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Journal of Microbiology and Immunology 2004年第4期2卷 272-277页

作者：杨建民 michael S FrIEDMAN Christopher M rEYNOLDS Marianne T HUBEN Lee WILKE Jennifer FULLEr 李桥 Zelig ESHHAr James J MULE Kevin T MCDONAGH Department of Hematology Changhai Hospital The Second Millitay Medical University Shanghai 200433 China Departments of Internal Medicine Departments of Surgery Departments of Internal Medicine University of Michigan Medical School Ann Arbor MI 48109 and De- partment of Chemical Immunology the Weizmann Institute of Science Israel

We have developed and tested chimeric T-cell receptors (TCr) specific for p185HEr2. In these experiments, retroviral vectors expressing the N29γ or N29ζ receptors were constructed in prET6. Amphotropic viral producer cells were established in the GALV-based PG13 packaging cell line. Ficoll purified human peripheral blood lymphocytes (PBL) were virally transduced using an optimized protocol incorporating activation with immobilized anti-CD3/anti-CD28 monoclonal anti- bodies, followed by viral infection in the presence of fibronectin fragment CH296. Transduced cells were co-cultured with human tumor cell lines that overexpress (SK-OV-3) or underexpress (MCF7) p185HEr2 to assay for antigen specific im- mune responses. Both CM+ and CD8+ T-cells transduced with the N29γ or N29ζ chTCr demonstrated HEr2-specific anti- gen responses, as determined by release of Th1 like cytokines, and cellular cytotoxicity assays. Our results support the fea- sibility of adoptive immunotherapy with genetically modified T-cells expressing a chTCr specific for p185HEr2.

关键词： Gene therapy retrovirus Chimeric T-cell receptor Human peripheral T-lymphocytes Immunotherapy

Tumor necrosis factor receptor superfamily member 9 is upregulated in the endothelium and tumor cells in melanoma brain metastasis

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Neuroimmunology and Neuroinflammation 2014年第1期1卷 135-140页

作者： Patrick N.Harter Anna-Eva Blank Benjamin Weide rudi Beschorner Simon Bernatz Peter Baumgarten Anne KBraczynski Elke Hattingen michael Wronellenfitsch Herbert Schwarz Michel Mittelbronn Institute of Neurology(Edinger Institute) Goethe University60528 FrankfurtGermany German Cancer consortium(DKTK) 69120 HeidelbergGermany German Cancer research Center(DKFZ) 69120 HeidelbergGermany Department of Neuropediatrics University of Frankfurt60590 FrankfurtGermany Department of Dermatology Eberhardt-Karls University72076 TuebingenGermany Department of Neuropathology Institute of Pathology and NeuropathologyEberhardt-Karls University72076 TuebingenGermany Department of Neurosurgery Goethe University60528 FrankfurtGermany Department of Neuroradiology University of Frankfurt am Main60258 FrankfurtGermany Dr.Senckenberg Institute of Neurooncology University of Frankfurt am Main60258 FrankfurtGermany Department of Physiology Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117456Singapore

Aim:The cytokine receptor tumor necrosis factor receptor superfamily member 9(TNFrSF9)is mainly considered to be a co-stimulatory activation marker in hematopoietic *** preclinical models have shown a dramatic beneficial effect of treatment approaches targeting TNFrSF9 with agonistic ***,preliminary clinical phase I/II studies were stopped after the occurrence of several severe deleterious side *** a previous study,it was demonstrated that TNFrSF9 was strongly expressed by reactive astrocytes in primary central nervous system(CNS)tumors,but was largely absent from tumor or inflammatory *** aim of the present study was to address the cellular source of TNFrSF9 expression in the setting of human melanoma brain metastasis,a highly immunogenic tumor with a prominent tropism to the ***:Melanoma brain metastasis was analyzed in a cohort of 78 patients by immunohistochemistry for TNFrSF9 and its expression was co.related with clinicopathological parameters including sex,age,survival,tumor size,number of tumor spots,and BrAF V600E expression ***:Tumor necrosis factor receptor superfamily member 9 was frequently expressed independently on both melanoma and endothelial *** addition,TNFrSF9 was also present on smooth muscle cells of larger vessels and on a subset of lymphomonocytic tumor *** association between TNFrSF9 expression and patient survival or other clinicopathological parameters was *** note,several cases showed a gradual increase in TNFrSF9 expression on tumor cells with increasing distance from blood vessels,an observation that might be linked to hypoxia-driven TNFrSF9 expression in tumor ***:The findings indicate that the cellular source of TNFrSF9 in melanoma brain metastasis largely exceeds the lymphomonocytic pool,and therefore further careful(re-)assessment of potential TNFrSF9 functions in cell types other than hematopoietic cells is ***,the hypothesis of

