检索结果-南通市图书馆

A 3D Human Liver Model of Nonalcoholic Steatohepatitis

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

Journal of Clinical and Translational Hepatology 2020年第4期8卷 359-370页

作者： Marion Duriez Agnes Jacquet Lucile Hoet Sandrine Roche Marie-Dominique Bock Corinne Rocher Gilles Haussy Xavier Vigé Zsolt Bocskei Tamara Slavnic Valérie Martin Jean-Claude Guillemot michel didier Aimo Kannt Cécile Orsini Vincent Mikol Anne-Céline Le Fèvre Translational Sciences Sanofi1 Avenue Pierre BrossoletteChilly-MazarinFrance IT&M STATS Groupe IT&MNeuilly-sur-SeineFrance Non Clinical Biostatistics SanofiVitry sur SeineFrance Diabetes and Cardiovascular Research SanofiIndustriepark HöchstFrankfurtGermany Institute of Experimental Pharmacology Medical Faculty MannheimUniversity of HeidelbergMannheimGermany

Background and Aims: To better understand nonalcoholic steatohepatitis (NASH) disease progression and to evaluate drug targets and compound activity, we undertook the devel-opment of an in vitro 3D model to mimic liver architecture and the NASH environment. Methods:We have developed an in vitro preclinical 3D NASH model by coculturing primary hu-man hepatocytes, human stellate cells, liver endothelial cells and Kupffer cells embedded in a hydrogel of rat collagen on a 96-well plate. A NASH-like environment was induced by ad-dition of medium containing free fatty acids and tumor ne-crosis factor-α. This model was then characterized by biochemical, imaging and transcriptomics analyses. Results:We succeeded in defining suitable culture conditions to main-tain the 3D coculture for up to 10 days in vitro, with the lowest level of steatosis and reproducible low level of inflammation and fibrosis. NASH disease was induced with a custom me-dium mimicking NASH features. The cell model exhibited the key NASH disease phenotypes of hepatocyte injury, steatosis, inflammation, and fibrosis. Hepatocyte injury was highlighted by a decrease of CYP3A4 expression and activity, without loss of viability up to day 10. Moreover, the model was able to stimulate a stable inflammatory and early fibrotic environ-ment, with expression and secretion of several cytokines. A global gene expression analysis confirmed the NASH induc-tion. Conclusions:This is a new in vitro model of NASH dis-ease consisting of four human primary cell-types that exhibits most features of the disease. The 10-day cell viability and cost effectiveness of the model make it suitable for medium throughput drug screening and provide attractive avenues to better understand disease physiology and to identify and characterize new drug targets.

关键词： 3D liver model Human primary cells Key features of NASH

COVID-19 patient experiences in prehospital pathways:a processual approach using life-events calendar method and state sequence analysis shows detrimental delays

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Family Medicine and Community Health 2024年第1期12卷 45-56页

作者： Romain Lutaud Sebastien Cortaredona Lea Delorme Patrick Peretti-watel Juliette Mirouse Manon Borg Lucie Cattaneo didier Thery Gaetan Gentile Christian Pradier Touitou Irit Philippe Brouqui Sophie Tardieu michel Carles Stéphanie Gentile Department of General Practice Aix-Marseille UniversityMarseilleFrance ADES MarseilleFrance Institut de Recherche pour le Développement(IRD) MarseilleFrance Assistance Publique-Hôpitaux de Marseille(AP-HM) MarseilleFrance INSERM MarseilleFrance Department of Public Health Archet University HospitalNiceFrance MEPHI MarseilleFrance CEReSS-Health Service Research and Quality of life Center MarseilleFrance Department of Infectious Diseases Centre Hospitalier Universitaire de NiceNiceFrance

Objectives To our best knowledge,no study in France has comprehensively investigated the prehospital history of patients admitted for severe cases of COVID-19.‘Patients’voice is an excellent means to capture data on primary care *** aimed to identify clusters of COVID-19 hospitalised patients with similar prehospital symptom sequences,and to test whether these clusters were associated with a higher risk of poor clinical *** Cross-sectional online survey using life-event *** All patients hospitalised for COVID-19 between September 2020 and May 2021 in the Infectious Disease Departments in Nice and in Marseilles in *** 312 patients responded to the *** outcome measures From the day of symptom onset to the day of hospitalisation,we defined a symptom sequence as the time-ordered vector of the successive symptom grades(grade 1,grade 2,grade 3).State sequence analysis with optimal matching was used to identify clusters of patients with similar symptom *** logistic regressions were performed to test whether these clusters were associated with admission to intensive care unit(ICU)and COVID-19 sequelae after *** Three clusters of symptom sequences were identified among 312 complete prehospital pathways.A specific group of patients(29%)experienced extended symptoms of severe COVID-19,persisting for an average duration of 7.5 days before *** group had a significantly higher probability of being admitted to ICU(adjusted OR 2.01).They were less likely to know a loved one who was a healthcare worker,and more likely to have a lower level of ***,this group of patients,who were more likely to have previously visited the emergency room without exhibiting severe symptoms at that time,may have been inclined to postpone reassessment when their health *** relatives played a decisive role in their *** and relevance This study h

