检索结果-南通市图书馆

Molecular Plant 2012年第6期5卷 1281-1294页

作者： M. Asif Arif Isam Fattash Zhaorong Ma Sung Hyun Cho Anna K. Beike Ralf Reski michael j. axtell Wolfgang Frank Plant Biotechnology Faculty of Biology University of Freiburg Schaenzlestral3e 1 D-79104 Freiburg Germany Department of Biology Pennsylvania State University University Park PA 16802 USA FRISYs Freiburg Initiative for Systems Biology D-79104 Freiburg: Germany BIOSS Centre for Biological Signalling Studies D-79104 Freiburg Germany FRIAS Freiburg Institute for Advanced Studies D-79104 Freiburg Germany

Trans-acting small interfering RNAs （ta-siRNAs） are plant-specific siRNAs released from TAS precursor tran- scripts after microRNA-dependent cleavage, conversion into double-stranded RNA, and Dicer-dependent phased process- ing. Like microRNAs （miRNAs）, ta-siRNAs direct site-specific cleavage of target RNAs at sites of extensive complementarity. Here, we show that the DICER-LIKE 4 protein of Physcomitrella patens （PpDCL4） is essential for the biogenesis of 21 nucleotide （nt） ta-siRNAs. In APpDCL4 mutants, off-sized 23 and 24-nt ta-siRNAs accumulated as the result of PpDCL3 activity. APpDCL4 mutants display severe abnormalities throughout Physcomitrella development, including sterility, that were fully reversed in APpDCL3/APpDCL4 double-mutant plants. Therefore, PpDCL3 activity, not loss of PpDCL4 function per se, is the cause of the APpDCL4 phenotypes. Additionally, we describe several new Physcomitrella trans-acting siRNA loci, three of which belong to a new family, TA56. TAS6 loci are typified by sliced miR156 and miR529 target sites and are in close proximity to PpTAS3 loci.

关键词： ta-siRNA miR156 PpDCL3 PpDCL4 development moss.

NECAB family of neuronal calcium-binding proteins in health and disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第5期20卷 1236-1243页

作者： Diones Bueno michael K.E.Schafer Sudena Wang michael j.Schmeisser Axel Methner Institute for Molecular Medicine University Medical Center of the Johannes Gutenberg-UniversityMainzGermany Department of Anesthesiology University Medical Center of the Johannes Gutenberg-UniversityMainzGermany Institute of Anatomy University Medical Center of the Johannes Gutenberg-UniversityMainzGermany

The N-terminal EF-hand calcium-binding proteins 1–3(NECAB1–3) constitute a family of predominantly neuronal proteins characterized by the presence of at least one EF-hand calcium-binding domain and a functionally less well characterized C-terminal antibiotic biosynthesis monooxygenase domain. All three family members were initially discovered due to their interactions with other proteins. NECAB1 associates with synaptotagmin-1, a critical neuronal protein involved in membrane trafficking and synaptic vesicle exocytosis. NECAB2 interacts with predominantly striatal G-protein-coupled receptors, while NECAB3 partners with amyloid-β A4 precursor protein-binding family A members 2 and 3, key regulators of amyloid-β production. This demonstrates the capacity of the family for interactions with various classes of proteins. NECAB proteins exhibit distinct subcellular localizations: NECAB1 is found in the nucleus and cytosol, NECAB2 resides in endosomes and the plasma membrane, and NECAB3 is present in the endoplasmic reticulum and Golgi apparatus. The antibiotic biosynthesis monooxygenase domain, an evolutionarily ancient component, is akin to atypical heme oxygenases in prokaryotes but is not wellcharacterized in vertebrates. Prokaryotic antibiotic biosynthesis monooxygenase domains typically form dimers, suggesting that calcium-mediated conformational changes in NECAB proteins may induce antibiotic biosynthesis monooxygenase domain dimerization, potentially activating some enzymatic properties. However, the substrate for this enzymatic activity remains uncertain. Alternatively, calcium-mediated conformational changes might influence protein interactions or the subcellular localization of NECAB proteins by controlling the availability of protein–protein interaction domains situated between the EF hands and the antibiotic biosynthesis monooxygenase domain. This review summarizes what is known about genomic organization, tissue expression, intracellular localization, interac

关键词： calcium EF hand mitochondria nervous system signal transduction

Knowing your enemies： Integrating molecular and ecological methods to assess the impact of arthropod predators on crop pests

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Insect Science 2015年第1期22卷 6-19页

作者： michael j. Furlong School of Biological Sciences The University of Queensland St Lucia Queensland 4072 Austrafia

