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BmApontic is involved in neurodevelopment in the silkworm Bombyx mori

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Journal of Integrative Agriculture 2020年第6期19卷 1439-1446页

作者： MENG Miao YU Qi wang Qin LIU chun LIU Zhao-yang REN chun-jiu CUI Wei-zheng LIU Qing-xin Laboratory of Developmental Genetics Shandong Agricultural UniversityTai'an 271018P.R.China Shandong Agricultural University Library Tai'an 271018P.R.China State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqing 400715P.R.China

The nervous system of the silkworm is vital for the development of *** achieves and maintains normal life activities by regulating the function of the organs and all kinds of physiological processes in the ***(BmApt),as an imports nt bZIP tran scripti on factor,is required for the formatio n of pigme ntation in the ***,the fun ction of BmApt in the development of the nervous system of the silkworm remains ***,we showed that amino acid seque nee of BmApt was evoluti on arily con served in its Myb/SANT motif and basic DNA bindi ng *** was expressed in the nervous system at the embry onic *** n of Bmapt by RNA interfere nee resulted in abno rmal development of ***,the expression of BmnetrinA,BmnetrinB and Bmfrazzled was decreased in the Bmapt knockdown *** results dem on strate that BmApt controls neurodevelopme nt by activati ng the expressi on of Bmnetrin and Bmfrazzled.

关键词： Bombyx mori BmAp on tic Bmnetrin Bmfrazzled n eurodevelopme nt RNA interfere nee

Effect of Screw-Dislocation on Electrical Properties of Spiral-Type Bi2Se3 Nanoplates

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Chinese Journal of Chemical Physics 2016年第6期29卷 687-692,I0001页

作者： Yu-kun Wu A-wei Zhuang chun-miao Ye Jie Zeng Nan Pan Xiao-ping wang HefeiNationalLaboratoryforPhysicalSciencesattheMicroscaleandDepartmentofPhysics UniversityofScienceandTechnologyofChinaHefei230025China

We systematically investigated the electrical nanoplates through field effect transistor and properties of spiral-type and smooth Bi2Se3 conductive atomic force microscopy （CAFM） measurement. It is observed that both nanoplates possess high conductivity and show metallic-like behavior. Compared to the smooth nanoplate, the spiral-type one exhibits the higher carrier concentration and lower mobility. CAFM characterization reveals that the conductance at the screw-dislocation edge is even higher than that on the terrace, implying that the dislocation can supply excess carriers to compensate the low mobility and achieve high conductivity. The unique structure and electrical properties make the spiral-type Bi2 Se3 nanoplates a good candidate for catalysts and gas sensors.

关键词： Bi2Se3 nanoplates Screw-dislocation Electrical properties Field effect tran-sistor Conductive atomic force microscopy

Forest characteristics and population structure of Glyptostrobus pensilis, a globally endangered relict species of southeastern China

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Plant Diversity 2019年第4期41卷 237-249页

作者： Cindy Q. Tang Yongchuan Yang Arata Momohara Huan-Chong wang Hong Truong Luu Shuaifeng Li Kun Song Shenhua Qian Ben LePage Yi-Fei Dong Peng-Bin Han Masahiko Ohsawa Buu Thach Le Huu Dang tran Minh Tri Dang Ming-chun Peng Chong-Yun wang Institute of Ecology and Geobotany School of Ecology and Environmental ScienceYunnan UniversityDongwaihuan South RoadUniversity TownChenggong New DistrictKunmingYunnan 650504China The Nature Conservancy Society of Japan Mitoyo Bldg.2F1-16-10 ShinkawaChuo-kuTokyo 104-0033Japan Key Laboratory of Three Gorges Reservoir Region's Eco-Environment Ministry of EducationChongqing UniversityChongqing 400045China Graduate School of Horticulture Chiba University648 MatsudoChiba 271-8510Japan Institute of Botany Yunnan UniversityKunmingYunnan 650091China Southern Institute of Ecology Vietnam Academy o f Science and TechnologyHo Chi Minh CityViet Nam Research Institute of Resource Insects Chinese Academy of ForestryKunmingYunnan 650224China School o f Ecological and Environmental Sciences East China Normal UniversityShanghai 200241China Pacific Gas and Electric Company 3401 Crow Canyon RoadSan RamonCA 94583USA The Academy of Natural Science 1900 Benjamin Franklin ParkwayPhiladelphiaPA 19W3USA

