检索结果-南通市图书馆

Evaluation of Real-Time 16S rDNA PCR and Pyrosequencing for Routine Identification of Bacteria in joint Fluid and Tissue Specimens

在线全文

学校读者我要写书评

暂无评论

Open journal of Medical Microbiology 2011年第1期1卷 1-6页

作者： Naomi j. Gadsby Alev Onen Sally-Anne phillips Luke Tysall Steffen j. Breusch Hamish Simpson jayshree Dave Elzbieta Czarniak Kate E. Templeton Microbiology Department of Laboratory Medicine Royal Infirmary of Edinburgh Department Orthopaedic Surgery Royal Infirmary of Edinburgh

16S rDNA PCR and sequencing are powerful tools for bacterial detection and identification, although their routine use is not currently widespread in the field of clinical microbiology. The availability of pyrosequencing now makes 16S rDNA assays more accessible to routine diagnostic laboratories, but this approach has had limited evaluation in general diagnostic practice. In this study we evaluated a real-time 16S rDNA PCR and pyrosequencing assay for use in a routine microbiology laboratory, by retrospectively testing joint fluid and joint tissue specimens received for conventional culture. We found that use of the real-time 16S rDNA assay was clinically valuable in this specimen type because it enabled us to identify a small number of culture-negative infections. Although faster and less labour-intensive, we found that the utility of pyrosequencing for pathogen identification is still hampered by shorter read lengths compared to conventional (Sanger) sequencing. Combining results from both molecular and conventional culture methods, bacteria were only detected in 11.8% specimens in this study. However, the detection rate was increased to 18.6% if specimens were only included from patients with a documented clinical suspicion of infection. In conclusion, while pyrosequencing had significant advantages in speed and ease-of-use over conventional sequencing, multiple reactions will be required to deliver comparable species-level identification, thus negating many of the benefits of using the technique. We found that 16S rDNA PCR and sequencing should be rationally targeted on the basis of good clinical information in the routine diagnostic setting, and not used as a general screening test for the exclusion of bacterial infection in joint specimens.

关键词： 16S rRNA Real-Time PCR Sequencing Pyrosequencing Orthopaedic Infection

The effectiveness of shared clinical teaching in nursing

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International journal of Nursing Sciences 2019年第2期6卷 211-215页

作者： Karen F.phillips Lizy Mathew Nadine Aktan Priyadharshiny Sandanapitchai Department of Nursing College of Science and HealthWilliam Paterson University300 Pompton RdWayneNJ07470USA Department of Psychology College of Humanities and ScienceWilliam Paterson University300 Pompton RdWayneNJ07470USA

Aim: Clinical learning is a critical component of a nursing *** satisfaction in clinical environment is crucial to foster a positive learning *** shortages have made clinical teaching more challenging;as such,alternate models of clinical teaching must be explored by nursing *** purpose of this study was to measure the perception of student satisfaction in regard tothe effectiveness of shared clinical teaching in ***: Utilizing Chan's Clinical Learning Environment Inventory (CLEI),this quantitative comparative study examined nursing students' preferred and actual clinical learning *** CLEI contains 42 questions in six subscales: Individualization,Innovation,Involvement,Personalization,Task Orientation,and Satisfaction in both the actual and preferred clinical learning *** sample consisted of 202 nursing students in two groups: the first group had 91 students who experienced shared clinical teaching with two faculty,whereas the second group had 111 students who experienced the traditional,single faculty *** results were analyzed using independent sample ***: The preferred learning environment was rated highest in all six *** of the Satisfaction subscale and the Innovation subscale for Actual Learning Environment,and the score of the Innovation subscale for Preferred Learning Environment of students experiencing shared clinical teaching with two faculty were higher than the scores of students experiencing traditional,single faculty model,with statistically significance (P< 0.05).Conclusion: The results indicated students preferred the shared clinical teaching model with two faculty over the single faculty *** programs can utilize this model and apply these results to develop and maintain quality clinical teaching.

关键词： Clinical Clerkship Clinical learning environment Faculty shortage Nursing education Students Nursing Questionnaires

在线全文

学校读者我要写书评

暂无评论

全球肉鸡生产福利研究现状与展望

中国家禽 2011年第16期33卷 4-9页

作者： A.Robins Cj.C.phillips 林又米(译) 澳大利亚昆士兰大学不详

本文从全球肉鸡产业的角度对主产国的肉鸡福利问题和相关措施进行了比较与分析。全球肉鸡产业中,美国、澳大利亚等国的肉鸡产业主要由少数几个综合性大型企业垄断,欧洲的肉鸡产业更加多样化,竞争更激烈,而且政府的监管力度更强。与美国... 详细信息

