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国际心血管病杂志 1986年第2期 103-105页

作者： shapiro W 舒晓东

Bepridil 是一种新型钙通道拮抗剂,具亲脂质性,重复给药时半衰期为42±12小

关键词：慢性稳定型心绞痛用药硝酸甘油 Bepridil 安慰剂空白剂

DNA致癌病毒的诱变作用和哺乳类细胞的恶性转化

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国际遗传学杂志 1985年第6期08卷 310-313页

作者： shapiro NI 周福纲

七十年代初,许多研究结果证实了癌的突变学说。这些结果提示,诱变参与细胞的恶性转化过程,并且可能存在某些突变引致恶性转化的基因。目前,已从分子水平证实了原癌基因的存在。由于病毒将其自身遗传信息引入细胞而使恶性转化的情况更为... 详细信息

七十年代初,许多研究结果证实了癌的突变学说。这些结果提示,诱变参与细胞的恶性转化过程,并且可能存在某些突变引致恶性转化的基因。目前,已从分子水平证实了原癌基因的存在。由于病毒将其自身遗传信息引入细胞而使恶性转化的情况更为复杂。目前尚无实验证据证实突变在病毒致癌中的作用。致癌病毒是否诱导宿主细胞的基因突变也不清楚。事实上,关于病毒诱导脊椎动物的基因突变亦无可利用的资料。

关键词：致癌病毒哺乳类细胞病毒诱导 DNA 恶性转化

组成性雄烷受体(CAR)对细胞色素酶CYP2B6表达调控的信号通路研究

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组成性雄烷受体(CAR)对细胞色素酶CYP2B6表达调控的信号通路研究

新型化学反应法制备SiC/Al_2O_3复相陶瓷涂层

中国毒理学会第四届中青年学者科技论坛

作者：杨辉 Brandy Garzel Scott Heyward Timothy Moeller Paul shapiro 王红兵国家食品安全风险评估中心 Department of Pharmaceutical Sciences School of Pharmacy University of Maryland

目的细胞色素P450酶(CYP450)能够参与多种内、外源性化合物的生物转化,具有解毒或代谢活化等重要生物学功能;研究核受体对CYP450的调节作用对于描述毒物、药物代谢特征以及药物相互作用具有重要意义。本研究拟探讨组成型雄烷受体(consti...

目的细胞色素P450酶(CYP450)能够参与多种内、外源性化合物的生物转化,具有解毒或代谢活化等重要生物学功能;研究核受体对CYP450的调节作用对于描述毒物、药物代谢特征以及药物相互作用具有重要意义。本研究拟探讨组成型雄烷受体(constitutive androstane receptor,CAR)对人类肝脏细胞色素酶CYP2B6表达的调节作用及信号转导机制。方法采用体外培养方法对人原代肝细胞进行药物处理:溶剂对照(0.1%DMSO)、苯巴比妥(PB,1 mM)、CITCO(1uM)或/和二甲双胍(MET,0.1、0.5、1 mM),处理时间为15 min2 h;分别采用RTPCR和Western blotting检测CYP2B6 mRNA和蛋白表达水平,通过双荧光素酶报告基因试验检测CAR活化水平;构建能够表达增强型黄色荧光蛋白标记CAR的腺病毒并感染肝细胞,通过共聚集显微镜观察CAR在肝细胞内的分布和转移;通过磷酸化Western blotting检测AMPK、ERK1/2、p38 MAPK、EGFR等信号通路对CAR磷酸化水平的影响。结果 MET能够显著抑制由PB和CITCO诱导的CYP2B6基因表达;机制研究表明,MET能够通过激活AMPK信号通路使CAR发生磷酸化,从而阻断其活化及向细胞核转移过程;通过对潜在信号调节通路的对比研究发现,ERK1/2在CAR信号调节机制中具有一定作用而p38 MAPK和EGFR对其无显著影响;AMPK和ERK1/2均无法单独解释CYP2B6基因表达的抑制效应,提示其他信号转导通路对CAR的激活产生交叉调控作用。结论 AMPK信号转导通路是CAR活化及CYP2B6基因表达的主要调控途径;基于CYP2B6对药物和毒物代谢的重要作用,CAR的其他信号调节机制及其与已知通路的交互影响有待进一步识别和验证;临床应用中MET对其他联合用药的潜在影响值得关注。

