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Live-cell imaging:new avenues to investigate retinal regeneration

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Neural Regeneration Research 2017年第8期12卷 1210-1219页

作者： Manuela Lahne David R.hyde Department of Biological Sciences University of Notre Dame Notre Dame IN USA

Sensing and responding to our environment requires functional neurons that act in concert. Neuronal cell loss resulting from degenerative diseases cannot be replaced in humans, causing a functional impairment to integrate and/or respond to sensory cues. In contrast, zebrafish（Danio rerio） possess an endogenous capacity to regenerate lost neurons. Here, we will focus on the processes that lead to neuronal regeneration in the zebrafish retina. Dying retinal neurons release a damage signal, tumor necrosis factor α, which induces the resident radial glia, the Müller glia, to reprogram and re-enter the cell cycle. The Müller glia divide asymmetrically to produce a Müller glia that exits the cell cycle and a neuronal progenitor cell. The arising neuronal progenitor cells undergo several rounds of cell divisions before they migrate to the site of damage to differentiate into the neuronal cell types that were lost. Molecular and immunohistochemical studies have predominantly provided insight into the mechanisms that regulate retinal regeneration. However, many processes during retinal regeneration are dynamic and require live-cell imaging to fully discern the underlying mechanisms. Recently, a multiphoton imaging approach of adult zebrafish retinal cultures was developed. We will discuss the use of live-cell imaging, the currently available tools and those that need to be developed to advance our knowledge on major open questions in the field of retinal regeneration.

关键词： multiphoton microscopy live-cell imaging zebrafish interkinetic nuclear migration tissue culture retinal regeneration Miiller glia neuronal progenitor cell differentiation phagocytosis

Epitypification:should we epitypify?

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 842-846页

作者： Kevin D. hyde Ying ZHANG International Fungal Research and Development Centre the Research Institute of Resource Insects Chinese Academy of Forestry Kunming 650224 China Fungal Research Group School of Science Mae Fah Luang University Tasud Muang Chiang Rai 5 7100 Thailand Centre for Research in Fungal Diversity School of Biological Sciences University of Hong Kong Hong Kong China

Epitypification can solve many taxonomic problems and stabilize the understanding of species, genera, families or orders. The aim of this paper is to illustrate how to epitypify. A few examples where taxa have been epitypified are considered and the benefits and disadvantages of epitypification are discussed. We also outline some examples of taxa which need to be epitypifled with reasons.

关键词： Colletotrichum GenBank Molecular phylogeny Taxonomy Typification

Editorial comment–volume 60 of Fungal diversity Special issue dedicated to the COST Action FA1103,“Endophytes in Biotechnology and Agriculture”

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Fungal Diversity 2013年第3期60卷 1-4页

作者： Marc Stadler Kevin D.hyde Department Microbial Drugs Helmholtz-Zentrum für Infektionsforschung GmbHInhoffenstrasse 738124 BraunschweigGermany Institute of Excellence in Fungal Research and School of ScienceMae Fah Luang UniversityChiang RaiThailand

Summary:The European Cooperation in Science and Technology(COST)aims to promote joint interdisciplinary research in various areas and provides substantial funding to international consortia to conduct networking activities such as workshops,training schools,and short time international student *** though primarily European Scientists are eligible to propose COST Actions and to receive funding,the international community can and does *** special issue is dedicated to a COST Action FA1103 on biotechnological and agricultural exploitation of endophytes,entertained by 150 scientists from over 20 *** original papers,one review and two non COST Action papers have been compiled,all of which are dealing with various aspects of fundamental and applied research on fungal *** broad spectrum of the contributions,which are representative of the scientific scope of the Action,is illustrated by reports on innovative methods for all taxa inventories(molecular ecology),studies relating to bioprospecting.

关键词： Action dealing prospecting

Ugliness and judgment

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Ugliness and judgment

350 μm map of the Ophiuchus molecular cloud: core mass function

作者： Timothy hyde

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Science China(Physics,Mechanics & Astronomy) 2015年第2期58卷 114-124页

作者： ZHANG GuoYin LI Di hyde Ashley K QIAN Lei LYU HuaLei WU ZhongZu College of Science/Department of Physics Guizhou University Key Laboratory of Radio Astronomy Chinese Academy of Sciences National Astronomical Observatories Chinese Academy of Sciences Space Science Institute Department of Astronomy California Institute of Technology Astrophysics Group Imperial College London