关键词： admitted likely life

Gastrointestinal bleeding and obstructive jaundice: Think of hepatic artery aneurysm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Surgery 2016年第6期8卷 467-471页

作者： Fabrice Vultaggio Pierre-Henri Morère Christophe Constantin michel Christodoulou didier Roulin Service de Chirurgie Generale Hopital du Valais 1950 Sion Switzerland Département d’Imagerie Medicale et Radiologie Interventionnelle Hopital deSierre 1950 Sion Switzerland Service de Chirurgie Hopital de Sierre 3960 Sierre Switzerland

Hemobilia is an uncommon and potential life-threatening condition mainly due to hepato-biliary tree traumatic or iatrogenic *** ruptured aneurysm of the hepatic artery is seldom *** report the case of an 89-year-old woman presenting with abdominal pain,jaundice and gastrointestinal bleeding,whose ultrasound and computed tomography revealed a non-traumatic,spontaneous aneurysm of the right hepatic *** oeso-gastro-duodenoscopy and colonoscopy did not reveal any bleeding at the ampulla of Vater,nor anywhere *** angiography confirmed the diagnosis of hepatic artery aneurysm and revealed a full hepatic artery originating from the superior mesenteric *** patient was successfully treated by selective embolization of *** discuss the etiologies of hemobilia and its treatment with selective embolization,which remains favored over surgical *** aneurysm of the hepatic artery is rare,especially without trauma,a high index of suspicion is needed in order to ensure appropriate treatment.

关键词： Hemobilia Hepatic artery aneurysm Obstructive jaundice Supra-selective micro-embolization Gastrointestinal hemorrhage

Benign retroperitoneal schwannoma presenting as colitis:A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第41期13卷 5521-5524页

作者： Gary Fass didier Hossey michel Nyst Dirk Smets Esmail Najar Saligheh Ruth Duttmann Kathleen Claes Pierre Mendes da Costa Department of Digestive Laparoscopic and Thoracic SurgeryBrugmann University HospitalBrusselsBelgium Department of Radiology Brugmann University HospitalBrusselsBelgium Department of Pathology Brugmann University HospitalBrusselsBelgium Laboratory of Molecular Genetics Centre for Medical GeneticsGhent University HospitalGhentBelgium

We report a case of a patient presenting with clinical,radiological and endoscopic features of colitis due to a compressive left para-aortic mass. Total open surgical excision was performed,which resulted in complete resolution of colitis. Histopathology and immunohistochemistry revealed benign retroperitoneal schwannoma. These neural sheath tumors rarely occur in the retroperitoneum. They are usually asymptomatic but as they enlarge they may compress adjacent structures,which leads to a wide spectrum of non-specific symptoms,including lumbar pain,headache,secondary hypertension,abdominal pain and renal colicky pain. CT and MR findings show characteristic features,but none are specific. Schwannoma can be isolated sporadic lesions,or associated with schwannomatosis or neurofibromatosis type Ⅱ (NF2). Although they vary in biological and clinical behavior,their presence is,in nearly every case,due to alterations or absence of the NF2 gene,which is involved in the growth regulation of Schwann cells. Both conditions were excluded by thorough mutation analysis. Diagnosis is based on histopathological examination and immunohistochemistry. Total excision is therapeutic and has a good prognosis. Schwannomatosis and NF2 should be excluded through clinical diagnostic criteria. Genetic testing of NF2 is probably not justified in the presence of a solitary retroperitoneal schwannoma.

