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Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia

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Horticulture Research 2015年第1期2卷 154-165页

作者： Chao ma Hong Wang Andrew J macnish Alejandro C Estrada-Melo Jing Lin Youhong Chang michael S Reid Cai-Zhong Jiang Department of Plant Sciences University of CaliforniaDavisDavisCA 95616USA Institute of Horticulture Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjing 210014China United States Department of Agriculture Crops Pathology and Genetic Research UnitAgricultural Research ServiceDavisCA 95616USA

The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to ***-sequencing technology permitted us to analyze the transcriptome of *** during both dehydration and *** identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments *** 295 transcription factors(TFs)and 484 protein kinases(PKs)were up-or down-regulated in response to desiccation *** these,the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during *** regulators transduce signal cascades of molecular pathways,including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative *** systems are up-regulated,and the photosynthetic system is modified to reduce ROS *** metabolism may participate in the desiccation tolerance of *** as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate ***-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to *** rehydration,the transcriptome is also enriched in transcripts of genes encoding TFs and PKs,as well as genes involved in photosynthesis,and protein *** data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in ***.

关键词： hydration Antioxidant metabolism

Timanide(Ediacaran–Early Cambrian) Metamorphism at the Transition from Eclogite to Amphibolite Facies in the Beloretsk Complex,SW-Urals,Russia

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Journal of Earth Science 2019年第6期30卷 1144-1165页

作者： Arne P.Willner michael Gopon Johannes Glodny Victor N.Puchkov Hans-Peter Schertl Institute of Geology Mineralogy and GeophysicsRuhr-University BochumD-44780 BochumGermany College of Earth Science and Engineering Shandong University of Science and TechnologyQingdao 266590China Deutsches Geoforschungszentrum GFZ、Telegrafenberg 14473 Potsdam、Germany Institute of Geology and Geochemistry Uralian Branch of Russian Academy of Science、620016 EkaterinburgRussia

The Beloretsk Metamorphic Complex in the SW Urals formed at a convergent eastern margin of Baltica during the Neoproterozoic–Early Cambrian Timanide *** comprises three major units with lenses of facies-critical metabasites within metasedimentary rocks:A lowermost eclogite unit,an intermediate garnet amphibolite unit and an upper amphibolite-greenschist ***(P)-temperature(T)-paths of four rocks from the two lowermost units were determined mainly by PT pseudosection techniques showing similar clockwise loops at different peak metamorphic,watersaturated conditions:A phengite-bearing eclogite shows peak PT conditions of 16.5–18.5 kbar/525–550℃(stage I)followed by stage II at 11.5–13.0 kbar/585–615℃.A garnet amphibolite from the intermediate unit yields lower peak conditions of 11.7–14.5 kbar/480–510℃(stage I)followed by stage II at 9.5–11.0 kbar/535–560℃.However,a granite gneiss in the eclogite unit shows similar maximum pressures as the eclogite,but higher temperatures at 15.6–16.2 kbar/660–675℃,whereas a garnet micaschist contains comparable high pressure relicts,but underwent an advanced midcrustal reequilibration at 7.5–9.0 kbar/555–610℃.We dated the eclogite by a 7-point Rb/Sr mineral isochron(phengite,omphacite,apatite)at 532.2±9.1 ma interpreted as age of crystallisation of the eclogitic peak PT *** age is the youngest compared to the known Timanide metamorphic and magmatic ages.

关键词： Urals eclogite high pressure metamorphism Timanide orogeny Rb-Sr dating PT pseudosections PT-path

四川盆地晚三叠世恐龙足迹化石显示中国最早的恐龙生存环境

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四川盆地晚三叠世恐龙足迹化石显示中国最早的恐龙生存环境

Do ankle braces provide similar effects on ankle biomechanical variables in subjects with and without chronic ankle instability during landing?

第十七届全国古地理学及沉积学学术会议

作者：彭深远刘建 michael J.Benton 金鑫时志强成都理工大学沉积地质研究院成都理工大学油气藏地质及开发工程国家重点实验室成都自然历史博物馆成都理工大学博物馆 School of Earth Sciences University of Bristol

恐龙最早出现的年代、地理起源及早期演化一直是地质与古生物学界关注和研究的热点。最新研究认为恐龙生物群的多样性演化发生于卡尼期(冈瓦纳大陆南部)。20世纪80年代和本世纪初,在华南地区报道了我国最早的恐龙化石记录—晚三叠世须... 详细信息

