amino terminus of the bifunctional mercaptoaminopolyglycol(SH-PEG-NH2)by a condensation reaction,and then PEG-Ce6 was modified onto the mPt moiety via the mercapto terminal of ***,cellular and animal experiments demonstrated that Pt@PEG-Ce6 catalyzed H2 O2 to produce oxygen(O2)and that Ce6 transformed O2 to generate reactive oxygen species(ROS)upon laser *** Pt@PEG-Ce6 nanoplatform with uniform diameter presented good biocompatibility and efficient tumor *** to the high atomic number and good near-infrared absorption for Pt,this Pt@PEG-Ce6 nanoplatform showed computed tomography(CT)and photoacoustic(PA)dual-mode imaging ability,thus providing an important tool for monitoring the tumor hypoxic ***,the Pt@PEG-Ce6 nanoplatform reduced the expression of hypoxia-inducible factor-la(HIF-1α)and programmed death-1(PD-1)in tumors,discussing the relationship between hypoxia,PD-1,and PDT for the first time.

关键词： Mesoporous platinum Photodynamic therapy Tumor hypoxia Photoacoustic imaging Computed tomography imaging

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

术前MRI测量腘绳肌肌腱横截面积预测前交叉韧带重建术中移植物直径的初步研究

引用

中华放射学杂志 2023年第3期57卷 288-293页

作者：张妃媛李米芳林秋雨管雪琴张灵艳周全南方医科大学第三附属医院医学影像科广州510630 深圳市龙岗中心医院医学影像科深圳518116

目的探讨术前MRI测量的腘绳肌(半腱肌+股薄肌)肌腱横截面积预测前交叉韧带重建(ACLR)术中移植物直径的价值。方法回顾性收集2013年1月至2020年8月在南方医科大学第三附属医院进行4股腘绳肌肌腱单束ACLR患者的术前膝关节MRI图像, 共265... 详细信息

目的探讨术前MRI测量的腘绳肌(半腱肌+股薄肌)肌腱横截面积预测前交叉韧带重建(ACLR)术中移植物直径的价值。方法回顾性收集2013年1月至2020年8月在南方医科大学第三附属医院进行4股腘绳肌肌腱单束ACLR患者的术前膝关节MRI图像, 共265例。根据术中移植物直径将患者分为移植物直径≥8 mm组(129例)和移植物直径<8 mm组(136例)。在术前MRI横断面图像股骨内外髁最大层面测量半腱肌和股薄肌肌腱的横截面积, 将两者相加获得腘绳肌肌腱横截面积。使用独立样本t检验比较移植物≥8 mm组与移植物<8 mm组间各肌腱横截面积的差异, 采用Spearman相关分析评估肌腱横截面积与术中移植物直径的相关性, 使用多因素logistic回归分析筛选肌腱横截面积中对术中移植物直径的影响因素, 绘制受试者操作特征曲线评估其预测术中移植物直径≥8 mm的效能。结果术中测量移植物直径为7.5(7.5, 8.0)mm。移植物直径≥8 mm组腘绳肌肌腱横截面积为(21.4±4.6)mm^(2), 移植物直径<8 mm组腘绳肌肌腱横截面积为(15.6±3.7)mm^(2)。移植物直径≥8 mm组和移植物直径<8 mm组间半腱肌、股薄肌、腘绳肌肌腱横截面积差异均有统计学意义(t=-10.26、-10.29、-11.47, P<0.001)。术中移植物直径与半腱肌、股薄肌、腘绳肌横截面积呈正相关, 相关系数分别为0.57(P<0.001)、0.58(P<0.001)、0.62(P<0.001)。多因素logistic回归结果显示腘绳肌肌腱横截面积是预测术中移植物直径的影响因素(OR=1.45, 95%CI 1.32~1.59, P<0.001)。腘绳肌肌腱横截面积预测术中移植物直径≥8 mm的曲线下面积为0.838(95%CI 0.792~0.885), 临界值为20.0 mm^(2), 灵敏度为0.581, 特异度为0.941。结论术前MRI测量腘绳肌肌腱横截面积可用于预测自体腘绳肌移植物直径大小。

关键词：前交叉韧带重建腘绳肌肌腱磁共振成像移植物直径

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

定量T2 mapping评估前交叉韧带重建术后6个月、12个月移植物成熟度变化并与临床评分相关性

引用

临床放射学杂志 2024年第1期43卷 110-115页

作者：张妃媛管雪琴李米芳林秋雨陈燚林张灵艳周全深圳市龙岗中心医院医学影像科 518116 南方医科大学第三附属医院医学影像科广州510630

目的利用T2 mapping功能磁共振定量检测前交叉韧带重建术(ACLR)后6个月、12个月移植物重塑状态并与临床评分相关性。方法前瞻性搜集行ACLR的患者40例,ACLR后患者在6个月、12个月都接受3.0 T常规MRI及T2 mapping扫描,测得术后移植物关节... 详细信息