The importance of natural enemies as the foundation of integrated pest management （IPM） is widely accepted, but few studies conduct the manipulative field experiments necessary to directly quantify their impact on pest populations in this context. This is particularly true for predators. Studying arthropod predator-prey interactions is inherently difficult： prey items are often completely consumed, individual predator-prey interactions are ephemeral （rendering their detection difficult） and the typically fluid or soft-bodied meals cannot be easily identified visually within predator guts. Serological techniques have long been used in arthropod predator gut-contents analysis, and current enzyme linked immunosorbent assays （ELISA） are highly specific and sensitive. Recently, poly- merase chain reaction （PCR） methods for gut-contents analysis have developed rapidly and they now dominate the diagnostic methods used for gut-contents analysis in field-based research. This work has identified trophic linkages within food webs, determined predator diet breadth and preference, demonstrated the importance of cannibalism and intraguild predation within and between certain taxa, and confirmed the benefits （predator persis- tence） and potential disadvantages （reduced feeding on pest species） of the availability of alternative nonpest prey. Despite considerable efforts to calibrate gut-contents assays, these methods remain qualitative. Available techniques for predator gut-contents analysis can provide rapid, accurate, cost-effective identification of predation events. As such, they perfectly compliment the ecological methods developed to directly assess predator im- pacts on prey populations but which are imperfect at identifying the key predators. These diagnostic methods for gut-contents analysis are underexploited in agricultural research and they are almost never applied in unison with the critical field experiments to measure predator impact. This paper stresses the need fo

关键词： conservation biological control enzyme linked immunosorbent assay (ELISA) gut-contents analysis immunomarking integrated pest management (IPM) polymerase chain reaction (PCR)

A primitive confuciusornithid bird from China and its implications for early avian flight

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Earth Sciences 2008年第5期51卷 625-639页

作者： michael j. BENTON Department of Earth Sciences University of Bristol Bristol BS8 1RJ UK

Confuciusornithids, lived from 120–125 million years ago, form a basal bird group and include the oldest birds with horny beaks. Here we describe Eoconfuciusornis zhengi, gen. et sp. nov. from the Early Cretaceous Dabeigou Formatio (131 Ma) in Fengning, Hebei Province, northern China. It represents a new and, more primitive than other known, member of this group and extends the lifespan of this family to 11 Ma, the longest of any known Early Cretaceous avian lineages. Furthermore, Eoconfuciusornis and its relatives present many osteological transformations, such as the size increase of the deltopectoral crest of the humerus and the keel of the sternum, apparently an adaptation toward improved flight in the evolution of the Confuciusornithidae.

关键词： Birds Early Cretaceous confuciusornithids flight

Reevaluating the conceptual framework for applied research on host-plant resistance

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Insect Science 2013年第3期20卷 263-272页

作者： michael j. Stout Department of Entomology Louisiana State University Agricultural Center 404 Life Sciences Building Baton Rouge LA 70803 USA

Applied research on host-plant resistance to arthropod pests has been guided over the past 60 years by a framework originally developed by Reginald Painter in his 1951 book, Insect Resistance in Crop Plants. Painter divided the ＂phenomena＂ of resistance into three ＂mechanisms,＂ nonpreference （later renamed antixenosis）, antibiosis, and tolerance. The weaknesses of this framework are discussed. In particular, this trichotomous framework does not encompass all known mechanisms of resistance, and the antixenosis and antibiosis categories are ambiguous and inseparable in practice. These features have perhaps led to a simplistic approach to understanding arthropod resistance in crop plants. A dichotomous scheme is proposed as a replacement, with a maj.r division between resistance （plant traits that limit inj.ry to the plant） and tolerance （plant traits that reduce amount of yield loss per unit inj.ry）, and the resistance category subdivided into constitutive/inducible and direct/indirect subcategories. The most important benefits of adopting this dichotomous scheme are to more closely align the basic and applied literatures on plant resistance and to encourage a more mechanistic approach to studying plant resistance in crop plants. A more mechanistic approach will be needed to develop novel approaches for integrating plant resistance into pest management programs.

