检索结果-南通市图书馆

2019年中国马铃薯大会

作者： Miles Armstong Xinwei Chen micha bayer Ingo Hein Cell and Molecular Sciences The James Hutton Institute School of Life Sciences The University of Dundee

Potato is the most important non-cereal food crop worldwide and consumed by more than a billion *** and pathogens represent a serious and continuing threat to potato *** of the most widespread and economically significant diseases are late blight,nematodes and *** ability to withstand multiple biotic and abiotic stresses is critical for wild *** the availability of significant germplasm resources including the Commonwealth Potato Collection(CPC)at the James Hutton Institute and improved genomics methods,the potential to exploit natural biodiversity is considerable to secure potatoes as a major food source in the *** the potato genome has provided unprecedented insight into the distribution and organisation of genes that underpin *** have developed a gene-specific target enrichment and sequencing platform,RenSeq,to more rapidly map and clone functional NB-LRR genes that are effective against the major potato *** resistance gene enrichment sequencing(dRenSeq)enables the high-confidence identification and complete sequence validation of functional resistance genes in *** have shown that the technology can direct parental selection in potato breeding programs,confirms transgene integrity in GM crops and enables high-throughput allele *** study reveals a very limited base of utilised resistances in all major potato cultivars but has identified additional and currently very effective resistances in potato varieties that could be combined with the help of the *** combine the most complementary resistances,we can assess the diversity of the Phytophthora *** pathogen populations through a similar enrichment-sequencing based approached referred to as *** combination of dRenSeq and PenSeq enables,for the first time,a comprehensive Pathogen/Crop coevolution study on a global scale.

关键词： RenSeq PenSeq plant-pathogen co-evolution NLR gene deployment

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

SMRT-AgRenSeq-d in potato (Solanum tuberosum) as a method to identify candidates for the nematode resistance Gpa5

引用

Horticulture Research 2023年第11期10卷 271-279页

作者： Yuhan Wang Lynn H.Brown Thomas M.Adams Yuk Woon Cheung Jie Li Vanessa Young Drummond T.Todd Miles R.Armstrong Konrad Neugebauer Amanpreet Kaur Brian Harrower Stan Oome Xiaodan Wang micha bayer Ingo Hein Division of Plant Sciences at the Hutton The University of DundeeErrol RoadInvergowrieDundeeDD25DAUK The James Hutton Institute Errol RoadInvergowrieDundeeDD25DAUK College of Plant Protection China Agricultural UniversityHaidian DistrictBeijing100083China James Hutton Limited The James Hutton InstituteErrol RoadInvergowrieDundeeDD25DAUK Biomathematics and Statistics Scotland Errol RoadInvergowrieDundeeDD25DAUK Crop Research Centre TeagascOak ParkCarlow R93 XE12Ireland HZPC Research B.V.HZPC Edisonweg 58501 XG JoureNetherlands

Potato is the thirdmost important food crop in *** pathogens threaten sustainable crop production but can be controlled,in many cases,through the deployment of disease resistance genes belonging to the family of nucleotide-binding,leucine-rich-repeat(NLR)*** identify effective disease resistance genes in established varieties,we have successfully established SMRT-AgRenSeq in tetraploid potatoes and have further enhanced the methodology by including dRenSeq in an approach that we term *** inclusion of dRenSeq enables the filtering of candidates after the association analysis by establishing a presence/absence matrix across resistant and susceptible varieties that is translated into an F1 *** a SMRT-RenSeq-based sequence representation of the NLRome from the cultivar Innovator,SMRT-AgRenSeq-d analyses reliably identified the late blight resistance benchmark genes Rpi-R1,Rpi-R2-like,Rpi-R3a,and Rpi-R3b in a panel of 117 varieties with variable phenotype *** benchmark genes were identified with an F1 score of 1,which indicates absolute linkage in the *** method also identified nine strong candidates for Gpa5 that controls the potato cyst nematode(PCN)species Globodera pallida(pathotypes Pa2/3).Assuming that NLRs are involved in controlling many types of resistances,SMRT-AgRenSeq-d can readily be applied to diverse crops and pathogen systems.

关键词： resistance linkage crops

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A New Approach to Developing Universally Transferable Markers for Disease Resistance Breeding

A New Approach to Developing Universally Transferable Marker...