恐龙最早出现的年代、地理起源及早期演化一直是地质与古生物学界关注和研究的热点。最新研究认为恐龙生物群的多样性演化发生于卡尼期(冈瓦纳大陆南部)。20世纪80年代和本世纪初,在华南地区报道了我国最早的恐龙化石记录—晚三叠世须家河组兽脚类恐龙足迹化石,但出现的地质年代一直未有细致的研究涉及。我们的研究首次报道了成都理工大学博物馆馆藏的在上三叠统须家河组中发现的大量恐龙足迹,其采自雅安天全地区须家河组三段,为以铸模的形式保存在砂岩底面上的兽脚类恐龙足迹,共22只。对天全恐龙足迹进行的足迹遗迹分类学、碎屑锆石年代学和中国恐龙早期迁徙演化过程的研究显示,天全恐龙足迹长为9.8～17.1 cm,为中小型足迹,依据足迹形态和关键特征,认为多数天全恐龙足迹与彭县足迹和卡岩塔足迹特征相似,将其划分为cf. Pengxianpus isp.和cf. Kayentapus isp.,少量小型足迹可能属于跷脚龙-安琪龙-实雷龙足迹群,天全恐龙足迹的造迹者可能为Liliensternus(理理恩龙)或其近亲。恐龙足迹采集层位附近碎屑锆石测年结果显示,最年轻的一组碎屑锆石的加权平均年龄为218.4±4.7 ma,指示中国最早的恐龙足迹可能出现自中诺利期。产足迹化石层位岩石学、沉积学特征显示恐龙生存在高等植物发育的三角洲环境。结合生物热生理学特征及同时期西特提斯地区报道的相关恐龙化石,研究认为晚三叠世位于东特提斯(中国华南)地区的兽脚类恐龙应迁徙自相近纬度的西特提斯地区。

关键词：晚三叠世恐龙足迹锆石U-Pb测年遗迹分类学迁徙演化

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Journal of Sport and Health Science 2012年第2期1卷 114-120页

作者： Songning Zhang michael Wortley Julia Freedman Silvernail Daniel Carson maxime R.Paquette Biomechanics/Sports Medicine Laboratory Department of KinesiologyRecreation and Sport StudiesThe University of TennesseeKnoxvilleTN 37934.USA Pellissippi State Community College KnoxvilleTN 37933USA Department of Kinesiology University of MassachusettsAmherstMA 01003USA Biomechanics/Sports Medicine Laboratory Department of KinesiologyRecreation and Sport StudiesThe University of TennesseeKnoxvilleTN 37934USA

Purpose:The purpose of this study was to examine effects of a sport version of a semi-rigid ankle brace(ElementTM) and a soft ankle brace （ASO） on ankle biomechanics and ground reaction forces（GRFs） during a drop landing activity in subjects with chronic ankle instability(CAD compared to healthy subjects with no history of CAI. Methods:Ten healthy subjects and 10 subjects who had multiple ankle sprains participated in the study as the control and unstable subjects, *** CAI subjects were age,body mass index and gender matched with the control *** arch index and ankle functions of the subjects were measured in a subject screening *** the biomechanical test session,participants performed five trials of drop landing from 0.6 m,wearing no brace（NB）.Element? brace and ASO *** recording of three-dimensional kinematic（240 Hz） and GRF（1200 Hz） data were performed. Results:The CAI subjects had lower ankle functional survey *** arch index and deformity results showed greater arch deformity of ElementTM against a static load than in NB and ASO due to greater initial arch position held by the *** participants had greater eversion velocity than healthy *** ASO brace reduced the first peak vertical GRF whereas ElementTM increased 2nd peak vertical GRF. ElementTMbrace reduced eversion range of motion（ROM） and peak eversion velocity compared to NB and *** addition,ElementTM reduced dorsiflexion ROM and increased peak plantarflexion moment compared to NB and ASO. Conclusion:Results of static arch measurements and dynamic ankle motion suggest that the restrictions offered by both braces are in part due to more dorsiflexed ankle positions at contact,and higher initial arch position and stiffer ankle for ElementTM.

关键词： Drop landing Lateral ankle sprain Recurrent ankle

Functional Surfactants for Molecular Fishing,Capsule Creation,and Single-Cell Gene Expression

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Nano-Micro Letters 2021年第9期13卷 377-385页

作者： Mohammad Suman Chowdhury Xingcai Zhang Leila Amini Pradip Dey Abhishek Kumar Singh Abbas Faghani michael Schmueck‑Henneresse Rainer Haag Institut Für Chemie Und Biochemie Freie Universität BerlinTakustrasse 314195 BerlinGermany John A.Paulson School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA School of Engineering Massachusetts Institute of TechnologyCambridgeMA 02139USA Berlin Institute of Health-Center for Regenerative Therapies CharitéUniversitätsmedizin Berlin-CVKFöhrer Str.1513353 BerlinGermany BerlinCenter for Advanced Therapies CharitéUniversitätsmedizin Berlin-CVKFöhrer Str.1513353 BerlinGermany