关键词： Colitis Neurofibromatosis Retroperitoneum Schwannoma

Primary Naso-Sinus Tuberculosis (NST) at the ORL and Cervico Facial Surgery Department of the University Hospital of Cocody: About a Case

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Otolaryngology and Head & Neck Surgery 2016年第2期5卷 79-83页

作者： N’gouan J. michel Téa Basilide Koné Samba Chatigre Richard Abounan didier Adjoua Pascal Otolaryngology and Cervico Facial Surgery of the University Hospital of Cocody Abidjan Cote d'Ivoire Traumatology and Orthopedics Surgery of the University Hospital of Cocody Abidjan Cote d'Ivoire Department of Anatomy-Pathology of the University Hospital of Cocody Abidjan Cote d'Ivoire

Objective: To report a case of naso-sinus tuberculosis and discuss its diagnosis and management in our context. Clinical Case: A 37-year-old patient, with no previous history, visited us for a swelling under left orbital including a nasal obstruction and intermittent frontal headaches, which has been developed since about one year. Clinical Examination: The anamnesis founds no notion of epistaxis, visual disability, nor notion of fever. It has been noted a swelling under left angulo orbital during physical examination, a burgeoning mass filling the left nasal including a Lymphadenopathy under homolateral mandibular. Para clinical investigations in particular CT scan of the sinuses have highlighted a tissue process under orbital with a left naso-ethmoido-maxillary extension. The anatomical pathologic examination of the biopsy carried out through endoscopy permitted to highlight epithelioid giganto-cell granulomas with caseous necrosis evoking tuberculosis. The patient has been prescribed an anti-tuberculosis treatment. The course of illness is marked by the healing of the patient.

关键词： Nasal-Sinus Tuberculosis Endoscopy Biopsy

Age of Alpine Corsica ophiolites revisited:Insights from in situ zircon U–Pb age and O–Hf isotopes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Age of Alpine Corsica ophiolites revisited:Insights from in ...

Antimicrobial Characteristics of Untreated Wood: Towards a Hygienic Environment

中国科学院地质与地球物理研究所2015年度（第15届）学术年会

作者： Xian-Hua Li michel Faure Philippe Rossi Wei Lin didier Lahondère State Key Laboratory of Lithospheric Evolution Institute of Geology and GeophysicsChinese Academy of Sciences Institut des Sciences de la Terre d’Orléans Université d’Orléans

Knowledge of the age and timing of ophiolite sequences is essential for understanding the mechanisms of plate *** ophiolites in the Schistes Lustres and the Upper nappes of Alpine Corsica represent remnants of the Liguria-Piemonte ocean basin that formed as a branch of the Central Atlantic basin during the opening of the Mesozoic Western Alpine *** numerous isotopic and paleontological studies,the age and timing of the ophiolites in the Schistes Lustres nappe are still *** study presents integrated in situ analyses of zircon U-Pb age and O-Hf isotopic data for ophiolitic gabbros and plagiogranites from three localities in the Schistes Lustres nappe of Eastern *** new results demonstrate that these rocks crystallized synchronously at-159 Ma,approximately 10 *** than the ophiolites in the Balagne Upper *** from the gabbros and plagiogranites are characterized by highly positiveεHf(t)(+15.0 to+15.9)and mantlelikeδO(5.2-5.4‰)***,these ophiolitic rocks were cogenetic,and crystallized from magmas produced by partial melting of a depleted,N-MORB type *** contrast,in the Balagne Upper nappe,the-169 Ma ophiolites contain numerous xenocrystic zircons inherited from a continental *** current knowledge of isotopic geochronology and geochemistry supports a paleogeographic reconstruction,in which the earliest ophiolites in the Balagne nappe were emplaced close to a continental margin at-169 Ma,while the N-MORB type ophiolites in the Schistes Lustres nappe were likely formed approximately 10 *** in the central part of the Liguria-Piemonte oceanic *** relative location of the Schistes Lustres and Balagne Upper nappes with respect to continental margins is discussed.

关键词： Zircon U–Pb age Zircon O–Hf isotopes Ophiolite Corsica Alpine Tethys Liguria–Piemonte oceanic basin

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Health 2019年第2期11卷 152-170页

作者： Muhammad Tanveer Munir Hélène Pailhories Matthieu Eveillard Florence Aviat didier Lepelletier Christophe Belloncle michel Federighi Laboratoire Innovation Matériau Bois Habitat Apprentissage (LIMBHA) Ecole Supérieure du Bois Nantes France ATOMycA Inserm Equipe Avenir Inserm U892 CNRS 6299 Université Bretagne-Loire Centre Hospitalier Universitaire Angers cedex France Your ResearcH-Bio-Scientific Le Landreau France Laboratoire MiHAR EE 1701 S Institut de Recherche en Santé 2 Université de Nantes 22 boulevard Benoni-Goullin Nantes France UMR INRA 1014 SECALIM Oniris route de Gachet CS 40706 Nantes cedex 03 France

Wood, as a contact surface, has been used for centuries but is usually questioned because of its porosity and organic composition. It has natural antimicrobial properties and, hygienically, can stand the comparison with other materials such as plastic, glass and steel. In this review, we focused on potential microbe-inhibiting properties of wooden surfaces being used in hygienically important places like health institutes and food industries. This article addresses the questionable properties of untreated wood like hygroscopicity, porosity, roughness and chemical composition, and their relation to the hygienic and antimicrobial nature of this material. The other factors linked to the hygienic properties of wood, such as age, species and type of wood, have also been discussed. Our analysis of literature will create better understanding for acceptance of wood as a safety renewable resource. It also provides an outline for future research considering wood material in critical healthcare or food industries.