引用

2019年中国马铃薯大会

作者： Xinwei Chen Mile R.Armstrong micha bayer Brian Harrower Ingo Hein Cell and Molecular Sciences The James Hutton Institute School of Life Sciences The University of Dundee

Modern molecular technologies enable identification and discrimination of the causal genes for resistance to a variety of pests and *** disease resistance breeding requires efficient tracking and screening for the resistance genes in progenies by employing molecular ***,application of marker assisted selection faces great challenges of limited diagnostics and transferability in potato due to its complex genome of high heterozygosity and *** study aims to tackle the problem through development of a novel type of maker based on diagnostic resistance gene enrichment sequencing(dRenSeq)and assessment for uniqueness of all potential SNPs in comparison with known functional genes across potato *** generated dRenSeq datasets for a total of 136 diverse cultivated and wild potato *** data for three resistance genes R3 a,Nem-Gpa2 and Rx functionally involving in potato resistance to late blight,potato cyst nematode and potato virus X respectively were extracted and *** an extremely stringent threshold with 0% mismatch rate,27,13 and 13 cultivars were detected covering the full reference resistance gene sequences,suggesting presence of the functional resistance genes in these cultivars.A relaxed stringency of 4% mismatch rate was used to explore SNP variations across the dRenSeq panel and identify SNPs with non-resistance nucleotides(NRNs)being universally present in all clones,a criterium adopted for development of universal transferability markers(UTM).One or more SNPs per gene met the criteria as candidate for *** of the candidate SNPs into Kompetitive-allele-specific PCR(KASP)markers faithfully reproduced the dRenseq results and distinguished in 100% success rate clones carrying R-genes from those *** study provides insight into development of UTMs which can be readily extended to other resistance genes and/or different types of functional genes for other traits.

关键词： universally transferrable markers resistance genes marker assisted breeding

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Nonlinear Rydberg exciton-polaritons in Cu2O microcavities

引用

Light(Science & Applications) 2024年第4期13卷 619-627页

作者： Maxim Makhonin Anthonin Delphan Kok Wee Song Paul Walker Tommi Isoniemi Peter Claronino Konstantinos Orfanakis Sai Kiran Rajendran Hamid Ohadi Julian Heckötter Marc Assmann Manfred bayer Alexander Tartakovskii Maurice Skolnick Oleksandr Kyriienko Dmitry Krizhanovskii Department of Physics and Astronomy University of SheffieldSheffield S37RHUK. Department of Physics and Astronomy University of ExeterStocker RoadExeter EX44PYUK. SUPA School of Physics and AstronomyUniversity of St AndrewsSt Andrews KY169SSUK. Fakultät Physik TU DortmundAugust-Schmidt-Straße 444227 DortmundGermany

Rydberg excitons(analogues of Rydberg atoms in condensed matter systems)are highly excited bound electron-hole states with large Bohr *** interaction between them as well as exciton coupling to light may lead to strong optical nonlinearity,with applications in sensing and quantum information ***,we achieve strong effective photon–photon interactions(Kerr-like optical nonlinearity)via the Rydberg blockade phenomenon and the hybridisation of excitons and photons forming polaritons in a Cu2O-filled *** pulsed resonant excitation polariton resonance frequencies are renormalised due to the reduction of the photon-exciton coupling with increasing exciton *** analysis shows that the Rydberg blockade plays a major role in the experimentally observed scaling of the polariton nonlinearity coefficient as∝n4.4±1.8 for principal quantum numbers up to n=*** high principal quantum numbers studied in a polariton system for the first time are essential for realisation of high Rydberg optical nonlinearities,which paves the way towards quantum optical applications and fundamental studies of strongly correlated photonic(polaritonic)states in a solid state system.

