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Toward genetic transformation of mitochondria in mammalian cells using a recoded drug-resistant selection marker

Journal of Genetics and Genomics 2011年第4期38卷 173-179页

作者： Young Geol Yoon michae. duane.koob Mitochondria Hub Regulation Center and Department of Anatomy and Cell Biology Dong-A University College of Medicine 3-1 Dongdaesin-dongSeo-gu Busan 602-714 Republic of Korea Department of Laboratory Medicine and Pathology and Institute of Human Genetics University of Minnesota Medical School 420 Delaware St. S.E.Minneapolis MN 55455 USA

Due to technical difficulties,the genetic transformation of mitochondria in mammalian cells is still a *** this report,we described our attempts to transform mammalian mitochondria with an engineered mitochondrial genome based on selection using a drug resistance *** the standard drug-resistant neomycin phosphotransferase confers resistance to high concentrations of G418 when targeted to the mitochondria,we generated a recoded neomycin resistance gene that uses the mammalian mitochondrial genetic code to direct the synthesis of this protein in the mitochondria,but not in the nucleus（mitochondrial version）.We also generated a universal version of the recoded neomycin resistance gene that allows synthesis of the drug-resistant proteins both in the mitochondria and *** we transfected these recoded neomycin resistance genes that were incorporated into the mouse mitochondrial genome clones into mouse tissue culture cells by electroporation,no DNA constructs were delivered into the *** found that the universal version of the recoded neomycin resistance gene was expressed in the nucleus and thus conferred drug resistance to G418 selection,while the synthetic mitochondrial version of the gene produced no background drug-resistant cells from nuclear *** recoded synthetic drug-resistant genes could be a useful tool for selecting mitochondrial genetic transformants as a precise technology for mitochondrial transformation is developed.

关键词： Mitochondrial codon Recode Mitochondrial transformation Selection marker G418 Neo^R

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Astrogenesis versus astrogliosis

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Neural Regeneration Research 2017年第2期12卷 203-204页

作者： Andrew O.koob Biology Department and Neuroscience Program University of Hartford West Hartford CT USA

In the mammalian cortex,certain astrocyte subtypes can serve as local adult *** process of local astrogenesis has been linked to astrogliosis,a nebulous term to describe the astrocytic response to injury and *** current evidence indicates that astrogliosis occurs in a context-specific manneralong a graded continuum in response to injury：

关键词： Astrogenesis versus astrogliosis

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Rate analysis of sorption of Ce^3＋,Sm^3＋,and Yb^3＋ions from aqueous solution using Dowex 50W-X8 as a sorbent in a continuous flow reactor

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Journal of Rare Earths 2018年第6期36卷 648-655页

作者： Esmail Monazam Ranjani Siriwardane duane Miller Dustin Mclntyre REM Engineering Services PLLC3537 Collins Ferry Rd.MorgantownWV 26505USA National Energy Technology Laboratory U.S.Department of Energy3610 Collins Ferry Rd.MorgantownWV26507-0880USA AECOM 3610 Collins Ferry Rd.E O.Box 880MorgantownWV 26507-0880USA

Kinetic analysis of removal of three rare earth elements metals, Ce, Sm, and Ybions from aqueous solutions in a continuous flow fixed bed reactor using Dowex 50 W-X8 ion-exchange resin was conducted. The performance of the fixed bed sorption was evaluated using the concept of the sorption breakthrough process. Parameters characteristic of a fixed bed sorption such as breakthrough times,saturation times, critical reactor lengths, and lengths of mass transfer zone were inferred from the metal ion concentration breakthrough curves. The sorption capacity of Dowex 50 W-X8 ion-exchange resin for Ce3+, Sm3+, and Yb3+ are 191, 252, and 294 mg/g, respectively. The sorption kinetics were evaluated using a zero-order, first-order and second-order reaction models. The kinetics of the sorption process follows a zero-order model which has not been reported before. The rate constants of sorption using the zero-order kinetic model are obtained. Two different analysis were conducted to identify whether the diffusion is intraparticle or film diffusion. Both analysis confirms that the film diffusion is the controlling mechanism in reactor bed.