Creating a single surfactant that is open to manipulation,while maintaining its surface activity,robustness,and compatibility,to expand the landscape of surfactant-dependent assays is extremely *** report an oxidation-responsive precursor with thioethers and multiple 1,2-diols for creating a variety of functional surfactants from one parent *** these multifunctional surfactants,we stabilize microfluidics-generated aqueous *** droplets encapsulate different components and immerse in a bioinert oil with distinct interfaces where an azide-bearing surfactant allow fishing of biomolecules from the droplets,aldehyde-bearing surfactant allow fabrication of microcapsules,and hydroxyl-bearing surfactants,with/without oxidized thioethers,allow monitoring of single-cell gene *** multifunctional surfactants poses opportunities for broad applications,including adsorption,bioanalytics,catalysis,formulations,coatings,and programmable subset of emulsions.

关键词： Dendronized fluorosurfactants Droplet microfluidics Microcapsules Oxidation-responsive fluorosurfactants Cell encapsulation

Chloramination of wastewater effluent: Toxicity and formation of disinfection byproducts

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Journal of Environmental Sciences 2017年第8期29卷 135-145页

作者： Julien Le Roux michael J.Plewa Elizabeth D.Wagner maolida Nihemaiti Azra Dad Jean-Philippe Croué LEESU(UMR ma 102) Université Paris-Est–AgroParisTech Department of Crop Sciences the Global Safe Water InstituteUniversity of Illinois at Urbana-Champaign Curtin Water Quality Research Centre Department of ChemistryCurtin University US Food and Drug Administration National Center for Toxicological Research

The reclamation and disinfection of waters impacted by human activities（e.g., wastewater effluent discharges） are of growing interest for various applications but has been associated with the formation of toxic nitrogenous disinfection byproducts（N-DBPs）. Monochloramine used as an alternative disinfectant to chlorine can be an additional source of nitrogen in the formation of N-DBPs. Individual toxicity assays have been performed on many DBPs, but few studies have been conducted with complex mixtures such as wastewater effluents. In this work, we compared the cytotoxicity and genotoxicity of wastewater effluent organic matter（Ef OM） before and after chloramination. The toxicity of chloraminated Ef OM was significantly higher than the toxicity of raw Ef OM, and the more hydrophobic fraction（HPO）isolated on XAD-8 resin was more toxic than the fraction isolated on XAD-4 *** DBPs were also isolated on the XAD-8 resin. N-DBPs（i.e., haloacetonitriles or haloacetamides） were responsible for the majority of the cytotoxicity estimated from DBP concentrations measured in the XAD-8 and XAD-4 fractions（99.4% and 78.5%, respectively）.Measured DBPs accounted for minor proportions of total brominated and chlorinated products, which means that many unknown halogenated compounds were formed and can be responsible for a significant part of the toxicity. Other non-halogenated byproducts（e.g.,nitrosamines） may contribute to the toxicity of chloraminated effluents as well.

关键词： Disinfection byproducts Chloramination Toxicity Wastewater XAD resins Haloacetonitriles Haloacetamides

扬子板块西北缘碧口地块中-新生代变质、变形与年代限定

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地学前缘 2018年第1期25卷 80-94页

作者：许晨光颜丹平古术航孟祥坤邱亮 michael L.WELLS 王继斌马芳中国地质大学(北京)地球科学与资源学院北京100083 Department of Geoscience University of NevadaLas VegasNevadaUSA 北京大学地球与空间科学学院造山带与地壳演化教育部重点实验室北京100871

碧口地块位于扬子板块西北缘,由北部变质沉积岩系及南部变质中基性火山岩组成。碧口地块北部岩石变质为千枚岩、云母石英片岩,南部岩石经历绿片岩-蓝片岩相变质。由于对碧口地块变形特征及区域变质作用分析深度不够,缺少精确的变质时间... 详细信息

碧口地块位于扬子板块西北缘,由北部变质沉积岩系及南部变质中基性火山岩组成。碧口地块北部岩石变质为千枚岩、云母石英片岩,南部岩石经历绿片岩-蓝片岩相变质。由于对碧口地块变形特征及区域变质作用分析深度不够,缺少精确的变质时间限定,碧口地块变质杂岩系性质、区域变质年代及其大地构造属性始终处于争议之中,导致对西秦岭和龙门山造山过程的认识产生分歧。通过详细区域构造解析,将碧口地块划分为3期变形:D1期以紧闭褶皱、面理S1及矿物线理L1为特征;D2期形成于由北向南的正断拆离作用,显示韧性变形特征,D2期变形改造先期逆断层并形成半地堑盆地;D3期形成于喜山期逆冲走滑运动,以脆性变形为主要特征。本研究获得碧口变质岩中变形脉锆石U-Pb年龄(227.2±6.2)ma,碧口地块已报道的D1期变形后期侵入体年龄为226~215 ma,从而限定D1期碧口区域变质变形年龄为约220 ma。因此推测D1期形成于三叠纪末华南、华北板块碰撞,碧口地块区域变质主要形成于这一时期的俯冲碰撞作用;D2与D3期则与青藏高原的东向扩张有关。