关键词： density Rydberg polar

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellulosomal hemicellulases:Indispensable players for ensuring effective lignocellulose bioconversion

引用

Green Carbon 2024年第1期2卷 57-69页

作者： Min Xiao Ya-Jun Liu Edward A.bayer Akihiko Kosugi Qiu Cui Yingang Feng CAS Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China Shandong Provincial Key Laboratory of Synthetic Biology Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China Shandong Engineering Laboratory of Single Cell Oil Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China Shandong Energy Institute Qingdao 266101China Qingdao New Energy Shandong Laboratory Qingdao 266101China University of Chinese Academy of Sciences Beijing 100049China Department of Biomolecular Sciences The Weizmann Institute of ScienceRehovot 7610001Israel Department of Life Sciences and the National Institute for Biotechnology in the Negev Ben-Gurion University of the NegevBeer-Sheva 8499000Israel Biological Resources and Post-harvest Division Japan International Research Center for Agricultural Sciences(JIRCAS)1-1 OhwashiTsukubaIbaraki 305-8686Japan

The bioconversion of lignocellulose has attracted global attention,due to the significant potential of agricultural and forestry wastes as renewable zero-carbon resources and the urgent need for substituting fossil *** cellulosome system is a multi-enzyme complex produced by anaerobic bacteria,which comprises cellulases,hemicellulases,and associated enzymatic and non-enzymatic components that promote biomass *** enhance their efficiency in degrading recalcitrant lignocellulosic matrices,cellulosomes have been employed to construct biocatalysts for lignocellulose bioconversion,such as consolidated bioprocessing and consolidated ***,the second most abundant polysaccharides in plant cell walls,hold valuable application potential but can also induce inhibitory effects on cellulose hydrolysis,thus highlighting the indispensable roles of hemicellulases within the cellulosome *** review evaluated current research on cellulosomal hemicellulases,comparing their types,abundance,and regulation,primarily focusing on eight known cellulosome-producing species of different *** also reviewed their growth conditions,their hemicellulose-degrading capabilities,and the inhibitory effects of hemicellulose on cellulosome-based lignocellulose ***,we proposed strategies for targeted enhancement of hemicellulase in cellulosomes to improve lignocellulose bioconversion in future studies.

关键词： Cellulosome Lignocellulose Hemicellulase Saccharification Biocatalyst

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In situ tumor vaccination with adenovirus vectors encoding measles virus fusogenic membrane proteins and cytokines

引用

World Journal of Gastroenterology 2007年第22期13卷 3063-3070页

作者： Dennis Hoffmann Wibke bayer Oliver Wildner Department of Molecular and Medical Virology Institute of Microbiology and Hygiene Ruhr-University Bochum Bldg. MA Rm. 6/40 D-44801 Bochum Germany

AIM： To evaluate whether intratumoral expression of measles virus fusogenic membrane glycoproteins H and F （MV-FMG）, encoded by an adenovirus vector ***-HI F, alone or in combination with local coexpression of cytokines （IL-2, IL-12, IL-18, IL-21 or GM-CSF）, can serve as a platform for inducing tumor-specific immune responses in colon cancer.METHODS： We used confocal laser scanning microscopy and flow cytometry to analyze cell-cell fusion after expression of MV-FMG by dye colocalization. In a syngeneic bilateral subcutaneous MC38 and Colon26 colon cancer model in C57BL/6 and BALB/c mice, we assessed the effect on both the directly vector-treated tumor as well as the contralateral, not directly vector- treated tumor. We assessed the induction of a tumorspecific cytotoxic T lymphocyte （CTL） response with a lactate dehydrogenase （LDH） release assay.RESULTS： We demonstrated in vitro that transduction of MC38 and Colon26 cells with ***-H/F resulted in dye colocalization, indicative of cell-cell fusion, in addition, in the syngeneic bilateral tumor model we demonstrated a significant regression of the directly vector-inoculated tumor upon intratumoral expression of MV-FMG alone or in combination with the tested cytokines. We observed the highest anti-neoplastic efficacy with MV-FMG and lL-21 coexpression. The degree of tumor regression of the not directly vector-treated tumor correlated with the anti-neoplastic response of the directly vector-treated tumor. This regression was mediated by a tumor-specific CTL response.CONCLUSION： Our data indicate that intratumoral expression of measles virus fusogenic membrane glycoproteins is a promising tool both for direct tumor treatment as well as for tumor vaccination approaches that can be further enhanced by cytokine coexpression.