目的利用T2 mapping功能磁共振定量检测前交叉韧带重建术(ACLR)后6个月、12个月移植物重塑状态并与临床评分相关性。方法前瞻性搜集行ACLR的患者40例,ACLR后患者在6个月、12个月都接受3.0 T常规MRI及T2 mapping扫描,测得术后移植物关节内段整体及其三个分段(近、中、远)T2值。术后6个月、12个月使用国际膝关节文献委员会膝关节评分(IKDC)进行临床功能评分。使用独立样本t检验用于分析术后6个月、12个月的关节三段以及关节内段整体T2值之间的差异。关节内段三段采用LSD-t法进行两两比较。采用Spearman相关分析评估移植物关节内段T2值与临床评分相关性。结果前交叉韧带移植物整体关节内段及其三个分段(近、中、远)的T2值在6个月到12个月内明显下降(P值均<0.001)。术后6个月关节内段的近段的T2值较中段及远段高(P=0.026和P=0.001),术后12个月,关节内段的近段的T2值较远段高(P<0.001)。术后6个月移植物T2值与IKDC评分呈中度负相关(r=-0.508,P=0.001),术后12个月移植物T2值与IKDC评分呈中度负相关(r=-0.559,P<0.001)。结论T2 mapping可以检测ACLR术后6个月到12个月移植物关节内段成熟度变化,具有潜在临床应用价值。

关键词：前交叉韧带重建术移植物成熟 T2 mapping

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

应用高通量测序技术鉴别中国输入性黄热病毒野毒株与疫苗株

引用

中华实验和临床病毒学杂志 2017年第4期31卷 353-356页

作者：刘林张益李阿茜张硕张全福李川严延生马学军梁米芳李德新中国疾病预防控制中心病毒病预防控制所北京102206 福建省疾病预防控制中心福州350001

目的鉴别2016年3月我国3例输入性黄热病例为2016年安哥拉流行的黄热病毒野毒株感染,还是黄热病毒疫苗相关疾病,亦或是二者的混合感染.方法应用IonTorrent PGM测序平台,对3例黄热病病例血液或尿液标本进行高通量测序,对所获得的3株黄... 详细信息

目的鉴别2016年3月我国3例输入性黄热病例为2016年安哥拉流行的黄热病毒野毒株感染,还是黄热病毒疫苗相关疾病,亦或是二者的混合感染.方法应用IonTorrent PGM测序平台,对3例黄热病病例血液或尿液标本进行高通量测序,对所获得的3株黄热病毒序列进行基因组特征分析.分析黄热病毒野毒株与疫苗株核酸序列差异,以同源性较低区域序列作为参考序列将高通量测序获得的短序列做回贴和比对.结果从3份黄热患者标本中获得了部分黄热病毒基因组,其中从第二例患者标本中获得了全长蛋白编码区序列.3株病毒与2016年从中国第一例输入性黄热病例中分离的病毒株CNYF01 R/2016株和来自安哥拉的1971年毒株Angola71株具有极高的相似性,都属于安哥拉型黄热病毒.分析得到5段黄热病毒野毒株与疫苗株核酸序列差异较大的区域,在这5段区域中,3份标本测序获得短序列与野毒株均有多个匹配,而与疫苗株则没有匹配.结论 3例黄热患者的血液或尿液标本中未检测到黄热病毒疫苗株17D株基因组序列,高通量测序证实3人均为2016年安哥拉流行的黄热病毒野毒株感染.

关键词：黄热病毒高通量测序

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

NERC-300高频焊接仪在大鼠肝部分切除术中的对比研究

引用

重庆医学 2018年第5期47卷 601-603页

作者：潘东岳蔡寨韩帅厉周申梦莹周栩悦周嘉莹李米芳南方医科大学珠江医院普通外科广州510282

目的探讨NERC-300高频焊接仪与超声刀在大鼠肝部分切除术中的效果。方法将48只大鼠分为实验组和对照组各24只。实验组应用NERC-300高频焊接仪行肝部分切除术,对照组采用超声刀,比较两组大鼠存活率、手术时间、出血量、工作温度、热损伤... 详细信息