关键词： Wood Antimicrobial Characteristics Physicochemical Properties

Microbiological Quality Assessment of Drinking Water in Lalo Commune, Benin (West Africa)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Water Resource and Protection 2016年第8期8卷 816-822页

作者： Roch Christian Johnson Gratien Boni Hermione Amoukpo Yves Barogui Gabriel Diez didier Agossadou Ghislain Emmanuel Sopoh michel Boko Laboratory of Hygiene Sanitation Toxicology and Environmental Health Interfaculty Center of Training and Research in Environment for the Sustainable Development University of Abomey-Calavi (UAC) Cotonou Benin National Buruli Ulcer Control Program Ministry of Health Cotonou Benin Anesvad Foundation General Concha Bilbao Spain

Although drinking water is readily available in Benin, its quality presents a public health concern. The district of Ahomadégbé in the commune of Lalo is characterized by several artesian wells. Unfortunately, anthropogenic factors negatively affect the drinking water quality in this area. The aim of this research was to study the microbiological quality of drinking water in the Ahomadégbé district, and to review household water treatment methods employed by the local population. Thirty-five water samples were taken—at water collection points, at selected points along the water transportation system and from water storage facilities, and microbiological parameters were measured. Qualitative data were collected via in-depth interviews with key informants regarding local household water treatment methods. Results reveal that there is a significant degree of microbiological pollution of drinking water in this district, particularly during the water transportation and storage stages where microbiological pollution exceeds levels approved by the World Health Organization. Local residents are familiar with several household water treatment methods. However, these methods are inconsistently applied, which limits their effectiveness. In addition to improving the quality of the drinking water resource itself, it is important to set up interventions relating to water treatment methods in local households.

关键词： Benin Lalo Microbiological Pollution Water Treatment

Hypertensive Pathologies in Peripartum: Complications and Maternal and Neonatal Outcome

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Obstetrics and Gynecology 2020年第2期10卷 311-318页

作者： Isango Idi Yves Criss Koba Mjumbe Kabamba Nzaji michel Malamba Lez didier Kakisingi Ngama Christian Kiopin Mubinda Patrick Mwilambwe Ngoy Steve Manika Muteya michel Ngwe Thaba Jules Mwembo Tambwe Albert Kalenga Muenze Prosper Department of Gynecology and Obstetrics Faculty of Medicine of the University of Lubumbashi Lubumbashi DR Congo Department of Public Health of the University of the University of Lubumbashi Lubumbashi DR Congo Department of Internal Medicine Faculty of Medicine of the University of Lubumbashi Lubumbashi DR Congo.

Introduction: Among the hypertensive pathologies of pregnancy, preeclampsia remains the entity responsible for pregnancy complications. Objective: The aim of this work was to determine the frequency of hypertensive pathologies in the peripartum, to determine the maternal and neonatal morbidity factors associated with preeclampsia on the one hand, and on the other hand, to other forms of hypertension in the peripartal period. Patients and methods: This is a cross-sectional study of hypertensive pathologies in per partum over a period of 15 months?which have been included any pregnant, parturient and hypertensive childbirth. The data was analyzed using SPSS software version 21.0. Data positioning and dispersion parameters were studied. The factor analysis?was?performed by determining the odds ratio with a 95% confidence interval and a significance level set at p?≤ 0.05. Results: 142 cases of hypertension were collected from a total of 2988 deliveries,?i.e.?a frequency of hypertension of 4.8%. The main form of high blood pressure (HBP) was the isolated HBP at 51.4%. 60.3% of primiparas were preeclamptic [OR 2.47 (CI 1.25 - 4.91)], 63.2% of preeclampsia did not follow prenatal consultations?[OR 2.43 (CI 1.06 - 5.62)], 63.6% of preeclampsia had moderate and severe threat premature delivery [OR 4.57 (CI 2.11?-?9.99)], neonatal hypoxia in the fifth minute was found in 34.4% of newborns of preeclamptic mothers [OR 3.02 (CI 1.44?-?6.34)], hypotrophy was observed in 41.4% of the cases in the preeclamptic patients [OR 5.41 (IC 1, from 55 to 19.57)]. Conclusion: Preeclampsia is significantly associated with maternal and neonatal morbidity.

关键词： Hypertensive Pathology PeriPartum Complications Maternal and Neonatal Outcomes