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中国表面工程 2009年第6期22卷 24-29页

作者：肖平杨大祥赵晓峰 Ian shapiro Eddie Lopez Honorato 宋永才曼彻斯特大学材料学院材料工程中心装甲兵工程学院装备再制造技术国防科技重点实验室北京100072 国防科技大学新型陶瓷纤维及其复合材料国防科技重点实验室长沙410073

陶瓷涂层材料以其高硬度、耐腐蚀、耐高温、抗氧化等一系列优点,在航空航天、武器装备、核工业、石油化工等领域具有广阔的应用前景。SiC/Al2O3复相陶瓷在常温和高温下都具有较好耐磨性和化学稳定性,是一种极具工程应用前景的复合陶瓷... 详细信息

陶瓷涂层材料以其高硬度、耐腐蚀、耐高温、抗氧化等一系列优点,在航空航天、武器装备、核工业、石油化工等领域具有广阔的应用前景。SiC/Al2O3复相陶瓷在常温和高温下都具有较好耐磨性和化学稳定性,是一种极具工程应用前景的复合陶瓷材料。本研究采用优质陶瓷先驱体聚合物PCS,添加惰性填料SiC纳米颗粒和活性填料金属Al粉共同作用,克服了先驱体法制备陶瓷材料中存在较大体积收缩的缺点,在金属基体上制备了单层厚度可达50μm的致密SiC/Al2O3复相陶瓷涂层,复相陶瓷涂层中的SiC来自于先驱体PCS热解转化的SiC和添加的纳米SiC颗粒;其中的Al2O3来自于金属Al粉的氧化。该化学反应方法在金属基体上成功地制备出连续、致密的SiC/Al2O3复相陶瓷涂层,既简单又方便,表现出极大的应用潜力。

关键词：先驱体法碳化硅氧化铝复相陶瓷涂层

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依前列醇长期治疗原发性肺动脉高压的临床资料分析

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中华内科杂志 2003年第2期42卷 106-109页

作者：曹铁生袁丽君段云友王作军庄磊 Shelley M shapiro Bruce H Brundage 第四军医大学唐都医院超声诊断科西安710038 Harbor UCLA Medical Center TorranceCA

目的　观察依前列醇长期治疗原发性肺动脉高压 (PPH)的疗效。方法　临床诊断为PPH且心功能为Ⅲ～Ⅳ级的慢性充血性心力衰竭患者 69例 ,均接受依前列醇长期静脉连续注入治疗(起始剂量在 2～ 62ng·kg- 1 ·min- 1 范围内。在长期治疗中 ... 详细信息

目的　观察依前列醇长期治疗原发性肺动脉高压 (PPH)的疗效。方法　临床诊断为PPH且心功能为Ⅲ～Ⅳ级的慢性充血性心力衰竭患者 69例 ,均接受依前列醇长期静脉连续注入治疗(起始剂量在 2～ 62ng·kg- 1 ·min- 1 范围内。在长期治疗中 ,药物剂量应根据患者的反应进行调整 ,剂量通常随治疗时间每 2个月增加 1次 ,每次增加 1～ 2ng·kg- 1 ·min- 1 )并观察其疗效。用右心导管法和超声心动图检查法对所有患者测量三尖瓣跨瓣压 (ΔP)和心输出量 (CO)。随访期间每 4个月用超声心动图检查法复查上述指标 1次。结果　PPH患者长期用依前列醇治疗后 ,ΔP由 (84 1± 2 4 1 )mmHg (1mmHg=0 1 33kPa)降至 (62 7± 1 8 2 )mmHg(P <0 0 1 ) ,CO由 (4 0 0± 1 2 2 )L/min升至 (4 70± 1 2 7)L/min(P <0 0 2 ) ,ΔP/CO由 2 2 8± 9 4降至 1 4 9± 6 5(P <0 0 1 )。结论　长期静脉连续注入依前列醇治疗PPH ,可使患者右室压力逐渐下降 ,CO增加。

关键词：依前列醇治疗原发性肺动脉高压

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Association between quality of life and anxiety, depression, physical activity and physical performance in maintenance hemodialysis patients

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Chronic Diseases and Translational Medicine 2016年第2期2卷 -页

