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Asian Journal of Andrology 2015年第3期17卷 444-446页

作者： michail nomikos karl swann f anthony lai Institute of Molecular and Experimental Medicine School of Medicine Cardiff University Cardiff UK

Mammalian embryo development is initiated by intracellular Ca2＋ oscillations that result in oocyte activation following gamete membrane fusion. It is widely believed that oocyte Ca2＋ oscillations are triggered by a sperm-specific protein, phospholipase C-zeta （PLCζ） that activates InsP3 production leading to repetitive Ca2. release from intracellular stores. However, a recent report in the fASEB Journal by Aarabi et al. challenges this view by proposing postacrosomal WW domain-binding protein （PAWP） as another sperm-derived protein that can also initiate Ca2＋ oscillations and zygotic development at fertilization. Here we discuss these new findings and examine the evidence suggesting PAWP as the ＂real＂ sperm factor.

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Advancing male age differentially alters levels and localization patterns of PLCzeta in sperm and testes from different mouse strains

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Asian Journal of Andrology 2021年第2期23卷 178-187页

作者： Junaid Kashir Bhavesh V Mistry Maha Adel Gumssani Muhammad Rajab Reema Abu-Dawas falah AlMohanna michail nomikos Celine Jones Raed Abu-Dawud Nadya Al-Yacoub Kevin Coward f anthony lai Abdullah M Assiri College of Medicine Alfaisal UniversityRiyadh 11533Saudi Arabia Department of Comparative Medicine King Faisal Specialist Hospital and Research CentreRiyadh 11211Saudi Arabia College of Medicine QU HealthQatar UniversityDoha PO Box 2713Qatar Nuffield Department of Women’s and Reproductive Health University of OxfordLevel 3Women’s CentreJohn Radcliffe HospitalOxford OX39DUUnited Kingdom Biomedical Research Centre Qatar UniversityDoha PO Box 2713Qatar

Sperm-specific phospholipase C zeta(PLCζ)initiates intracellular calcium(Ca2+)transients which drive a series of concurrent events collectively termed oocyte *** investigations have linked abrogation and absence/reduction of PLCζwith forms of male infertility in humans where oocyte activation ***,very few studies have examined potential relationships between PLCζand advancing male age,both of which are increasingly considered to be major effectors of male *** efforts in humans may be hindered by inherent PLCζvariability within the human population,alongside a lack of sufficient controllable ***,utilizing immunoblotting,immunofluorescence,and quantitative reverse transcription PCR(qRT-PCR)we examined for the first time PLCζprotein levels and localization patterns in sperm,and PLCζmRNA levels within testes,from mice at 8 weeks,12 weeks,24 weeks,and 36 weeks of age,from two separate strains of mice,C57BL/6(B6;inbred)and CD1(outbred).Collectively,advancing male age generally diminished levels and variability of PLCζprotein and mRNA in sperm and testes,respectively,when both strains were ***,advancing male age altered the predominant pattern of PLCζlocalization in mouse sperm,with younger mice exhibiting predominantly post-acrosomal,and older mice exhibiting both post-acrosomal and acrosomal populations of PLCζ.However,the specific pattern of such decline in levels of protein and mRNA was ***,our results demonstrate a negative relationship between advancing male age and PLCζlevels and localization patterns,indicating that aging male mice from different strains may serve as useful models to investigate PLCζin cases of male infertility and subfertility in humans.

关键词： ageing fertilization male infertility oocyte activation phospholipase C zeta sperm

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