本文从全球肉鸡产业的角度对主产国的肉鸡福利问题和相关措施进行了比较与分析。全球肉鸡产业中,美国、澳大利亚等国的肉鸡产业主要由少数几个综合性大型企业垄断,欧洲的肉鸡产业更加多样化,竞争更激烈,而且政府的监管力度更强。与美国相比,欧洲关于肉鸡福利的研究与规定更多,尤其是英国。两个主要的肉鸡生产大国巴西和中国在福利条例方面努力甚微。一些福利问题是区域性的,如速生型肉鸡从封闭式鸡舍迁至散养环境下遭遇的高温问题。然而,困扰集约化舍养肉鸡的福利问题基本相同,如速生型肉鸡的心脏和腿部问题;高密度饲养空间下鸡难以移动和伸展,易感染接触性皮炎。与其他地区相比,欧洲的肉鸡福利研究更直接针对肉鸡的行为和认知需求,有助于帮助肉鸡产业更好地满足未来消费者对福利的要求。

关键词：肉鸡福利行为法规

Phylogenetic relationships and morphological reappraisal of Melanommataceae(Pleosporales)

在线全文

学校读者我要写书评

暂无评论

Fungal Diversity 2015年第5期74卷 267-324页

作者： Qing Tian jian Kui Liu Kevin D.Hyde Dhanushka N.Wanasinghe Saranyaphat Boonmee Subashini C.jayasiri Zong Long Luo joanne E.Taylor Alan j.L.phillips Darbhe j.Bhat Wen jing Li Hiran Ariyawansa Kasun M.Thambugala EBGareth jones Putarak Chomnunti Ali H.Bahkali jian Chu Xu Erio Camporesi Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanPeople’s Republic of China World Agroforestry Centre East and Central AsiaKunming 650201YunnanPeople’s Republic of China Center for Mountain Ecosystem Studies Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Guizhou Key Laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouChina Royal Botanic Garden Edinburgh 20A Inverleith RowEdinburgh EH35LRUK UCIBIO Faculdade de Ciências e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal Formerly Department of BotanyGoa UniversityGoa-403206India Res:No.128/1–j Azad Housing SocietyCurcaP.O.Goa Velha-403108India Department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box:2455Riyadh 1145Saudi Arabia A.M.B.Gruppo Micologico Forlivese BAntonio Cicognani Via Roma 18ForlìItaly A.M.B.Circolo Micologico BGiovanni Carini C.P.314 BresciaItaly Societàper gli Studi Naturalistici della Romagna C.P.144 BagnacavalloRAItaly

The family Melanommataceae is widespread in temperate and subtropical regions and species invariably occur on twigs or bark of various woody plants in terrestrial,marine or freshwater *** this paper,the type species of 26 genera of the family are re-described and illustrated.A multi-gene phylogeny based on maximum likelihood and Bayesian analyses of LSU,SSU,RPB2 and EF-1αsequence data of species of Melanommataceae is *** new genera,Muriformistrickeria,Pseudostrickeria and Thysanolaenae are *** is synonymized under ***,Astrosphaeriella,Beverwykella,Caryosporella,Sporidesmiella and Pseudotrichia are excluded from Melanommataceae based on molecular phylogenetic ***,20 genera are accepted in *** on the phylogenetic data,five new species,Byssosphaeria siamensis,Herpotrichia vaginatispora,Pseudostrickeria muriformis,Pseudostrickeria ononidis and Muriformistrickeria rubi,are introduced.

关键词： Byssosphaeria Herpotrichia Multi-gene Phylogeny Muriformistrickeria New species Pseudostrickeria Taxonomy Thysanolaenae Type

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

单眼视减缓了青少年单侧近视的进展

世界核心医学期刊文摘（眼科学分册） 2005年第12期1卷 40-41页

作者： phillips j.R 王海燕 Department of Optometry and Vision Science University of Auckland Private Bag 92019 Auckland New Zealand.Dr

Aim: To evaluate the acceptability, effectivity, and side effects of amonovision spectacle correction designed to reduce accommodation and myopia progression in schoolchildren. Methods: Dominant eyes of 11 year old children with myopia (- 1.00 to - 3.00 D mean spherical equivalent)were corrected for distance; fellow eyes were uncorrected or corrected to keep the refractive imbalance juvenile myopia. However, myopia progression was significantly reduced in the near corrected eye, suggesting that sustained myopic defocus slows axial elongation of the human eye.

关键词：视力矫正光学离焦睫状肌麻痹等量球镜眼轴自动验光仪对侧眼矫正镜片玻璃体腔时用

FOXP3在炎症性肠病患者外周血和肠黏膜中的表达特点

在线全文

学校读者我要写书评

暂无评论

胃肠病学 2007年第2期12卷 92-95页

作者：温忠慧 Gail West Manijeh H phillips Claudio Fiocchi 四川大学华西医院消化内科 610041 Division of Gastroenterology University Hospitals of ClevelandCase Western Reserve University

背景:炎症性肠病(IBD)的发病与一系列免疫异常有关。免疫反应受特定的免疫调节细胞调控,包括CD4+CD25+T细胞,而CD4+CD25+T细胞的发育和功能可能受FOXP3基因调控。目的:研究FOXP3的表达特点以及IBD时FOXP3表达的改变。方法:以免疫磁珠分... 详细信息