作者： Yi-Nan Li Bryan shapiro Jun Chul Kim Min Zhang Janos Porszasz Usama Feroze Division of Nephrology and Hypertension Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center Torrance CA 90502 USADepartment of Nephrology The First Affiliated Hospital of Xiamen University Xiamen Fujian 361003 China Division of Nephrology and Hypertension Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center Torrance CA 90502 USA Division of Nephrology and Hypertension Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center Torrance CA 90502 USADivision of Nephrology CHA Gumi Medical Center CHA University Kyungsangbuk-do 730728 South Korea Division of Nephrology and Hypertension Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center Torrance CA 90502 USADivision of Nephrology Tianjin Union Medical Center Tianjin 300121 China Rehabilitation Clinical Trials Center Division of Respiratory&Critical Care Physiology&Medicine Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center Torrance CA 90502 USA George Washington University/Inova Fairfax Hospital Psychosomatic Fellowship Program Washington DC 20052 USA

OBJECTIVE:Maintenance hemodialysis (MHD) patients often have impaired quality of life (QOL), anxiety, depression, and reduced daily physical activity (DPA) and physical performance. The contributions of these latter factors to reduced QOL in MHD are poorly understood. We examined the association of QOL with anxiety, depression, DPA, and physical performance.;METHODS:Seventy-two relatively healthy adult MHD patients, vintage ≥6 months, and 39 normals of similar age range and gender distribution were studied. QOL was assessed using the Kidney Disease Quality of Life-Short Form (KDQOL-SF). Anxiety and depression were each evaluated with two questionnaires. DPA and physical performance were assessed with a physical activity monitor, Human Activity Profile, and 6-minute walk, sit-to-stand, and stair-climbing tests.;RESULTS:Most KDQOL components were reduced in MHD patients versus normals. KDQOL components in patients were commonly inversely correlated with measures of anxiety and depression ( < 0.05) and were more reduced in patients with both anxiety and depression. KDQOL was often impaired in patients with either anxiety or depression. However, most KDQOL scores did not differ between patients and normals without anxiety or depression. DPA, Human Activity Profile, and physical performance often correlated with KDQOL scores in adjusted models, but after further adjustment for anxiety and depression, DPA, Human Activity Profile, and physical performance correlated less frequently with KDQOL scores. This reduction in significant correlations after adjustment for anxiety and depression was particularly pronounced for the association between KDQOL and DPA.;CONCLUSIONS:In relatively healthy MHD patients, KDQOL scores are usually decreased in those with anxiety and/or depression but are usually normal in those without anxiety or depression. Lower DPA in MHD patients with reduced KDQOL scores often appears to be associated with anxiety and depression. The relationship between

关键词： Anxiety Depression Kidney disease Kidney failure Quality of life

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Hypoglycemic myocardial stunning as cause of cardiogenic shock in a patient with ischemic cardiomyopathy: A case report and review of literature

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Case Reports in Clinical Medicine 2013年第1期2卷 89-92页

作者： Khawar Maqsood Ghazi Mirrani Nosheen Sarwar Amatur R. Amarah Muhammad Rizwan Sardar Timothy A. shapiro Department of Medicine Baystate Medical Center Tufts University School of Medicine Springfield USA Department of Medicine Division of Cardiology Lankenau Medical Center Wynnewood USA Department of Medicine Division of Internal Medicine Lankenau Medical Center Wynnewood USA

Hypoglycemia is a common complication seen in patients with diabetes mellitus and has been proven to have adverse effects on cardiovascular mortality. Hypoglycemia can potentially lead to worsening of cardiac function in patients with ischemic heart disease. We present a case of cardiogenic shock in a patient with hypoglycemia secondary to insulin accumulation due to worsening renal function with dramatic recovery of shock once his sugars normalized.