目的探讨NERC-300高频焊接仪与超声刀在大鼠肝部分切除术中的效果。方法将48只大鼠分为实验组和对照组各24只。实验组应用NERC-300高频焊接仪行肝部分切除术,对照组采用超声刀,比较两组大鼠存活率、手术时间、出血量、工作温度、热损伤范围及术后第1、3、7天肝功能变化。结果术后第3天肝功能实验组恢复优于对照组(P0.05)。结论 NERC-300高频焊接仪行大鼠肝部分切除安全且有效。

关键词：高频焊接肝部分切除大鼠,Wistar 超声刀

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SNX11 Identified as an Essential Host Factor for SFTS Virus Infection by CRISPR Knockout Screening

引用

Virologica Sinica 2019年第5期34卷 508-520页

作者： Tiezhu liu Jiajia li Yang liu Yuanyuan Qu Aqian li Chuan li Quanfu Zhang Wei Wu Jiandong li Yan liu Dexin li Shiwen Wang mifang liang Key Laboratory of Medical Virology and Viral Diseases Ministry of Health of People's Republic of ChinaNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China CDC-WIV Joint Research Center for Emerging Diseases and Biosafety Wuhan 430071China Department of Microbiology Anhui Medical UniversityHefei 230032China The First Affiliated Hospital of Anhui Medical University Hefei 230022China

Severe fever with thrombocytopenia syndrome virus(SFTSV)is a highly pathogenic tick-borne bunyavirus that causes lethal infectious disease and severe fever with thrombocytopenia syndrome(SFTS)in *** molecular mechanisms and host cellular factors required for SFTSV infection remain *** a genome-wide CRISPR-based screening strategy,we identified a host cellular protein,sorting nexin 11(SNX11)which is involved in the intracellular endosomal trafficking pathway,as an essential cell factor for SFTSV *** SNX11-KO HeLa cell line was established,and SFTSV replication was significantly *** glycoproteins of SFTSV were detected and remained in later endosomal compartments but were not detectable in the endoplasmic reticulum(ER)or Golgi *** values in the endosomal compartments of the SNX11-KO cells increased compared with the pH of normal HeLa cells,and lysosomal-associated membrane protein 1(LAMP1)expression was significantly elevated in the SNX11-KO ***,these results indicated that penetration of SFTSV from the endolysosomes into the cytoplasm of host cells was blocked in the cells lacking *** study for the first time provides insight into the important role of the SNX11 as an essential host factor in the intracellular trafficking and penetrating process of SFTSV infection via potential regulation of viral protein sorting,membrane fusion,and other endocytic machinery.

关键词： CRISPR screen Severe fever with thrombocytopenia syndrome virus(SFTSV) Host factor Sorting nexin 11(SNX11)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development of a reverse transcription quantitative polymerase chain reaction-based assay for broad coverage detection of African and Asian Zika virus lineages

引用

Virologica Sinica 2017年第3期32卷 199-206页

作者： Yang Yang Gary Wong Baoguo Ye Shihua li Shanqin li Haixia Zheng Qiang Wang mifang liang George F Gao Lei liu Yingxia liu Yuhai Bi Shenzhen Key Laboratory of Pathogen and Immunity State Key Discipline of Infectious DiseaseShenzhen Third People’s Hospital CAS Key Laboratory of Pathogenic Microbiology and Immunology Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease Institute of Microbiology Center for Influenza Research and Early-warning (CASCIRE) Chinese Academy of Sciences China-Japan Union Hospital of Jilin University Key Laboratory for Medical Virology NHFPC National Institute for Viral Disease Control and Prevention China CDC Office of Director-General Chinese Center for Disease Control and Prevention Savaid Medical School University of Chinese Academy of Sciences

The Zika virus(ZIKV) is an arbovirus that has spread rapidly worldwide within recent times. There is accumulating evidence that associates ZIKV infections with Guillain-Barré Syndrome(GBS) and microcephaly in humans. The ZIKV is genetically diverse and can be separated into Asian and African lineages. A rapid, sensitive, and specific assay is needed for the detection of ZIKV across various pandemic regions. So far, the available primers and probes do not cover the genetic diversity and geographic distribution of all ZIKV strains. To this end, we have developed a one-step quantitative reverse transcription polymerase chain reaction(qRT-PCR) assay based on conserved sequences in the ZIKV envelope(E) gene. The detection limit of the assay was determined to be five RNA transcript copies and 2.94 × 10^(–3) 50% tissue culture infectious doses(TCID50) of live ZIKV per reaction. The assay was highly specific and able to detect five different ZIKV strains covering the Asian and African lineages without nonspecific amplification, when tested against other flaviviruses. The assay was also successful in testing for ZIKV in clinical samples. Our assay represents an improvement over the current methods available for the detection ZIKV and would be valuable as a diagnostic tool in various pandemic regions.