关键词： Adenovirus vectors Measles virus fusogenic membrane glycoproteins Colorectal cancer Interleukins

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Response of hydrological drought to meteorological drought in the eastern Mediterranean Basin of Turkey

引用

Journal of Arid Land 2021年第5期13卷 470-486页

作者： Türkan bayer ALTIN Bekir N ALTIN Department of Geography Faculty of Science and LettersNiğdeÖmer Halisdemir UniversityNiğde 51240Turkey

The hydrographic eastern Mediterranean Basin of Turkey is a drought sensitive *** basin is an important agricultural area and it is necessary to determine the extent of extreme regional climatic changes as they occur in this ***’s correlation coefficient was used to show the correlation between standardized precipitation index(SPI)and standardized streamflow index(SSI)values on different time *** from five meteorological stations and seven stream gauging stations in four sub-basins of the eastern Mediterranean Basin were analyzed over the period from 1967 to *** correlation between SSI and SPI indicated that in response to meteorological drought,hydrological drought experiences a one-year delay then occurs in the following *** is more evident at all stations from the mid-1990 *** main factor causing hydrological drought is prolonged low precipitation or the presence of a particularly dry *** showed that over a long period(12 months),hydrological drought is longer and more severe in the upper part than the lower part of the *** to SPI-12 values,an uninterrupted drought period is observed from 2002–2003 to 2008–*** indicated that among the drought events,moderate drought is the most common on all timescales in all sub-basins during the past 51 ***-term dry periods with moderate and severe droughts are observed for up to 10 years or more since the late 1990 s,especially in the upper part of the *** precipitation increases in late autumn and early winter,the stream flow also increases and thus the highest and most positive correlation values(0.26–0.54)are found in *** values(ranging between–0.11 and–0.01)are weaker and negative in summer and autumn due to low *** is more evident at all stations in *** relation between hydrological and meteorological droughts is more evident,with the correlation values above 0.50 on longer timescales(12-and 24-month

关键词： meteorological drought hydrological drought standardized precipitation index(SPI) standardized streamflow index(SSI) eastern Mediterranean Basin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Immune-mediated anti-neoplastic effect of intratumoral RSV envelope glycoprotein expression is related to apoptotic death of tumor cells but not to the size of syncytia

引用

World Journal of Gastroenterology 2008年第12期14卷 1842-1850页

作者： Dennis Hoffmann Thomas Grunwald Wibke bayer Oliver Wildner Ruhr-University Bochum Institute of Microbiology and Hygiene Department of Molecular and Medical Virology Bldg. MA Rm. 6/40 Bochum 44801 Germany

AIM： To promote the development of improved tumor vaccination strategies relying on the intratumoral expression of viral fusogenic membrane proteins, we elucidated whether the size of syncytia or the way tumor cells die has an effect on the therapeutic outcome. METHODS： In two syngeneic subcutaneous murine colon cancer models we assessed the anti-neoplastic effect on vector-treated and contralateral untreated tumors. RESULTS： Intratumoral injection of a replication-defective adenovirus encoding respiratory syncytial virus fusion protein （RSV-F） alone （***-F） or together with the attachment glycoprotein RSV-G （***-F/G） led to a significant growth reduction of the vector-treated and contralateral untreated tumors. The treatment response was associated with a strong tumor-specific CTL response and significantly improved survival with medians of 46 d and 44 d, respectively. Intratumoral injection of Ad,RSV-G or a soluble RSV-F encoding adenovirus （***-Fsol） had no significant anti-neoplastic effect. The median survival of these treabnent groups and of ***-treated control animals was about 30 d. CONCLUSION： Although in vitro transduction of colon cancer cell lines with ***-F/G resulted in about 8-fold larger syncytia than with ***-F, the in vivo outcome was not sig nificantly different, Transduction of murinecolon cancer cell lines with *** or ***-F/G caused apoptotic cell death, in contrast to transduction with ***-G or ***-Fsol, suggesting an importance of the mode of cell death. Overall, these findings provide insight into improved tumor vaccination strategies relying on the intratumoral expression of viral fusogenic membrane proteins.