关键词： Chronic Heart Failure Hypoglycemia Myocardial Stunning Ischemic Cardiomyopathy Cardiogenic Shock

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并行存储器中的存储方案

计算机研究与发展 1978年第2期 57-63页

作者： H.D.shapiro ,宋德芬

考虑了在多处理计算系统中的独立存储体中数据存储的各种斜移方案。介绍了有关同时要求存储器利用率为百分之百,而访问公共存储阵列部分时又不碰头的结果。表明在一定条件下,这两个要求都不能满足。而在另外的条件下,线性斜移就够了,而... 详细信息

考虑了在多处理计算系统中的独立存储体中数据存储的各种斜移方案。介绍了有关同时要求存储器利用率为百分之百,而访问公共存储阵列部分时又不碰头的结果。表明在一定条件下,这两个要求都不能满足。而在另外的条件下,线性斜移就够了,而非线性斜移不提高性能。

关键词：周期性除尽库克尼克对角线存储器存贮器计算机

新型化学反应法在金属基底上制备SiC/Al2O3复相陶瓷涂层

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新型化学反应法在金属基底上制备SiC/Al2O3复相陶瓷涂层

Superior Conjugative Plasmids Delivered by Bacteria to Diverse Fungi

第八届全国表面工程学术会议暨第三届青年表面工程学术论坛

作者：肖平杨大祥赵晓峰 Ian shapiro Eddie Lopez Honorato 宋永才曼彻斯特大学材料学院材料工程中心装甲兵工程学院装备再制造技术国防科技重点实验室国防科技大学新型陶瓷纤维及其复合材料国防防科技重点实验室

陶瓷涂层材料以其高硬度、耐腐蚀、耐高温、抗氧化等一系列优点,在航空航天、武器装备、核工业、石油化工等领域具有广阔的应用前景。SiC/Al2O3复相陶瓷在常温和高温下都具有较好耐磨性和化学稳定性,是一种极具工程应用前景的复合陶瓷材料。本研究采用优质陶瓷先驱体聚合物PCS,添加惰性填料SiC纳米颗粒和活性填料金属Al粉共同作用,克服了先驱体法制备陶瓷材料中存在较大体积收缩的缺点,在金属基底上制备了单层厚度可达50μm的致密SiC/Al2O3复相陶瓷涂层,复相陶瓷涂层中的SiC来自于先驱体PCS热解转化的SiC和添加的纳米SiC颗粒;其中的Al2O3来自于金属Al粉的氧化。该化学反应方法在金属基底上成功地制备出连续、致密的SiC/Al2O3复相陶瓷涂层,既简单又方便,表现出极大的应用潜力。

关键词：先驱体法碳化硅氧化铝复相陶瓷涂层

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BioDesign Research 2022年第1期2022卷 151-165页

作者： Ryan R.Cochrane Arina Shrestha Mariana M.Severo de Almeida Michelle Agyare-Tabbi Stephanie L.Brumwell Samir Hamadache Jordyn S.Meaney Daniel P.Nucifora Henry Heng Say Jehoshua Sharma Maximillian P.M.Soltysiak Cheryl Tong Katherine Van Belois Emma J.L.Walker Marc-AndréLachance Gregory B.Gloor David R.Edgell Rebecca S.shapiro Bogumil J.Karas Department of Biochemistry Schulich School of Medicine and DentistryThe University of Western OntarioLondonONCanada N6A 5C1 Department of Molecular and Cellular Biology University of GuelphGuelphONCanada N1G 2W1 Department of Biology The University of Western OntarioLondonOntarioCanada N6A 5B7

Fungi are nature’s recyclers,allowing for ecological nutrient cycling and,in turn,the continuation of life on *** fungi inhabit the human microbiome where they can provide health benefits,while others are opportunistic pathogens that can cause ***,members of the fungal kingdom,have been domesticated by humans for the production of beer,bread,and,recently,medicine and ***,the great untapped potential exists within the diverse fungal ***,many yeasts are intractable,preventing their use in biotechnology or in the development of novel treatments for pathogenic ***,as a first step for the domestication of new fungi,an efficient DNA delivery method needs to be ***,we report the creation of superior conjugative plasmids and demonstrate their transfer via conjugation from bacteria to 7 diverse yeast species including the emerging pathogen Candida *** create our superior plasmids,derivatives of the 57 kb conjugative plasmid pTA-Mob 2.0 were built using designed gene deletions and insertions,as well as some unintentional ***,a cluster mutation in the promoter of the conjugative gene traJ had the most significant effect on improving conjugation to *** addition,we created Golden Gate assembly-compatible plasmid derivatives that allow for the generation of custom plasmids to enable the rapid insertion of designer genetic ***,we demonstrated that designer conjugative plasmids harboring engineered restriction endonucleases can be used as a novel antifungal agent,with important applications for the development of next-generation antifungal therapeutics.

关键词： insertion continuation harbor