The Chinese water pine Glyptostrobus pensilis is the sole surviving species of the genus Glyptostrobus. It is endemic to southern China, central Vietnam, and eastern Laos, and today it is nearly extinct in the wild. Forest community characteristics and population structure of G. pensilis in China have remained un-known up to now. We investigated six swamp forest stands and analyzed their forest community characteristics (i.e. vertical stratification, species composition, and diversity) and population structure, including the frequency distribution of DBH (diameter at breast height) and age-classes as found in Fujian Province, southeastern China. The vertical stratifications of all the forest stands were rather simple. The remaining wild specimens ranged from roughly 15 to some 357 years for an average of ca. 85 years, with only a few individuals less than 20 years old. Compared with the stands and populations of G. pensilis in Vietnam, the taxonomic compositions of the stands in the two regions were different, except for the dominant species-G. pensilis. The Shannon-Wiener index showed the overstory of each stand had much lower diversity (0.26 on average) in Fujian Province than that (1.97 on average) in Vietnam, whereas the diversity indices were about the same (around 2.41) for the understories in the two regions. Furthermore, we discovered 18 G. pensilis seedlings at the study sites in Fujian Province. This discovery demonstrates that G. pensilis regeneration is extremely poor and its populations are declining, although these populations are rela-tively healthier than those in Vietnam.

关键词： Age-class Fujian Province Glyptostrobus Regeneration Relict plant Vietnam

Analysis of Global Expression Profiles of Arabidopsis Genes Under Abscisic Acid and H2O2 Applications

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Journal of Integrative Plant Biology 2006年第1期48卷 62-74页

作者： Peng-Cheng wang Yan-Yan Du Guo-Yong An Yun Zhou Chen Miao chun-Peng Song Laboratory of Plant Stress Biology Department of Biology Henan University Kaifeng 475001 China

To gain insight into the coordination of gene expression profiles under abscisic acid （ABA） and H2O2 applications, global changes in gene expression in response to ABA and H2O2 in Arabidopsis seedlings were investigated using GeneChip （Santa Clara, CA, USA） arrays. Among over 24 000 genes present in the arrays, 459 tran- scripts were found to be significantly increased, whereas another 221 decreased following H2O2 treatment compared with control. Similar to treatment with H2O2, ABA treatment elevated the transcription of 391 genes and repressed that of 322 genes. One hundred and forty-three upregulated genes and 75 downregulated genes were shared between the two treatments and these genes were mainly involved in metabolism, signal transduction, transcription, defense, and resistance. Only two genes, which encode an APETALA2/dehydra- tion-responsive element binding protein （AP2/DREBP） family transcriptional factor and a late embryogenesis- abundant protein, were downregulated by H202, but upregulated by ABA. These results suggest that, similar to ABA, H202 plays a global role in gene transcription of Arabidopsis seedlings. The transcriptional responses induced by the application of exogenous ABA and H2O2 overlapped substantially. These two treatments regulated most of the downstream genes in a coordinated manner.

关键词： abscisic acid gene expression hydrogen peroxide microarray.