关键词： Adenoviral vectors Tumor vaccination Fuso-genic membrane protein Colorectal cancer Syngeneictumor model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

THERMODYNAMIC ANALYSIS OF THE ENANTIOMER RESOLUTION OF TFA-Ala-OMe, TFA-Ala-Ala-OMe, AND TFA-Ala-Ala-Ala-OMe BY GAS CHROMATOGRAPHY ON CHIRASIL-VAL

引用

Science China Chemistry 1991年第7期34卷 769-776页

作者：林炳承 E.bayer V.MUSCHALEK B.KOPPENHOEFER Dalian Institute of Chemical Physics Academia SinicaDalian 116012 PRC Instisut

TFA-Ala-OMe, TFA-Ala-Ala-OMe, and TFA-Ala-Ala-Ala-OMe can be separated into 1, 2, and 4 enantiomeric pairs, respectively, by GC on the chiral stationary phase Chirasil-Val. The selectivity of the chiral stationary phase for the different stereoisomers is controlled by both interaction enthalpy and interaction entropy. The interaction enthalpy is approximately proportional to the number of Ala units. Although the whole molecule is involved in the interaction, the C-terminus plays an important role. The elution order of DDD- and LLL-TFA-Ala-Ala-Ala-OMe is opposite to the order of the enhalpy values, and the ratio of net retention data t’r(DDD)/t’r(LLL) is increased with increasing temperature. By lowering the temperature, peak crossing of the enantiomeric pair has been observed. Below the isoselective temperature Tiso’ the order of elution is ruled by the enthalpy of interaction, above Tiso by the entropy of interaction.

关键词： gas chromatography enantiomer resolution oligopeptides Chirasil-Val thermodynamic analysis reversal of peak order.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

血小板源性生长因子BB调控缺氧诱导因子1α表达在缺氧性肺动脉高压新生大鼠肺血管重塑中的机制研究

引用

中华新生儿科杂志（中英文） 2024年第3期39卷 168-176页

作者：杨延青巴依尔才次克郭鑫王乐新疆医科大学儿科学院乌鲁木齐830054 新疆医科大学第一附属医院新生儿科乌鲁木齐830054 商丘市中医院儿科商丘476000

目的探讨血小板源性生长因子BB(platelet-derived growth factor-BB,PDGF-BB)是否通过调控缺氧诱导因子1α(hypoxia induced factor-1 alpha,HIF-1α)表达促进肺动脉平滑肌细胞增殖以及参与缺氧性肺动脉高压(hypoxic pulmonary hypertension,HPH)的肺血管重塑。方法160只Wistar新生大鼠按随机数字表法分为常氧组、HPH组、常氧+PDGF-BB组、HPH+PDGF-BB组、HPH+PDGF-BB抑制剂(STI571)组,每组各32只,每组再分为第3、7、14、21天4个亚组,每组8只。PDGF-BB组经尾静脉注射载有PDGF-BB的腺病毒、HPH+STI571组给予STI571灌胃;缺氧建立新生大鼠HPH模型,建模后第3、7、14、21天检测平均右心室收缩压(right ventricular systolic pressure,RVSP),苏木素-伊红染色观察肺小动脉形态变化及检测肺血管重塑指标,免疫组化测定各组PDGF-BB、HIF-1α及增殖相关蛋白核蛋白Ki67在肺血管中的表达部位和表达水平,实时荧光定量聚合酶链反应检测肺组织中PDGF-BB、HIF-1α及Ki67的mRNA水平。结果各时间点HPH组RVSP均高于常氧组(P<0.05),HPH+PDGF-BB组RVSP均高于HPH组(P<0.05),HPH+STI571组RVSP均低于HPH+PDGF-BB组与HPH组(P<0.05);建模后第3天HPH+PDGF-BB组发生肺血管重塑,第7天常氧+PDGF-BB组和HPH组发生肺血管重塑,HPH+STI571组肺血管重塑出现较晚且程度较其他缺氧组轻;第3、7、21天HPH+PDGF-BB组PDGF-BB、HIF-1α、Ki67蛋白及mRNA表达水平均高于其他组(P<0.05),各时间点HPH+STI571组PDGF-BB、HIF-1α、Ki67蛋白及mRNA表达水平均低于HPH+PDGF-BB组和HPH组(P<0.05)。结论PDGF-BB通过上调HIF-1α表达水平促进肺动脉平滑肌细胞增殖、加重肺血管重塑,从而提高HPH新生大鼠肺动脉压力。

关键词：血小板源性生长因子BB 缺氧诱导因子1α 肺动脉高压血管重塑新生大鼠

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：