关键词： antibiosis antixenosis host-plant resistance integrated pest management,plant-insect interactions tolerance

Deciphering Water Oxidation Catalysts: The Dominant Role of Surface Chemistry over Reconstruction Degree in Activity Promotion

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

NANO-MICRO LETTERS 2025年第1期17卷 1-15页

作者： An, Li Li, j.anyi Sun, Yuanmiao Zhu, j.amin Seow, j.stin Zhu Yeow Zhang, Hong Zhang, Nan Xi, Pinxian Xu, Zhichuan j. Yan, Chun-Hua Lanzhou Univ Frontiers Sci Ctr Rare Isotopes Key Lab Nonferrous Met Chem & Resources Utilizat G State Key Lab Appl Organ ChemColl Chem & Chem Eng Lanzhou 730000 Peoples R China Nanyang Technol Univ Sch Mat Sci & Engn Singapore 639798 Singapore Chinese Acad Sci Fac Mat Sci & Energy Engn Shenzhen Inst Adv Technol Inst Technol Carbon Neutral Shenzhen 518055 Peoples R China Yunnan Univ Natl Ctr Int Res Photoelect & Energy Mat Electron Microscopy Ctr Sch Mat & EnergyKey Lab Electromagnet Mat & Devic Kunming 650091 Peoples R China Baotou Res Inst Rare Earths State Key Lab Baiyunobo Rare Earth Resource Res & Baotou 014030 Peoples R China Peking Univ Beijing Natl Lab Mol Sci State Key Lab Rare Earth Mat Chem & Applicat PKU HKU Joint Lab Rare Earth Mat & Bioinorgan Chem Beijing 100871 Peoples R China

Demonstrate that the key factor to determine the activity of reconstructed surfaces is the surface chemistry, instead of reconstruction degree using the popular perovskite LaNi1-xFexO3 oxides as model *** content can influence both the surface reconstruction degree, the activation degree, and the activity of reconstructed *** oxygen evolution reaction activity of reconstructed catalysts is primarily governed by the chemistry of the reconstructed surface species. Water splitting hinges crucially on the availability of electrocatalysts for the oxygen evolution reaction. The surface reconstruction has been widely observed in perovskite catalysts, and the reconstruction degree has been often correlated with the activity enhancement. Here, a systematic study on the roles of Fe substitution in activation of perovskite LaNiO3 is reported. The substituting Fe content influences both current change tendency and surface reconstruction degree. LaNi0.9Fe0.1O3 is found exhibiting a volcano-peak intrinsic activity in both pristine and reconstructed among all substituted perovskites in the LaNi1-xFexO3 (x = 0.00, 0.10, 0.25, 0.50, 0.75, 1.00) series. The reconstructed LaNi0.9Fe0.1O3 shows a higher intrinsic activity than most reported NiFe-based catalysts. Besides, density functional theory calculations reveal that Fe substitution can lower the O 2p level, which thus stabilize lattice oxygen in LaNi0.9Fe0.1O3 and ensure its long-term stability. Furthermore, it is vital interesting that activity of the reconstructed catalysts relied more on the surface chemistry rather than the reconstruction degree. The effect of Fe on the degree of surface reconstruction of the perovskite is decoupled from that on its activity enhancement after surface reconstruction. This finding showcases the importance to customize the surface chemistry of reconstructed catalysts for water oxidation.

关键词： Oxygen evolution reaction Perovskite oxides Doping Activation and reconstruction

Effects and mechanisms of Bacillus thuringiensis crystal toxins for mosquito larvae

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2017年第5期24卷 714-729页

作者： Qi Zhang Gang Hua michael j. Adang College of Plant Protection Shenyang Agricultural University Shenyang China Department of Entomology University of GeorgiaAthens GA USA Department of Biochemistry & Molecular Biology University of Georgia Athens GA USA

Bacillus thuringiensis is a Gram-positive aerobic bacterium that produces insecticidal crystalline inclusions during sporulation phases of the mother cell. The vir- ulence factor, known as parasporal crystals, is composed of Cry and Cyt toxins. Most Cry toxins display a common 3-domain topology. Cry toxins exert intoxication through toxin activation, receptor binding and pore formation in a suitable larval gut environment. The mosquitocidal toxins of Bt subsp, israelensis （Bti） were found to be highly active against mosquito larvae and are widely used for vector control. Bt subsp, j.gathesan is another strain which possesses high potency against broad range of mosquito larvae. The present review summarizes characterized receptors for Cry toxins in mosquito larvae, and will also discuss the diversity and effects of 3-D mosquitocidal Cry toxin and the ongo- ing research for Cry toxin mechanisms generated from investigations of lepidopteran and dipteran larvae.