Stars are born in dense cores of molecular clouds. The core mass function （CMF）, which is the mass distribution of dense cores, is important for understanding the stellar initial mass function （IMF）. We obtained 350μm dust continuum data using the SHARC-II camera at the Caltech Submillimeter Observatory （CSO） telescope. A 350μm map covering 0.25 deg2 of the Ophiuchus molecular cloud was created by mosaicing 56 separate scans. The CSO telescope had an angular resolution of 9＂, corresponding to 1.2 ×103 AU at the distance of the Ophiuchus molecular cloud （131 pc）. The data was reduced using the Comprehensive Reduction Utility for SHARC-II （CRUSH）. The flux density map was analyzed using the GaussClumps algorithm, within which 75 cores has been identified. We used the Spitzer c2d catalogs to separate the cores into 63 starless cores and 12 protostellar cores. By locating Jeans instabilities, 55 prestellar cores （a subcategory of starless cores） were also identified. The excitation temperatures, which were derived from FCRAO 12CO data, help to improve the accuracy of the masses of the cores. We adopted a Monte Carlo approach to analyze the CMF with two types of functional forms; power law and log-normal. The whole and prestellar CMF are both well fitted by a log-normal distribution, with p = -1. 18 ±0.10, σ = 0.58 ± 0.05 and μ= 1.40 ＋ 0.10, σ= 0.50 ＋ 0.05 respectively. This finding suggests that turbulence influences the evolution of the Ophiuchus molecular cloud.

关键词： 1SM molecular clouds Ophiuchus CMF

TOWARDS A NEW MODEL FOR CLIMATE RESPONSIVE DESIGN AT THE UNIVERSITY OF THE SUNSHINE COAST CHANCELLERY

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Journal of Green Building 2009年第3期4卷 3-20页

作者： Peter St.Clair Richard hyde 不详 Faculty of Architecture Design and Planningthe University of SydneyAustralia

INTRODUCTION The ecology and climate of South East Queensland has inspired the development of innovative building design and construction practices for well over a century.A modern regional architecture has evolved since the 1970s where the building form and fabric embrace a climate responsive approach to *** is becoming an increasingly important part of creating ecologically sustainable buildings and development.A core philosophy is to harmonise the building form and fabric with the site and climate thereby reducing ecological impacts and achieving energy effi ciency whilst providing human health and comfort and creating opportunities for social interaction and a productive learning *** University of the Sunshine Coast in Queensland,Australia,has embraced an ecological vision from its *** the first steps of its master planning through to its newest building,the Chancellery,the University demonstrates an evolution of innovation that embodies the principles of climate responsive ecological design.

关键词： thereby steps creating

Fungal diversity on fallen leaves of Ficus in northern Thailand

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作者： Carrie hyde.

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 835-841页

作者： Hong-kai WANG Kevin D. hyde Kasem SOYTONG Fu-cheng LIN Biotechnology Institute Zhejiang University Hangzhou 310029 China Fungal Research Group School of Science Mae Fah Luang University Tasud Chiang Rai 571000 Thailand Faculty of Agricultural TechnologT King Mongkut＇s Institute of Technology Ladkrabang Ladkrabang Bangkok 10520 Thailand

Fallen leaves of Ficus altissima, F. virens, F. benjamina, F. fistulosa and F. semicordata, were collected in Chiang Mai Province in northern Thailand and examined for fungi. Eighty taxa were identified, comprising 56 anamorphic taxa, 23 ascomycetes and l basidiomycete. Common fungal species occurring on five host species with high frequency of occurrence were Beltraniella nilgirica, Lasiodiplodia theobromae, Ophioceras leptosporum, Periconia byssoides and Septonema harknessi. Colletotrichum and Stachybotrys were also common genera. The leaves of different Ficus species supported diverse fungal taxa, and the fungal assemblages on the different hosts showed varying overlap. The fungal diversity of saprobes at the host species level is discussed.

关键词： Ficus Fallen leaves Saprobes Fungal diversity

Advances in the phylogenesis of Agaricales and its higher ranks and strategies for establishing phylogenetic hypotheses

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 779-786页

作者： Rui-lin ZHAO Dennis E. DESJARDIN Kasem SOYTONG Kevin D. hyde Faculty of Biology Conservation Southwest Forestry University Kunming 650224 China Department of Biology San Francisco State University San Francisco California 94132 USA Faculty of Agricultural Technology King Mongkut＇s Institute of Technology Ladkrabang （KMITL） Ladkrabang Bangkok 10520 Thailand International Fungal Resource and Development Centre the Research Institute of Resources Insects Chinese Academy of Forestry Kunming 650224 China Fungal Research Group School of Science Mae Fah Luang University Tasud Chiang Rai 57100 Thailand

We present an overview of previous research results on the molecular phylogenetic analyses in Agaricales and its higher ranks (Agaricomycetes/Agaricomycotina/Basidiomycota) along with the most recent treatments of taxonomic systems in these taxa. Establishing phylogenetic hypotheses using DNA sequences, from which an understanding of the natural evolutionary relationships amongst clades may be derived, requires a robust dataset. It has been recognized that single-gene phylogenies may not truly represent organismal phylogenies, but the concordant phylogenetic genealogies from multiple-gene datasets can resolve this problem. The genes commonly used in mushroom phylogenetic research are summarized.

关键词： Gene Molecular Mushrooms Phylogeny