关键词： 4-1BB brain metastasis CD137 melanoma tumor necrosis factor receptor superfamily member 9

Molecular Characterization of the Calvin Cycle Enzyme Phosphoribulokinase in the Stramenopile Alga Vaucheria litorea and the Plastid Hosting Mollusc Elysia chlorotica

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Molecular Plant 2009年第6期2卷 1384-1396页

作者： Mary E. rumpho Sirisha Pochareddy Jared M. Worful Elizabeth J. Summer Debashish Bhattacharya Karen N. Pelletreau Mary S. Tyler Jungho Lee James r. Manhart Kara M. Soule Department of Biochemistry Microbiology and Molecular Biology 5735 Hitchner Halt University of Maine Orono ME 04469 USA Department of Biology Texas A&M University College Station TX 77843 USA Department of Ecology Evolution and Natural Resources Institute of Marine and Coastal Sciences Rutgers University New Brunswick NJ 08901 USA School of Biology and Ecology University of Maine Orono ME 04469 USA Green Plant Institute #2-202 Bio Valley Seoul National University 103-2 Seodun Gwonseon Suwon Gyeonggi 441-853 Korea Department of Biochemistry and Biophysics Texas A&M University College Station TX 77843 USA

Phosphoribulokinase （PrK）, a nuclear-encoded plastid-localized enzyme unique to the photosynthetic carbon reduction （Calvin） cycle, was cloned and characterized from the stramenopile alga Vaucheria litorea. This alga is the source of plastids for the mollusc （sea slug） Elysia chlorotica which enable the animal to survive for months solely by photoautotrophic co2 fixation. The 1633-bp V. litorea prk gene was cloned and the coding region, found to be interrupted by four introns, encodes a 405-amino acid protein. This protein contains the typical bipartite target sequence expected of nuclearencoded proteins that are directed to complex （i.e. four membrane-bound） algal plastids. De novo synthesis of PrK and enzyme activity were detected in E. chlorotica in spite of having been starved of V. litorea for several months. Unlike the algal enzyme, PrK in the sea slug did not exhibit redox regulation. Two copies of partial PrK-encoding genes were isolated from both sea slug and aposymbiotic sea slug egg DNA using PCr. Each copy contains the nucleotide region spanning exon 1 and part of exon 2 of V litorea prk, including the bipartite targeting peptide. However, the larger prk fragment also includes intron 1. The exon and intron sequences of prk in E. chlorotica and V/itorea are nearly identical. These data suggest that PrK is differentially regulated in V. litorea and E. chlorotica and at least a portion of the V. litorea nuclear PrK gene is present in sea slugs that have been starved for several months.

关键词： Alga Calvin cycle Elysia chlorotica kleptoplast mollusc phosphoribulokinase photosynthesis plastid redox regulation stramenopile symbiosis Vaucheria litorea.

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中国进行低成本co2埋存示范项目的机遇

石油科技动态 2009年第11期 22-36页

作者： Kyle C. Meng robert H. Williams michael A. Celia

为了证明co2捕集和埋存是缓解气候变化的一个主要措施，要在全世界各种不同的地质储层中开展co2埋存示范项目。在中国，通过煤气化生产NH，的工厂有一些低成本的co2气源。在这些工厂，因产生的co2超过其他生产目的（例如合成尿素）的所... 详细信息