关键词： Flavivirus Zika virus(ZIKV) molecular diagnostics qRT-PCR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Investigation of Nasal Mucosal IgA Responses in the Population Following COVID-19 Pandemic — China, September 2022–August 2023

引用

China CDC weekly 2024年第15期6卷 312-317,I0005页

作者： Boyang li Lei Zhou Zhilong Chen Yanhan Wen Qian Wang Tao Huang Si Chen Wei Wu Mengxuan Wang lina Sun mifang liang Shiwen Wang ling Chen Qun li National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionBeijingChina Xiamen United Institute of Respiratory Health Xiamen CityFujian ProvinceChina Guangzhou Laboratory Guangzhou CityGuangdong ProvinceChina State Key Laboratory of Respiratory Disease Guangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou CityGuangdong ProvinceChina

What is already known about this topic?Mucosal IgA plays a crucial role in host immunity against respiratory *** studies suggest that it has the potential to mitigate the transmission of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron ***,a comprehensive population-based analysis examining mucosal IgA levels following the winter 2022 wave of the coronavirus disease 2019(COVID-19)pandemic is yet to be *** is added by this report?In our study involving 3,421 participants,we documented IgA responses subsequent to SARS-CoV-2 infection.A significant proportion of individuals sustained increased levels of IgA for over six *** levels were also observed in individuals with prior infections who underwent asymptomatic reinfections,indicating an active production of IgA ***,individuals with multiple vaccinations or severe symptoms tended to display elevated IgA levels after *** are the implications for public health practice?IgA in the nasal mucosa is crucial for defense against SARS-CoV-2 *** insights can enhance our knowledge of immune responses following infection and have provided certain reference values for disease prevention and control strategies.

关键词： prevention sustained immunity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rift Valley Fever Virus and Yellow Fever Virus in Urine: A Potential Source of Infection

引用

Virologica Sinica 2019年第3期34卷 342-345页

作者： Meng li Beibei Wang liqiang li Gary Wong Yingxia liu Jinmin Ma Jiandong li Hongzhou Lu mifang liang Ang li Xiuqing Zhang Yuhai Bi Hui Zeng BGI Education Center University of Chinese Academy of SciencesShenzhen 518083China Beijing Key Laboratory of Emerging Infectious Diseases Institute of Infectious DiseasesBeijing Ditan HospitalCapital Medical UniversityBeijing 100015China China National GeneBank BGI-ShenzhenShenzhen 518083China Shenzhen Key Laboratory of Pathogen and Immunity Guangdong Key Laboratory for Diagnosis and Treatment of Emerging Infectious DiseasesState Key Discipline of Infectious DiseaseSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen Third People's HospitalShenzhen 518112China Institute Pasteur of Shanghai Chinese Academy of SciencesShanghai 200031China Département de Microbiologie-Infectiologie et d'immunologie Université LavalQuébec G1V 0A6Canada Department of Internal Medicine Shanghai Medical CollegeFudan UniversityShanghai 200032China Key Laboratory of Medical Virology and Viral Diseases Ministry of HealthNational Institute for Viral Disease Control and Prevention (IVDC)Chinese Center for Disease Control and Prevention(China CDC)Beijing 102206China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyCenter for Influenza Research and Early-warning (CASCIRE)Chinese Academy of SciencesBeijing 100101China

Dear Editor,In recent years,the incidence of human infections caused by emerging or re-emerging pathogens has rapidly *** that were once regional now have the ability to spread globally in a short amount of time and pose a wider threat to public health(Weaver et al.2018).Yellow fever virus(YFV,family Flaviviridae,genus Flavivirus)is a mosquito-borne flavivirus that causes yellow fever in humans and has been endemic in Africa and Latin America for many years(Domingo et al.2018).

关键词： Yellow Fever Virus A Potential Source Infection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hemorrhagic Fever with Renal Syndrome — liaoning Province, China, 1999−2018

引用

China CDC weekly 2020年第20期2卷 350-354页

作者： Cui Shang Yingwei Sun Qiangling Yin Xiaoxia Huang Xuesheng liu Quanfu Zhang lingling Mao Chuan li Aqian li Qin Wang lina Sun mifang liang Shiwen Wang Dexin li Jiandong li Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina Institute for prevention and control of infection and infectious diseases Center for Disease Control and Prevention of Liaoning ProvinceShenyangLiaoningChina

Summary What is already known on this topic?Hemorrhagic fever with renal syndrome(HFRS)is endemic in liaoning *** Seoul and Hantaan virus are circulating in rodents,and epidemic outbreaks and sporadic cases have been ... 详细信息

关键词： Province. liaoning Hemorrhagic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：