关键词： Rare earth metal ion removal Sorption of rare earths Dowex 50W-X8 for rare earth ion-exchange

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Anion structural effects on interaction of rare earth element ions with Dowex 50W X8 cation exchange resin

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Journal of Rare Earths 2018年第8期36卷 879-890页

作者： duane D.Miller Ranjani Siriwardane Dustin Mcintyre U.S. Department of Energy National Energy Technology Laboratory AECOM

The effect of the anion in the original rare earth element（REE） solution on the reversible ion exchange of Ce nitrate and Ce sulfate with the Dowex 50 W X8 was investigated using attenuated total reflection infrared（ATR-IR） spectroscopy, continuous flow reactor studies coupled with inductively coupled plasma mass spectroscopy（ICP-MS）, and density functional theory（DFT）. The simulated IR spectrum at the DFT B3 LYP/6-31 G（d） level was compared to the experimental results to characterize the IR spectrum, molecular interactions, and bonding of the ion exchanged species. The continuous liquid flow reactor studies show a capacity of 0.72 mmol/g sorbent for the Ce nitrateand 0.96 mmol/g sorbent for the Ce sulfate with the Dowex 50 W X8. The flow reactor studies reveal the type of solute anion（SO_4^（（2-）） or NO_3^（（-））） associated with the REE during cation exchange significantly affects the sorption capacity of the Dowex 50 W X8 ion exchange resin. The calculated REE binding energy（BE） and the DFT optimized structures suggest that the differences in sorption capacity is the result of the formation of different types of partially ionexchanged Ce_2^（（3＋））2 SO_4^（（2-）） and Ce^（（3＋）） 3 NO_3^（（-））. These results suggest that the solute anion affects the equilibrium constants of the Dowex resin by the formation of a charged layer capable of retaining the counterion. Modifying the sulfonic acid site（H＋） in the Dowex 50 W X8 with the NH_4~＋ counterion does not affect the sorption capacity and retention times of the Ce nitrate and Ce sulfate species. These results suggest that the counterions and co-ions having a finite size, may limit access to the Dowex sulfonate active site where the type of REE cation as a nitrate or sulfate in solution may significantly modify the sorption capacity of the ion exchange resin. Similar results are obtained during sorption with nitrates and sulfates of Sm and Yb.

关键词： Ion exchange Dowex Anion effects Rare earth metal sorption

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Symmetry quantification and segmentation in STEM imaging through Zernike moments

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Chinese Physics B 2024年第8期33卷 39-48页

作者： Jiadong Dan Cheng Zhang 赵晓续 N.duane Loh Department of Biological Sciences National University of SingaporeSingapore Center for Bioimaging Sciences(CBIS) National University of SingaporeSingapore Department of Physics National University of SingaporeSingapore School of Materials Science and Engineering Peking UniversityBeijing 100871China

We present a method using Zernike moments for quantifying rotational and reflectional symmetries in scanning transmission electron microscopy(STEM)images,aimed at improving structural analysis of materials at the atomic *** technique is effective against common imaging noises and is potentially suited for low-dose imaging and identifying quantum *** showcase its utility in the unsupervised segmentation of polytypes in a twisted bilayer TaS_(2),enabling accurate differentiation of structural phases and monitoring transitions caused by electron beam *** approach enhances the analysis of structural variations in crystalline materials,marking a notable advancement in the characterization of structures in materials science.

关键词： scanning transmission electron microscopy(STEM) symmetry segmentation

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A topology framework for macromolecular complexes and condensates

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Nano Research 2022年第11期15卷 9809-9817页

作者： Maziar Heidari duane Moes Otto Schullian Barbara Scalvini Alireza Mashaghi Medical Systems Biophysics and Bioengineering Leiden Academic Centre for Drug ResearchFaculty of ScienceLeiden UniversityLeiden 2333CCthe Netherlands Fachbereich Physik Freie Universität BerlinArnimallee 14Berlin 14195Germany Harvard Medical School Harvard University25 Shattuck StBoston MA 02115USA

Macromolecular assemblies such as protein complexes and protein/RNA condensates are involved in most fundamental cellular *** arrangement of subunits within these nano-assemblies is critical for their biological function and is determined by the topology of physical contacts within and between the subunits forming the *** the spatial arrangement of these interactions is of central importance to understand their functional and stability *** this concept article,we propose a circuit topology-based formalism to define the topology of a complex consisting of linear polymeric chains with interand intrachain *** apply our method to a system of model polymer chains as well as protein *** show that circuit topology can categorize different forms of chain *** multi-chain circuit topology should aid analysis and predictions of mechanistic and evolutionary principles in the design of macromolecular assemblies.