背景:炎症性肠病(IBD)的发病与一系列免疫异常有关。免疫反应受特定的免疫调节细胞调控,包括CD4+CD25+T细胞,而CD4+CD25+T细胞的发育和功能可能受FOXP3基因调控。目的:研究FOXP3的表达特点以及IBD时FOXP3表达的改变。方法:以免疫磁珠分离CD4+、CD4+CD25+和CD4+CD25-T细胞。以逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法检测IBD患者和非IBD对照外周血单个核细胞(PBMC)、肠固有层单个核细胞(LPMC)和肠黏膜组织中FOXP3的表达。结果:CD4+CD25+T细胞中FOXP3mRNA的表达明显强于CD4+T细胞和未分离的PBMC或LPMC。T细胞受体(TCR)激活后,或经白细胞介素(IL)-2、IL-15作用后,PBMC或LPMCFOXP3mRNA或蛋白表达明显增强。12例IBD中9例(75.0%)肠黏膜LPMC中检测到FOXP3mRNA表达,高于对照肠黏膜的47.1%(8/17,P>0.05)。未受刺激的IBD和对照肠黏膜组织中未检测到FOXP3mRNA表达。结论:人类FOXP3的表达受T细胞活化影响。IBD患者肠黏膜LPMC中FOXP3mRNA的表达率有增高的趋势,但与对照肠黏膜相比无显著差异。

关键词： FOXP3 炎性肠疾病 CD4阳性T淋巴细胞淋巴细胞活化

在线全文

学校读者我要写书评

暂无评论

社区人群精神障碍患病率调查方法

中国心理卫生杂志 2010年第3期24卷 191-194页

作者：李献云王志青费立鹏北京回龙观医院北京心理危机研究与干预中心北京100096

精神障碍已成为重要的公共卫生问题。世界卫生组织(WHO)推算,2020年全球精神和行为障碍占全部疾病负担的比例会由1990年的11%升至15%。2004年我国神经精神障碍与自杀占全部疾病负担的20%。因此,掌握精神障碍的患病率对合理配置卫生资源... 详细信息

精神障碍已成为重要的公共卫生问题。世界卫生组织(WHO)推算,2020年全球精神和行为障碍占全部疾病负担的比例会由1990年的11%升至15%。2004年我国神经精神障碍与自杀占全部疾病负担的20%。因此,掌握精神障碍的患病率对合理配置卫生资源、有效防治各类精神障碍至关重要。本文作者与浙江、山东、青海、甘肃4地工作者合作开展了精神障碍流调,[第一段]

关键词：精神障碍现况调查方法学

在线全文

学校读者我要写书评

暂无评论

妇科显微手术

1984年

作者：（美）菲利普斯（j.M.phillips）姚家祥译

本书对妇科手术显微镜、手术器械、基本操作训练、手术前后处理、输卵管和卵巢各咱显微手术的操作步骤和注意事项等进行详细介绍。

ISBN: (数字)14048·4595

ISBN: (纸本)14048·4595

本书对妇科手术显微镜、手术器械、基本操作训练、手术前后处理、输卵管和卵巢各咱显微手术的操作步骤和注意事项等进行详细介绍。

关键词：妇科外科手术显微外科学

来源：汇雅图书（南通市图书馆... 评论

在线全文

汇雅图书（南通市图书馆）

学校读者我要写书评

暂无评论

共轭亚油酸水包油型乳液的物理化学稳定性

农业工程学报 2013年第11期29卷 270-277页

作者：徐琼姚晓琳王娜娜田大志方亚鹏 Glyn O.phillips Katsuyoshi Nishinari 湖北工业大学轻工学部食品与制药工程学院菲利普斯亲水胶体研究中心武汉430068

共轭亚油酸(conjugated linoleic acid,CLA)是一种具有多重生理功效的不饱和脂肪酸,其氧化稳定性较差,对光、热、氧气很不稳定。以亲水胶体为乳化剂制备CLA的水包油(O/W)型乳液可改善其氧化稳定性,扩大其在食品中的应用。该研究采用改... 详细信息

共轭亚油酸(conjugated linoleic acid,CLA)是一种具有多重生理功效的不饱和脂肪酸,其氧化稳定性较差,对光、热、氧气很不稳定。以亲水胶体为乳化剂制备CLA的水包油(O/W)型乳液可改善其氧化稳定性,扩大其在食品中的应用。该研究采用改性阿拉伯胶EM2为乳化剂、2种不同黏度的CLA为油相,通过测定乳液颗粒的粒径和粒径分布以及乳液在40℃贮存过程中的过氧化值和茴香胺值,研究了CLA的水包油(O/W)型乳液的物理化学稳定性。结果表明,高质量分数的EM2有利于形成粒径更小且分布均一的乳液颗粒。乳液的氧化稳定性很大程度上依赖于其物理稳定性。对于黏度较小的CLA,在各测试EM2质量分数下,CLA乳液具有较好的物理稳定性,且随着EM2质量分数的增大,乳液氧化稳定性提高。对于黏度较大的CLA,EM2质量分数为5%时,乳液具有较好的物理化学稳定性;增大EM2的质量分数,其稳定性下降。该研究可为今后研究基于乳液技术的功能性因子保护和增效提供参考,有利于CLA在食品行业中的推广应用。

关键词：脂肪酸乳液物理稳定性氧化稳定性共轭亚油酸(CLA)