关键词：碧口地块区域变质扬子板块三叠纪

The Tomato Hybrid Proline-rich Protein regulates the abscission zone competence to respond to ethylene signals

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Horticulture Research 2018年第1期5卷 528-544页

作者： Srivignesh Sundaresan Sonia Philosoph-Hadas Chao ma Cai-Zhong Jiang Joseph Riov Raja Mugasimangalam Betina Kochanek Shoshana Salim michael SReid Shimon Meir Department of Postharvest Science of Fresh Produce Agricultural Research Organization(ARO)The Volcani CenterRishon LeZiyonIsrael The Robert H.Smith Institute of Plant Sciences and Genetics in Agriculture The Robert H.Smith Faculty of AgricultureFood and EnvironmentThe Hebrew University of JerusalemRehovotIsrael Department of Plant Sciences University of CaliforniaDavisCAUSA Crops Pathology&Genetic Research Unit USDA-ARSDavisCAUSA Department of Bioinformatics QTLomics Technologies Pvt.LtdBangaloreIndia Department of Nano Science and Technology Tamil Nadu Agricultural UniversityCoimbatoreIndia Beijing Key Laboratory of Development and Quality Control of Ornamental Crops Department of Ornamental HorticultureChina Agricultural UniversityBeijingChina

The Tomato Hybrid Proline-rich Protein(THyPRP)gene was specifically expressed in the tomato(Solanum lycopersicum)flower abscission zone(FAZ),and its stable antisense silencing under the control of an abscission zone(AZ)-specific promoter,Tomato Abscission Polygalacturonase4,significantly inhibited tomato pedicel abscission following flower *** understanding the THyPRP role in regulating pedicel abscission,a transcriptomic analysis of the FAZ of THyPRP-silenced plants was performed,using a newly developed AZ-specific tomato microarray *** expression of THyPRP in the silenced plants was already observed before abscission induction,resulting in FAZ-specific altered gene expression of transcription factors,epigenetic modifiers,post-translational regulators,and *** data demonstrate that the effect of THyPRP silencing on pedicel abscission was not mediated by its effect on auxin balance,but by decreased ethylene biosynthesis and ***,THyPRP silencing revealed new players,which were demonstrated for the first time to be involved in regulating pedicel abscission *** include:gibberellin perception,Ca2+-Calmodulin signaling,Serpins and Small Ubiquitin-related Modifier proteins involved in post-translational modifications,Synthaxin and SNARE-like proteins,which participate in exocytosis,a process necessary for cell *** changes,occurring in the silenced plants early after flower removal,inhibited and/or delayed the acquisition of the competence of the FAZ cells to respond to ethylene *** results suggest that THyPRP acts as a master regulator of flower abscission in tomato,predominantly by playing a role in the regulation of the FAZ cell competence to respond to ethylene signals.

关键词： Hybrid inhibited competence

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多级接触氧化工艺处理汽车涂装废水工程实例

水处理技术 2018年第5期44卷 135-139页

作者：朱玉芳吕力婷朱彤 michael Groetzbach 马永光韩辉东北大学机械工程与自动化学院 110004 华晨宝马汽车有限公司 110143 辽宁省机械研究院有限公司辽宁沈阳110032

针对汽车厂涂装废水的水质特征和处理工艺存在的问题,采用多级接触氧化系统对原有的物化处理工艺进行升级改造,进一步降低涂装废水的有机污染物含量。结果表明,最终出水COD可保持在200 mg/L以下,水质指标均可完全达到DB 21/1627-2008排... 详细信息

针对汽车厂涂装废水的水质特征和处理工艺存在的问题,采用多级接触氧化系统对原有的物化处理工艺进行升级改造,进一步降低涂装废水的有机污染物含量。结果表明,最终出水COD可保持在200 mg/L以下,水质指标均可完全达到DB 21/1627-2008排放要求。整个多级接触氧化系统处理废水能力为480 m^3/d,平均处理费用为1.96元/t废水。该工程的成功应用和经验可供相关行业的废水处理参考。

关键词：汽车涂装废水多级接触氧化工程实例