Cotton Major Latex Protein 28 Functions as a Positive Regulator of the Ethylene Responsive Factor 6 in Defense against Verticillium dahliae

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Molecular Plant 2015年第3期8卷 399-411页

作者： chun-Lin Yang Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Plant Genomics Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

In this study, we identified a defense-related major latex protein （MLP） from upland cotton （designated GhMLP28） and investigated its functional mechanism. GhMLP28 transcripts were ubiquitously present in cotton plants, with higher accumulation in the root. Expression of the GhMLP28 gene was induced by Verticillium dahliae inoculation and was responsive to defense signaling molecules, including ethylene, jas- monic acid, and salicylic acid. Knockdown of GhMLP28 expression by virus-induced gene silencing re- sulted in increased susceptibility of cotton plants to V. dahliae infection, while ectopic overexpression of GhMLP28 in tobacco improved the disease tolerance of the transgenic plants. Further analysis revealed that GhMLP28 interacted with cotton ethylene response factor 6 （GhERF6） and facilitated the binding of GhERF6 to GCC-box element. transient expression assay demonstrated that GhMLP28 enhanced the tran- scription factor activity of GhERF6, which led to the augmented expression of some GCC-box genes. GhMLP28 proteins were located in both the nucleus and cytoplasm and their nuclear distribution was dependent on the presence of GhERF6. Collectively, these results demonstrate that GhMLP28 acts as a positive regulator of GhERF6, and synergetic actions of the two proteins may contribute substantially to protection against V. dahliae infection in cotton plants.

关键词： major latex protein ethylene responsive factor interaction disease tolerance cotton

Real-world Effectiveness and Tolerability of Interferonfree Direct-acting Antiviral for 15,849 Patients with Chronic Hepatitis C:A Multinational Cohort Study

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Journal of Clinical and translational Hepatology 2024年第7期12卷 646-658页

作者： Fanpu Ji Sally tran Eiichi Ogawa chung-Feng Huang Takanori Suzuki Yu Jun Wong Hidenori Toyoda Dae Won Jun Liu Li Haruki Uojima Akito Nozaki Makoto Chuma Cheng-Hao Tseng Yao-chun Hsu Masatoshi Ishigami Takashi Honda Masanori Atsukawa Hiroaki Haga Masaru Enomoto Huy Trinh Carmen Monica Preda Phillip Vutien Charles Landis Dong Hyun Lee Tsunamasa Watanabe Hirokazu Takahashi Hiroshi Abe Akira Asai Yuichiro Eguchi Jie Li Xiaozhong wang Jia Li Junping Liu Jing Liang Carla Pui-Mei Lam Rui Huang Qing Ye Hongying Pan Jiajie Zhang Dachuan Cai Qi wang Daniel Q.Huang Grace Wong Vincent Wai-Sun Wong Junyi Li Son Do Norihiro Furusyo Makoto Nakamuta Hideyuki Nomura Eiji Kajiwara Eileen L.Yoon Sang Bong Ahn Koichi Azuma Kazufumi Dohmen Jihyun An Do Seon Song Hyun Chin Cho Akira Kawano Toshimasa Koyanagi Aritsune Ooho Takeaki Satoh Kazuhiro Takahashi Ming-Lun Yeh Pei-Chien Tsai Satoshi Yasuda Yunyu Zhao Yishan Liu Tomomi Okubo Norio Itokawa Mi Jung Jun Toru Ishikawa Koichi Takaguchi Tomonori Senoh Mingyuan Zhang Changqing Zhao Raluca Ioana Alecu Wei Xuan Tay Pooja Devan Joanne Kimiko Liu Ritsuzo Kozuka Elena Vargas-Accarino Ai-Thien Do Mayumi Maeda Wan-Long Chuang Jee-Fu Huang Chia-Yen Dai Ramsey Cheung Maria Buti Junqi Niu Wen Xie Hong Ren Seng Gee Lim Chao Wu Man-Fung Yuen Jia Shang Qiang Zhu Yoshiyuki Ueno Yasuhito Tanaka Jun Hayashi Ming-Lung Yu Mindie H.Nguyen Department of Infectious Diseases The Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxiChina Division of Gastroenterology and Hepatology Department of MedicineStanford University Medical CenterPalo AltoCAUSA Department of General Internal Medicine Kyushu University HospitalFukuokaJapan Hepatobiliary Division Department of Internal MedicineKaohsiung Medical University HospitalKaohsiung Hepatitis Research Center College of Medicine and Center for Liquid Biopsy and Cohort ResearchKaohsiung Medical UniversityKaohsiung Department of Gastroenterology and Metabolism Nagoya City University Graduate School of Medical SciencesNagoyaJapan Gastroenterology&Hepatology Changi General HospitalSinghealthSingapore Singhealth Duke-NUS Medicine Academic Clinical Program Singapore Department of Gastroenterology Ogaki Municipal HospitalOgakiJapan Department of Internal Medicine Hanyang UniversityCollege of MedicineSeoulKorea Hanyang Institute of Bioscience and Biotechnology Hanyang UniversitySeoulKorea Department of Hepatology The Third People's Hospital of Kunming CityKunmingYunnanChina Department of Gastroenterology Internal MedicineKitasato University School of MedicineSagamiharaJapan Gastroenterological Center Yokohama City University Medical CenterYokohamaJapan Division of Gastroenterology and Hepatology Department of Internal MedicineE-Da Cancer HospitalKaohsiung Department of Gastroenterology and Hepatology Nagoya University Graduate School of MedicineNagoyaJapan Division of Gastroenterology and Hepatology Nippon Medical SchoolTokyoJapan Department of Gastroenterology Yamagata University Faculty of MedicineYamagataJapan Department of transfusion Medicine and Department of Hepatology Osaka Metropolitan University Graduate School of MedicineOsakaJapan San Jose Gastroenterology San JoseCAUSA Gastroenterology&Hepatology Department Clinic Fundeni InstituteBucharestRomania Division of Gastroenterology and Hepatology University of WashingtonSeattleWAUSA Department of