关键词： Bacillus thuringiensis Cry toxins mode of action mosquito receptors

Effects of single and dual species herbivory on the behavioral responses of three thrips species to cotton seedlings

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2017年第4期24卷 684-698页

作者： Rehan Silva Gimme H. Walter Lewis j. Wilson michael j. Furlong School of Biological Sciences The University of Queensland St. Lucia Queensland 4072 Australia Cotton Research Unit CSIRO Agriculture Flagship Locked Bag 59 Narrabri NSW 2390 Australia

This study investigated the olfactory responses of 3 thrips species [Frankliniella schultzei Trybom, E occidentalis Pergrande and Thrips tabaci Lindeman （Thysanoptera： Thripidae）] to cotton seedlings [Gossypium hirsutum L. （Malvales： Malvaceae）] simulta- neously damaged by different combinations of herbivores. Cotton seedlings were damaged by foliar feeding Tetranychus urticae Koch （Trombidiforms： Tetranychidae）, Helicoverpa armigera Hibner （Lepidoptera： Noctuidae）, Aphis＇ gossypii Glover （Hemiptera： Aphidi- dae） or root feeding Tenebrio molitor L. （Coleoptera： Tenebrionidae）. Thrips responses to plants simultaneously damaged by 2 species of herbivore were additive and equivalent to the sum of the responses of thrips to plants damaged by single herbivore species feeding alone. For example, E occidentalis was attracted to T. urticae damaged plants but more attracted to undamaged plants than to plants damaged by H. armigera. Plants simultane- ously damaged by low densities of T. urticae and H. armigera repelled E occidentalis but as T. urticae density increased relative to H. armigera density, E occidentalis attraction to coinfested plants increased proportionally. Thrips tabaci did not discriminate between un- damaged plants and plants damaged by H. armigera but were attracted to plants damaged by T. urticae alone or simultaneously damaged by T. urticae and H. armigera. Olfactometer assays showed that simultaneous feeding by 2 herbivores on a plant can affect predator- prey interactions. Attraction of F. occidentalis to plants damaged by its T. urticae prey was reduced when the plant was simultaneously damaged by H. armigera, T. molitor, or A. gossypii and F. schultzei was more attracted to plants simultaneously damaged by T. urticae and H. armigera than to plants damaged by T. urticae alone. We conclude that plant responses to feeding by I species of herbivore are affected by responses to feeding by other herbivores. These plant-mediated interactions between herbivo

关键词： attraction dual-herbivory Frankliniella occidentalis Frankliniella schultzei induced responses predictability simultaneous damage Thrips tabaci

Effect of the postfeeding interval on olfactory responses of thrips to herbivore-induced cotton plants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2016年第6期23卷 881-892页

作者： Rehan Silva Gimme H. Walter Lewis j. Wilson michael j. Furlong School of Biological Sciences University of Queensland St. Lucia Queensland 4072 Cotton Research Unit CSIRO Agriculture Flagship Narrabri NSW 2390 Austrafia

We investigated the responses of 3 thrips species, Frankliniella schultzei Tryborn, E occidentalis Pergrande, and Thrips tabaci Lindeman （Thysanoptera： Thripidae） to herbivore-damaged and undamaged cotton seedlings （Gossypium hirsutum L. [Malvales： Malvaceae]） at a range of time intervals following damage by adult Tetranychus urticae （Koch）, adult T. ludeni （Zacher）（Acari： Tetranychidae） or Helicoverpa armigera （Hubner）（Lepidoptera： Noctuidae） larvae in olfactometer assays. The intensity/frequency of the response of thrips to herbivore-induced plants decreased with time and ultimately disappeared in all cases; however, the rate at which the response declined was related to the herbivore species that inflicted the damage. All 3 species of thrips were attracted to plants damaged by T. urticae for longer than they were to plants damaged by T. ludeni. The duration for which damaged plants remained attractive was also affected by the degree of damage inflicted on cotton seedlings. For example, E schultzei was attracted to plants damaged by a higher density of two-spotted spider mites （100/plant） for much longer than to plants damaged by a lower density of these mites （50/plant）. The results reinforce previous studies that demonstrate that arrangement of variables influences the responses of thrips to their herbivore-induced cotton host plants. Results also show that these responses are variable in time following herbivore damage to cotton plants, which further demonstrates how difficult it is to generalize about the functional significance of these interactions.

关键词： attraction cotton duration induced responses olfactometer Thysanoptera

Tyramides in male alates of black imported fire ants Solenopsis richteri

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2017年第1期24卷 169-172页

作者： j.an Chen michael j. Grodowitz National Biological Control Laboratory Southeast Area United States Department of Agriculture Agriculture Research Service 59 Lee Road Stoneville Mississippi USA

Two species of imported fire ants, the red imported fire ant, Solenopsis invieta Buren, and the black imported fire ant, Solenopsis richteri Forel, were introduced into the United States from South America at the port of Mobile, Alabama in 1918 and 1930s, respectively. Although S. richteri was introduced and established in the United States more than one decade earlier than S. invicta, the latter has gradually displaced S. riehteri throughout most of its distribution.

关键词： SOLENOPSIS richteri FIRE ants ANT behavior ANT ecology ENTOMOLOGY