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Quantification of Zoonotic Bacterial Pathogens within Commercial Poultry Processing Water Samples Using Droplet Digital PCR

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Advances in Microbiology 2013年第5期3卷 403-411页

作者： michael.j. rothrock Kelli L. Hiett Brian H. Kiepper Kim Ingram Arthur Hinton Poultry Processing and Swine Physiology Research Unit USDA-Agricultural Research Service Athens Georgia

Raw poultry and poultry products are a significant source of zoonotic bacterial pathogen transmission;thus the sensitive detection of major zoonotic pathogens (Salmonella spp., Campylobacter jejuni, and Listeria monocytogenes) is a vital food safety issue. Recently, third generation PCR technology, known as droplet digital PCR (ddPCR) has been developed to be more accurate and sensitive to detect genetic targets than current quantification methods, but this technology has not been tested within an industrial setting. There is an on-going study within our laboratory is investigating the effects of sampling times and sampling methods on the cultural and molecular (via qPCR) quantification of dominant zoonotic pathogens within a poultry processing facility. This presents a unique opportunity to compare the quantification resulted from this emerging, third generation technology to traditional quantification methods currently employed by the poultry industry. The results show that ddPCR detected pathogen-specific genes from more pathogen:sampling time combinations than either the qPCR or culturing methods from the final scalder and chiller tanks at three stages of processing (Start, Mid, and End). In fact, both ddPCR and qPCR substantially outperformed culture methods commonly used in poultry processing food safety-related studies, with Salmonella recovered only from the Mid and End sampling times from the scalder tank. While neither C. jejuni nor L. monocytogenes were recovered culturally, ddPCR was able to detect their respective genes commonly throughout the processing day in both the scalder and chiller water samples. Additionally, the use of unfiltered processing water provided significantly greater detection of bacterial and pathogen-specific gene abundances than did an analysis of larger volumes of filtered water. Considering the ddPCR-derived concentrations of the bacterial pathogens were consistent with what was previously found culturally in commercial poultry

关键词： ddPCR Poultry Processing Zoonotic Pathogens qPCR

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IMPROVING THE BIOCOMPATIBILITY OF IN VIVO GLUCOSE BIOSENSORS VIA NITRIC OXIDE RELEASE

IMPROVING THE BIOCOMPATIBILITY OF IN VIVO GLUCOSE BIOSENSORS...

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40th IUPAC Congress

作者： Mark H. Schoenfisch jae Ho Shin Mary E. Robbins Bong Kyun Oh Brian j. Nablo and Aaron R. rothrock Department of Chemistry University of North CarolinaChapel HillNC 27599United States of America Department of Chemistry University of North CarolinaChapel HillNC 27599United States of America Department of Chemistry University of North CarolinaChapel HillNC 27599United States of America Department of Chemistry University of North CarolinaChapel HillNC 27599United States of America Department of Chemistry University of North CarolinaChapel HillNC 27599United States of America Department of Chemistry University of North CarolinaChapel HillNC 27599United States of America

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托吡酯治疗慢性偏头痛患者无效的预测因素

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世界核心医学期刊文摘（神经病学分册） 2005年第11期1卷 32-33页

作者： rothrock j.F. Parada V.A. Drinkard R. 方伯言 Dr. University of South Alabama Headache Center Building 3 3401 Medical Park Drive Mobile AL 36693 United States

Objective. -To identify variables predictive of a negative response to prophylactic therapy with topiramate in patients with chronic migraine. Background. -While certain of the newer antiepileptic drugs (AEDs) have emerged as promising or definitely effective therapies for migraine prevention, we continue to lack biologic or clinical variables predictive of treatment response to these or other widely used prophylactic therapies. Methods. -A consecutive series of 170 patients with IHS defined migraine who were experiencing 15 or more days of headache per month were treated with topiramate according to a uniform dosing protocol. Variables examined for their potential value in predicting treatment response included age, gender, prior experience with prophylactic therapy, prior experience with divalproex sodium specifically, headache frequency and, if present, duration of chronic daily headache (CDH). A positive treatment response was defined as a 50%or greater reduction in headache days during the second treatment month relative to the patient’s pretopiramate baseline. Only patients who completed the treatment phase and achieved the 50 mg BID target dose were analyzed (efficacy analysis). Each variable prospectively selected was evaluated in regards to treatment outcome via a paired Mest, and a multiple regression analysis of all variables subsequently was performed. Results. -A total of 116 patients completed at least 60 days of treatment and consequently were available for analysis. In the efficacy analysis, 45 (38.8%) of the 116 responded positively to topiramate. Neither age nor gender influenced treatment response. Those patients with CDH of more than 6 months duration, patients who previously had tried and failed more than three prophylactic agents and patients who previously had failed to respond to divalproex sodium were more likely to be nonresponders, but after multiple regression analysis the only statistically significant predictor of a negative treatment r