Background and Aims:As practice patterns and hepatitis C virus(HCV)genotypes(GT)vary geographically,a global real-world study from both East and West covering all GTs can help inform practice policy toward the 2030 HCV elimination *** study aimed to assess the effectiveness and tolerability of DAA treatment in routine clinical practice in a multinational cohort for patients infected with all HCV GTs,focusing on GT3 and ***:We analyzed the sustained virological response(SVR12)of 15,849 chronic hepatitis C patients from 39 Real-World Evidence from the Asia Liver Consortium for HCV clinical sites in Asia Pacific,North America,and Europe between 07/01/2014–07/01/***:The mean age was 62±13 years,with 49.6%*** demographic breakdown was 91.1%Asian(52.9%Japanese,25.7%Chinese/Taiwan residents,5.4%Korean,3.3%Malaysian,and 2.9%Vietnamese),6.4%White,1.3%Hispanic/Latino,and 1%Black/***,34.8%had cirrhosis,8.6%had hepatocellular carcinoma(HCC),and 24.9%were treatment-experienced(20.7%with interferon,4.3%with direct-acting antivirals).The largest group was GT1(10,246[64.6%]),followed by GT2(3,686[23.2%]),GT3(1,151[7.2%]),GT6(457[2.8%]),GT4(47[0.3%]),GT5(1[0.006%]),and untyped GTs(261[1.6%]).The overall SVR12 was 96.9%,with rates over 95%for GT1/2/3/6 but 91.5%for ***12 for GT3 was 95.1%overall,98.2%for GT3a,and 94.0%for ***12 was 98.3%overall for GT6,lower for patients with cirrhosis and treatment-experienced(TE)(93.8%)but≥97.5%for tretment-naive patients regardless of cirrhosis *** multivariable analysis,advanced age,prior treatment failure,cirrhosis,active HCC,and GT3/4 were independent predictors of lower SVR12,while being Asian was a significant predictor of achieving ***:In this diverse multinational realworld cohort of patients with various GTs,the overall cure rate was 96.9%,despite large numbers of patients with cirrhosis,HCC,TE,and GT3/***12 for GT3/6 with cirrhosis and TE was lower but st