关键词： topology macromolecular complex protein evolution folding

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The role of vitamin D in cancer prevention

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Chinese Journal of Natural Medicines 2015年第7期13卷 481-497页

作者： Rachel A.Ness duane D.Miller Wei LI Department of Pharmaceutical Sciences University of Tennessee Health Sciences Center

Vitamin D,also known as cholecalciferol,is the precursor to the active steroid hormone 1,25-dihydroxyvitamin D3(calcitriol; 1,25(OH)2D3).The main physiological role for 1,25(OH)2D3 is to regulate calcium and inorganic phosphate homeostasis for bone *** recently,vitamin D has been investigated for its effects in the prevention and treatment of a variety of diseases such as cancer,autoimmune disorders,and cardiovascular *** data strongly support a role for vitamin D in the prevention of cancer through its anti-proliferative,pro-apoptotic,and anti-angiogenic effects on *** and clinical studies have shown mixed data on the correlation between serum vitamin D levels and cancer *** report seeks to outline results from the most recent preclinical and clinical studies investigating the potential role of vitamin D in cancer prevention.

关键词： Vitamin D Calcitriol Cancer prevention Cancer prognosis Vitamin D deficiency

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Assessment of Dynamic Downscaling of the Extreme Rainfall over East Asia Using a Regional Climate Model

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Advances in Atmospheric Sciences 2011年第5期28卷 1077-1098页

作者：高艳红 Yongkang XUE 彭雯 Hyun—Suk KANG duane WALISER Laboratory for Climate Environment and Disasters of Western ChinaCold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Lanzhou 730000 Department of Geography and Department of Atmospheric and Oceanic Sciences University of California Los Angeles California USA Climate Research Laboratory National Institute of Meteorological Research Korea Meteorological Administration Korea Jet Propulsion Laboratory/California Institute of Technology California USA

This study investigates the capability of the dynamic downscaling method （DDM） in an East Asian climate study for June 1998 using the fifth-generation Pennsylvania State University-National Center for Atmospheric Research non-hydrostatic Mesoscale Model （MM5）.Sensitivity experiments show that MM5 results at upper atmospheric levels cannot match reanalyses data,but the results show consistent improvement in simulating moisture transport at low *** downscaling ability for precipitation is regionally *** the monsoon season over the Yangtze River basin and the pre-monsoon season over North China,the DDM cannot match observed *** Northwest China and the Tibetan Plateau （TP）,where there is high topography,the DDM shows better performance than *** monsoon evolution processes over East Asia,however,are much closer to observational data than *** convection scheme has a substantial impact on extreme rainfall over the Yangtze River basin and the pre-monsoon over North China,but only a marginal contribution for Northwest China and the *** surface parameterizations affect the locations and pattern of rainfall *** 10-day re-initialization in this study shows some improvement in simulated precipitation over some sub-regions but with no obvious improvement in *** setting of the location of lateral boundaries （LLB） westward improves performance of the *** the entire TP in the western model domain improves the DDM performance in simulating precipitation in most *** addition,a seasonal simulation demonstrates that the DDM can also obtain consistent results,as in the June case,even when another two months consist of no strong climate/weather events.

关键词： DDM MM5 cumulus convection scheme land parameterization re-initialization lateral boundary location

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作者： duane Michals.

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Bayesian optimization with active learning of design constraints using an entropy-based approach

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npj Computational Materials 2023年第1期9卷 1866-1879页

作者： Danial Khatamsaz Brent Vela Prashant Singh duane D.Johnson Douglas Allaire Raymundo Arróyave J.Mike Walker’66 Department of Mechanical Engineering Texas A&M UniversityCollege StationTX 77843USA Department of Materials Science and Engineering Texas A&M UniversityCollege StationTX 77843USA Ames Laboratory U.S.Department of EnergyIowa State UniversityAmesIA 50011USA Department of Materials Science&Engineering Iowa State UniversityAmesIA 50011USA Department of Industrial and Systems Engineering Texas A&M UniversityCollege StationTX 77843USA

The design of alloys for use in gas turbine engine blades is a complex task that involves balancing multiple objectives and *** alloys must be ductile at room temperature and retain their yield strength at high temperatures,as well as possess low density,high thermal conductivity,narrow solidification range,high solidus temperature,and a small linear thermal expansion *** Integrated Computational Materials Engineering(ICME)methods are not sufficient for exploring combinatorially-vast alloy design spaces,optimizing for multiple objectives,nor ensuring that multiple constraints are *** this work,we propose an approach for solving a constrained multi-objective materials design problem over a large composition space,specifically focusing on the Mo-Nb-Ti-V-W system as a representative Multi-Principal Element Alloy(MPEA)for potential use in next-generation gas turbine *** approach is able to learn and adapt to unknown constraints in the design space,making decisions about the best course of action at each stage of the *** a result,we identify 21 Pareto-optimal alloys that satisfy all *** proposed framework is significantly more efficient and faster than a brute force approach.

关键词： alloys solidification alloy

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