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Transient Expression of Virally Delivered Meganuclease In Planta Generates Inherited Genomic Deletions

Molecular Plant 2015年第8期8卷 1292-1294页

作者： Arik Honig Ira Marton michal rosenthal J. Jeff Smith Michael G. Nicholson Derek Jantz Amir Zuker Alexander Vainstein Danziger Innovations Ltd. Mishmar Hashiva Village Beit Dagan 50297 Israel Precision Biosciences 302 East Pettigrew Street Dibrell Building Suite A-100 Durham NC 27701 USA Institute of Plant Sciences and Genetics in Agriculture Robert H. Smith Faculty of Agriculture Food and Environment Hebrew University of Jerusalem Rehovot 76100 Israel

Dear Editor The use of sequence-specific nucleases for plant genomic DNA mutagenesis is an exciting and rapidly developing tech- nology （Voytas and Merchant, 2013; Baltes and Voytas, 2014）. To date, most mutated plants have been recovered from transgenic plants stably expressing nucleases. However, transient nuclease delivery by plant DNA- and RNA-based viral vectors followed by regeneration of plantlets from modi- fied tissues has emerged as an alternative, efficient strategy in some plant species （Marton et al., 2010; Baltes et al., 2014）. Delivery of nucleases by plant RNA viruses is particularly attractive because they typically do not integrate into the plant genome, and therefore the mutated plants are not classified as transgenic （Marton et al., 2013; Voytas and Gao, 2014）.

关键词： CELLS

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Ion emission from warm dense matter produced by irradiation with a soft x-ray free-electron laser

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Matter and Radiation at Extremes 2024年第1期9卷 71-84页

作者： Josef Krása Tomás Burian Vera Hájková Jaromír Chalupský Šimon Jelínek Katerina Frantálová michal Krupka Zuzana Kuglerová Sushil Kumar Singh Vojtech Vozda Ludek Vysín michalŠmíd Pablo Perez-Martin Marion Kühlman Juan Pintor Jakub Cikhardt Matthias Dreimann Dennis Eckermann Felix rosenthal Sam M.Vinko Alessandro Forte Thomas Gawne Thomas Campbell Shenyuan Ren YuanFeng Shi Trevor Hutchinson Oliver Humphries Thomas Preston Mikako Makita Motoaki Nakatsutsumi Xiayun Pan Alexander Köhler Marion Harmand Sven Toleikis Katerina Falk Libor Juha Department of Radiation and Chemical Physics Institute of PhysicsCzech Academy of SciencesNa Slovance 218221 Prague 8Czech Republic Laser Plasma Department Institute of Plasma PhysicsCzech Academy of SciencesZa Slovankou 318200 Prague 8Czech Republic Department of Surface and Plasma Science Faculty of Mathematics and PhysicsCharles University in PragueV Holesovi ckách 218000 Prague 8Czech Republic Department of Physical Electronics Faculty of Nuclear Science and Engineering PhysicsCzech Technical University in PragueV Holesovi ckách 218000 Prague 8Czech Republic Helmholtz-Zentrum Dresden-Rossendorf Bautzner Landstraße 40001328 DresdenGermany DESY Photon Science Notkestraße 85D-22607 HamburgGermany Institut de Minéralogie de Physique des Matériaux et de CosmochimieUMR 7590–UPMC/CNRS/IRD/MNHNSorbonne Université4 place Jussieu75005 ParisFrance Department of Physics Faculty of Electrical EngineeringCzech Technical University in PragueTechnická216627 Prague 6Czech Republic Center for Soft Nanoscience University of MünsterBusso-Peus-Straße 10D-48149 MünsterGermany Department of Physics Clarendon LaboratoryUniversity of OxfordParks RoadOxford OX13PUUnited Kingdom Central Laser Facility STFC Rutherford Appleton LaboratoryDidcot OX110QXUnited Kingdom Lawrence Livermore National Laboratory 7000 East AvenueLivermoreCalifornia 94550USA European XFEL GmbH Holzkoppel 4D-22869 SchenefeldGermany Technische Universität Dresden 01062 DresdenGermany

We report on an experiment performed at the FLASH2 free-electron laser(FEL)aimed at producing warm dense matter via soft x-ray isochoric *** the experiment,we focus on study of the ions emitted during the soft x-ray ablation process using time-of-flight electron multipliers and a shifted Maxwell–Boltzmann velocity distribution *** find that most emitted ions are thermal,but that some impurities chemisorbed on the target surface,such as protons,are accelerated by the electrostatic field created in the plasma by escaped *** morphology of the complex crater structure indicates the presence of several ion groups with varying *** find that the ion sound velocity is controlled by the ion temperature and show how the ion yield depends on the FEL radiation attenuation length in different materials.

关键词： electrostatic emitted chemi

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Impact of Si on the high-temperature oxidation of AlCr(Si)N coatings

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Journal of Materials Science & Technology 2022年第5期100卷 91-100页

作者： Nikolaus Jager Michael Meindlhumer michal Zitek Stefan Spor Hynek Hruby Farwah Nahif Jaakko Julin Martin rosenthal Jozef Keckes Christian Mitterer Rostislav Daniel Christian Doppler Laboratory for Advanced Synthesis of Novel Multifunctional Coatings at the Department of Materials Science Montanuniversität LeobenAustria voestalpine eifeler Vacotec GmbH DüsseldorfGermany Institute of Ion Beam Physics and Materials Research Helmholtz-Zentrum Dresden-RossendorfDresdenGermany Department of Physics University of JyvaskylaFinland European Synchrotron Radiation Facility GrenobleFrance Erich Schmid Institute for Materials Science Austrian Academy of SciencesAustria Department of Materials Science Montanuniversität LeobenAustria

The resistance of wear protective coatings against oxidation is crucial for their use at high ***,three nanocomposite AlCr(Si)N coatings with a fixed Al/Cr atomic ratio of 70/30 and a varying Si-content of 0 at.%,2.5 at.% and 5 at.% were analyzed by differential scanning calorimetry,thermogravimetric analysis and X-ray in order to understand the oxidation behavior depending on their ***,a partially oxidized AlCrSiN coating with 5 at.%Si on a sapphire substrate was studied across the coating thickness by depth-resolved cross-sectional X-ray nanodiffraction and scanning trans-mission electron microscopy to investigate the elemental composition,morphology,phases and residual stress evolution of the oxide scale and the non-oxidized coating *** results reveal enhanced oxidation properties of the AlCr(Si)N coatings with increasing Si-content,as demonstrated by a retarded onset of oxidation to higher temperatures from 1100℃ for AlCrN to 1260℃ for the Si-containing coatings and a simultaneous deceleration of the oxidation *** annealing of the AlCrSiN sample with5 at.%Si at an extraordinary high temperature of 1400℃ for 60 min in ambient air,three zones developed throughout the coating strongly differing in their composition and structure:(i)a dense oxide layer comprising an Al-rich and a Cr-rich zone formed at the very top,followed by(ii)a fine-grained transition zone with incomplete oxidation and(iii)a non-oxidized zone with a porous *** varying elemental composition of these zones is furthermore accompanied by micro-structural variations and a complex residual stress development revealed by cross-sectional X-ray *** results provide a deeper understanding of the oxidation behavior of AlCr(Si)N coatings depending on their Si-content and the associated elemental,microstructural and residual stress evolution during high-temperature oxidation.

关键词： AlCrSiN Nanocomposite Cathodic arc Oxidation behaviour Cross-sectional X-ray nanodiffraction

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