为了证明co2捕集和埋存是缓解气候变化的一个主要措施，要在全世界各种不同的地质储层中开展co2埋存示范项目。在中国，通过煤气化生产NH，的工厂有一些低成本的co2气源。在这些工厂，因产生的co2超过其他生产目的（例如合成尿素）的所需量，多余的co2就作为一种相对纯净的气体排出。如果附近有适当的埋存地点，这些co2气体可作为埋存示范项目的具有经济吸引力的潜在埋存对象。本研究调查和评估了中国现在燃煤合成氨厂co2的可用程度。结果表明，单个工厂每年可提供相对纯净的co2量为0．6-1．1Mt。分析了在co2气源附近进行地质埋存的可行性。把co2气源的地理位置标示在可能存在适合co2埋存油气藏的中国产油沉积盆地中，发现了四对有前景的co2气源和埋存地点。深部盐水层埋存的工程费用预计为$15-21／tco2。此外，还评估了气源附近提高石油采收率和煤层气采收率的潜力。

关键词： co2气体示范项目中国产低成本石油采收率合成氨厂气候变化沉积盆地

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铸铁熔炼热分析技术发展九十年

铸造 2021年第2期70卷 231-247页

作者： STEFANESCU Doru michael SUArEZ ramon KIM Sung bin The Ohio State University ColumbusOH and The University of AlabamaTuscaloosaAL IK4-Azterlan Durango(Bizkaia)Spain Veigalan Estudio 2010 Durango(Bizkaia)Spain AnyCasting Software co. Ltd.SeoulS.Korea

自从1931年Esser和Lautenbusch第一次在铸铁文献中谈及热分析以来,铸铁的热分析技术已经走过了漫长的道路。目前,热分析已被广泛应用于各种铸铁的过程控制。文中综述了热分析在设备和分析方法方面的最新进展,正是这些技术进步使得热分... 详细信息

自从1931年Esser和Lautenbusch第一次在铸铁文献中谈及热分析以来,铸铁的热分析技术已经走过了漫长的道路。目前,热分析已被广泛应用于各种铸铁的过程控制。文中综述了热分析在设备和分析方法方面的最新进展,正是这些技术进步使得热分析得以成功应用于评估化学成分、石墨化潜能、石墨形状和数量等。详细分析了热分析技术在预测收缩缺陷倾向方面的潜力和局限性,讨论了预测力学性能和收缩倾向的实例。为了让读者更好地了解各种预测的准确性,文中给出了一些数据和图表。

关键词：热分析铸铁熔炼预测

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创新,汽巴视康擦亮另一双眼睛

co.公司 2004年第8期 54-55页

作者： Charles A O'reilly III michael L.Tushman 蔡覃

领导者需要有两双眼睛,一双眼睛盯紧改进提高现有业务,另一双眼睛寻求创新突破。

关键词：汽巴视康公司美国隐形眼镜眼部护理产品创新市场份额企业文化激励机制销售业绩

Assessment of the Evolution and Socio-Economic Impacts of Extreme rainfall Events in October 2019 over the East Africa

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Atmospheric and Climate Sciences 2020年第3期10卷 319-338页

作者： Ladislaus Benedict Chang’a Agnes Lawrence Kijazi Kantamla Biseke Mafuru Alfred Lawrence Kondowe Sarah Emirald Osima Habiba Ismail Mtongori Hashim Karim Ng’ongolo Omari Hamisi Juma Edward michael Tanzania Meteorological Authority Dar es Salaam Tanzania

This study aimed at establishing and quantifying the evolution and socio-economic impacts of extreme rainfall events in October 2019. The study also focused on ascertaining the extent to which the Indian Ocean Dipole (IOD) and the El Ni?o Southern Oscillation (ENSO) influenced anomalous rainfall over East Africa (EA) in October 2019. It employed Singular Value Decomposition (SVD) methods to analyze inter-annual variability of EA rainfall and the Sea Surface Temperature Anomalies (SSTA) over the Indian and Pacific Ocean with a focus on October to December 2019 rainfall season. The SVD analysis enabled the exploration of the leading modes from the mean monthly rainfall and SSTs leading to the determination of the likely influence of the IOD and ENSO respectively. The first SVD coupled modes, which dominate the co-variability between the October rainfall over the EA domain, and SSTA over the Indian and Pacific Oceans based on 1981 to 2010 climatology indicate the monopole positive co-variability with rainfall over the entire EA domain. The co.responding spatial pattern for the SSTA over the Indian Ocean (IO) recaptures the positive IOD event while the central equatorial Pacific Ocean (i.e., over Ni?o 3.4 region) reveals a monopole positive loading, a typical signal for the warm phase of ENSO. The positive rainfall anomaly over the EA during October is found to be associated with either the IOD event or ENSO condition events independently or in phase. However, the inter-annual variability between October rainfall over EA and ENSO reveals a moderate relationship (r = 0.4212) while a robust association (r = 0.7084) is revealed with IOD. comparatively, the October 2019 rainfall anomaly peaks the highest in history over the EA and was found to be coupled with highest positive IOD event in record. Unlikely, the 1997 October rainfall (which peaked the second in history), was associated with the