关键词：偏头痛患者托吡酯双丙戊酸钠抗癫痫药物症状持续时间慢性每日头痛药物反应用药史预测变量反应变量

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北美大豆籽粒、豆荚和花器上真菌区系分析及重要真菌鉴定方法和检索表

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大豆科技 2015年第1期 34-34页

作者：李雪光 M.T.Kirkpatrick j.C.Rupe C.S.rothrock Department of Plant Pathology University of Arkansas

文章综述了北美大豆籽粒、豆荚或花器上的真菌。半知菌纲的真菌在每个大豆花器官中是最常见的真菌,其次为占真菌区系总数四分之一的子囊菌纲和藻菌纲。在大豆籽粒、豆荚或花器上鉴定出了80个属、至少135种的真菌。各个器官上的真菌数量... 详细信息

文章综述了北美大豆籽粒、豆荚或花器上的真菌。半知菌纲的真菌在每个大豆花器官中是最常见的真菌,其次为占真菌区系总数四分之一的子囊菌纲和藻菌纲。在大豆籽粒、豆荚或花器上鉴定出了80个属、至少135种的真菌。各个器官上的真菌数量分布为,在籽粒上有63属和大约108种以上真菌,在豆荚上有65属和大约88种以上真菌,在花器上有36属和大约至少47个种真菌,而且在花器上发现的真菌大多数可以从豆荚上分离到，花器上这些真菌的大多数也发生在籽粒上。建立了30种最重要真菌的鉴定方法和检索表。

关键词：真菌区系大豆籽粒鉴定方法花器官检索表豆荚区系分析北美

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大豆对土壤水淹的响应及与土传植物病原真菌的关系

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大豆科技 2015年第1期 33-33页

作者：向新华 M.T.Kirkpatrick j.C.Rupe C.S.rothrock Department of Plant Pathology University of Arkansas

选择被认为对水淹有较好抗性的6个大豆基因型品种,检测其在洪水灾害下土传真菌对大豆的作用。试验在1996年到1998年进行,6个基因型大豆进行垄上单行种植,设置不同水淹处理,分别为无水淹、出苗时水淹（持续3 d）和第四个叶节期水淹（持续... 详细信息

选择被认为对水淹有较好抗性的6个大豆基因型品种,检测其在洪水灾害下土传真菌对大豆的作用。试验在1996年到1998年进行,6个基因型大豆进行垄上单行种植,设置不同水淹处理,分别为无水淹、出苗时水淹（持续3 d）和第四个叶节期水淹（持续7 d）。在水淹处理3~4 d后,统计出苗率,并在每个小区采集15株大豆样本,进行称重,并调查根部褪色比率,分离鉴定根上真菌及其他丝状微生物。试验结果显示,与无淹水相比在出苗期淹水导致出苗率降低。

关键词：植物病原真菌水淹大豆基因型品种土壤丝状微生物出苗率洪水灾害

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终极腐霉(Pythium ultimum)和土壤淹水对两个大豆品种的影响

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大豆科技 2015年第1期 37-37页

作者：向新华 M.T.Kirkpatrick C.S.rothrock j.C.Rupe E.E.Gbur .Department of Plant Pathology Agriculture Statistics Lab University of Arkansas Fayetteville 72701

采用2个大豆品种Hutcheson和Archer,通过温室一系列试验研究了水淹和终极腐霉（Pythium ultimum）对大豆的影响。一个试验是,大豆种子播种到经过灭菌土壤中,一部分土壤里接种沙子-玉米面的终极腐霉接菌体使之侵染,另一部分未接种病原菌... 详细信息

采用2个大豆品种Hutcheson和Archer,通过温室一系列试验研究了水淹和终极腐霉（Pythium ultimum）对大豆的影响。一个试验是,大豆种子播种到经过灭菌土壤中,一部分土壤里接种沙子-玉米面的终极腐霉接菌体使之侵染,另一部分未接种病原菌;水淹处理是分别在出苗期水淹2 d、4叶节期（V4）水淹5 d,在每个试验中都有一个没有水淹的对照处理。

关键词：大豆品种终极腐霉土壤淹水种子播种水淹试验玉米面

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