关键词： Hepatitis C virus Liver cirrhosis Hepatocellular carcinoma Genotype DAA REAL-C

Preparation, Structure and Magnetic Properties of Lithium Substituted NiO by Molten Salt Method

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Chemical Research in Chinese Universities 2013年第2期29卷 210-213页

作者： DU Fei BIE Xiao-fei BIAN Xiao-fei HU Fang CHEN Gang wang chun-zhong WEI Ying-jin Key Laboratory of Advanced Batteries Physics and Technology Ministry of Education College of Physics Jilin University Changchun 130012 P. R. China School of Materials Science and Engineering Changchun University of Science and Technology Changchun 130022 P. R. China School of Materials Science and Engineering Shenyang University of Technology Shenyang 110870 P. R. China

A typical Li＋ substituted NiO compound, Li0.29Ni0.710, was synthesized by molten nitrate method. The ef- fects of Li＋ substitution on the structure and magnetic properties of NiO were investigated. X-Ray diffraction（XRD）, scanning electron microscope（SEM） and high-resolution transmission electron microscope（HRTEM） analyses con- firm the cubic structure of Li0.29Ni0,710, with a primary particle size of 150 nm. Analysis of the Ni X-ray photoelec- tron spectroscopy（XPS） shows the transformation from Ni2. to Ni3＋ induced by Li＋ substitution. Two magnetic tran- sitions were observed at 225 and 55 K which were assigned to the ferrimagnetic ordering and spin glass transition, respectively. The different magnetic behavior with respect to that of NiO was attributed to the break of superexchange interaction Ni2＋-O-Ni2＋ and the formation of different spin clusters after non-magnetic Li＋ doping.

关键词： NiO Magnetic property Substitution effect

transcriptomic analysis reveals the regulation network of BmKruppel homolog 1 in the oocyte development of Bombyx mori

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Insect Science 2021年第1期28卷 47-62页

作者： Zi-Dan Zhu Qi-Hao Hu chun-Mei Tong Hong-Guang Yang Si-chun Zheng Qi-Li Feng Hui-Min Deng Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhouChina

Kriippel homolog 1(Kr-hl),a zinc finger transcription factor,is involved in the metamorphosis and adult reproduction of ***,the role of Kr-hl in reproduction of holometabolic insects remains to be *** regulation network of 7-associated genes in the reproduction in Bombyx mori was investigated in this *** higher expression level of BmKr-hl in the ovaries was detected during the late pupal stage and *** interference(RNAi)-mediated depletion of BmKr-hl in the female at day 6 of pupae resulted in abnormal oocytes at 48 h post-double-stranded RNA treatment,which showed less yolk protein deposition and partially transparent ***-seq and subsequent differentially expressed transcripts analysis showed that knockdown of BmKr-hl caused a decrease in the expression of 2882 genes and an increase in the expression of 2565 genes in the oocytes at day 8 of ***,27 genes coding for transcription factors were down-regulated,while six genes coding for other transcription factors were ***-hl bound to the Kr-hl binding site of the transcription factors AP-1(activating protein-1)and FOXG1 to increase their messenger RNA tran・scripts in the BmN cells,*** Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses of that positively co-expressed with AP-1 and FOXG1 transcripts showed mainly enrichment in the metabolic-related pathways,the nu・tri ent absorption and the yolk protein absorption *** data suggested that BmKr-h 1 might directly regulate the metabolic-related pathways,the nutrient absorption and the yolk protein absorption processes or probably through AP-1 and/or FOXG1 to regulate oocyte development.

关键词： Kriippel homolog 1 oocyte development regulatory network RNA interference transcriptomics

Material and Device Developments of p/n Ⅲ-Nitrde Thin Films made by Low-Temperature Sputtering

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Material and Device Developments of p/n Ⅲ-Nitrde Thin Films...