关键词： Indian Ocean Dipole (IOD) ENSO Sea Surface Temperature Extreme Events OND

Overexpression of the Six1 Homeobox Gene Is Associated with Diffuse Peritoneal Spread and Larger residual Disease after Maximal Cytoreductive Effort in Advanced Ovarian Cancer

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Journal of Cancer Therapy（癌症治疗） 2015年第14期

作者： Julia r. Embry-Schubert Lubna Qamar Monique Spillman michael G. Kelly Susan A. Davidson Kian Behbakht Department of Obstetrics and Gynecology Kaiser-Permanente Denver co USA Division of Gynecologic Oncology Department of Obstetrics and Gynecology University of colorado School of Medicine and Anschutz Medical Campus Aurora co USA Texas Oncology Sammons Cancer Center Baylor University Medical Center Dallas TX USA Wake Forest University School of Medicine One Medical Center Boulevard Winston-Salem NC USA Denver Health Medical Center Denver co USA

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在线检测与分析快速发现不可见的电学缺陷

集成电路应用 2007年第11期24卷 42-44页

作者： michael Schmidt Hyoung Kang Larry Dworkin Kenneth Harris Sherry Lee FEI co. PDF Solutions

在Fab里,在经过特别设计的短流程测试晶圆上进行的快速电学测试和自动FIB/SEM缺陷分析可以将不可见缺陷探测与分析的时间降低一个数量级,并且对不同的设计参数非常敏感,因而有足够的样本来做重要的统计评估。

关键词：缺陷探测在线检测电学设计参数缺陷分析统计评估 Fab 数量级

Plaque increase may be an important contributor to late restenosis after percutaneous transluminal coronary balloon angioplasty

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Chinese Medical Journal 1997年第12期 43-43页

作者： Fengqi Liu, Junbo Ge, Dietrich Baumgart, Günter Grge, michael Haude and raimund Erbel

There is considerable controversy over the mechanism of restenosis after percutaneous balloon angioplasty (PTCA). Vessel remodeling and plaque increase are among the possible contributors but angiography is methodologically limited since it can not differentiate these different mechanisms. For evaluating the contribution of vessel and plaque changes after PTCA, we analyzed serial intravascular ultrasound (IVUS) studies in 59 lesions. IVUS study (3.5 F, 20 MHz catheter, Boston Scientific co,; Hewlett Packard console) was performed immediately after PTCA (POST), and at follow up (FU, 6±1 months). At follow up, 40 lesions (Group Ⅰ) did not show restenosis and 19 (32.2%) lesions (Group Ⅱ) developed restenosis (area Department of Cardiology, University of Essen, Essen, Germany (Liu FQ, Ge JB, Baumgart D, Grge G, Haude M and Erbel r) stenosis >50%). Cross sectional vessel area (VA, mm 2), plaque area (PA, mm 2), and percent area stenosis (A%) were measured. [BHDFG1*2,WK3,WK5,WK11,WK11W]GrOUP Ⅰ (±s)GrOUP Ⅱ (±s) [BHDZ,WK3ZQ,WK5ZQ,WK11,WK11ZQ2W]VAPOST18.1±4.919.1±5.6FU17.7±4.618.9±6.2PAPOST9.9±3.212.0±4.7 *FU10.2±3.415.4±5.0 * A%POST55.6±7.568.4±6.3 *FU58.4±8.781.6±3.4 * * P roup I vs Group II. In summary, plaque increase contributed significantly to late restenosis. This may be partly due to “recovery” of the plaque from redistribution (induced by balloon compression) and/or partly due to intimal proliferation. Greater residual plaque burden was also related to higher possibility of rstenosis.

关键词： Plaque increase may be an important contributor to late restenosis after percutaneous transluminal coronary balloon angioplasty